利用单片段代换系鉴定巴西陆稻IAPAR9中的粒型基因

doi:10.3724/SP.J.1006.2021.02056

Abstract

Abstract:

Rice grain size is a complex quantitative trait controlled by multiple genes. Grain size is an important factor affecting rice yield and quality. The mapping and genetic analysis of genes controlling rice grain size are essential for the concurrent improvement of rice yield and quality. Here 13 QTLs for grain size were detected using 153 rice single-segment substitution lines in rice, which were derived from HJX74 as the receptor parent, and IAPAR9 as the donor parent. One-way ANOVA and Duncan’s multiple comparison were employed to detect the genetic bases of rice grain size in two consecutive years. Based on the substitution mapping using overlapped substitution-fragment in the SSSLs, a total of 13 grain size-related QTLs were detected on chromosomes 1, 2, 4, 5, 6, 7, 9, and 11, including nine QTLs controlling grain length, one QTL controlling grain width, and three QTLs controlling 1000-grain weight. Furthermore, qGL1-2, qTGW1-2, and qGL11 were novel identified QTLs. This study provided new basis for cloning and functional analysis of genes regulating grain size.

Key words: rice, single segment substitution line, grain size, QTL mapping

ZHANG Bo, PEI Rui-Qing, YANG Wei-Feng, ZHU Hai-Tao, LIU Gui-Fu, ZHANG Gui-Quan, WANG Shao-Kui. Mapping and identification QTLs controlling grain size in rice (Oryza sativa L.) by using single segment substitution lines derived from IAPAR9[J].Acta Agronomica Sinica, 2021, 47(8): 1472-1480.

Figures/Tables 8

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标记名称 Marker	基因 Gene	序列 Sequence (5′-3′)	标记名称 Marker	基因 Gene	序列 Sequence (5′-3′)
xp14F	LGY3	TGTCAACAGAAACCAGCAAAA	RMw513F	GW5	GTATTTGTTTGTCGCATTC
xp14R		TGGTCGATCAATTCCTCTGC	RMw513R		TAGGACCATAGATGTGAGTTA
GS3R1	gs3	AGGCTGGCTTACTCGCTG	RM574F	GS5	GGCGAATTCTTTGCACTTGG
GS3R2		CAGCAGGCTGGCTTACTCTATT	RM574R		ACGGTTTGGTAGGGTGTCAC
GS3F		CTGTATATATATTTCTTGCAGGGTG	Indel-1F	GW7	CCATAGTAAGACGACCTT
RM186F	GL3.1	TCCTCCATCTCCTCCGCTCCCG	Indel-1R		GATATTCTGTCAGCAGTT
RM186R		GGGCGTGGTGGCCTTCTTCGTC	PSM710F	gw8	GCCAGCCAAGAAAAGCGACA
			PSM710R		TCTTGAGATCCCACTCCATG

染色体 Chr.	单片段材料数量 No. of SSSLs	代换片段总长度 TL (cM)	代换片段平均长度 CL (cM)	代换片段覆盖率 CP (%)
Chr. 1	30	571.00	19.03	59.16
Chr. 2	22	526.00	23.91	78.31
Chr. 3	4	112.40	28.10	35.21
Chr. 4	14	380.70	27.19	43.90
Chr. 5	12	179.30	14.94	53.98
Chr. 6	24	541.40	22.56	73.55
Chr. 7	7	75.20	10.74	19.27
Chr. 8	8	181.40	22.68	66.91
Chr. 9	9	256.70	28.52	85.03
Chr. 10	6	160.60	26.77	55.93
Chr. 11	16	320.60	20.04	60.04
Chr. 12	1	6.90	6.90	3.15
平均Mean	12.75	276.02	20.95	52.87

性状 Trait	位点 QTL	染色体 Chr.	区间 Marker interval	加性效应Additive effect		P值P-value
性状 Trait	位点 QTL	染色体 Chr.	区间 Marker interval	2018	2019	2018	2019
粒长Grain length	qGL1-1	1	PSM13-RM84	-0.19	-0.11	0.0025	0.0188
	qGL1-2	1	OSR23-RM104	0.30	0.31	0.0001	0.0005
	qGL2	2	RM250-RM166	-0.19	-0.15	<0.0001	0.0029
	qGL4	4	RM317-PSM110	-0.23	-0.15	0.0002	0.0081
	qGL5	5	RM164-RM161	-0.19	-0.12	0.0002	0.0289
	qGL6	6	PSM136-RM275	-0.22	-0.15	<0.0001	0.0227
	qGL7	7	RM505-PSM432	0.37	0.43	0.0003	<0.0001
	qGL9	9	PSM399-RM105	-0.20	-0.15	<0.0001	0.0084
	qGL11	11	RM209-RM21	-0.17	-0.16	0.0005	0.0023
粒宽Grain width	qGW2	2	RM263-RM525	0.09	0.08	0.0003	0.0039
千粒重1000-grain weight	qTGW1-1	1	PSM13-RM84	-1.46	-1.32	<0.0001	0.0016
千粒重1000-grain weight	qTGW1-2	1	OSR23-RM104	0.65	1.25	0.0003	0.0011
	qTGW4	4	RM127-RM280	-1.31	-1.45	<0.0001	<0.0001

Mapping and identification QTLs controlling grain size in rice (Oryza sativa L.) by using single segment substitution lines derived from IAPAR9

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