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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1215-1223.doi: 10.3724/SP.J.1006.2016.01215

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Plant Growth Regulators on Key Enzymes in Sucrose Metabolism of Ear Leaf and Grain at Filling Stage of Spring Maize

LI Guang-Yan,WANG Qing-Yan,XU Yan-Li,LU Lin,Jiao Liu,DONG Xue-Rui,DONG Zhi-Qiang   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Cultivation, Beijing 100081, China
  • Received:2015-12-31 Revised:2016-05-09 Online:2016-08-12 Published:2016-05-30
  • Contact: 董志强, E-mail: dongzhiqiang@caas.cn, Tel: 010-82106043 E-mail:liguangyan5112@126.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31470087), the National Key Technology R&D Program of China (2012BAD04B01-07, 2012BAD4B02) and Science and Technology InnovationProject of Chinese Academy of Agricultural Sciences

Abstract:

In order to explore the new cultivation techniques, chemical control was established inthis study.We conducted a field experiment using two varieties of Zhongdan 909 and Jidan 35with treatments of leaf-sprayingethylene-chlormequat-potassium (ECK) in V6, andPASP-KT-NAA (PKN) in V9. The results showed that ECK & PKN increased the activities of sucrose synthetase(SS)and sucrose phosphate synthetase (SPS) and sucrose content during early grain filling stage (10-20 d after anthesis)in ear leaf, and decreased sucrose content in ear leaf during late grain filling stages (30-50 d after anthesis),especiallyit improved the ability of sucrose synthesis at the late filling stage (50 d after anthesis). ECK & PKN also decreased SS (synthesis) activity in grain during middle and late grain filling stages and increased SS (resolving) activity, promoting sucrose decomposition ability, withless effect on SS(synthesis) and SPS activitiesin grain. The yields of Zhongdan 909 and Jidan 35 compared with their own control were respectively increased by 4.61% and 7.78%.The studyindicated that ECK & PKN enhance sucrose synthesis in leaf and decompositionability in grain, andpromote the translocation of carbon assimilation to grain, resulting in highgrain-filling rate,high grain weight, and high yield.

Key words: Spring maize, ECK, PKN, Sucrose, Key enzymes of sucrose metabolism

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