花生磷脂酰肌醇转运蛋白基因AhSFH的克隆及其响应黄曲霉菌侵染的表达特征分析

doi:10.3724/SP.J.1006.2025.44191

Abstract

Abstract:

Phosphatidylinositol transfer proteins (PITPs) are a class of proteins responsible for transporting phosphatidylinositol and phosphatidylcholine monomers across the inner membrane systems of eukaryotic cells. They play essential roles in plant growth and development, signal transduction, stress responses, and other vital biological processes. To date, the involvement of PITP genes in peanut (Arachis hypogaea) responses to Aspergillus flavus infection has not been reported. In this study, the PITP gene was cloned from the highly resistant peanut variety “J11” using RT-PCR, and its molecular characterization and functional prediction were analyzed through bioinformatics, RT-qPCR, and subcellular localization studies. The results showed that the gene’s coding region is 1836 bp in length, encoding an unstable hydrophilic protein with the molecular formula C₃₁₁₄H₄₉₃₈N₈₈₀O₉₄₃S₃₅. The protein consists of 611 amino acids, with a molecular weight of 70.91 kD and an isoelectric point of 7.84. It lacks signal peptide and transmembrane domains but contains typical Sec14 and nodulin domains. It belongs to the SFH subfamily of the plant PITP family and is closely related to soybean and ricinus SFH proteins. Subcellular localization analysis indicated that the AhSFH protein is primarily localized in the cytoplasm. Promoter cis-acting element analysis revealed that the AhSFH promoter contains large number of light-, hormone-, and stress-responsive elements. Transcriptome and RT-qPCR analyses showed that AhSFH expression increased sharply in resistant materials during the early stages of A. flavus infection (T2–T3), surpassing the expression levels observed in highly susceptible materials. Additionally, protein interaction prediction suggested that AhSFH is associated with several transferase-related family proteins. These findings indicate that the AhSFH gene in peanuts plays a crucial role in responding to A. flavus infection and may function as a positive regulator in enhancing peanut resistance to this pathogen.

Key words: peanut, phosphatidylinositol transport protein, AhSFH, Aspergillus flavus, expression analysis

GUO Teng-Da, CUI Meng-Jie, CHEN Lin-Jie, HAN Suo-Yi, GUO Jing-Kun, WU Chen-Di, FU Liu-Yang, HUANG Bing-Yan, DONG Wen-Zhao, ZHANG Xin-You. Cloning and expression analysis of the phosphatidylinositol transfer protein AhSFH gene in peanuts responsive to Aspergillus flavus infection[J].Acta Agronomica Sinica, 2025, 51(6): 1489-1500.

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Cloning and expression analysis of the phosphatidylinositol transfer protein AhSFH gene in peanuts responsive to Aspergillus flavus infection

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