生长素调控水稻颖花开放的效应研究

doi:10.3724/SP.J.1006.2023.22027

Abstract

Abstract:

Floret opening which is driven by swelling of lodicules has a direct effect on rice pollination and fertilization. Auxin is an essential phytohormone in regulating reproductive development processes such as anther dehiscence, pollen fertility, and seed initiation. To elucidate the role of auxin in floret opening, the effects of exogenous auxin and its inhibitors on floret opening, and dynamic changes of endogenous auxin levels, and gene relative expression levels of auxin pathway in florets and lodicules were investigated in japonica cultivar Zhonghua 11. The results showed that the panicles soaked with IAA (10-20 mmol L^-1) or NAA (0.05-0.50 mmol L^-1) delayed significantly floret opening. Under high concentration of NAA (0.5 mmol L^-1), compared with the water-treated panicles, the floret opening was postponed by three days. The prolonged opening duration of single floret and decreased seed-setting rate were also observed after IAA and NAA treatments. Treatments with IAA polar transport inhibitor TIBA and function inhibitor PCIB delayed florets opening as well. Furthermore, the application of methyl jamonate (MeJA) could restore effectively floret opening which was retared by NAA pretreatments. A sharp decline of IAA levels was detected two hours before floret opening in natural condition. Compared to that at 1 d and 2 d before opening, IAA level in florets at 2 hours before opening was decreased by 65.85% and 74.27%, respectively. Corresponding to the changes of IAA levels in florets, the relative expression levels of IAA biosynthetic genes (OsTAR2, OsYUCCA3/4/8) in the lodicules were down-regulated during floret opening, while the expressions of catabolic genes (OsGH3.2/OsGH3.8) in formation of inactive IAA conjugates, IAA efflux transport genes (OsPIN1, OsPIN1a), and its positive regulator BG1 gene were significantly up-regulated. 13 differentially expressed early auxin response genes (OsAUX/IAAs, OsARF15, OsSAURs) were also identified, among which, 10 were up-regulated and 3 down-regulated. In conclusion, rice floret opening was regulated by endogenous auxin, but was inhibited by elevating auxin level in lodicules.

Key words: rice, floret opening, auxin, inhibitory effect

HE Yong-Ming, ZHANG Fang. Study of regulating effect of auxin on floret opening in rice[J].Acta Agronomica Sinica, 2023, 49(6): 1690-1698.

Figures/Tables 6

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