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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1818-1826.doi: 10.3724/SP.J.1006.2012.01818

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development of Genome-Specific Molecular Markers for Lophopyrum elongatum Based on Suppression Subtractive Hybridization

GE Jiang-Yan,CHEN Shi-Qiang,GAO Ying-Ying,GAO Yong,ZHU Xue,HUANG Ze-Feng,CHEN Jian-Min*   

  1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
  • Received:2012-02-23 Revised:2012-06-06 Online:2012-10-12 Published:2012-07-03
  • Contact: 陈建民, E-mail: jmchen@yzu.edu.cn, Tel: 0514-87979286

Abstract:

The unidirectional subtracted library of the testerLophopyrum elongatum (2n=2x=14, EE) and the driver wheat (Triticum aestivum cv. Chinese Spring, 2n=6x=42, AABBDD) was constructed by suppression subtractive hybridization (SSH) method to remove the homologous sequences and enrich the difference of sequences between L. elongatum and Chinese Spring. After cloning and sequencing the specific fragments of L. elongatum, we obtained 36 genome-specific molecular markersof L. elongatum with the efficiency of 69.2%. These markers could be stably inherited in different wheat backgrounds and different hybrid generations with L. elongatum chromosomes, indicating that they are applicable in the detection of chromosomes or chromosomal fragments of L. elongatum in wheat background.

Key words: Triticum aestivum, Lophopyrum elongatum, Genome-specific molecular marker, Suppression subtractive hybridization

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