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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (11): 1905-1913.doi: 10.3724/SP.J.1006.2014.01905

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Functional Analysis of Photo-Oxidative Stress Responsive Genes in Wheat Using Virus-Induced Gene Silencing System

CHEN Kun-Mei1,2,LI Hong-Wei2,*,LIN Fan-Yun2,CHEN Yao-Feng1,*, LI Bin2, ZHENG Qi2,LI Zhen-Sheng2   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2014-03-03 Revised:2014-09-16 Online:2014-11-12 Published:2014-10-01

Abstract:

Functional analysis of photo-oxidative stress responsive genes in wheat (Triticum aestivum L.) may benefit wheat improvement for high radiation use efficiency. A Chinese variety Xiaoyan 54 developed from distant hybridization between common wheat (T. aestivum, 2n=42) and tall wheatgrass (Thinopyrum ponticum, 2n=70) shows significant tolerance to high light induced photo-oxidative stress. Based on previous transcriptome analysis of Xiaoyan 54 in response to high light stress, six genes were selected in this study to assess their possible roles in photo-oxidative stress response using barley stripe mosaic virus (BSMV) mediated virus-induced gene silencing (VIGS) system in Xiaoyan 54. The BSMV induced silencing of the targeted genes together with the BSMV:GFP control plants were exposed to low temperature and high lightII (Fv/Fm), the photosynthetic performance index (P.I.), malondialdehyde (MDA) content, and biomass were evaluated. The results showed that Ta23008 and Ta92165 were involved in the responses of wheat to LTHL, DCMU, MV, and H2O2, respectively. Ta106078 was responsible for wheat tolerance to DCMU, MV, and H2O2 while Ta27787 was responsible for LTHL, DCMU, and H2O2 stress. Ta24695 participated in the response of wheat to both LTHL and H2O2. However, Ta119251 seemed to be only responsible for the DCMU stress in wheat. Additionally, four genes, Ta23008, Ta92165, Ta106078 and Ta119251, were likely to regulate biomass accumulation because the biomass was significantly reduced when they were silenced in wheat. These results provided new hints toward understanding the molecular mechanism of tolerance to photo-oxidative stress in Xiaoyan 54.(LTHL), N-(3,4-dichlorophenyl)- N’,N’-dimethylurea(DCMU), methylviologen (MV), and hydrogen peroxide (H2O2) stress, respectively. The maximum photochemical efficiency of photosystem

Key words: Triticum aestivum, Barley stripe mosaic virus, VIGS, Genomic function, Photo-oxidative stress

[1]刘道宏. 高光效育种. 湖北农业科学, 1978, (6): 35–40



   Liu D H. High photosynthesis efficiency breeding. Hubei Agric Sci, 1978, (6): 35–40 (in Chinese)



[2]吴琼, 许为钢, 李艳, 齐学礼, 胡琳, 张磊, 韩琳琳. 田间条件下转玉米C4型PEPC基因小麦的光合生理特性. 作物学报, 2011, 37: 2046–2052



   Wu Q, Xu W G, Li Y, Qi X L, Hu L, Zhang L, Han L L. Physiological characteristics of photosynthesis in transgenic wheat with maize C4-PEPC gene under field conditions. Acta Agron Sin, 2011, 37: 2046–2052 (in Chinese with English abstract)



[3]程建峰, 沈允钢. 作物高光效之管见. 作物学报, 2010, 36: 1235–1247



   Cheng J F, Shen Y G. My humble opinions on high photosynthetic efficiency of crop. Acta Agron Sin, 2010, 36: 1235–1247 (in Chinese with English abstract)



[4]Unver T, Budak H. Virus-induced gene silencing, a post transcriptional gene silencing method. Int J Plant Genomics, 2009, 2009: 1–8



[5]Holzberg S, Brosio P, Gross C, Pogue G P. Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J, 2002, 30: 315–327



[6]Scofield S R, Huang L, Brandt A S, Gill B S. Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiol, 2005, 138: 2165–2173



[7]Eck L V, Schultz T, Leach J E, Scofield S R, Peairs F B, Botha A M, Lapitan N L V. Virus-induced gene silencing of WRKY53 and an inducible phenylalanine ammonia-lyase in wheat reduces aphid resistance. Plant Biotech J, 2010, 8: 1023–1032



[8]Wang G F, Wei X N, Fan R C, Zhou H B, Wang X P, Yu C M, Dong L L, Dong Z Y, Wang X J, Kang Z S, Ling H Q, Shen Q H, Wang D W, Zhang X Q. Molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (Hsp90): functional involvement of cytosolic Hsp90s in the control of wheat seedling growth and disease resistance. New Phytol, 2011, 191: 418–431



[9]Bennypaul H S, Mutti J S, Rustgi S, Kumar N, Okubara P A, Gill K S. Virus-induced gene silencing (VIGS) of genes expressed in root, leaf, and meiotic tissues of wheat. Funct Integr Genomics, 2012, 12: 143–156



[10]Campbell J, Huang L. Silencing of multiple genes in wheat using barley stripe mosaic virus. J Biotech Res, 2010, 2: 12–20



[11]王肃威, 许长成, 白克智, 张其德, 李良璧, 匡廷云, 李继云, 李振声. 两个不同基因型小麦光抑制特性的比较. 植物学报, 2000, 42: 1300–1303



    Wang S W, Xu C C, Bai K Z, Zhang Q D, Li L B, Kuang T Y, Li J Y, Li Z S. Comparative study on photoinhibition between two wheat genotypes. Acta Bot Sin, 2000, 42: 1300–1303 (in Chinese with English abstract)



[12]Yang X H, Chen X Y, Ge Q Y, Li B, Tong Y P, Zhang A M, Li Z S, Kuang T Y, Lu C M. Tolerance of photosynthesis to photoinhibition, high temperature and drought stress in flag leaves of wheat: a comparison between a hybridization line and its parents grown under field conditions. Plant Sci, 2006, 171: 389–397



[13]Yang X H, Chen X Y, Ge Q Y, Li B, Tong Y P, Li Z S, Kuang T Y, Lu C M. Characterization of photosynthesis of flag leaves in a wheat hybrid and its parents grown under field conditions. J Plant Physiol, 2007, 164: 318–326



[14]李宏伟, 李滨, 郑琪, 李振声. 小麦幼苗从低光到强光适应过程中光合和抗氧化酶变化. 作物学报, 2010, 36: 449–456



    Li H W, Li B, Zheng Q, Li Z S. Variation in photosynthetic traits and antioxidant enzyme activities of wheat seedlings transferred from low to high light growth condition. Acta Agron Sin, 2010, 36: 449–456 (in Chinese with English abstract)



[15]Li H W, Tong Y P, Li B, Jing R L, Lu C M, Li Z S. Genetic analysis of tolerance to photo-oxidative stress induced by high light in winter wheat (Triticum aestivum L.). J Genet Genomics, 2010, 37: 399–412



[16]Loreto F, Velikova V. Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiol, 2001, 127: 1781–1787



[17]Hodges D M, DeLong J M, Forney C F, Prange R K. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 1999, 207: 604–611



[18]陈建明, 俞晓平, 程家安. 叶绿素荧光动力学及其在植物抗逆生理研究中的应用. 浙江农业学报, 2006, 18(1): 51–55



    Chen J M, Yu X P, Cheng J A. The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Acta Agric Zhejiangensis, 2006, 18(1): 51–55 (in Chinese with English abstract)



[19]Appenroth K J, Stöckel J, Srivastava A, Strasser R J. Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements. Environ Pollut, 2001, 115: 49–64



[20]Van Heerden P D R, Tsimilli-Michael M, Krüger G H J, Strasser R J. Dark chilling effects on soybean genotypes during vegetative development: parallel studies of CO2 assimilation, chlorophyll a fluorescence kinetics O-J-I-P and nitrogen fixation. Physiol Plant, 2003, 117: 476–491



[21]Krishnaswamy S, Verma S, Rahman M H, Kav N N V. Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis. Plant Mol Biol, 2011, 75: 107–127



[22]Zhang G Y, Chen M, Li L C, Xu Z S, Chen X P, Guo J M, Ma Y Z. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. J Exp Bot, 2009, 60: 3781–3796



[23]Asahina M, Azuma K, Pitaksaringkarn W, Yamazaki T, Mitsuda N, Ohme-Takagi M, Yamaguchi S, Kamiya Y, Okada K, Nishimura T, Koshiba T, Yokota T, Kamada H, Satoh S. Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in Arabidopsis. Proc Natl Acad Sci USA, 2011, 108: 16128–16132



[24]Jakoby M, Weisshaar B, Dröge-Laser W, Vicente-Carbajosa J, Tiedemann J, Kroj T, Parcy F. bZIP transcription factors in Arabidopsis. Trends Plant Sci, 2002, 7: 106–111



[25]Wellmer F, Kircher S, Rügner A, Frohnmeyer H, Schäfer E, Harter K. Phosphorylation of the parsley bZIP transcription factor CPRF2 is regulated by light. J Biol Chem, 1999, 274: 29476–29482



[26]Strathmann A, Kuhlmann M, Heinekamp T, Dröge-Laser W. BZI-1 specifically heterodimerises with the tobacco bZIP transcription factors BZI-2, BZI-3/TBZF and BZI-4, and is functionally involved in flower development. Plant J, 2001, 28: 397–408



[27]Chen R M, Ni Z F, Nie X L, Qin Y X, Dong G Q, Sun Q X. Isolation and characterization of genes encoding Myb transcription factor in wheat (Triticum aestivem L.). Plant Sci, 2005, 169: 1146–1154



[28]Lee T G, Jang C S, Kim J Y, Kim D S, Park J H, Kim D Y, Seo Y W. A Myb transcription factor (TaMyb1) from wheat roots is expressed during hypoxia: roles in response to the oxygen concentration in root environment and abiotic stresses. Physiol Plant, 2007, 129: 375–385



[29]杨文杰, 杜海, 方芳, 杨婉身, 吴燕民, 唐益雄. 大豆两个MYB转录因子基因的克隆及表达分析. 中国农业科学, 2008, 41: 961–970



    Yang W J, Du H, Fang F, Yang W S, Wu Y M, Tang Y X. Cloning and characterization of two new MYB transcription factor genes from soybean. Sci Agric Sin, 2008, 41: 961–970 (in Chinese with English abstract)



[30]郭华军, 焦远年, 邸超, 姚冬霞, 张盖华, 郑雪, 刘岚, 张群莲, 郭蔼光, 苏震. 拟南芥转录因子GRAS家族基因群响应渗透和干旱胁迫的初步探索. 植物学报, 2009, 44: 290–299



    Guo H J, Jiao Y N, Di C, Yao D X, Zhang G H, Zheng X, Liu L, Zhang Q L, Guo A G, Su Z. Discovery of Arabidopsis GRAS family genes in response to osmotic and drought stresses. Chin Bull Bot, 2009, 44: 290–299 (in Chinese with English abstract)



[31]Czikkel B E, Maxwell D P. NtGRAS1, a novel stress-induced member of the GRAS family in tobacco, localizes to the nucleus. J Plant Physiol, 2007, 164: 1220–1230
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