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作物学报 ›› 2012, Vol. 38 ›› Issue (11): 2015-2023.doi: 10.3724/SP.J.1006.2012.02015

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜隐性核不育系20118A的育性遗传及分子标记辅助选择

任梦阳1,2，倪西源2，王灏3，陈飞2，田建华3，黄吉祥2，李殿荣3，赵坚义2,*   

  1. 1 浙江师范大学化学与生命科学学院, 浙江金华 321000; 2 浙江农业科学院作物与核技术利用研究所, 浙江杭州 310021; 3 陕西省杂交油菜研究中心, 陕西大荔 715105
  • 收稿日期:2012-02-11 修回日期:2012-04-16 出版日期:2012-11-12 网络出版日期:2012-05-11
  • 通讯作者: 赵坚义, E-mail: jyzhao3@yahoo.com, Tel: 0571-86403406
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA10A104)和浙江省科技厅重大专项(2011C12005)资助。

Inheritance of Sterility in Genic Male Sterile Line (20118A) and Marker-Assisted Selection in Hybrid Breeding of Brassica napus L.

REN Meng-Yang1,2,NI Xi-Yuan2,WANG Hao3,CHEN Fei2,TIAN Jian-Hua3,HUANG Ji-Xiang2,LI Dian-Rong3,ZHAO Jian-Yi2,*   

  1. 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, China
  • Received:2012-02-11 Revised:2012-04-16 Published:2012-11-12 Published online:2012-05-11
  • Contact: 赵坚义, E-mail: jyzhao3@yahoo.com, Tel: 0571-86403406

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1309

被引次数

12

摘要：

以20118A不育系和临保系与22个油菜品种(系)杂交测交后代为材料, 采用经典遗传学和分子标记辅助选择方法, 验证该不育系统遗传控制体系及等位基因分布频率; 探索利用连锁共显性标记筛选两型系和临保系基因型的高效性和准确率。研究表明, 6个品种(系)与20118A测交产生的F2世代, 所有组合可育株∶不育株均符合3∶1或13∶3分离规律, 而与20118A-TAM杂交产生的6个F2中1个呈13∶3分离, 其余均为全可育, 育性符合1对隐性不育基因和1对隐性上位抑制基因互作控制的遗传模式; 进而采取反向验证方法, 从1 059个F2分离单株中, 用新发展的Bnms3/Bnrf连锁共显性标记跟踪选择, 直接获得临保系(ms3ms3rfrf)、纯合不育株(ms3ms3RfRf)和两型系可育株(Ms3ms3RfRf) 70、69和135株, 经测交或互交验证, 准确率均达95%以上; 根据20个测交品种的后代分离, BnRf位点上只出现Rf和rf两个等位基因, 推测第3个等位基因存在的频率很低, 基本可以根据两基因各2等位基因互作原理开展分子标记辅助育种。

关键词: 甘蓝型油菜, 隐性上位核不育, 分子标记辅助选育, 两型系, 临保系

Abstract:

In the present study, twenty varieties/lines of Brassica napus were test-crossed with sterile lines 20118A, and its temporary maintainer 20118A-TAM. Both traditional genetic analysis and molecular marker assisted technology were employed to confirm their genetic model of sterility with two gene control system, to check the allelic distributions among normal rapeseed varieties or lines. Furthermore, the efficiency and accuracy of marker-assisted selection (MAS) for two-type line and temporary maintainers based on co-dominant markers were also investigated. The results showed that the segregation proportion of male fertile to sterile plants in F2 progenies from six varieties (lines) crossed with 20118A fitted mendelian segregation (3:1 and 13:3), and that with 20118A-TAM showed either 13:3 or full male fertile, indicating that the sterility of 20118Ais controlled by one recessive sterile gene interacting with a recessive epistatic suppression gene. In addition, a reverse validation approach based on Bnms3 and Bnrf linked marker assisted selection was used to further confirm the two gene control system. From a total of 1059 F2 plants, 70, 69 and 135 individuals carrying temporary maintainer(ms3ms3rfrf), homozygous sterile (ms3ms3RfRf) and fertile (Ms3ms3RfRf) marker genotypes were screened out, respectively, which also fitted the Mendelian segregation proportions of two gene model (1/16, 1/16, and 1/8). After test-crossing with known homozygous sterile plants or temporary maintainers or one another among marker genotypes, higher than 95% of lines were approved to be the expected genotypes. Finally, according to the information from 20 testcross cultivars/lines, only two alleles of Rf and rf were observed in BnRf locus, implying the third allele naturally existed very few, if any. Therefore, for practical breeding purpose, a marker assisted selection strategy simply based on BnRf/rf and BnMs3/ms3linked co-dominant markers is proposed.

Key words: Brassica napus L., Recessive genic male sterility, Molecular marker assisted selection, Two-type line, Temporary maintainer

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