植物胆碱单加氧酶基因家族的进化与表达分析

doi:10.3724/SP.J.1006.2026.53046

Abstract

Abstract:

Glycine betaine (GB) is a compatible solute widely found in plants, animals, bacteria, and algae, where it plays a key role in regulating osmotic pressure and maintaining cellular water balance upon accumulation in the cytoplasm. As such, GB is crucial for plant responses to osmotic stress. Choline monooxygenase (CMO) is a rate-limiting enzyme in the biosynthetic pathway of GB in plants. Among 168 genomes analyzed in this study, 131 were found to contain homologous CMO genes, with a total of 169 CMO members identified. Selective pressure analysis revealed that most gene pairs have undergone purifying selection, while only seven gene pairs exhibited signs of positive selection. Phylogenetic analysis classified the CMO gene family into six subfamilies, with positive selection sites detected in five subfamilies except subfamily F. Further analysis of the Rieske domain and Ring-hydroxyl A domain showed a significant positive correlation between their dN/dS values, suggesting co-evolution. qRT-PCR analysis demonstrated that the expression of CMO genes in maize was upregulated under MgSO₄-induced stress. This study provides a comprehensive analysis of the evolution and expression of the CMO gene family, offering a theoretical foundation for the future application of CMO genes in improving crop tolerance to abiotic stress.

Key words: plant glycine betaine, choline monooxygenase gene, selection pressure, purifying selection, maize

Wang Ting, Duan Wu-Li, Wang Rui, Liu Hai-Lan. Evolution and expression analysis of the choline monooxygenase gene family in plants[J].Acta Agronomica Sinica, 2026, 52(1): 44-55.

Figures/Tables 16

Table S1

Genomic data for identifying homologous genes of CMO in plants"

物种名 Species name	基因组数获取的网址 Website for genomic data acquisition
相思子 Abrus precatorius	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/003/935/025/GCF003935025.1Abrus2018/
漾濞槭 Acer yangbiense	http://gigadb.org/dataset/100610
猕猴桃 Actinidia eriantha	http://gigadb.org/dataset/100568
粗山羊草Aegilops tauschii	http://plants.ensembl.org/index.html
欧洲桤木 Alnus glutinosa	http://gigadb.org/dataset/101042
无油樟 Amborella trichopoda	http://plants.ensembl.org/index.html
三色凤梨 Ananas bracteatus	ftp://download.big.ac.cn/gwh/Plants/
菠萝 Ananas comosus	http://plants.ensembl.org/index.html
金鱼草 Antirrhinum majus	http://bioinfo.sibs.ac.cn/Am/downloadv3.php
圆叶拟南芥 Arabidopsis halleri	http://plants.ensembl.org/index.html
琴叶拟南芥 Arabidopsis lyrata	http://plants.ensembl.org/index.html
拟南芥 Arabidopsis thaliana	http://plants.ensembl.org/index.html
高山拟南芥 Arabis alpina	http://plants.ensembl.org/index.html
石刁柏 Asparagus officinalis	http://plants.ensembl.org/index.html
阳桃 Averrhoa carambola	https://bigd.big.ac.cn/search/?dbId=gwh&q=GWHABKE00000000
细叶满江红 Azolla filiculoides	https://www.fernbase.org/
冬瓜 Benincasa hispida	ftp://download.big.ac.cn/gwh/Plants/BenincasahispidaALLPATHS-LGPBJellyGWHAAAR00000000/
甜菜 Beta vulgaris	http://plants.ensembl.org/index.html
木棉树 Bombax ceiba	http://gigadb.org/dataset/100445
芸香竹 Bonia amplexicaulis	http://www.genobank.org/bamboo
二穗短柄草 Brachypodium distachyon	http://plants.ensembl.org/index.html
甘蓝型油菜 Brassica napus	http://plants.ensembl.org/index.html
甘蓝 Brassica oleracea	http://plants.ensembl.org/index.html
白菜 Brassica rapa	http://plants.ensembl.org/index.html
木豆 Cajanus cajan	http://gigadb.org/dataset/100028
茶花 Camelina sativa	http://plants.ensembl.org/index.html
大麻 Cannabis sativa female	http://plants.ensembl.org/index.html
辣椒 Capsicum annuum	http://plants.ensembl.org/index.html
山核桃 Carya cathayensis	http://gigadb.org/dataset/100571
板栗 Castanea mollissima	http://gigadb.org/dataset/view/id/100643
粗枝木麻黄 Casuarina glauca	http://gigadb.org/dataset/101051
珍珠粟 Cenchrus americanus	https://plantgarden.jp/en/list/t4543/genome/t4543.G001
加拿大紫荆 Cercis canadensis	http://gigadb.org/dataset/101044
鹧鸪豌豆 Chamaecrista fasciculata	http://gigadb.org/dataset/101045
无皮轮藻 Chara braunii	http://plants.ensembl.org/index.html
藜麦 Chenopodium quinoa	http://plants.ensembl.org/index.html
柳叶腊梅 Chimonanthus salicifolius	http://xhhuanglab.cn/data/Chimonanthussalicifolius.html
莱茵衣藻 Chlamydomonas reinhardtii	http://plants.ensembl.org/index.html
皱波角叉菜 Chondrus crispus	http://plants.ensembl.org/index.html
菊花脑 Chrysanthemum nankingense	http://www.amwayabrc.com/download.htm
鹰嘴豆 Cicer arietinum	http://gigadb.org/dataset/100076
西瓜 Citrullus lanatus	http://plants.ensembl.org/index.html
金钱橘 Citrus clementina	http://plants.ensembl.org/index.html
椰子 Cocos nuciferacds	http://gigadb.org/dataset/view/id/100347/Filepage/3
中粒咖啡 Coffea canephora	http://plants.ensembl.org/index.html
黄麻 Corchorus capsularis	http://plants.ensembl.org/index.html
甜瓜 Cucumis melo	http://plants.ensembl.org/index.html
黄瓜 Cucumis sativus	http://plants.ensembl.org/index.html
野地菟丝子 Cuscuta campestris	https://www.plabipd.de/projectcuscuta2/start.ep
红藻 Cyanidioschyzon merolae	http://plants.ensembl.org/index.html
刺苞菜蓟 Cynara cardunculus	http://plants.ensembl.org/index.html
野胡萝卜 Daucus carota	http://plants.ensembl.org/index.html
龙眼 Dimocarpus longan	http://gigadb.org/dataset/100276
君迁子 Diospyros lotus	ftp://ftp.kazusa.or.jp/pub/persimmon/
油柿 Diospyros oleifera	http://gigadb.org/dataset/100687
亮黄仙女木 Dryas drummondii	http://gigadb.org/dataset/101047
野稗 Echinochloa crusgalli	http://ibi.zju.edu.cn/RiceWeedomes/Echinochloa/
粗柄象腿蕉 Ensete ventricosum	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/818/735/GCA000818735.3Bedadetiannotated/
弯叶画眉草 Eragrostis curvula	http://plants.ensembl.org/index.html
苔麸 Eragrostis tef	http://plants.ensembl.org/index.html
赤桉 Eucalyptus camaldulensis	ftp://ftp.kazusa.or.jp/pub/eucaly/
大桉 Eucalyptus grandis	http://plants.ensembl.org/index.html
杜仲 Eucommia ulmoides	ftp://download.big.ac.cn/gwh/Plants/Eucommiaulmoideshardyrubberv0GWHAAAL00000000/
荞麦 Fagopyrum esculentum	ftp://ftp.kazusa.or.jp/pub/buckwheat/
天仙果 Ficus erecta	https://plantgarden.jp/en/list/t66383/genome/t66383.G001
粉叶黄毛草莓 Fragaria nilgerrensis	ftp://download.big.ac.cn/gwh/Plants/FragarianilgerrensisTheFragarianilgerrensisgenomefromYunnanGWHABKC00000000/
嗜硫原始红藻 Galdieria sulphuraria	http://plants.ensembl.org/index.html
天麻 Gastrodia elata	ftp://download.big.ac.cn/gwh/Plants/
大豆 Glycine max	http://plants.ensembl.org/index.html
海岛棉 Gossypium barbadense Hai7124	http://ibi.zju.edu.cn/cotton/
海岛棉 Gossypium barbadense TM-1	http://ibi.zju.edu.cn/cotton/
雷蒙德氏棉 Gossypium raimondii	http://plants.ensembl.org/index.html
瓜多竹 Guadua angustifolia	http://www.genobank.org/bamboo
紫花风铃木 Handroanthus impetiginosus	http://gigadb.org/dataset/100379
向日葵 Helianthus annuus	http://plants.ensembl.org/index.html
大麻槿 Hibiscus cannabinus	ftp://download.big.ac.cn/gwh/Plants/HibiscuscannabinusKenafgenomeofFuhong952GWHACDB00000000/
木槿 Hibiscus syriacus	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/006/381/635/GCA006381635.2ASM638163v2/
大麦 Hordeum vulgare	http://plants.ensembl.org/index.html
番薯 Ipomoea batatas	ftp://ftp.kazusa.or.jp/pub/sweetpotato/Mx23Hm/
牵牛 Ipomoea nil	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/879/475/GCA001879475.1Asagao1.1/
三裂叶薯 Ipomoea triloba	http://plants.ensembl.org/index.html
核桃 Juglans regia	http://plants.ensembl.org/index.html
玉吊钟 Kalanchoe fedtschenkoi	http://plants.ensembl.org/index.html
秋茄树 Kandelia obovata	https://bigd.big.ac.cn/search/?dbId=gwh&q=GWHACBH00000000
独叶草 Kingdonia uniflora translated	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/014/058/105/GCA014058105.1ASM1405810v1/
苹果 Malus domestica golden	http://plants.ensembl.org/index.html
杧果 Mangifera indica	ftp://download.big.ac.cn/gwh/Plants/MangiferaindicamangoV1GWHABLA00000000/
木薯 Manihot esculenta	http://plants.ensembl.org/index.html
地钱 Marchantia polymorpha	http://plants.ensembl.org/index.html
蒺藜苜蓿 Medicago truncatula	http://plants.ensembl.org/index.html
小菥蓂 Microthlaspi erraticum	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/902/728/155/GCA902728155.2Merrgenes/
含羞草 Mimosa pudica	http://gigadb.org/dataset/101049
辣木 Moringa oleifera	http://gigadb.org/dataset/101058
香蕉 Musa acuminata	http://plants.ensembl.org/index.html
烟草 Nicotiana attenuata	http://plants.ensembl.org/index.html
丝叶龙舌兰 Nissolia schottii	http://gigadb.org/dataset/101050
蓝星睡莲 Nymphaea colorata	http://plants.ensembl.org/index.html
侏儒卢旺达睡莲 Nymphaea thermarum	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/011/799/765/GCA011799765.1ASM1179976v1/
油橄榄、木犀榄 Olea europaea sylvestris	http://plants.ensembl.org/index.html
莪莉竹 Olyra latifolia	http://www.genobank.org/bamboo
短叶舌野生稻 Oryza barthii	http://plants.ensembl.org/index.html
短花药野生稻 Oryza brachyantha	http://plants.ensembl.org/index.html
非洲栽培稻 Oryza glaberrima	http://plants.ensembl.org/index.html
展颖野生稻 Oryza glumipatula	http://plants.ensembl.org/index.html
籼稻 Oryza sativa indica	http://plants.ensembl.org/index.html
长花药野生稻 Oryza longistaminata	http://plants.ensembl.org/index.html
尼瓦拉野生稻 Oryza nivara	http://plants.ensembl.org/index.html
斑点野生稻 Oryza punctata	http://plants.ensembl.org/index.html
普通野生稻 Oryza rufipogon	http://plants.ensembl.org/index.html
粳稻 Oryza sativa Japonica	http://plants.ensembl.org/index.html
绿色鞭毛藻 Ostreococcus lucimarinus	http://plants.ensembl.org/index.html
柳枝稷 Panicum hallii	http://plants.ensembl.org/index.html
罂粟 Papaver somniferum	http://plants.ensembl.org/index.html
腋花矮牵牛 Petunia axillaris	ftp://ftp.solgenomics.net/genomes/Petuniaaxillaris/annotation/
矮牵牛 Petunia inflata	ftp://ftp.solgenomics.net/genomes/Petuniainflata/
菜豆 Phaseolus vulgaris	http://plants.ensembl.org/index.html
小立碗藓 Physcomitrium patens	http://plants.ensembl.org/index.html
开心果 Pistacia vera	http://plants.ensembl.org/index.html
银白杨 Populus alba	ftp://download.big.ac.cn/gwh/Plants/Populusalbavar.pyramidalisPopulusalbavar.pyramidalisv1GWHAAEP00000000/
欧洲山杨 Populus tremula	ftp://plantgenie.org/Data/PopGenIE/Populustremula/v1.1/
白杨 Populus tremuloides	ftp://plantgenie.org/Data/PopGenIE/Populustremuloides/
毛果杨 Populus trichocarpa	http://plants.ensembl.org/index.html
微甘蓝委陵菜 Potentilla micrantha	http://gigadb.org/dataset/100407
甜樱桃 Prunus avium.PAV	http://plants.ensembl.org/index.html
扁桃 Prunus dulcis	http://plants.ensembl.org/index.html
桃花 Prunus persica.Prunus persica	http://plants.ensembl.org/index.html
星星草 Puccinellia tenuiflora	http://xhhuanglab.cn/data/alkaligrass.html
杜梨 Pyrus betuleafolia	ftp://download.big.ac.cn/gwh/Plants/PyrusbetulifoliaPbe-SDGWHAAYT00000000/
白梨 Pyrus bretschneideri	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/315/295/GCF000315295.1Pbrv1.0/
莪莉竹 Raddia guianensis	http://www.genobank.org/bamboo
马缨杜鹃 Rhododendron delavayi	http://gigadb.org/dataset/100331
月季 Rosa chinensis	http://plants.ensembl.org/index.html
野蔷薇 Rosa multiflora	ftp://ftp.kazusa.or.jp/pub/rosa/
黑树莓 Rubus occidentalis	http://gigadb.org/dataset/100465
甜根子草 Saccharum spontaneum	http://plants.ensembl.org/index.html
甘蔗 Saccharum spp R570	https://sugarcane-genome.cirad.fr/content/download
勺叶槐叶苹 Salvinia cucullata	https://www.fernbase.org/
伯尔硬胡桃 Sclerocarya birrea	http://gigadb.org/dataset/101057
江南卷柏 Selaginella moellendorffii	http://plants.ensembl.org/index.html
芝麻 Sesamum indicum	http://plants.ensembl.org/index.html
小米 Setaria italica	http://plants.ensembl.org/index.html
狗尾草 Setaria viridis	http://plants.ensembl.org/index.html
油蜡树 Simmondsia chinensis	https://bigd.big.ac.cn/search?dbId=gwh&q=GWHAASQ00000000
罗汉果 Siraitia grosvenorii	http://gigadb.org/dataset/100452
番茄 Solanum lycopersicum	http://plants.ensembl.org/index.html
茄 Solanum melongena	ftp://ftp.kazusa.or.jp/pub/eggplant/
番茄 Solanum pennellii	https://www.plabipd.de/projectspenn/start.ep
马铃薯 Solanum tuberosum.SolTub	http://plants.ensembl.org/index.html
高粱 Sorghum bicolor	http://plants.ensembl.org/index.html
密花豆 Spatholobus suberectus	https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/004/329/165/GCA004329165.1ASM432916v1/
菠菜 Spinacia oleracea	http://www.spinachbase.org/?q=download
异子蓬 Suaeda aralocaspica	http://gigadb.org/dataset/view/id/100646/Filepage/2
可可树 Theobroma cacao	http://plants.ensembl.org/index.html
红车轴草 Trifolium pratense	http://plants.ensembl.org/index.html
小麦 Triticum aestivum	http://plants.ensembl.org/index.html
野生二粒小麦 Triticum dicoccoides	http://plants.ensembl.org/index.html
斯卑尔脱小麦 Triticum spelta	http://plants.ensembl.org/index.html
圆锥小麦 Triticum turgidum	http://plants.ensembl.org/index.html
乌拉尔图小麦 Triticum urartu	http://plants.ensembl.org/index.html
赤豆 Vigna angularis	http://plants.ensembl.org/index.html
绿豆 Vigna radiata	http://plants.ensembl.org/index.html
葡萄 Vitis vinifera	http://plants.ensembl.org/index.html
文冠果 Xanthoceras sorbifolium	http://gigadb.org/dataset/view/id/100606/Filepage/3
墨西哥大刍草 Zea Mexicana	https://www.maizegdb.org/
玉米 Zea mays	https://www.maizegdb.org/
结蒌草 Zoysia_japonica	ftp://ftp.kazusa.or.jp/pub/zoysia/
马尼拉草 Zoysia_matrella	ftp://ftp.kazusa.or.jp/pub/zoysia/
细叶结缕草 Zoysia_pacifica	ftp://ftp.kazusa.or.jp/pub/zoysia/

Table S1

Table 1

Table S2

Distribution of CMO gene in plants"

物种名 Species name	基因数量 Number of genes	物种名 Species name	基因数量 Number of genes
菠萝 Ananas comosus	1	辣木 Moringa oleifera	1
粗山羊草 Aegilops tauschii	1	甜菜 Beta vulgaris	2
二穗短柄草 Brachypodium distachyon	1	藜麦 Chenopodium quinoa	4
野稗 Echinochloa crusgalli	3	菠菜 Spinacia oleracea	2
弯叶画眉草 Eragrostis curvula	1	独叶草 Kingdonia uniflora translated	1
苔麸 Eragrostis tef	1	猕猴桃 Actinidia eriantha	1
大麦 Hordeum vulgare	1	粗枝木麻黄 Casuarina glauca	1
短叶舌野生稻 Oryza barthii	1	油橄榄、木犀榄 Olea europaea sylvestris	1
短花药野生稻 Oryza brachyantha	1	葡萄 Vitis vinifera	1
非洲栽培稻 Oryza glaberrima	1	杧果 Mangifera indica	1
展颖野生稻 Oryza glumipatula	1	开心果 Pistacia vera	1
籼稻 Oryza sativa indica	1	漾濞槭 Acer yangbiense	1
长花药野生稻 Oryza longistaminata	1	中粒咖啡 Coffea canephora	1
尼瓦拉野生稻 Oryza nivara	1	亮黄仙女木 Dryas drummondii	1
斑点野生稻 Oryza punctata	1	粉叶黄毛草莓 Fragaria nilgerrensis	1
普通野生稻 Oryza rufipogon	1	苹果 Malus domestica golden	1
柳枝稷 Panicum hallii	1	微甘蓝委陵菜 Potentilla micrantha	1
星星草 Puccinellia tenuiflora	1	扁桃 Prunus dulcis	1
甜根子草 Saccharum spontaneum	4	桃花 Prunus persica.Prunus persica	1
甘蔗 Saccharum spp R570	1	杜梨 Pyrus betuleafolia	1
小米 Setaria italica	1	白梨 Pyrus bretschneideri	1
狗尾草 Setaria viridis	1	月季 Rosa chinensis	1
高粱 Sorghum bicolor	1	野蔷薇 Rosa multiflora	1
小麦 Triticum aestivum	3	黑树莓 Rubus occidentalis	3
野生二粒小麦 Triticum dicoccoides	2	辣椒 Capsicum annuum	1
斯卑尔脱小麦 Triticum spelta	3	烟草 Nicotiana attenuata	1
圆锥小麦 Triticum turgidum	2	番茄 Solanum lycopersicum	1
墨西哥大刍草 Zea Mexicana	1	茄 Solanum melongena	1
玉米 Zea mays	1	番茄 Solanum pennellii	2
丝叶龙舌兰 Nissolia schottii	1	马铃薯 Solanum tuberosum.SolTub	1
三裂叶薯 Ipomoea triloba	1	野胡萝卜 Daucus carota	1
石刁柏 Asparagus officinalis	1	大麻 Cannabis sativa female	1
板栗 Castanea mollissima	1	天仙果 Ficus erecta	2
香蕉 Musa acuminata	1	茶花 Camelina sativa	3
金鱼草 Antirrhinum majus	1	圆叶拟南芥 Arabidopsis halleri	1
木薯 Manihot esculenta	1	琴叶拟南芥 Arabidopsis lyrata	1
相思子 Abrus precatorius	1	拟南芥 Arabidopsis thaliana	1
木豆 Cajanus cajan	1	高山拟南芥 Arabis alpina	2
加拿大紫荆 Cercis canadensis	1	甘蓝型油菜 Brassica napus	2
鹰嘴豆 Cicer arietinum	1	甘蓝 Brassica oleracea	1
大豆 Glycine max	1	白菜 Brassica rapa	1
蒺藜苜蓿 Medicago truncatula	1	小菥蓂 Microthlaspi erraticum	1
含羞草 Mimosa pudica	1	君迁子 Diospyros lotus	2
菜豆 Phaseolus vulgaris	1	油柿 Diospyros oleifera	1
密花豆 Spatholobus suberectus	1	蓝星睡莲 Nymphaea colorata	1
红车轴草 Trifolium pratense	1	侏儒卢旺达睡莲 Nymphaea thermarum	1
赤豆 Vigna angularis	1	赤桉 Eucalyptus camaldulensis	1
绿豆 Vigna radiata	1	大桉 Eucalyptus grandis	2
杜仲 Eucommia ulmoides	1	文冠果 Xanthoceras sorbifolium	2
黄麻 Corchorus capsularis	1	无油樟 Amborella trichopoda	1
秋茄树 Kandelia obovata	1	可可树 Theobroma cacao	1
山核桃 Carya cathayensis	1	番薯 Ipomoea batatas	1
核桃 Juglans regia	2	银白杨 Populus alba	1
冬瓜 Benincasa hispida	1	欧洲山杨 Populus tremula	1
西瓜 Citrullus lanatus	1	白杨 Populus tremuloides	1
甜瓜 Cucumis melo	1	毛果杨 Populus trichocarpa	1
黄瓜 Cucumis sativus	1	罂粟 Papaver somniferum	3
罗汉果 Siraitia grosvenorii	2	油蜡树 Simmondsia chinensis	3
欧洲桤木 Alnus glutinosa	1	金钱橘 Citrus clementina	1
海岛棉 Gossypium barbadense Hai7124	2	芝麻 Sesamum indicum	1
海岛棉 Gossypium barbadense TM-1	2	紫花风铃木 Handroanthus impetiginosus	2
雷蒙德氏棉 Gossypium raimondii	1	阳桃 Averrhoa carambola	1
玉吊钟 Kalanchoe fedtschenkoi	1	柳叶腊梅 Chimonanthus salicifolius	1
菊花脑 Chrysanthemum nankingense	1	江南卷柏 Selaginella moellendorffii	2
刺苞菜蓟 Cynara cardunculus	1	小立碗藓 Physcomitrium patens	1
向日葵 Helianthus annuus	2

Table S2

Table S3

Molecular characteristics of CMO gene family"

序列ID Sequence ID	氨基酸数 Number of amino acids	分子量 Molecular weight	理论pI Theoretical pI	不稳定性指数 Instability index	脂族指数 Aliphatic index	亲水性的总平均值 Grand average of hydropathicity
Abrus precatorius_lcl\|NW 020874428.1 cds XP 027333652.1 35200A	388	44,426.80	7.55	40.01	80.31	−0.268
Acer yangbiense_Acyan05G0044100.1	407	45,991.44	5.84	34.55	78.77	−0.217
Actinidia eriantha_DTZ79 01g10130	499	56,655.53	6.19	37.35	84.97	−0.216
Aegilops tauschii_AET7Gv21027100.10	384	43,303.83	7.93	46.95	74.14	−0.366
Alnus glutinosa_Alngl15693S17170	424	48,283.89	6.24	50.18	79.53	−0.288
Amborella trichopoda_ERN07450	422	47,702.22	6.23	41.94	80.40	−0.200
Ananas comosus_Aco012177.1.mrna1	420	46,648.75	6.06	41.48	81.79	−0.174
Antirrhinum majus_Am07g29560.T02	420	47,806.01	5.46	40.42	73.55	−0.304
Arabidopsis halleri_g04236.t1	423	47,829.34	6.32	34.25	75.48	−0.259
Arabidopsis lyrata_Al scaffold 0007 1121	422	47,612.95	6.40	33.97	75.21	−0.292
Arabidopsis thaliana_AT4G29890.1	422	47,724.20	6.40	34.62	75.00	−0.267
Arabis alpina_KFK40841	236	26,405.74	4.92	34.18	74.66	−0.122
Arabis alpina_KFK29548	420	47,609.13	5.98	37.50	76.76	−0.225
Asparagus officinalis_ONK69555	320	36,247.06	5.70	36.12	84.69	−0.204
Averrhoa carambola_GWHTABKE015297	384	43,481.68	5.76	32.64	88.57	−0.138
Benincasa hispida_GWHTAAAR012286	417	47,165.43	6.01	41.06	81.58	−0.263
Beta vulgaris_KMS95013	418	47,721.94	6.08	39.51	78.11	−0.290
Beta vulgaris_KMT07791	446	50,218.75	6.02	42.91	73.05	−0.414
Brachypodium distachyon_KQK17361	409	45,443.46	5.77	37.45	84.69	−0.146
Brassica napus_CDY30186	429	48,768.32	6.57	41.34	74.24	−0.338
Brassica napus_CDY46714	425	47,706.96	5.98	39.40	76.56	−0.298
Brassica oleracea_Bo7g114080.1	438	49,047.26	5.77	40.72	76.96	−0.295
Brassica rapa_Bra024118.1	413	46,710.85	6.40	39.10	74.77	−0.344
Cajanus cajan_C.cajan 25086	416	47,372.76	6.31	42.05	75.60	−0.299
Camelina sativa_Csa12g017360.1	427	48,362.86	6.20	36.43	71.17	−0.326
Camelina sativa_Csa11g013650.1	617	68,956.12	6.27	33.67	73.10	−0.291
Camelina sativa_Csa10g012700.1	691	77,759.06	7.03	35.57	71.61	−0.336
Cannabis sativa female_evm.model.06.1887	295	33,355.51	8.61	34.42	83.19	−0.086
Capsicum annuum_PHT75633	497	56,063.43	7.58	36.79	87.85	−0.086
Carya cathayensis_CIL1068S0063	432	49,240.95	6.38	44.67	79.63	−0.301
Castanea mollissima_BUA.CMHBY200043	310	34,967.08	8.22	40.13	83.29	−0.171
Casuarina glauca_Casgl141S08699	459	51,750.59	5.74	44.24	78.39	−0.247
Cercis canadensis_Cerca1S17463	388	44,131.33	6.20	43.02	82.86	−0.202
Chenopodium quinoa_AUR62031264-RA	304	34,241.85	5.81	30.23	83.36	−0.202
Chenopodium quinoa_AUR62032506-RA	435	48,595.97	6.06	42.70	74.18	−0.317
Chenopodium quinoa_AUR62043230-RA	433	48,469.71	5.72	44.30	74.76	−0.324
Chenopodium quinoa_AUR62027431-RA	421	47,732.94	5.44	35.10	83.30	−0.214
Chimonanthus salicifolius_Cs01g01842	381	42,964.78	5.25	36.81	82.91	−0.127
Chrysanthemum nankingense_CHR00024703-RA	406	46,137.13	5.59	38.40	78.25	−0.312
Cicer arietinum_Ca 03209	414	46,735.03	6.27	42.85	78.33	−0.293
Citrullus lanatus_Cla97C03G062150.1	417	47,190.43	6.01	39.36	80.86	−0.249
Citrus clementina_ESR66460	408	46,131.30	5.50	36.96	82.16	−0.210
Coffea canephora_CDP08949	431	49,346.07	6.78	35.41	78.89	−0.349
Corchorus capsularis_OMO52515	319	36,135.18	6.00	41.88	81.29	−0.195
Cucumis melo_MELO3C009992.2.1	417	47,485.84	6.11	37.31	79.47	−0.287
Cucumis sativus_KGN66608	417	47,255.67	6.05	35.10	82.04	−0.245
Cynara cardunculus_KVI11819	394	45,199.09	6.78	40.59	87.06	−0.167
Daucus carota_KZM92492	1464	16,4552.7	5.65	46.54	78.82	−0.477
Diospyros lotus_Dlo pri0547F.1 g00580.1	446	50,158.65	6.64	47.03	73.21	−0.300
Diospyros lotus_Dlo pri0547F.1 g00640.1	393	43,994.53	6.11	52.70	73.41	−0.257
Diospyros oleifera_evm.model.fragScaff scaffold 92.1300 evm.model.fragScaff scaffold 92.1301	421	47,222.44	7.06	45.60	73.14	−0.287
Dryas drummondii_Drydr37S21113	408	46,063.16	5.79	33.25	75.71	−0.306
Echinochloa crusgalli_scaffold225.242	404	44,809.47	6.05	37.99	78.24	−0.226
Echinochloa crusgalli_scaffold60.245	404	45,015.79	5.81	39.67	80.67	−0.195
Echinochloa crusgalli_scaffold9.745	402	44,698.32	5.96	41.01	78.41	−0.240
Eragrostis curvula_TVU07759	409	45,451.22	5.82	32.29	81.83	−0.184
Eragrostis tef_Et s5159-1.44-1.mrna1	383	42,226.80	6.19	35.86	78.25	−0.198
Eucalyptus camaldulensis_EcC049426.60	412	46,208.76	6.59	35.67	83.11	−0.168
Eucalyptus grandis_KCW78622	429	48,260.9-	6.21	36.30	82.07	−0.232
Eucalyptus grandis_KCW78623	317	35,414.28	6.88	42.38	77.85	−0.248
Eucommia ulmoides_GWHTAAAL012211	313	35,652.59	6.42	38.14	81.25	−0.258
Ficus erecta_Fer r1.1chr01b g000830.1	393	43,744.51	5.73	38.98	78.37	−0.231
Ficus erecta_Fer1.1hctg g093930.1	410	46,082.28	5.63	37.17	79.15	−0.219
Fragaria nilgerrensis_GWHTABKC012960	414	46,874.23	5.97	36.42	79.32	−0.29
Glycine max_KRH02754	418	47,845.48	7.90	35.83	77.13	−0.381
Gossypium barbadense Hai7124_GB A05G4385	439	49,992.50	6.03	38.42	79.07	−0.325
Gossypium barbadense Hai7124_GB D04G0080	407	46,197.25	5.97	35.22	77.86	−0.284
Gossypium barbadense TM-1_GH A05G4292	409	46,493.66	5.97	35.07	79.63	−0.264
Gossypium barbadense TM-1_GH D04G0081	407	46,212.26	5.87	36.17	77.86	−0.284
Gossypium raimondii_KJB74798	411	46,728.73	5.68	35.99	76.86	−0.319
Handroanthus impetiginosus_Haimp10008576m	439	49,923.51	6.18	38.79	72.85	−0.351
Handroanthus impetiginosus_Haimp10031904m	439	49,856.46	6.25	38.37	74.83	−0.314
Helianthus annuus_OTG23339	410	46,604.07	6.14	32.99	81.29	−0.196
Helianthus annuus_OTG23340	459	52,375.47	5.97	40.36	77.49	−0.282
Hordeum vulgare_HORVU7Hr1G097970.8	361	40,218.39	5.59	38.78	81.86	−0.192
Ipomoea batatas_Itr sc001900.1 g00002.1	414	46,542.74	5.83	41.54	77.68	−0.220
Ipomoea triloba_itb13g16230.t1	421	47,270.46	5.50	40.95	78.48	−0.204
Juglans regia_Jr11 26260 p1	428	48,689.53	6.64	44.28	81.07	−0.290
Juglans regia_Jr11 26250 p1	430	48,902.77	6.64	44.12	81.37	−0.287
Kalanchoe fedtschenkoi_Kaladp0809s0111.2.v1.1	462	51,675.05	6.00	39.59	77.88	−0.274
Kandelia obovata_GWHTACBH001622	553	62,890.92	6.98	42.13	80.71	−0.138
Kingdonia uniflora translated_lcl\|JACGCM010001097.1 cds KAF6161747.1 37800	423	47,750.42	6.14	36.92	83.40	−0.203
Malus domestica golden_mRNA:MD13G0209900	405	45,902.03	6.27	38.10	74.84	−0.326
Mangifera indica_GWHTABLA026339	410	46,769.59	6.28	29.96	80.78	−0.248
Manihot esculenta_OAY46667	417	47,169.66	6.37	33.63	76.64	−0.283
Medicago truncatula_AES92225	411	46,601.76	6.02	44.97	75.60	−0.365
Microthlaspi erraticum_lcl\|CACVBM020001396.1 cds CAA7048834.1 36068	422	47,879.50	5.88	37.06	75.05	−0.248
Mimosa pudica_Mimpu2389S20482	409	46,395.69	6.25	35.45	83.13	−0.255
Moringa oleifera_Morol34346S06261 MOROL	467	53,023.26	6.88	38.42	76.81	−0.306
Musa acuminata_Ma07 t21070.1	425	47,566.22	6.85	41.15	81.62	−0.134
Nicotiana attenuata_OIT26339	480	54,484.74	6.80	34.85	75.31	−0.370
Nissolia schottii_Nissc300S07994	410	46,648.90	6.02	44.11	75.78	−0.332
Nymphaea colorata_NC3G0230150.1	410	45,945.85	5.77	41.67	76.85	−0.265
Nymphaea thermarum_lcl\|JAANDH010000749.1 cds KAF3777953.1 18956	436	49,065.48	6.10	44.01	76.49	−0.283
Olea europaea sylvestris_Oeu035450.1	436	49,685.34	5.74	37.76	78.21	−0.297
Oryza barthii_OBART06G26220.3	375	41,971.26	6.26	37.37	75.73	−0.279
Oryza brachyantha_OB06G33830.1	305	34,751.22	5.43	29.43	76.07	−0.305
Oryza glaberrima_ORGLA06G0218800.1	410	45,685.57	6.27	38.89	78.56	−0.228
Oryza glumipatula_OGLUM06G27620.1	411	45,744.59	6.05	39.91	77.42	−0.232
Oryza sativa indica_BGIOSGA023545-TA	410	45,660.55	6.05	38.57	78.56	−0.209
Oryza longistaminata_KN538794.1 FGT003	425	47,860.75	4.91	39.44	78.02	−0.309
Oryza nivara_ONIVA06G28770.1	411	45,743.64	6.05	39.91	78.37	−0.213
Oryza punctata_OPUNC06G23800.1	498	55,960.26	6.18	45.79	76.69	−0.313
Oryza rufipogon_ORUFI06G28150.1	411	45,715.59	5.95	39.73	78.37	−0.210
Panicum hallii_PUZ59274	407	45,304.24	5.96	36.57	81.03	−0.202
Papaver somniferum_RZC56707	315	35,126.77	5.46	37.69	78.92	−0.161
Papaver somniferum_RZC71240	332	36,842.53	5.35	43.00	80.18	−0.171
Papaver somniferum_RZC75460	339	39,190.38	8.88	27.79	74.72	−0.354
Phaseolus vulgaris_ESW26689	282	31,985.18	7.62	35.56	72.16	−0.349
Physcomitrium patens_Pp3c13 13580V3.4	451	49,987.43	6.06	49.99	76.76	−0.217
Pistacia vera_EVM0007850.1	375	42,913.04	6.36	36.60	76.91	−0.293
Populus alba_GWHTAAEP028330	424	47,982.36	5.79	34.74	83.21	−0.257
Populus tremula_Potra002430g18449.1	424	48,022.42	5.82	34.41	83.21	−0.255
Populus tremuloides_Potrs005543g07870.1	249	27,910.64	5.44	33.11	84.94	−0.143
Populus trichocarpa_PNT30270	467	52,921.30	5.82	37.57	87.24	−0.166
Potentilla micrantha_28126 t	412	46,553.93	6.36	35.68	74.71	−0.330
Prunus dulcis_VVA22231	414	46,744.00	5.91	38.15	76.04	−0.307
Prunus persica.Prunus persica_ONI27175	414	46,775.10	6.01	39.07	76.98	−0.314
Puccinellia tenuiflora_Pt Chr0401059	410	45,827.76	5.63	38.87	82.37	−0.189
Pyrus betuleafolia_GWHTAAYT015348	405	45,877.03	5.96	36.93	74.59	−0.309
Pyrus bretschneideri_lcl\|NW 008988770.1 cds XP 009343850.1 40199	406	46,036.22	6.07	35.95	74.66	−0.327
Rosa chinensis_PRQ37103	410	46,499.79	6.24	34.86	72.95	−0.374
Rosa multiflora_Rmu ssc0000064.1 g000028.1	494	55,680.09	8.31	37.26	69.84	−0.486
Rubus occidentalis_Ro04 G13963	393	44,640.76	5.81	34.27	80.05	−0.249
Rubus occidentalis_Ro04 G13961	398	45,543.24	6.27	32.11	79.50	−0.130
Rubus occidentalis_Ro04 G36994	439	49,902.83	6.07	33.43	78.54	−0.269
Saccharum spontaneum_Sspon.08G0002390-4D-mRNA-1	472	52,679.72	5.80	39.09	83.33	−0.209
Saccharum spontaneum_Sspon.08G0002390-2B-mRNA-1	497	55,071.88	5.21	40.36	79.13	−0.254
Saccharum spontaneum_Sspon.08G0002390-3C-mRNA-1	426	47,242.18	5.78	36.40	78.12	−0.262
Saccharum spontaneum_Sspon.08G0002390-1A-mRNA-1	412	45,699.59	5.77	35.69	80.53	−0.185
Saccharum spp R570_Sh10 t017070	440	48,737.27	6.57	38.83	82.27	−0.151
Selaginella moellendorffii_EFJ17750	410	45,816.94	6.36	44.22	81.59	−0.228
Selaginella moellendorffii_EFJ16278	410	45,847.90	6.23	43.60	80.39	−0.258
Sesamum indicum_SIN 1003525.t	430	48,784.68	6.85	44.19	78.19	−0.245
Setaria italica_KQL11792	411	45,582.43	5.78	37.14	79.32	−0.221
Setaria viridis_TKW22888	411	45,601.43	5.87	37.40	79.32	−0.227
Simmondsia chinensis_GWHTAASQ009711	421	47,619.84	6.46	42.96	76.86	−0.350
Simmondsia chinensis_GWHTAASQ009712	389	44,074.98	6.91	43.10	77.17	−0.351
Simmondsia chinensis_GWHTAASQ009713	316	35,527.14	7.14	40.10	76.52	−0.321
Siraitia grosvenorii_evm.TU.tig00001789.1	1149	129,249.30	8.88	38.84	79.57	−0.549
Siraitia grosvenorii_evm.TU.tig00153047.352	580	65,709.65	8.36	39.13	86.00	−0.134
Solanum lycopersicum_Solyc07g008300.2.1	791	90,114.56	6.63	35.53	82.04	−0.208
Solanum melongena_Sme2.5 00354.1 g00012.1	321	35,716.77	6.63	31.59	88.29	−0.156
Solanum pennellii_Sopen07g004190.1	363	41,018.23	5.23	39.76	79.70	−0.268
Solanum pennellii_Sopen07g004200.1	412	46,683.02	6.20	35.84	76.63	−0.244
Solanum tuberosum.SolTub_PGSC0003DMT400051201	412	46,827.11	6.11	36.97	75.66	−0.256
Sorghum bicolor_OQU76986	405	45,068.85	5.96	34.75	79.98	−0.194
Spatholobus suberectus_lcl\|CM014734.1 cds TKY54511.1 18660	310	35,336.97	7.11	33.28	71.61	−0.365
Spinacia oleracea_Spo13645	437	49,056.18	5.73	44.35	70.94	−0.431
Spinacia oleracea_Spo25467	321	36,406.12	5.49	32.25	79.22	−0.311
Theobroma cacao_EOY05290	407	46,322.69	5.49	39.57	84.30	−0.168
Trifolium pratense_Tp57577 TGAC v2 mRNA5577	432	48,959.60	8.12	35.71	76.67	−0.351
Triticum aestivum_TraesCS7A02G425000.1	409	45,026.73	5.54	42.36	81.64	−0.162
Triticum aestivum_TraesCS7D02G416700.1	403	44,397.09	5.70	43.35	83.08	−0.153
Triticum aestivum_TraesCS7B02G324900.1	410	44,942.74	5.84	44.22	80.73	−0.124
Triticum dicoccoides_TRIDC7BG051890.1	411	45,046.76	5.64	44.39	79.59	−0.145
Triticum dicoccoides_TRIDC7AG059040.26	406	45,181.15	5.65	44.38	84.78	−0.209
Triticum spelta_TraesTSP7B01G348200.1	410	449,42.74	5.84	44.22	80.73	−0.124
Triticum spelta_TraesTSP7A01G456800.1	409	45,026.73	5.54	42.36	81.64	−0.162
Triticum spelta_TraesTSP7D01G455400.1	403	44,388.08	5.64	43.35	83.08	−0.154
Triticum turgidum_TRITD7Bv1G178280.2	388	42,494.90	5.65	42.72	80.77	−0.145
Triticum turgidum_TRITD7Av1G228650.5	409	45,026.73	5.54	42.36	81.64	−0.162
Vigna angularis_KOM29586	416	47,546.17	6.70	43.03	78.68	−0.306
Vigna radiata_Vradi07g22700.1	348	39,760.25	7.96	40.03	75.29	−0.327
Vitis vinifera_VIT 11s0052g00330.t01	441	49,558.18	7.56	41.20	77.39	−0.310
Xanthoceras sorbifolium_EVM0015788.1	365	41,192.00	7.17	29.01	76.88	−0.192
Xanthoceras sorbifolium_EVM0018042.1	366	41,313.86	5.58	37.06	79.92	−0.211
Zea Mexicana_ZMex06t021860 03	389	43,468.00	5.41	35.14	80.00	−0.214
Zea mays_Zm00001eb273050 T002	433	48,549.04	6.04	37.51	79.08	−0.198

Table S3

Fig. 1

Table 2

Estimation of dN/dS values and likelihood ratio test of the CMO gene family under site models"

亚家族 Subfamily	模型 Model	dN/dS (ω)	参数估计 Estimates of parameters	正选择位点个数Number of positively selected sites	似然值检验P值 P-value of Likelihood ratio test
A	M0	0.261	ω = 0.261	None	2Δlnl (M0 vs M3) = 1320.096, P < 0.001
	M3	0.314	p₀ = 0.259, ω₀= 0.015; p₁= 0.516, ω₁= 0.213; p₂= 0.226, ω₂= 0.890	—	2Δlnl (M0 vs M3) = 1320.096, P < 0.001
	M1	0.361	p₀= 0.744, ω₀= 0.142; p₁= 0.256, ω₁= 1.000	Not allowed	2Δlnl (M1 vs M2) = 0.000, P = 1.000
	M2	0.361	p₀= 0.744, ω₀= 0.142; p₁= 0.069, ω₁= 1.000; p₂= 0.187, ω₂= 1.000	—	2Δlnl (M1 vs M2) = 0.000, P = 1.000
	M7	0.306	p = 0.478, q = 1.081	Not allowed	2Δlnl (M7 vs M8) = 40.802, P < 0.001
	M8	0.320	p₀= 0.892 (p₁= 0.108), p = 0.680; q = 2.434, ω = 1.185	3	2Δlnl (M7 vs M8) = 40.802, P < 0.001
B	M0	0.228	ω = 0.228	None	2Δlnl (M0 vs M3) = 835.826, P < 0.001
	M3	0.349	p₀= 0.509, ω₀= 0.044; p₁= 0.378, ω₁= 0.354; p₂= 0.113, ω₂= 1.704	—	2Δlnl (M0 vs M3) = 835.826, P < 0.001
	M1	0.323	p₀= 0.760, ω₀= 0.109; p₁= 0.240, ω₁= 1.000	Not allowed	2Δlnl (M1 vs M2) = 43.476, P < 0.001
	M2	0.459	p₀=0.749, ω₀=0.116; p₁= 0.211, ω₁= 1.000; p₂= 0.040, ω₂= 4.004	6	2Δlnl (M1 vs M2) = 43.476, P < 0.001
	M7	0.279	p = 0.371, q = 0.953	Not allowed	2Δlnl (M7 vs M8) = 78.597, P < 0.001
	M8	0.369	p₀= 0.926 (p₁= 0.074), p = 0.519; q = 1.883, ω = 2.312	8	2Δlnl (M7 vs M8) = 78.597, P < 0.001
C	M0	0.244	ω = 0.244	None —	2Δlnl (M0 vs M3) = 1432.434, P < 0.001
	M3	0.352	p₀= 0.481, ω₀= 0.054; p₁= 0.377, ω₁= 0.346 p₂= 0.142, ω₂=1.381	None —	2Δlnl (M0 vs M3) = 1432.434, P < 0.001
	M1	0.361	p₀= 0.735, ω₀= 0.131; p₁= 0.265, ω₁= 1.000	Not allowed	2Δlnl (M1 vs M2) = 34.748, P < 0.001
	M2	0.460	p₀= 0.720, ω₀= 0.138; p₁= 0.222, ω₁= 1.000; p₂= 0.058, ω₂= 2.390	4	2Δlnl (M1 vs M2) = 34.748, P < 0.001
	M7	0.304	p = 0.436, q = 0.992	Not allowed	2Δlnl (M7 vs M8) = 93.993, P < 0.001
	M8	0.364	p₀= 0.889 (p₁= 0.111), p = 0.658; q = 2.455, ω = 1.610	20	2Δlnl (M7 vs M8) = 93.993, P < 0.001
D	M0	0.260	ω = 0.260	None	2Δlnl (M0 vs M3) = 1697.515, P < 0.001
	M3	0.377	p₀= 0.469, ω₀= 0.042; p₁= 0.381, ω₁= 0.344; p₂= 0.150, ω₂= 1.510	—	2Δlnl (M0 vs M3) = 1697.515, P < 0.001
	M1	0.371	p₀= 0.706, ω₀= 0.108	Not allowed	2Δlnl (M1 vs M2) = 106.877, P < 0.001
	M2	0.508	p₀= 0.691, ω₀= 0.113; p₁= 0.258, ω₁= 1.000; p₂= 0.051, ω₂= 3.345	1	2Δlnl (M1 vs M2) = 106.877, P < 0.001
	M7	0.317	p = 0.368, q = 0.790	Not allowed	2Δlnl (M7 vs M8) = 135.394, P < 0.001
	M8	0.403	p₀= 0.930 (p₁= 0.070), p = 0.469; q = 1.379, ω = 2.402	1	2Δlnl (M7 vs M8) = 135.394, P < 0.001
E	M0	0.313	ω = 0.313	None —	2Δlnl (M0 vs M3) = 237.886, P < 0.001
	M3	0.377	p₀= 0.553, ω₀= 0.026; p₁= 0.340, ω₁= 0.486; p₂= 0.107, ω₂= 1.839	None —	2Δlnl (M0 vs M3) = 237.886, P < 0.001
	M1	0.336	p₀= 0.711, ω₀= 0.067; p₁= 0.289, ω₁= 1.000	Not allowed	2Δlnl (M1 vs M2) = 7.991, P = 0.018
	M2	0.395	p₀= 0.711, ω₀= 0.072; p₁= 0.258, ω₁= 1.000; p₂= 0.032, ω₂= 2.699	—	2Δlnl (M1 vs M2) = 7.991, P = 0.018
	M7	0.316	p = 0.162, q = 0.351	Not allowed	2Δlnl (M7 vs M8) = 16.603, P < 0.001
	M8	0.377	p₀= 0.921 (p₁= 0.079), p = 0.268; q = 0.857, ω = 2.007	2	2Δlnl (M7 vs M8) = 16.603, P < 0.001
F	M0	0.187	ω = 0.187	None	2Δlnl (M0 vs M3) = 158.336, P < 0.001
	M3	0.224	p₀= 0.220, ω₀= 0; p₁= 0.621, ω₁= 0.170; p₂= 0.168, ω₂= 0.703	—	2Δlnl (M0 vs M3) = 158.336, P < 0.001
	M1	0.276	p₀= 0.828, ω₀= 0.126; p₁= 0.172, ω₁= 1.000	Not allowed	2Δlnl (M1 vs M2) = 0.000, P = 1.000
	M2	0.276	p₀= 0.828, ω₀= 0.126; p₁= 0.043, ω₁= 1.000; p₂= 0.130, ω₂= 1.000	—	2Δlnl (M1 vs M2) = 0.000, P = 1.000
	M7	0.217	p = 0.690, q = 2.456	Not allowed	2Δlnl (M7 vs M8) = 1.821, P = 0.402
	M8	0.225	p₀= 0.950 (p₁= 0.050), p = 0.838, q = 3.627, ω = 1.000	—	2Δlnl (M7 vs M8) = 1.821, P = 0.402

Table 2

Fig. 2

Table 3

Fig. 3

Table 4

Table 5

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

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序列1 Sequence 1	序列2 Sequence 2	dN/dS值 dN/dS value
Brassica napus__CDY30186	Brassica rapa__Bra024118.1	1.113
Oryza glaberrima__ORGLA06G0218800.1	Oryza glumipatula__OGLUM06G27620.1	1.868
Oryza glaberrima__ORGLA06G0218800.1	Oryza sativa indica__BGIOSGA023545-TA	1.866
Oryza glaberrima__ORGLA06G0218800.1	Oryza nivara__ONIVA06G28770.1	1.602
Oryza glaberrima__ORGLA06G0218800.1	Oryza rufipogon__ORUFI06G28150.1	2.140
Rubus occidentalis__Ro04 G13961	Rubus occidentalis__Ro04 G36994	1.175
Saccharum spontaneum__Sspon.08G0002390-4DmRNA-1	Saccharum spontaneum__Sspon.08G0002390-2BmRNA-1	1.002

物种 Species	文献 Reference	物种 Species	文献 Reference
甜菜Beta vulgaris	[10]	黄瓜Cucumis sativus	[25]
大麦Hordeum vulgare	[10]	粉叶黄毛草莓Fragaria nilgerrensis	[26]
高粱Sorghum bicolor	[10]	大豆Glycine max	[27]
菠菜Spinacia oleracea	[10]	向日葵Helianthus annuus	[28]
小麦Triticum aestivum	[10]	番薯Ipomoea batatas	[29]
玉米Zea mays	[10]	甘蔗Saccharum officinarum	[30]
黎麦Chenopodium quinoa	[23]	绿豆Vigna radiata	[31]
西瓜Citrullus lanatus	[24]	葡萄Vitis vinifera	[32]

物种 Species	文献 Reference	物种 Species	文献 Reference
番茄Solanum lycopersicum	[10]	烟草Nicotiana tabacum	[10]
马铃薯Solanum tuberosum	[10]	粳稻Oryza sativa Japonica	[10,33]
拟南芥Arabidopsis thaliana	[10]	毛果杨Populus trichocarpa	[34]

Evolution and expression analysis of the choline monooxygenase gene family in plants

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