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作物学报 ›› 2009, Vol. 35 ›› Issue (12): 2304-2308.doi: 10.3724/SP.J.1006.2009.02304

• 研究简报 • 上一篇    

两个水稻叶色突变体的光合特性研究

吕典华,宗学凤*,王三根*,凌英华,桑贤春,何光华   

  1. 西南大学农学与生物科技学院/农业西南作物遗传改良与育种重点开放实验室，重庆400716
  • 收稿日期:2009-03-25 修回日期:2009-06-25 出版日期:2009-12-10 网络出版日期:2009-09-10
  • 通讯作者: 宗学凤, E-mail: zxfeng@swu.edu.cn, spirts2005@126.com; 王三根, E-mail: wangsg@swu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目（30800598）和重庆市水稻玉米良种创新重大专项（CSTC，2007AB1033；2007AA1019，2009BA1006）资助。

Characteristics of Photosynthesis in Two Leaf Color Mutants of Rice

LÜ Dian-Hua,ZONG Xue-Feng*,WANG San-Gen*,LING Ying-Hua,SANG Xian-Chun,HE Guang-Hua   

  1. College of Agronomy and Biotechnology,Southewst University/Key Laboratory of Southwest Crop Genetic Improvement and Breeding,Ministry of Agriculture,Chongqing 400716,China
  • Received:2009-03-25 Revised:2009-06-25 Published:2009-12-10 Published online:2009-09-10
  • Contact: ZONG Xue-Feng, E-mail: zxfeng@swu.edu.cn, spirts2005@126.com; WANG San-Gen, E-mail: wangsg@swu.edu.cn

PDF

3034

被引次数

122

摘要：

以恢复系缙恢10号为对照，对它的两个叶色突变体ygl5和pygl1在孕穗期的光合、荧光特性和叶绿体超微结构进行了研究。结果表明，两个突变体叶绿体内基粒数量明显少于对照，叶绿素含量也大幅下降，其中ygl5表现为叶绿素总体缺乏，pygl1表现为叶绿素b严重缺乏；ygl5和pygl1的Pn、光饱和点、光补偿点、暗呼吸速率、表观量子效率和羧化效率都显著高于对照，而CO2饱和点、CO2补偿点和光呼吸速率则低于对照；其Fv/Fm、ΦPSII和qP均显著高于对照，表明突变体ygl5和pygl1具有较高的光能捕获效率和转换效率。

关键词: 水稻, 叶色突变体, 光合速率, 叶绿素荧光, 叶绿体超微结构

Abstract:

Two leaf color mutants, ygl5 and pygl1, were identified from the progeny of Jinhui10 treated with EMS, and Jinhui10 was an excellent restorer line bred in the Rice Research Institute of Southwest University. This paper reported their photosynthetic parameters and ultra-structure of chloroplast. Compared with the original parent, both mutants had a decline content of chlorophylls significantly, especially in total chlorophylls for ygl5 and in chlorophyll b for the pygl1. Photosynthetic and chlorophyll fluorescence parameters were determined by LI-6400, the results showed that the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) were higher in the mutants, with higher light compensation point (LCP) and lower light saturation point (LSP) as well as lower CO2 compensation point (CCP) and CO2 saturation point (CSP). Compared with Jinhui 10, the mutants displayed less chloroplast grana and higher optional maximal photochemical efficiency of PSII (Fv/Fm), actual photochemical efficiency of PSII (ΦPSII), and photochemical quenching (qP), suggesting that the mutants have higher light energy capture and conversion efficiencies as well as the electron transport efficiency.

Key words: Oryza sativa L., Leaf color mutants, Photosynthesis rate, Chlorophyll fluorescence, Chloroplast ultra-structure


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