绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选

doi:10.3724/SP.J.1006.2022.04283

Abstract

Abstract:

Soil salinization has become one of the important factors affecting agricultural production in China. It is of great significance to screen germplasm resources of mungbean [Vigna radiate (L.) Wilczek] for the utilization of salinized land. In the present study, the seedlings of 346 domestic and foreign collections of mungbean were exposed to 150 mmol L ^-1 NaCl. To evaluate the salt-tolerance of different mungbean genotypes at seedling stage, 12 indicators were calculated, including plant height, fresh weight of above-ground part, fresh weight of root, dry weight of above-ground part, dry root weight, root length, and root volume. Salt tolerance of each sample was comprehensively evaluated and classified by principal component analysis, membership function analysis, salt tolerance comprehensive evaluation, and cluster analysis; and the regression equation of salt tolerance prediction was established by stepwise regression analysis. The results revealed that there were significant differences between treatment group and control group in each trait evaluation index, and the salt tolerance coefficients of the 12 indicators were correlated to some content. Combining the classification of salt damage symptoms and the comprehensive evaluation results of salt tolerance, 26 mungbean germplasms with high salt tolerance, 65 germplasms with high salt tolerance, 74 germplasms with salt sensitive, and 18 germplasms with extremely salt sensitive were selected at seedling stage. Among them, 10 varieties such as C04125 from Jiangxi and C06310 from the Philippines had the strongest salt tolerance, which could be used as excellent resources for mungbean salt tolerance breeding. Above-ground fresh weight, root fresh weight, root dry weight, root length, root volume, and root branch number could be used to predict salt tolerance of mungbean at seedling stage.

Key words: mungbean [Vigna radiate (L.) Wilczek], germplasm resources, seeding stage, salt tolerance, comprehensive evaluation

HU Liang-Liang, WANG Su-Hua, WANG Li-Xia, CHENG Xu-Zhen, CHEN Hong-Lin. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage[J].Acta Agronomica Sinica, 2022, 48(2): 367-379.

Figures/Tables 8

Table 1

Fig. 1

Fig. 2

Table 2

Table 3

Table 4

Table 5

D-value of salt tolerance of different genotypes of mungbean"

品种 Variety	D值 D-value	排名 Rank	品种 Variety	D值 D-value	排名 Rank	品种 Variety	D值 D-value	排名 Rank	品种 Variety	D值 D-value	排名 Rank
C05290	0.663	164	C03069	0.592	253	C06292	0.741	44	C06227	0.568	270
C05495	0.747	36	C03070	0.549	283	C06293	0.758	21	C06228	0.541	291
C05506	0.683	126	C03071	0.708	96	C06294	0.699	105	C06230	0.663	165
C05668	0.678	135	C03072	0.602	241	C06295	0.703	99	C06231	0.406	340
C05786	0.513	308	C03078	0.609	232	C06296	0.769	12	C06232	0.547	285
C05787	0.762	19	C03079	0.702	101	C06297	0.757	23	C06233	0.502	313
C05798	0.526	300	C03086	0.621	223	C06298	0.538	293	C06234	0.453	331
C06117	0.748	35	C03090	0.669	151	C06299	0.792	4	C06235	0.562	275
C06118	0.626	219	C03094	0.740	47	C06300	0.413	339	C06236	0.724	65
C05788	0.722	67	C03140	0.720	75	C06302	0.740	46	C06237	0.663	162
C06397	0.631	210	C03606	0.538	294	C06303	0.789	5	C06238	0.643	188
C06396	0.664	159	C03656	0.717	88	C06304	0.458	328	C06241	0.642	190
C06386	0.632	208	C03608	0.545	289	C06305	0.636	201	C06242	0.341	343
C05636	0.674	140	C03609	0.772	8	C06306	0.727	60	C06243	0.631	211
C06398	0.711	94	C03610	0.754	28	C06307	0.615	229	C06244	0.582	261
C06390	0.746	38	C03601	0.768	13	C06308	0.756	25	C06246	0.561	277
C06391	0.744	39	C03651	0.682	128	C06309	0.547	286	C06247	0.664	161
C05503	0.583	260	C03273	0.755	26	C06310	0.804	2	C06248	0.607	236
C06392	0.717	83	C03279	0.777	7	C06311	0.721	71	C06249	0.522	301
C06393	0.796	3	C03280	0.719	78	C06312	0.743	40	C06251	0.657	173
C06394	0.751	31	C03284	0.733	56	C06313	0.740	48	C06253	0.575	266
C06383	0.567	271	C03289	0.638	197	C06314	0.738	50	C06254	0.477	321
C06384	0.672	146	C03304	0.670	149	C06315	0.673	145	C06255	0.719	77
C05249	0.507	311	C03305	0.432	336	C06316	0.721	69	C06256	0.533	295
C05248	0.726	62	C03312	0.695	108	C06317	0.695	106	C06257	0.643	189
C06385	0.664	160	C03316	0.741	45	C06318	0.454	329	C06258	0.717	86
C04717	0.720	76	C03319	0.695	107	C06319	0.638	196	C06259	0.512	309
C06387	0.736	54	C03320	0.680	131	C06320	0.501	314	C06260	0.662	167
C06389	0.657	175	C03334	0.715	91	C06321	0.462	327	C06261	0.437	334
C06388	0.740	49	C03380	0.748	34	C06322	0.596	248	C06262	0.520	302
C05635	0.751	30	C03381	0.674	138	C06323	0.750	32	C06263	0.454	330
C05637	0.715	92	C03383	0.685	120	C06324	0.653	179	C06264	0.516	306
C06402	0.716	90	C03384	0.639	195	C06325	0.607	234	C06265	0.648	185
C03413	0.684	121	C03385	0.721	70	C06326	0.699	104	C06266	0.588	257
C03416	0.721	72	C03397	0.472	323	C06327	0.607	235	C06267	0.432	335
C06395	0.685	119	C03399	0.684	123	C06328	0.584	259	C06268	0.741	43
C06405	0.547	284	C03400	0.769	11	C06329	0.500	316	C06269	0.466	325
C05179	0.701	103	C03401	0.590	256	C06330	0.615	228	C06270	0.770	10
C06179	0.562	276	C03402	0.768	15	C06331	0.718	81	C06271	0.645	187
C06403	0.665	158	C03405	0.723	66	C06332	0.768	14	C06272	0.618	226
C06404	0.725	63	C03434	0.674	141	C06333	0.720	74	C06273	0.576	264
C03414	0.635	202	C03435	0.684	122	C06334	0.683	124	C06274	0.667	155
C04702	0.687	116	C03436	0.725	64	C06335	0.682	127	C06275	0.566	274
C03923	0.759	20	C03440	0.757	22	C06336	0.547	287	C06276	0.717	89
C00376	0.573	267	C03441	0.547	288	C06337	0.633	204	C06277	0.711	93
C00377	0.681	130	C03442	0.593	251	C06338	0.722	68	C06278	0.658	172
C00381	0.637	199	C03471	0.754	29	C06339	0.718	82	C06279	0.476	322
C00385	0.516	304	C03472	0.702	102	C06340	0.632	206	C06280	0.552	281
C00389	0.554	279	C03484	0.632	207	C06341	0.479	320	C05900	0.566	273
C00391	0.707	97	C03485	0.667	156	C06342	0.778	6	C05901	0.617	227
C00396	0.593	250	C03486	0.683	125	C06343	0.597	246	C05902	0.678	134
C00399	0.494	317	C03526	0.673	144	C06344	0.464	326	C05903	0.444	333
C00834	0.592	254	C03527	0.676	137	C06345	0.612	230	C05904	0.721	73
C00840	0.630	212	C03528	0.541	290	C06346	0.569	269	C05905	0.651	182
C00848	0.742	41	C03529	0.444	332	C06347	0.717	85	C05906	0.640	194
C00858	0.693	111	C03530	0.694	110	C06348	0.692	113	C05907	0.632	205
C01606	0.592	255	C03593	0.619	225	C06349	0.567	272	C05908	0.659	171
C01607	0.604	239	C03594	0.681	129	C06350	0.690	115	C05909	0.665	157
C01609	0.671	147	C03595	0.667	154	C06351	0.630	213	C05910	0.577	263
C01610	0.692	114	C03596	0.677	136	C06352	0.596	247	C05911	0.608	233
C01611	0.594	249	C03597	0.603	240	C06353	0.619	224	C05912	0.628	218
C01623	0.651	183	C03598	0.625	220	C06200	0.650	184	C05913	0.491	318
C01627	0.655	177	C03599	0.766	16	C06201	0.571	268	C05914	0.647	186
C01630	0.528	299	C04081	0.514	307	C06202	0.728	59	C05915	0.610	231
C01633	0.601	242	C04082	0.641	193	C06203	0.755	27	C05916	0.662	166
C01635	0.764	17	C04083	0.490	319	C06204	0.660	170	C05917	0.736	52
C01651	0.718	80	C04084	0.735	55	C06205	0.653	178	C05918	0.679	133
C01657	0.668	153	C04085	0.652	180	C06206	0.173	346	C05919	0.635	203
C01660	0.605	238	C04086	0.703	100	C06208	0.529	298	C05920	0.657	174
C01740	0.517	303	C04125	0.829	1	C06209	0.670	150	C05921	0.652	181
C01741	0.628	215	C04126	0.540	292	C06210	0.587	258	C05922	0.763	18
C01742	0.692	112	C04127	0.637	198	C06211	0.468	324	C05923	0.661	168
C01751	0.624	221	C04558	0.750	33	C06212	0.674	143	C05924	0.516	305
C01752	0.770	9	C04559	0.710	95	C06213	0.717	87	C05925	0.674	139
C01753	0.628	216	C04560	0.680	132	C06214	0.599	244	C05926	0.600	243
C01754	0.599	245	C04561	0.741	42	C06215	0.503	312	C05927	0.674	142
C01755	0.706	98	C06281	0.624	222	C06216	0.687	117	C05928	0.660	169
C01815	0.606	237	C06282	0.593	252	C06217	0.552	282	C05929	0.377	341
C02056	0.694	109	C06283	0.641	192	C06218	0.670	148	C05930	0.341	342
C02058	0.575	265	C06284	0.727	61	C06219	0.501	315	C05931	0.731	57
C02071	0.746	37	C06285	0.669	152	C06220	0.561	278	C05932	0.552	280
C02072	0.686	118	C06286	0.736	53	C06221	0.426	338	C05933	0.532	296
C02073	0.663	163	C06287	0.656	176	C06222	0.637	200	C05934	0.718	79
C02077	0.212	345	C06288	0.736	51	C06223	0.731	58	C05935	0.292	344
C02078	0.628	217	C06289	0.431	337	C06224	0.641	191	C05937	0.630	214
C02081	0.632	209	C06290	0.756	24	C06225	0.507	310
C02103	0.717	84	C06291	0.579	262	C06226	0.531	297

Table 5

Fig. 3

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等级 Grade	耐盐性 Salt tolerance	表型特征 Symptom
1	高耐盐 Highly salt-tolerant	生长正常, 未出现盐害症状。 Normal growth, no symptoms were observed.
3	耐盐 Salt-tolerant	生长基本正常, 少数叶片边缘出现青枯或卷缩。 The growth is basically normal, and a few leaves appear withered or curled on the edge.
5	中耐盐 Mediumly salt-tolerant	叶片面积一半左右出现青枯或卷缩。 About half of the leaf area appear withered or curled.
7	敏感 Salt-sensitive	叶片大部分面积干枯坏死, 生长严重受阻。 Most of the leaves are dry and necrotic, and the growth is severely blocked.
9	高敏感 Highly salt-sensitive	严重受害, 植株完全枯萎。 Severely damaged, the plant is completely withered.

性状 Trait	最小值 Min.	最大值 Max.	平均值 Mean	标准差 SD	变异系数 CV (%)
PH	0.355	0.998	0.730	0.14	19.08
AFW	0.159	1.104	0.596	0.23	39.42
RFW	0.113	1.044	0.684	0.22	32.30
ADW	0.206	1.077	0.711	0.19	26.54
RDW	0.220	0.998	0.713	0.19	26.08
RL	0.156	1.026	0.633	0.24	37.86
RSA	0.148	1.134	0.719	0.21	28.62
RAD	0.574	1.144	0.873	0.08	9.61
RLPV	0.156	1.053	0.630	0.24	37.61
RV	0.140	1.085	0.796	0.18	22.73
RT	0.049	0.998	0.647	0.27	42.11
RF	0.112	0.999	0.607	0.24	40.12

性状 Trait	PH	AFW	RFW	ADW	RDW	RL	RSA	RAD	RLPV	RV	RT
AFW	0.53^*
RFW	0.17	0.40^*
ADW	0.64^*	0.74^**	0.21
RDW	0.45^*	0.63^*	0.75^**	0.61^*
RL	0.46^*	0.40^*	0.40^*	0.44^*	0.52^*
RSA	0.31	0.39	0.46^*	0.35	0.52^*	0.93^**
RAD	-0.58^*	-0.21	-0.06	-0.40^*	-0.24	-0.57^*	-0.26
RLPV	0.46^*	0.40^*	0.40^*	0.44^*	0.52^*	0.99^**	0.93^**	-0.57^*
RV	0.08	0.30	0.46^*	0.19	0.44^*	0.71^**	0.91^**	0.13	0.71^**
RT	0.38	0.34	0.18	0.37	0.35	0.72^**	0.60^*	-0.57^*	0.72^**	0.38
RF	0.37	0.34	0.35	0.38	0.44^*	0.90 ^**	0.87^**	-0.45^*	0.90^**	0.69^*	0.66^*

性状 Trait	主成分因子 Comprehensive factors
性状 Trait	F1	F2	F3	F4	F5
PH	-0.22	0.46	0.10	-0.04	-0.57
AFW	-0.23	0.38	-0.24	-0.37	0.39
RFW	-0.19	-0.02	-0.53	0.55	0.07
ADW	-0.23	0.48	-0.10	-0.34	0.04
RDW	-0.26	0.19	-0.41	0.36	0.01
RL	-0.38	-0.15	0.15	0.02	-0.10
RSA	-0.36	-0.28	-0.03	-0.13	-0.12
RAD	0.20	-0.26	-0.51	-0.43	0.03
RLPV	-0.38	-0.15	0.15	0.02	-0.10
RV	-0.37	-0.39	-0.25	-0.31	-0.13
RT	-0.30	-0.04	0.31	0.08	0.67
RF	-0.37	-0.19	0.13	-0.04	-0.07
特征值Eigen values	6.29	2.01	1.59	0.82	0.45
贡献率Contribution rate (%)	52.39	16.78	13.24	6.80	3.71
累计贡献率Accumulative contribution rate (%)	52.39	69.18	82.41	89.21	92.93

Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage

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