Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2025, Vol. 51 ›› Issue (5): 1400-1408.doi: 10.3724/SP.J.1006.2025.44145

• RESEARCH NOTES • Previous Articles     Next Articles

Genome-wide associate analysis of flowering traits and identification of candidate genes in peanut

LI Wen-Jia1,2(), LIAO Yong-Jun2, HUANG Lu2, LU Qing2, LI Shao-Xiong2, CHEN Xiao-Ping2, JIN Jing-Wei1,*(), WANG Run-Feng2,*()   

  1. 1College of Grassland Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China
    2Crops Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Crop Genetic Improvement / South China Peanut Sub-Centre of National Centre of Oilseed Crops Improvement, Guangzhou 510640, Guangdong, China
  • Received:2024-09-04 Accepted:2024-12-12 Online:2025-05-12 Published:2024-12-19
  • Contact: *E-mail: wangrunfeng@gdaas.cn; E-mail: jinjingweisoil2008@163.com
  • Supported by:
    Guangzhou Basic and Applied Basic Research Foundation(2023A04J0776);Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences(202104TD);China Agriculture Research System of MOF and MARA(CARS-13)

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Abstract:

The flowering time, one of the important indexes to evaluate the early maturing varieties of peanut, has an important impact on the yield. It is very important to mine the genetic loci related to peanut flowering time and screen candidate genes for early maturation breeding of peanut. In this paper, the flowering time of 390 peanut germplasms in five environments was evaluated. Phenotypic statistics showed that the variation coefficient of peanut flowering time ranged from 3.21% to 8.54% in five environments, and there were abundant phenotypic variations. The flowering time of peanut basically showed normal distribution and was greatly affected by the environment. A total of 259 SNPs significantly associated with flowering time were identified by genome-wide association analysis. Of them, 29 were identified in more than two environments, and located on chromosomes A01, A02, A03, A04, A05, A07, A10, B01, B02, B03, B04, B06, B07, and B09, it can explain 3.47% to 8.58% of the phenotypic variation. After searching for candidate genes in the confidence intervals of 29 recurrent loci upstream and downstream 100 kb, 159 genes with functional annotations were found. Seven candidate genes related to peanut flowering time were predicted in the candidate interval, encoding R2R3-MYB transcription factor, chlorophyll a-b binding protein, bHLH transcription factor, WRKY transcription factor and FAR1 transcription factor, respectively. The results of this study can lay a foundation for understanding the genetic mechanism of peanut flowering regulation and peanut early maturation breeding.

Key words: peanut, flowering time, genome-wide association analysis, candidate gene, early maturation breeding

Table 1

Statistical analysis of flowering time of peanut populations"

环境
Environment		 均值
Mean		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis		 最小值
Min.		 最大值
Max.		 变异系数
CV (%)
2022E 45.14 2.08 1.70 4.02 41.44 54.88 4.62
2022L 25.38 1.56 1.53 3.78 22.18 33.00 6.16
2023E 47.57 1.53 1.18 1.84 44.71 53.09 3.21
2023L 28.98 2.48 0.38 -0.56 24.12 35.00 8.54
2024E 44.41 1.90 2.13 5.78 42.00 54.56 4.29
BLUE 32.67 1.60 1.65 3.43 30.09 39.45 4.90

Fig. 1

Phenotypic distribution of flowering time in peanut populations Abbreviations are the same as those given in Table 1."

Fig. 2

Correlation analysis of flowering time of peanut population in different environments Abbreviations are the same as those given in Table1. ** and *** indicate significant correlation at the 0.01 and 0.001 levels, respectively."

Fig. 3

Manhattan and QQ plot diagrams for genome-wide association analysis of peanut flowering time Abbreviations are the same as those given in Table1. The red line indicates -log10(P) = 6."

Table S1

Significant SNPs associated with flowering time in peanut"

标记
SNP		 染色体
Chromosome		 位置
Position		 P值
P-value		 表型变异贡献率
Phenotype variation explained (%)		 环境
Environment
CM014315.1_465100 A01 465,100 7.40E-07 3.59 2022L
CM014315.1_15392620 A01 15,392,620 2.77E-08 6.01 2022E
CM014315.1_23513485 A01 23,513,485 7.74E-07 3.48 BLUE
CM014315.1_48457374 A01 48,457,374 3.57E-08 5.91 2022E
CM014315.1_62078485 A01 62,078,485 6.66E-07 3.62 2022L
CM014315.1_62078488 A01 62,078,488 6.66E-07 3.62 2022L
CM014315.1_62078492 A01 62,078,492 6.66E-07 3.62 2022L
CM014315.1_62078493 A01 62,078,493 6.66E-07 3.62 2022L
CM014315.1_63737330 A01 63,737,330 2.78E-07 3.88 2022L
CM014315.1_77174051 A01 77,174,051 6.13E-07 3.64 2022L
CM014315.1_77174051 A01 77,174,051 9.43E-07 4.83 2024E
CM014315.1_105876877 A01 105,876,877 1.63E-07 4.03 2022L
CM014315.1_105876877 A01 105,876,877 6.94E-07 4.87 2023E
CM014315.1_105876890 A01 105,876,890 2.38E-07 3.82 BLUE
CM014315.1_105910474 A01 105,910,474 9.40E-08 5.53 2022E
CM014316.1_2235615 A02 2,235,615 8.90E-08 5.78 2024E
CM014316.1_2235621 A02 2,235,621 8.90E-08 5.78 2024E
CM014316.1_3004235 A02 3,004,235 1.98E-08 6.39 2024E
CM014316.1_6278118 A02 6,278,118 2.76E-08 6.01 2022E
CM014316.1_6374345 A02 6,374,345 5.52E-07 4.84 2022E
CM014316.1_6385154 A02 6,385,154 5.52E-07 4.84 2022E
CM014316.1_6401193 A02 6,401,193 5.52E-07 4.84 2022E
CM014316.1_6454703 A02 6,454,703 5.52E-07 4.84 2022E
CM014316.1_6463338 A02 6,463,338 1.67E-07 3.92 BLUE
CM014316.1_6463338 A02 6,463,338 6.24E-07 4.99 2024E
CM014316.1_6474128 A02 6,474,128 3.84E-08 5.88 2022E
CM014316.1_6524469 A02 6,524,469 5.52E-07 4.84 2022E
CM014316.1_6562935 A02 6,562,935 7.20E-07 4.74 2022E
CM014316.1_6569396 A02 6,569,396 8.44E-07 4.68 2022E
CM014316.1_6629539 A02 6,629,539 3.37E-07 5.24 2024E
CM014316.1_6677102 A02 6,677,102 7.34E-07 4.73 2022E
CM014316.1_6691941 A02 6,691,941 7.34E-07 4.73 2022E
CM014316.1_6703005 A02 6,703,005 7.34E-07 4.73 2022E
CM014316.1_6762900 A02 6,762,900 7.36E-07 4.73 2022E
CM014316.1_6785411 A02 6,785,411 7.36E-07 4.73 2022E
CM014316.1_6789784 A02 6,789,784 3.38E-07 5.03 2022E
CM014316.1_6809741 A02 6,809,741 7.36E-07 4.73 2022E
CM014316.1_7287153 A02 7,287,153 1.41E-07 5.37 2022E
CM014316.1_7287325 A02 7,287,325 1.41E-07 5.37 2022E
CM014316.1_17191238 A02 17,191,238 7.82E-08 4.14 BLUE
CM014316.1_37973138 A02 37,973,138 6.02E-11 6.26 BLUE
CM014316.1_37973138 A02 37,973,138 8.30E-07 4.88 2024E
CM014316.1_48270583 A02 48,270,583 2.09E-07 3.86 BLUE
CM014316.1_98564085 A02 98,564,085 7.73E-07 3.48 BLUE
CM014316.1_108415738 A02 108,415,738 3.99E-07 3.67 BLUE
CM014317.1_9657242 A03 9,657,242 8.18E-07 3.56 2022L
CM014317.1_10749415 A03 10,749,415 3.13E-08 6.20 2024E
CM014317.1_36360729 A03 36,360,729 1.87E-07 3.99 2022L
CM014317.1_72568977 A03 72,568,977 4.38E-07 3.65 BLUE
CM014317.1_94326989 A03 94,326,989 2.13E-07 3.85 BLUE
CM014317.1_101165971 A03 101,165,971 4.35E-07 5.14 2024E
CM014317.1_106906399 A03 106,906,399 3.18E-07 5.06 2022E
CM014317.1_107129196 A03 107,129,196 1.90E-07 5.26 2022E
CM014317.1_107233483 A03 107,233,483 1.23E-07 5.42 2022E
CM014317.1_107565796 A03 107,565,796 2.92E-08 5.99 2022E
CM014317.1_107936173 A03 107,936,173 5.74E-08 5.72 2022E
CM014317.1_108128031 A03 108,128,031 3.26E-08 5.95 2022E
CM014317.1_108275948 A03 108,275,948 3.00E-07 5.08 2022E
CM014317.1_108278090 A03 108,278,090 9.38E-08 5.53 2022E
CM014317.1_109103081 A03 109,103,081 1.56E-07 5.33 2022E
CM014317.1_110113252 A03 110,113,252 9.20E-07 3.43 BLUE
CM014317.1_110152517 A03 110,152,517 9.00E-07 4.65 2022E
CM014317.1_110323131 A03 110,323,131 5.80E-07 4.82 2022E
CM014317.1_110379585 A03 110,379,585 3.16E-07 5.06 2022E
CM014317.1_110652469 A03 110,652,469 3.01E-07 5.08 2022E
CM014317.1_111533879 A03 111,533,879 9.63E-07 4.63 2022E
CM014317.1_111757227 A03 111,757,227 7.65E-07 4.72 2022E
CM014317.1_112179892 A03 112,179,892 2.56E-07 5.14 2022E
CM014317.1_112327535 A03 112,327,535 9.14E-09 6.45 2022E
CM014317.1_112890983 A03 112,890,983 9.15E-07 4.65 2022E
CM014317.1_112891842 A03 112,891,842 2.01E-07 5.23 2022E
CM014317.1_112922024 A03 112,922,024 4.48E-07 4.92 2022E
CM014317.1_112991602 A03 112,991,602 6.72E-07 4.77 2022E
CM014317.1_113113202 A03 113,113,202 9.07E-07 4.65 2022E
CM014317.1_113288170 A03 113,288,170 3.13E-07 5.18 2023E
CM014317.1_113545811 A03 113,545,811 7.88E-07 4.71 2022E
CM014317.1_113602281 A03 113,602,281 7.12E-07 4.75 2022E
CM014317.1_113686216 A03 113,686,216 4.25E-07 4.94 2022E
CM014317.1_113783685 A03 113,783,685 4.67E-07 4.91 2022E
CM014317.1_113914790 A03 113,914,790 9.11E-09 4.76 BLUE
CM014317.1_113914790 A03 113,914,790 5.58E-07 5.04 2024E
CM014317.1_114828598 A03 114,828,598 5.50E-07 4.96 2023E
CM014317.1_115354759 A03 115,354,759 3.88E-07 5.10 2023E
CM014317.1_115379489 A03 115,379,489 2.50E-07 5.27 2023E
CM014317.1_115657511 A03 115,657,511 7.40E-10 7.63 2023E
CM014317.1_115657511 A03 115,657,511 6.69E-09 4.86 BLUE
CM014317.1_115657511 A03 115,657,511 7.57E-09 6.79 2024E
CM014317.1_115657511 A03 115,657,511 5.04E-08 5.77 2022E
CM014317.1_115657511 A03 115,657,511 1.05E-07 4.16 2022L
CM014317.1_115712510 A03 115,712,510 7.21E-07 3.60 2022L
CM014317.1_115713539 A03 115,713,539 9.71E-09 6.57 2023E
CM014317.1_116368470 A03 116,368,470 8.56E-07 4.78 2023E
CM014317.1_116433136 A03 116,433,136 1.30E-07 5.62 2024E
CM014317.1_116677137 A03 116,677,137 7.01E-07 4.95 2024E
CM014317.1_116698458 A03 116,698,458 9.42E-07 3.52 2022L
CM014317.1_116746762 A03 116,746,762 6.20E-07 5.00 2024E
CM014317.1_116902961 A03 116,902,961 6.48E-08 5.81 2023E
CM014317.1_116902961 A03 116,902,961 4.11E-07 5.16 2024E
CM014317.1_116902961 A03 116,902,961 6.32E-07 3.54 BLUE
CM014317.1_116958729 A03 116,958,729 2.37E-08 4.49 BLUE
CM014317.1_116958729 A03 116,958,729 8.52E-08 5.80 2024E
CM014317.1_116958729 A03 116,958,729 2.12E-07 5.34 2023E
CM014317.1_116958729 A03 116,958,729 3.99E-07 3.77 2022L
CM014317.1_116973342 A03 116,973,342 4.38E-07 5.13 2024E
CM014317.1_116995415 A03 116,995,415 1.91E-07 5.38 2023E
CM014317.1_116995415 A03 116,995,415 3.54E-07 3.71 BLUE
CM014317.1_120553446 A03 120,553,446 5.30E-07 5.06 2024E
CM014317.1_132572915 A03 132,572,915 2.82E-07 5.10 2022E
CM014317.1_132572915 A03 132,572,915 4.96E-07 3.61 BLUE
CM014317.1_135969985 A03 135,969,985 7.41E-07 4.92 2024E
CM014317.1_136427337 A03 136,427,337 6.65E-07 4.97 2024E
CM014317.1_136427352 A03 136,427,352 9.93E-07 4.81 2024E
CM014317.1_137988661 A03 137,988,661 4.66E-07 4.91 2022E
CM014317.1_138005049 A03 138,005,049 3.39E-07 5.03 2022E
CM014317.1_139311738 A03 139,311,738 8.19E-07 4.69 2022E
CM014317.1_139435545 A03 139,435,545 3.19E-07 5.05 2022E
CM014317.1_143798496 A03 143,798,496 8.84E-07 4.85 2024E
CM014317.1_143798499 A03 143,798,499 4.60E-07 5.12 2024E
CM014317.1_143798500 A03 143,798,500 8.84E-07 4.85 2024E
CM014318.1_15983124 A04 15,983,124 4.91E-09 6.97 2024E
CM014318.1_15983124 A04 15,983,124 1.83E-08 4.56 BLUE
CM014318.1_22920839 A04 22,920,839 2.75E-07 3.88 2022L
CM014318.1_29241963 A04 29,241,963 8.87E-08 4.10 BLUE
CM014318.1_30049881 A04 30,049,881 1.15E-08 4.70 BLUE
CM014318.1_30049881 A04 30,049,881 8.24E-07 3.56 2022L
CM014318.1_31632349 A04 31,632,349 1.70E-08 4.58 BLUE
CM014318.1_31632349 A04 31,632,349 2.52E-07 3.91 2022L
CM014318.1_32105236 A04 32,105,236 7.07E-07 3.51 BLUE
CM014318.1_32530480 A04 32,530,480 1.11E-07 4.04 BLUE
CM014318.1_33970825 A04 33,970,825 3.35E-07 3.72 BLUE
CM014318.1_35411879 A04 35,411,879 9.02E-07 3.44 BLUE
CM014318.1_36370000 A04 36,370,000 5.30E-08 4.37 2022L
CM014318.1_39116486 A04 39,116,486 1.06E-07 4.05 BLUE
CM014318.1_43533623 A04 43,533,623 9.76E-07 3.42 BLUE
CM014318.1_43902747 A04 43,902,747 1.11E-07 4.04 BLUE
CM014318.1_44606845 A04 44,606,845 8.41E-07 3.46 BLUE
CM014318.1_49906055 A04 49,906,055 6.73E-07 3.52 BLUE
CM014318.1_55384280 A04 55,384,280 9.89E-08 4.07 BLUE
CM014318.1_55384280 A04 55,384,280 7.51E-07 4.72 2022E
CM014318.1_55723519 A04 55,723,519 2.95E-07 5.08 2022E
CM014318.1_61927441 A04 61,927,441 3.83E-07 3.68 BLUE
CM014318.1_66463994 A04 66,463,994 2.27E-07 3.83 BLUE
CM014318.1_66893612 A04 66,893,612 6.95E-07 4.95 2024E
CM014318.1_66893633 A04 66,893,633 6.95E-07 4.95 2024E
CM014318.1_71386694 A04 71,386,694 6.27E-07 3.54 BLUE
CM014318.1_71891139 A04 71,891,139 4.04E-07 3.67 BLUE
CM014318.1_72543660 A04 72,543,660 9.74E-07 3.42 BLUE
CM014318.1_89308224 A04 89,308,224 7.27E-08 4.16 BLUE
CM014318.1_99014233 A04 99,014,233 8.27E-08 4.12 BLUE
CM014319.1_48862897 A05 48,862,897 8.60E-09 4.78 BLUE
CM014319.1_48862897 A05 48,862,897 7.36E-07 4.93 2024E
CM014319.1_62731176 A05 62,731,176 7.85E-08 5.83 2024E
CM014319.1_95775271 A05 95,775,271 2.46E-07 5.16 2022E
CM014319.1_95775273 A05 95,775,273 2.46E-07 5.16 2022E
CM014320.1_9148065 A06 9,148,065 8.25E-07 4.69 2022E
CM014320.1_15439347 A06 15,439,347 1.60E-08 4.60 BLUE
CM014320.1_23458916 A06 23,458,916 6.87E-10 7.49 2022E
CM014320.1_84254355 A06 84,254,355 7.46E-07 4.92 2024E
CM014320.1_108762269 A06 108,762,269 8.60E-07 4.86 2024E
CM014321.1_24643355 A07 24,643,355 5.43E-07 3.68 2022L
CM014321.1_25901024 A07 25,901,024 3.75E-07 3.79 2022L
CM014321.1_93908115 A07 93,908,115 4.65E-11 8.58 2022E
CM014321.1_93908115 A07 93,908,115 1.77E-07 3.90 BLUE
CM014321.1_101512868 A07 101,512,868 3.43E-07 5.37 2023L
CM014321.1_102881662 A07 102,881,662 6.35E-08 4.31 2022L
CM014321.1_118561175 A07 118,561,175 9.00E-10 7.67 2024E
CM014321.1_167523336 A07 167,523,336 5.42E-07 3.59 BLUE
CM014322.1_20663269 A08 20,663,269 6.13E-07 4.80 2022E
CM014322.1_40029491 A08 40,029,491 3.54E-07 3.81 2022L
CM014322.1_43498908 A08 43,498,908 3.05E-08 6.22 2024E
CM014322.1_47929518 A08 47,929,518 2.90E-07 3.76 BLUE
CM014323.1_69917730 A09 69,917,730 5.42E-07 3.59 BLUE
CM014323.1_69917731 A09 69,917,731 5.42E-07 3.59 BLUE
CM014324.1_37983337 A10 37,983,337 8.48E-07 4.87 2024E
CM014324.1_38025192 A10 38,025,192 2.32E-07 3.93 2022L
CM014324.1_38025192 A10 38,025,192 7.96E-07 3.48 BLUE
CM014325.1_4855128 B01 4,855,128 2.56E-08 4.58 2022L
CM014325.1_4855128 B01 4,855,128 5.35E-08 4.25 BLUE
CM014325.1_4855130 B01 4,855,130 2.56E-08 4.58 2022L
CM014325.1_4855130 B01 4,855,130 5.35E-08 4.25 BLUE
CM014325.1_4855132 B01 4,855,132 2.56E-08 4.58 2022L
CM014325.1_4855132 B01 4,855,132 5.35E-08 4.25 BLUE
CM014325.1_75515583 B01 75,515,583 5.97E-08 5.71 2022E
CM014325.1_109012607 B01 109,012,607 4.38E-07 3.65 BLUE
CM014325.1_113814596 B01 113,814,596 4.15E-07 3.66 BLUE
CM014325.1_124640445 B01 124,640,445 6.27E-07 3.54 BLUE
CM014325.1_126303590 B01 126,303,590 6.58E-08 5.90 2024E
CM014325.1_128314353 B01 128,314,353 1.89E-09 7.36 2024E
CM014325.1_137352274 B01 137,352,274 2.01E-07 3.87 BLUE
CM014325.1_150607315 B01 150,607,315 2.55E-07 5.26 2023E
CM014326.1_24582876 B02 24,582,876 2.73E-08 4.44 BLUE
CM014326.1_63951503 B02 63,951,503 4.28E-07 5.14 2024E
CM014326.1_101904604 B02 101,904,604 1.58E-07 4.04 2022L
CM014326.1_101904604 B02 101,904,604 5.95E-07 3.56 BLUE
CM014326.1_108963274 B02 108,963,274 8.10E-07 4.70 2022E
CM014327.1_32555445 B03 32,555,445 4.06E-07 3.67 BLUE
CM014327.1_32555445 B03 32,555,445 4.98E-07 5.00 2023E
CM014327.1_88895359 B03 88,895,359 5.43E-07 3.58 BLUE
CM014327.1_102785556 B03 102,785,556 4.14E-07 5.16 2024E
CM014327.1_108688509 B03 108,688,509 4.71E-07 3.62 BLUE
CM014327.1_121762471 B03 121,762,471 9.31E-07 4.64 2022E
CM014327.1_135818949 B03 135,818,949 4.56E-07 4.92 2022E
CM014327.1_135818953 B03 135,818,953 4.56E-07 4.92 2022E
CM014327.1_135818957 B03 135,818,957 9.95E-07 4.62 2022E
CM014327.1_139869695 B03 139,869,695 1.91E-07 5.25 2022E
CM014327.1_140294263 B03 140,294,263 4.95E-07 3.61 BLUE
CM014328.1_2936773 B04 2,936,773 9.11E-07 4.84 2024E
CM014328.1_7440919 B04 7,440,919 5.77E-07 4.94 2023E
CM014328.1_20364742 B04 20,364,742 2.26E-07 3.83 BLUE
CM014328.1_65600064 B04 65,600,064 5.11E-08 6.00 2024E
CM014328.1_115422174 B04 115,422,174 7.69E-12 6.88 BLUE
CM014328.1_115422174 B04 115,422,174 6.52E-09 4.99 2022L
CM014328.1_115422174 B04 115,422,174 5.37E-08 5.88 2023E
CM014328.1_115422174 B04 115,422,174 1.34E-07 5.61 2024E
CM014328.1_124435515 B04 124,435,515 6.41E-08 4.20 BLUE
CM014329.1_98604 B05 98,604 2.21E-07 3.94 2022L
CM014329.1_18845024 B05 18,845,024 4.07E-07 4.96 2022E
CM014329.1_37463825 B05 37,463,825 7.43E-07 4.92 2024E
CM014329.1_37463863 B05 37,463,863 2.34E-07 5.39 2024E
CM014329.1_37463876 B05 37,463,876 2.18E-08 6.35 2024E
CM014329.1_79069836 B05 79,069,836 7.68E-07 3.58 2022L
CM014329.1_92820000 B05 92,820,000 9.19E-07 3.53 2022L
CM014329.1_97667993 B05 97,667,993 3.73E-07 3.79 2022L
CM014330.1_27293946 B06 27,293,946 6.78E-07 3.62 2022L
CM014330.1_42024591 B06 42,024,591 2.16E-08 4.51 BLUE
CM014330.1_42024591 B06 42,024,591 7.01E-08 5.88 2024E
CM014330.1_42024591 B06 42,024,591 7.78E-08 4.25 2022L
CM014330.1_42480868 B06 42,480,868 1.31E-07 4.10 2022L
CM014330.1_42706698 B06 42,706,698 3.25E-07 5.25 2024E
CM014330.1_68578992 B06 68,578,992 4.53E-07 3.64 BLUE
CM014330.1_133050189 B06 133,050,189 1.47E-07 3.96 BLUE
CM014330.1_133050189 B06 133,050,189 1.88E-07 3.9 2022L
CM014331.1_43345246 B07 43,345,246 2.72E-07 5.33 2024E
CM014331.1_55611630 B07 55,611,630 2.32E-08 4.49 BLUE
CM014331.1_55611630 B07 55,611,630 7.19E-08 5.87 2024E
CM014331.1_55743596 B07 55,743,596 1.75E-07 5.50 2024E
CM014331.1_55743596 B07 55,743,596 5.58E-07 3.58 BLUE
CM014332.1_2825768 B08 2,825,768 5.80E-07 5.02 2024E
CM014332.1_16036043 B08 16,036,043 5.72E-10 7.56 2022E
CM014332.1_56281078 B08 56,281,078 8.15E-07 3.47 BLUE
CM014332.1_82284008 B08 82,284,008 4.19E-08 6.08 2024E
CM014332.1_83218971 B08 83,218,971 4.56E-08 6.05 2024E
CM014332.1_94261894 B08 94,261,894 2.43E-08 6.31 2024E
CM014332.1_94261901 B08 94,261,901 2.14E-08 6.36 2024E
CM014332.1_122400731 B08 12,200,731 1.51E-08 6.25 2022E
CM014333.1_18572043 B09 18,572,043 5.58E-07 5.04 2024E
CM014333.1_18572047 B09 18,572,047 3.05E-08 6.22 2024E
CM014333.1_26746614 B09 26,746,614 2.84E-07 3.77 BLUE
CM014333.1_26746614 B09 26,746,614 6.70E-07 4.77 2022E
CM014333.1_28757101 B09 28,757,101 1.87E-07 3.99 2022L
CM014333.1_143530285 B09 143,530,285 4.14E-08 6.09 2024E
CM014333.1_143530285 B09 143,530,285 8.21E-07 3.47 BLUE
CM014333.1_161560858 B09 161,560,858 2.62E-07 5.13 2022E
CM014334.1_71318897 B10 71,318,897 3.61E-07 3.70 BLUE
CM014334.1_130789491 B10 130,789,491 8.82E-07 3.54 2022L
CM014334.1_130789492 B10 130,789,492 8.92E-07 3.54 2022L
CM014334.1_130789494 B10 130,789,494 3.48E-07 3.81 2022L
CM014334.1_132266202 B10 132,266,202 8.48E-07 4.87 2024E
CM014334.1_138883330 B10 138,883,330 8.60E-07 4.86 2024E

Table 2

Significant SNPs identified in more than two environments associated with flowering time in peanut"

标记
SNP		 染色体
Chromosome		 位置
Position		 P值
P-value		 表型变异贡献率
Phenotype variation explained (%)		 环境
Environment
CM014315.1_77174051 A01 77,174,051 6.13E-07-9.43E-07 3.64-4.83 2022L, 2024E
CM014315.1_105876877 A01 105,876,877 1.63E-07-6.94E-07 4.03-4.87 2022L, 2023E
CM014316.1_6463338 A02 6,463,338 1.67E-07-6.24E-07 3.92-4.99 2024E, BLUE
CM014316.1_37973138 A02 37,973,138 6.02E-11-8.30E-07 4.88-6.26 2024E, BLUE
CM014317.1_113914790 A03 113,914,790 5.58E-07-9.11-09 4.76-5.04 2024E, BLUE
CM014317.1_115657511 A03 115,657,511 1.05E-07-7.57E-09 4.16-7.63 2022E, 2022L, 2023E, 2024E, BLUE
CM014317.1_116902961 A03 116,902,961 4.11E-07-6.48E-08 3.54-5.81 2023E, 2024E, BLUE
CM014317.1_116958729 A03 116,958,729 2.12E-07-8.52E-08 3.77-5.80 2022L, 2023E, 2024E, BLUE
CM014317.1_116995415 A03 116,995,415 1.91E-07-3.54E-07 3.71-5.38 2023E, BLUE
CM014317.1_132572915 A03 132,572,915 2.82E-07-4.96E-07 3.61-5.10 2022E, BLUE
CM014318.1_15983124 A04 15,983,124 1.83E-08-4.91E-09 4.56-6.97 2024E, BLUE
CM014318.1_30049881 A04 30,049,881 1.15E-08-8.24E-07 3.56-4.70 2022L, BLUE
CM014318.1_31632349 A04 31,632,349 1.70E-08-2.52E-07 3.91-4.58 2022L, BLUE
CM014318.1_55384280 A04 55,384,280 7.51E-07-9.89E-08 4.07-4.72 2022E, BLUE
CM014319.1_48862897 A05 48,862,897 8.60E-09-7.36E-07 4.78-4.93 2024E, BLUE
CM014321.1_93908115 A07 93,908,115 1.77E-07-4.65E-11 3.90-8.58 2022E, BLUE
CM014324.1_38025192 A10 38,025,192 2.32E-07-7.96E-07 3.48-3.93 2022L, BLUE
CM014325.1_4855128 B01 4,855,128 2.56E-08-5.35E-08 4.25-4.58 2022L, BLUE
CM014325.1_4855130 B01 4,855,130 2.56E-08-5.35E-08 4.25-4.58 2022L, BLUE
CM014325.1_4855132 B01 4,855,132 2.56E-08-5.35E-08 4.25-4.58 2022L, BLUE
CM014326.1_101904604 B02 101,904,604 1.58E-07-5.95E-07 3.56-4.04 2022L, BLUE
CM014327.1_32555445 B03 32,555,445 4.06E-07-4.98E-07 3.67-5.00 2023E, BLUE
CM014328.1_115422174 B04 115,422,174 1.34E-07-7.69E-12 4.99-6.88 2022L, 2023E, 2024E, BLUE
CM014330.1_42024591 B06 42,024,591 2.16E-08-7.78E-08 4.25-5.88 2022L, 2024E, BLUE
CM014330.1_133050189 B06 133,050,189 1.47E-07-1.88E-07 3.96-3.99 2022L, BLUE
CM014331.1_55611630 B07 55,611,630 2.32E-08-7.19E-08 4.49-5.87 2024E, BLUE
CM014331.1_55743596 B07 55,743,596 1.75E-07-5.58E-07 3.58-5.50 2024E, BLUE
CM014333.1_26746614 B09 26,746,614 2.84E-07-6.70E-07 3.77-4.77 2022E, BLUE
CM014333.1_143530285 B09 143,530,285 4.14E-08-8.21E-07 3.47-6.09 2024E, BLUE

Table S2

List of candidate genes"

染色体
Chromosome		 位置
Position		 基因ID
Gene ID		 物理位置
Physical position		 功能注释
Function annotation
A01 77174051 Ahy_A01g003024 77134385-77136294 未知蛋白Uncharacterized protein
Ahy_A01g003025 77160337-77160690 RNA结合蛋白42 RNA-binding protein 42
Ahy_A01g003026 77231350-77235272 酪蛋白激酶II Casein kinase II
A01 105876877 Ahy_A01g004429 105847145-105851326 未知蛋白 Uncharacterized protein
Ahy_A01g004431 105870727-105873302 未知蛋白 Uncharacterized protein
Ahy_A01g004433 105921375-105922649 C2H2型结构域蛋白
C2H2-type domain-containing protein
Ahy_A01g004434 105948778-105954179 阴离子转运蛋白(叶绿体)
Anion transporter chloroplast-like
Ahy_A01g004435 105954652-105956736 细胞色素P450 cytochrome P450
Ahy_A01g004436 105958899-105959471 未知蛋白 Uncharacterized protein
Ahy_A01g004439 105969631-105969912 未知蛋白 Uncharacterized protein
Ahy_A01g004440 105971042-105978618 磷酸甲基嘧啶合成酶
Phosphomethylpyrimidine synthase
A02 6463338 Ahy_A02g005513 6367310-6369682 50S核糖体蛋白L18
50S ribosomal protein L18
Ahy_A02g005514 6368408-6368896 酰基磷酸酶Acylphosphatase
Ahy_A02g005515 6484048-6486976 R2R3-MYB转录因子
R2R3-MYB transcription factor, putative
Ahy_A02g005516 6490810-6500697 AMPK1_CBM结构域蛋白
AMPK1_CBM domain-containing protein
Ahy_A02g005517 6495947-6503017 未知蛋白Uncharacterized protein
Ahy_A02g005518 6503204-6508438 Tau95_N结构域蛋白
Tau95_N domain-containing protein
Ahy_A02g005519 6514126-6518808 木葡聚糖内转葡萄糖基化酶
Xyloglucan endotransglucosylase
Ahy_A02g005521 6528866-6531320 60S核糖体蛋白L7-4
60S ribosomal protein L7-4
Ahy_A02g005522 6531632-6532342 未知蛋白Uncharacterized protein
Ahy_A02g005523 6539814-6542381 未知蛋白Uncharacterized protein
Ahy_A02g005524 6557301-6559669 未知蛋白Uncharacterized protein
A02 37973138 - - -
A03 113914790 Ahy_A03g014642 113869444-113872841 金属硫蛋白Metallothionein-like protein
Ahy_A03g014643 113873521-113874677 未知蛋白Uncharacterized protein
Ahy_A03g014644 113874681-113875001 未知蛋白Uncharacterized protein
Ahy_A03g014647 113879583-113882637 未知蛋白Uncharacterized protein
Ahy_A03g014648 113907655-113908095 RING-H2指纹蛋白ATL3G
RING-H2 finger protein ATL3G
Ahy_A03g014649 113913722-113914458 未知蛋白Uncharacterized protein
Ahy_A03g014650 113919892-113920062 未知蛋白Uncharacterized protein
Ahy_A03g014652 113964557-113971341 未知蛋白Uncharacterized protein
Ahy_A03g014654 113976981-113981237 未知蛋白Uncharacterized protein
A03 115657511 Ahy_A03g014771 115570029-115570648 未知蛋白Uncharacterized protein
Ahy_A03g014772 115571993-115577697 甘露糖基转移酶Mannosyl transferase
Ahy_A03g014773 115592818-115597445 泛素结合酶E2
Ubiquitin-conjugating enzyme E2
Ahy_A03g014774 115598472-115600356 未知蛋白Uncharacterized protein
Ahy_A03g014776 115630492-115636706 微管蛋白γ链Tubulin gamma chain
Ahy_A03g014777 115637605-115637959 阿拉伯半乳聚糖肽20
Arabinogalactan peptide 20
Ahy_A03g014778 115638498-115642037 线粒体转运蛋白Mitochondrial carrier protein
Ahy_A03g014782 115713075-115715113 含PMD结构域蛋白
PMD domain-containing protein
Ahy_A03g014783 115719741-115721966 未知蛋白Uncharacterized protein
Ahy_A03g014784 115747021-115747825 磷脂二酰基甘油酰基转移酶
Phospholipid: diacylglycerol acyltransferase
Ahy_A03g014785 115754216-115757473 TRNA (腺嘌呤(58)-N(1))-甲基转移酶
tRNA (adenine(58)-N(1))-methyltransferase
A03 116902961 Ahy_A03g014837 116863472-116872086 甲硫氨酸γ -裂解酶
Methionine gamma-lyase
Ahy_A03g014838 116865911-116872106 未知蛋白Uncharacterized protein
Ahy_A03g014841 116919745-116925270 PCRF结构域蛋白
PCRF domain-containing protein
Ahy_A03g014843 116977120-116988871 未知蛋白Uncharacterized protein
Ahy_A03g014844 116989329-116990737 叶绿素a-b结合蛋白，叶绿体
Chlorophyll a-b binding protein, chloroplasts
Ahy_A03g014845 116994526-117000306 含五肽重复序列蛋白
Pentatricopeptide repeat-containing protein
A03 116958729 Ahy_A03g014837 116863472-116872086 甲硫氨酸γ -裂解酶Methionine gamma-lyase
Ahy_A03g014838 116865911-116872106 未知蛋白Uncharacterized protein
Ahy_A03g014841 116919745-116925270 PCRF结构域蛋白
PCRF domain-containing protein
Ahy_A03g014843 116977120-116988871 未知蛋白Uncharacterized protein
Ahy_A03g014844 116989329-116990737 叶绿素a-b结合蛋白，叶绿体
Chlorophyll a-b binding protein, chloroplasts
Ahy_A03g014845 116994526-117000306 含五肽重复序列蛋白
Pentatricopeptide repeat-containing protein
Ahy_A03g014846 117003718-117008306 丝氨酸结合蛋白Serine incorporator-like protein
Ahy_A03g014848 117011361-117015232 未知蛋白Uncharacterized protein
A03 116995415 Ahy_A03g014841 116919745-116925270 PCRF结构域蛋白 PCRF domain-containing protein
Ahy_A03g014843 116977120-116988871 未知蛋白Uncharacterized protein
Ahy_A03g014844 116989329-116990737 叶绿素a-b结合蛋白，叶绿体
Chlorophyll a-b binding protein, chloroplasts
Ahy_A03g014845 116994526-117000306 含五肽重复序列蛋白
Pentatricopeptide repeat-containing protein
Ahy_A03g014846 117003718-117008306 丝氨酸结合蛋白 Serine incorporator-like protein
Ahy_A03g014848 117011361-117015232 未知蛋白Uncharacterized protein
Ahy_A03g014849 117092538-11710553 环核苷酸结合结构域的蛋白质
Cyclic nucleotide-binding domain-containing protein
A03 132572915 Ahy_A03g015793 132501545-132504127 环型E3泛素转移酶 E3 ubiquitin transferase
Ahy_A03g015794 132513856-132517728 生长抑制与分化相关蛋白88
Growth inhibition and differentiation-related protein 88 like
Ahy_A03g015795 132531855-132538862 类BEL1样同源结构域蛋白1
BEL1-like homeodomain protein 1
Ahy_A03g015796 132545380-132546803 未知蛋白Uncharacterized protein
Ahy_A03g015797 132583193-132583459 未知蛋白Uncharacterized protein (Fragment)
Ahy_A03g015798 132623590-132627304 未知蛋白Uncharacterized protein
Ahy_A03g015800 132626077-132628580 未知蛋白Uncharacterized protein
Ahy_A03g015801 132640100-132646618 BHLH结构域蛋白 BHLH domain-containing protein
A04 15983124 Ahy_A04g018484 15927838-15930633 未知蛋白Uncharacterized protein
Ahy_A04g018485 15961401-15964430 PALP结构域的蛋白
PALP domain-containing protein
Ahy_A04g018489 16081091-16085043 CRS2相关因子1，线粒体
CRS2-associated factor 1, mitochondrial
A04 30049881 Ahy_A04g019172 29991643-29993015 VARLMGL结构域蛋白
VARLMGL domain-containing protein
Ahy_A04g019174 30009022-30009339 未知蛋白Uncharacterized protein
Ahy_A04g019175 30017220-30020790 未知蛋白Uncharacterized protein
A04 31632349 Ahy_A04g019249 31582475-31583693 未知蛋白Uncharacterized protein
Ahy_A04g019251 31589216-31611319 ADH_N结构域蛋白
ADH_N domain-containing protein
A04 55384280 - - -
A05 48862897 Ahy_A05g024176 48843869-48859339 ANK_REP_REGION结构域蛋白
ANK_REP_REGION domain-containing protein
A07 93908115 Ahy_A07g035384 93811001-93832360 糖转运蛋白5 Sugar transport protein 5
Ahy_A07g035385 93833288-93839228 MAG2相互作用蛋白2 MAG2-interacting protein 2
Ahy_A07g035387 93840118-93841440 突变子亚类蛋白 Putative mutator sub-class protein
Ahy_A07g035392 93880370-93884378 膜联蛋白 Annexin
A10 38025192 Ahy_A10g048791 37954026-37956664 WRKY转录因子 WRKY family transcription factor
Ahy_A10g048792 38025580-38039757 未知蛋白Uncharacterized protein
Ahy_A10g048793 38049378-38050313 未知蛋白Uncharacterized protein
Ahy_A10g048794 38061626-38067893 核仁蛋白12 Nucleolar protein 12
B01 4855128 Ahy_B01g051777 4762080-4764082 未知蛋白Uncharacterized protein
Ahy_B01g051778 4789537-4792103 未知蛋白Uncharacterized protein
Ahy_B01g051779 4813689-4818706 天冬酰胺合成酶Asparagine synthetase
Ahy_B01g051780 4818005-4825082 酰基酯酶蛋白Acyl-esterase family protein
Ahy_B01g051782 4856691-4864675 未知蛋白Uncharacterized protein
Ahy_B01g051784 4916920-4918811 未知蛋白Uncharacterized protein
Ahy_B01g051786 4926241-4928598 蛋白FAR1相关序列 Protein FAR1-related sequence
Ahy_B01g051787 4940902-4941513 未知蛋白Uncharacterized protein
B01 4855130 Ahy_B01g051777 4762080-4764082 未知蛋白Uncharacterized protein
Ahy_B01g051778 4789537-4792103 未知蛋白Uncharacterized protein
Ahy_B01g051779 4813689-4818706 天冬酰胺合成酶Asparagine synthetase
Ahy_B01g051780 4818005-4825082 酰基酯酶蛋白Acyl-esterase family protein
Ahy_B01g051782 4856691-4864675 未知蛋白Uncharacterized protein
Ahy_B01g051784 4916920-4918811 未知蛋白Uncharacterized protein
Ahy_B01g051786 4926241-4928598 蛋白FAR1相关序列 Protein FAR1-related sequence
Ahy_B01g051787 4940902-4941513 未知蛋白Uncharacterized protein
B01 4855132 Ahy_B01g051777 4762080-4764082 未知蛋白Uncharacterized protein
Ahy_B01g051778 4789537-4792103 未知蛋白Uncharacterized protein
Ahy_B01g051779 4813689-4818706 天冬酰胺合成酶Asparagine synthetase
Ahy_B01g051780 4818005-4825082 酰基酯酶蛋白Acyl-esterase family protein
Ahy_B01g051782 4856691-4864675 未知蛋白Uncharacterized protein
Ahy_B01g051784 4916920-4918811 未知蛋白Uncharacterized protein
Ahy_B01g051786 4926241-4928598 蛋白FAR1相关序列 Protein FAR1-related sequence
Ahy_B01g051787 4940902-4941513 未知蛋白Uncharacterized protein
B02 101904604 Ahy_B02g060932 101810192-101817041 尿酸酶Uricase
Ahy_B02g060933 101834228-101840119 CCHC型结构域蛋白
CCHC-type domain-containing protein
Ahy_B02g060934 101846821-101852135 漆酶Laccase
Ahy_B02g060936 101873704-101874608 未知蛋白Uncharacterized protein
Ahy_B02g060937 101886617-101888644 未知蛋白Uncharacterized protein
Ahy_B02g060938 101892620-101893819 未知蛋白Uncharacterized protein
Ahy_B02g060939 101913258-101915279 未知蛋白Uncharacterized protein
Ahy_B02g060941 101935208-101937211 未知蛋白Uncharacterized protein
Ahy_B02g060942 101939427-101941676 未知蛋白Uncharacterized protein
Ahy_B02g060943 101947923-101951442 未知蛋白Uncharacterized protein
Ahy_B02g060944 101953900-101958277 未知蛋白Uncharacterized protein
Ahy_B02g060945 101987689-101992529 未知蛋白Uncharacterized protein
Ahy_B02g060946 101994931-101997085 动力蛋白相关蛋白Dynamin-related protein
Ahy_B02g060947 101997631-102014053 碳氮水解酶Carbon-nitrogen hydrolase
B03 32555445 Ahy_B03g064109 32628207-32641720 未知蛋白Uncharacterized protein
B04 115422174 Ahy_B04g073080 115365992-115372317 未知蛋白Uncharacterized protein
Ahy_B04g073081 115372718-115380673 未知蛋白Uncharacterized protein
B06 42024591 Ahy_B06g082336 41957160-41959443 V型质子ATP酶亚基G
V-type proton ATPase subunit G
Ahy_B06g082337 41973993-41980423 未知蛋白Uncharacterized protein
Ahy_B06g082339 42035853-42039576 未知蛋白Uncharacterized protein
B06 133050189 Ahy_B06g084736 132951068-132952115 未知蛋白Uncharacterized protein
Ahy_B06g084737 132969873-132975051 过氧化物酶Peroxidase
Ahy_B06g084739 133000879-133001293 未知蛋白Uncharacterized protein
Ahy_B06g084740 133001321-133002714 未知蛋白Uncharacterized protein
Ahy_B06g084741 133002751-133003266 未知蛋白Uncharacterized protein
Ahy_B06g084744 133068551-133071773 囊泡亚基Exo70家族蛋白
Exocyst subunit Exo70 family protein
Ahy_B06g084745 133086657-133089784 丝氨酸/苏氨酸蛋白磷酸酶7
Serine/threonine protein phosphatase 7
Ahy_B06g084746 133088050-133092794 未知蛋白Uncharacterized protein
Ahy_B06g084748 133103406-133108116 未知蛋白Uncharacterized protein
B07 55611630 Ahy_B07g088873 55519735-55521528 未知蛋白Uncharacterized protein
Ahy_B07g088874 55547058-55553374 未知蛋白Uncharacterized protein
Ahy_B07g088875 55554197-55557525 Cation_ATPase_C结构域蛋白
Cation_ATPase_C domain-containing protein
Ahy_B07g088876 55558199-55559793 叶绿素a-b结合蛋白，叶绿体
Chlorophyll a-b binding protein, chloroplasts
Ahy_B07g088877 55560198-55562243 DYW结构域蛋白质
DYW domain-containing protein
Ahy_B07g088878 55578651-55579289 未知蛋白Uncharacterized protein
Ahy_B07g088879 55579302-55579700 未知蛋白Uncharacterized protein
Ahy_B07g088880 55582036-55582734 CBS结构域蛋白CBSX2，叶绿体
CBS domain-containing protein CBSX2, chloroplasts
Ahy_B07g088881 55594796-55600126 热休克蛋白90 Heat shock protein 90
Ahy_B07g088882 55600373-55603426 OBERON蛋白OBERON-like protein
Ahy_B07g088883 55603524-55604108 未知蛋白Uncharacterized protein
Ahy_B07g088884 55615148-55624599 组氨酸激酶2 Histidine kinase 2
Ahy_B07g088885 55624793-55626122 葡聚糖内切-1,3-葡糖苷酶
Glucan endo-1,3-beta-glucosidase 4
Ahy_B07g088886 55625164-55627989 未知蛋白Uncharacterized protein
Ahy_B07g088887 55631074-55653097 未知蛋白Uncharacterized protein
Ahy_B07g088888 55632512-55635337 Nop结构域蛋白 Nop domain-containing protein
Ahy_B07g088889 55637843-55641593 Nop结构域蛋白 Nop domain-containing protein
Ahy_B07g088890 55643192-55646346 酰胺酶Amidase
Ahy_B07g088891 55648069-55650062 Nop结构域蛋白 Nop domain-containing protein
Ahy_B07g088892 55653983-55656647 含五肽重复序列蛋白
Pentatricopeptide repeat-containing protein
Ahy_B07g088893 55669000-55670592 GRAS结构域的蛋白
GRAS domain-containing protein
Ahy_B07g088894 55671262-55678427 WRKY转录因子1 WRKY transcription factor 1
Ahy_B07g088895 55676724-55678271 未知蛋白Uncharacterized protein
Ahy_B07g088896 55681723-55702602 DAZ相关蛋白1 DAZ-associated protein 1
Ahy_B07g088897 55704012-55705208 细胞色素b561 Cytochrome b561
Ahy_B07g088898 55706014-55709031 未知蛋白Uncharacterized protein
Ahy_B07g088899 55709778-55713565 类MO25-like蛋白 MO25-like protein
B07 55743596 Ahy_B07g088891 55648069-55650062 Nop结构域蛋白 Nop domain-containing protein
Ahy_B07g088892 55653983-55656647 含五肽重复序列蛋白
Pentatricopeptide repeat-containing protein
Ahy_B07g088893 55669000-55670592 GRAS结构域的蛋白
GRAS domain-containing protein
Ahy_B07g088894 55671262-55678427 WRKY转录因子1 WRKY transcription factor 1
Ahy_B07g088895 55676724-55678271 未知蛋白Uncharacterized protein
Ahy_B07g088896 55681723-55702602 DAZ相关蛋白1 DAZ-associated protein 1
Ahy_B07g088897 55704012-55705208 细胞色素b561 Cytochrome b561
Ahy_B07g088898 55706014-55709031 未知蛋白Uncharacterized protein
Ahy_B07g088899 55709778-55713565 类MO25-like蛋白 MO25-like protein
Ahy_B07g088900 55714551-55715465 未知蛋白Uncharacterized protein
Ahy_B07g088901 55717284-55719201 未知蛋白Uncharacterized protein
Ahy_B07g088905 55729160-55738241 肽酶S59结构域蛋白
Peptidase S59 domain-containing protein
Ahy_B07g088906 55737284-55739497 Aldo_ket_red结构域蛋白
Aldo_ket_red domain-containing protein
Ahy_B07g088907 55740026-55741700 6'-脱氧查尔酮合成酶样蛋白
6'-deoxychalcone synthase-like protein
Ahy_B07g088908 55743723-55745620 6'-脱氧查尔酮合成酶样蛋白
6'-deoxychalcone synthase-like protein
Ahy_B07g088909 55750971-55752870 Aldo_ket_red结构域蛋白
Aldo_ket_red domain-containing protein
Ahy_B07g088910 55755723-55758158 未知蛋白Uncharacterized protein
Ahy_B07g088911 55774915-55788866 查尔酮还原酶Chalcone reductase
Ahy_B07g088913 55792600-55794860 Rho GDP -解离抑制剂1
Rho GDP-dissociation inhibitor 1
Ahy_B07g088914 55800789-55808174 丝束蛋白-5 Fimbrin-5
Ahy_B07g088915 55808899-55812881 TBC1结构域家族成员2A
TBC1 domain family member 2A
B09 26746614 Ahy_B09g096161 26654325-26659641 未知蛋白Uncharacterized protein
Ahy_B09g096162 26658143-26662853 肽基脯氨酸顺反异构酶
Peptidyl-prolyl cis-trans isomerase
Ahy_B09g096163 26693889-26698567 Polyketide_cyc结构域蛋白
Polyketide_cyc domain-containing protein
Ahy_B09g096164 26696435-26703831 Alpha-1,2-甘露糖基转移酶
Alpha-1,2-mannosyltransferase
Ahy_B09g096165 26758080-26760671 未知蛋白Uncharacterized protein
Ahy_B09g096167 26774983-26780065 未知蛋白Uncharacterized protein
Ahy_B09g096168 26781673-26784032 扩张蛋白Expansin
Ahy_B09g096169 26788451-26788748 未知蛋白Uncharacterized protein
Ahy_B09g096171 26801836-26802934 未知蛋白Uncharacterized protein
B09 143530285 Ahy_B09g098654 143467561-143468382 凝集素-1 Agglutinin-1

Table 3

Prediction of candidate genes for peanut flowering"

染色体
Chromosome		 基因ID
Gene ID		 物理位置
Physical position		 功能注释
Functional annotation
A02 Ahy_A02g005515 6,484,048-6,486,976 R2R3-MYB转录因子 R2R3-MYB transcription factor
A03
 Ahy_A03g014844
 116,989,329-116,990,737
 叶绿素a-b结合蛋白, 叶绿体
Chlorophyll a-b binding protein, chloroplasts
A03 Ahy_A03g015801 132,640,100-132,646,618 bHLH结构域蛋白 bHLH domain-containing protein
A10 Ahy_A10g048791 37,954,026-37,956,664 WRKY转录因子 WRKY family transcription factor
B01 Ahy_B01g051786 4,926,241-4,928,598 蛋白FAR1相关序列 Protein FAR1-related sequence
B07
 Ahy_B07g088876
 55,558,199-55,559,793
 叶绿素a-b结合蛋白, 叶绿体
Chlorophyll a-b binding protein, chloroplasts
B07 Ahy_B07g088894 55,671,262-55,678,427 WRKY转录因子1 WRKY transcription factor 1
[1] 刘珂珂, 郭峰, 杨莎, 王建国, 高华鑫, 邹洁, 张佳蕾, 万书波. “二次镇压”对花生子叶节的出土调控增产效果. 中国油料作物学报, 2025, 47: 160-166.
doi: 10.19802/j.issn.1007-9084.2023265
Liu K K, Guo F, Yang S, Wang J G, Gao H X, Zou J, Zhang J L, Wan S B. “Second suppression” on peanut cotyledon festival. Chin J Oil Crop Sci, 2025, 47: 160-166 (in Chinese with English abstract).
[2] 田永国, 胡廷会, 丁广刚, 成良强, 饶庆琳, 姜敏, 王金花, 吕建伟, 王军. 花生荚果发育和成熟阶段的秸秆品质变化分析. 花生学报, 2023, 52(2): 91-95.
Tian Y G, Hu T H, Ding G G, Cheng L Q, Rao Q L, Jiang M, Wang J H, Lyu J W, Wang J. Analysis on dynamics of peanut straw quality at pod developmental and harvesting stages. J Peanut Sci, 2023, 52(2): 91-95 (in Chinese with English abstract).
[3] Upadhyaya H D, Nigam S N. Inheritance of two components of early maturity in groundnut (Arachis hypogaea L.). Euphytica, 1994, 78: 59-67.
[4] Irish V F. The flowering of Arabidopsis flower development. Plant J, 2010, 61: 1014-1028.
[5] Lin X Y, Liu B H, Weller J L, Abe J, Kong F J. Molecular mechanisms for the photoperiodic regulation of flowering in soybean. J Integr Plant Biol, 2021, 63: 981-994.
doi: 10.1111/jipb.13021
[6] 曹永策, 李曙光, 张新草, 孔杰杰, 赵团结. 夏大豆重组自交系群体遗传图谱构建及开花期QTL分析. 中国农业科学, 2020, 53: 683-694.
doi: 10.3864/j.issn.0578-1752.2020.04.002
Cao Y C, Li S G, Zhang X C, Kong J J, Zhao T J. Construction of genetic map and mapping QTL for flowering time in A summer planting soybean recombinant inbred line population. Sci Agric Sin, 2020, 53: 683-694 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2020.04.002
[7] Shirasawa K, Koilkonda P, Aoki K, Hirakawa H, Tabata S, Watanabe M, Hasegawa M, Kiyoshima H, Suzuki S, Kuwata C, et al. In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut. BMC Plant Biol, 2012, 12: 80.
doi: 10.1186/1471-2229-12-80 pmid: 22672714
[8] 李华东, 杨会, 吕玉英, 张秀荣, 张昆, 刘风珍, 万勇善. 栽培花生花期相关QTL定位. 山东农业科学, 2021, 53(2): 1-6.
Li H D, Yang H, Lyu Y Y, Zhang X R, Zhang K, Liu F Z, Wan Y S. QTL mapping for flowering time in cultivated peanut (Arachis hypogaea L.). Shandong Agric Sci, 2021, 53(2): 1-6 (in Chinese with English abstract).
[9] Zhong C, Li Z, Cheng Y L, Zhang H N, Liu Y, Wang X G, Jiang C J, Zhao X H, Zhao S L, Wang J, et al. Comparative genomic and expression analysis insight into evolutionary characteristics of PEBP genes in cultivated peanuts and their roles in floral induction. Int J Mol Sci, 2022, 23: 12429.
[10] Wang L, Yang X L, Cui S L, Zhao N N, Li L, Hou M Y, Mu G J, Liu L F, Li Z C. High-density genetic map development and QTL mapping for concentration degree of floret flowering date in cultivated peanut (Arachis hypogaea L.). Mol Breed, 2020, 40: 17.
[11] 杨飞, 张征锋, 南波, 肖本泽. 水稻产量相关性状的全基因组关联分析及候选基因筛选. 作物学报, 2022, 48: 1813-1821.
doi: 10.3724/SP.J.1006.2022.12047
Yang F, Zhang Z F, Nan B, Xiao B Z. Genome-wide association analysis and candidate gene selection of yield related traits in rice. Acta Agron Sin, 2022, 48: 1813-1821 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2022.12047
[12] 彭小爱, 卢茂昂, 张玲, 刘童, 曹磊, 宋有洪, 郑文寅, 何贤芳, 朱玉磊. 基于55K SNP芯片的小麦籽粒主要品质性状的全基因组关联分析. 作物学报, 2024, 50: 1948-1960.
doi: 10.3724/SP.J.1006.2024.31052
Peng X A, Lu M A, Zhang L, Liu T, Cao L, Song Y H, Zheng W Y, He X F, Zhu Y L. Genome-wide association study of major grain quality traits in wheat based on 55K SNP arrays. Acta Agron Sin, 2024, 50: 1948-1960 (in Chinese with English abstract).
[13] 杨豪, 向仕华, 刘丽, 宁可君, 杨雪, 舒英杰, 何庆元. 川渝大豆生育期性状的全基因组关联分析. 作物学报, 2023, 49: 2727-2737.
Yang H, Xiang S H, Liu L, Ning K J, Yang X, Shu Y J, He Q Y. Genome-wide association analysis of growth period traits in soybean of Sichuan and Chongqing. Acta Agron Sin, 2023, 49: 2727-2737 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2023.24210
[14] Long Y, Shi J, Qiu D, Li R, Zhang C, Wang J, Hou J, Zhao J, Shi L, Park B S, et al. Flowering time quantitative trait Loci analysis of oilseed Brassica in multiple environments and genome wide alignment with Arabidopsis. Genetics, 2007, 177: 2433-2444.
doi: 10.1534/genetics.107.080705 pmid: 18073439
[15] 黄吉祥, 熊化鑫, 潘兵, 倪西源, 张晓玉, 赵坚义. 油菜开花期QTL定位及与粒重的遗传关联性. 中国农业科学, 2016, 49: 3073-3083.
doi: 10.3864/j.issn.0578-1752.2016.16.002
Huang J X, Xiong H X, Pan B, Ni X Y, Zhang X Y, Zhao J Y. Mapping QTL of flowering time and their genetic relationships with seed weight in Brassica napus. Sci Agric Sin, 2016, 49: 3073-3083 (in Chinese with English abstract).
[16] Chen X P, Lu Q, Liu H, Zhang J N, Hong Y B, Lan H F, Li H F, Wang J P, Liu H Y, Li S X, et al. Sequencing of cultivated peanut, Arachis hypogaea, yields insights into genome evolution and oil improvement. Mol Plant, 2019, 12: 920-934.
[17] Lu Q, Huang L, Liu H, Garg V, Gangurde S S, Li H F, Chitikineni A, Guo D D, Pandey M K, Li S X, et al. A genomic variation map provides insights into peanut diversity in China and associations with 28 agronomic traits. Nat Genet, 2024, 56: 530-540.
[18] 梁晓艳, 付娆, 李佳佳, 衣葵花, 唐朝辉, 郭峰, 王建国, 张佳蕾, 万书波. 不同密度单粒精播对花生开花动态及结实特性的影响. 中国油料作物学报, 2024, 46: 450-459.
doi: 10.19802/j.issn.1007-9084.2024047
Liang X Y, Fu R, Li J J, Yi K H, Tang C H, Guo F, Wang J G, Zhang J L, Wan S B. Effect of different densities of single-seed sowing on flowering dynamics and fruiting characteristics of peanut. Chin J Oil Crop Sci, 2024, 46: 450-459 (in Chinese with English abstract).
[19] 赵存花, 李俊庆, 张贵国. 旱地花生开花规律及有效花终止期的研究. 中国油料作物学报, 1998, 20(2): 52-54.
Zhao C H, Li J Q, Zhang G G. Studies on flowering habit and closing date for effective flower in dry-land peanut. Chin J Oil Crop Sci, 1998, 20: 52-54 (in Chinese with English abstract).
[20] Fonceka D, Tossim H A, Rivallan R, Vignes H, Faye I, Ndoye O, Moretzsohn M C, Bertioli D J, Glaszmann J C, Courtois B, et al. Fostered and left behind alleles in peanut: interspecific QTL mapping reveals footprints of domestication and useful natural variation for breeding. BMC Plant Biol, 2012, 12: 26.
doi: 10.1186/1471-2229-12-26 pmid: 22340522
[21] Guo D S, Zhang J Z, Wang X L, Han X, Wei B Y, Wang J Q, Li B X, Yu H, Huang Q P, Gu H Y, et al. The WRKY transcription factor WRKY71/EXB1 controls shoot branching by transcriptionally regulating RAX genes in Arabidopsis. Plant Cell, 2015, 27: 3112-3127.
[22] Yang F, Wang Q, Schmitz G, Müller D, Theres K. The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis. Plant J, 2012, 71: 61-70.
[23] 韩艺, 赵继发, 蔡璨, 彭疑芳, 张梅娟, 沙伟, 马天意. 光捕获叶绿素a/b结合蛋白基因在植物逆境胁迫中的研究进展. 高师理科学刊, 2023, 43(6): 79-83.
Han Y, Zhao J F, Cai C, Peng Y F, Zhang M J, Sha W, Ma T Y. Research progress of light-harvesting chlorophyll a/b-binding protein genes in plant stresses. J Sci Teach Coll Univ, 2023, 43(6): 79-83 (in Chinese with English abstract).
[24] Han X W, Han S, Li Y T, Li K K, Yang L J, Ma D F, Fang Z W, Yin J L, Zhu Y X, Gong S J. Double roles of light-harvesting chlorophyll a/b binding protein TaLhc2 in wheat stress tolerance and photosynthesis. Int J Biol Macromol, 2023, 253: 127215.
[25] Hao Y Q, Zong X M, Ren P, Qian Y Q, Fu A G. Basic helix-loop-helix (bHLH) transcription factors regulate a wide range of functions in Arabidopsis. Int J Mol Sci, 2021, 22: 7152.
[26] Ito S, Song Y H, Josephson-Day A R, Miller R J, Breton G, Olmstead R G, Imaizumi T. Flowering BHLH transcriptional activators control expression of the photoperiodic flowering regulator CONSTANS in Arabidopsis. Proc Natl Acad Sci USA, 2012, 109: 3582-3587.
[27] 赵小波, 李春娟, 孙全喜, 王奇, 牟艺菲, 王娟, 苑翠玲, 姜常松, 单世华. 基于全基因组水平的花生FAR1转录因子家族分析. 花生学报, 2024, 53(2): 1-9.
Zhao X B, Li C J, Sun Q X, Wang Q, Mou Y F, Wang J, Yuan C L, Jiang C S, Shan S H. Genome-wide analysis of the FAR1 gene family in peanut. J Peanut Sci, 2024, 53(2): 1-9 (in Chinese with English abstract).
[28] Lu Q, Liu H, Hong Y B, Liang X Q, Li S X, Liu H Y, Li H F, Wang R F, Deng Q Q, Jiang H F, et al. Genome-wide identification and expression of FAR1 gene family provide insight into pod development in peanut (Arachis hypogaea). Front Plant Sci, 2022, 13: 893278.
[29] Ma L, Li G. FAR1-RELATED SEQUENCE (FRS) and FRS-RELATED FACTOR (FRF) family proteins in Arabidopsis growth and development. Front Plant Sci, 2018, 9: 692.
[30] Li G, Siddiqui H, Teng Y B, Lin R C, Wan X Y, Li J G, Lau O S, Ou-Yang X H, Dai M Q, Wan J M, et al. Coordinated transcriptional regulation underlying the circadian clock in Arabidopsis. Nat Cell Biol, 2011, 13: 616-622.
[31] Li D M, Fu X, Guo L, Huang Z G, Li Y P, Liu Y, He Z S, Cao X W, Ma X H, Zhao M C, et al. FAR-RED ELONGATED HYPOCOTYL3 activates SEPALLATA2 but inhibits CLAVATA3 to regulate meristem determinacy and maintenance in Arabidopsis. Proc Natl Acad Sci USA, 2016, 113: 9375-9380.
[32] 叶王斌, 王玲, 周晟, 杨娟, 刘桂伶, 范丽娟. 燕子花WRKY11基因克隆、结构分析及功能验证. 东北林业大学学报, 2023, 51(7): 1-9.
Ye W B, Wang L, Zhou S, Yang J, Liu G L, Fan L J. Cloning, structure analysis and functional verification of WRKY11 in iris laevigata. J Northeast For Univ, 2023, 51(7) 1-9 (in Chinese with English abstract).
[33] Zhang L P, Chen L G, Yu D Q. Transcription factor WRKY75 interacts with DELLA proteins to affect flowering. Plant Physiol, 2018, 176: 790-803.
[34] 张雁明, 邢国芳, 刘美桃, 刘晓东, 韩渊怀. 全基因组关联分析: 基因组学研究的机遇与挑战. 生物技术通报, 2013, 29(6): 1-6.
Zhang Y M, Xing G F, Liu M T, Liu X D, Han Y H. Genome wide association study: opportunities and challenges in genomic research. Biotechnol Bull, 2013, 29(60): 1-6 (in Chinese with English abstract).
[35] 黄琼, 尹继业, 刘昭前. 2型糖尿病全基因组关联分析的研究进展. 中华内分泌代谢杂志, 2010, 26: 432-436.
Huang Q, Yin J Y, Liu Z Q. Progress of genome-wide association studies in type 2 diabetes mellitus. Chin J Endocrinol Metab, 2010, 26: 432-436 (in Chinese with English abstract).
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