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作物学报 ›› 2015, Vol. 41 ›› Issue (06): 963-971.doi: 10.3724/SP.J.1006.2015.00963

• 研究简报 • 上一篇    下一篇

普通菜豆品种苗期抗旱性鉴定

李龙,王兰芬,武晶,景蕊莲,王述民   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京 100081
  • 收稿日期:2014-12-04 修回日期:2015-04-02 出版日期:2015-06-12 网络出版日期:2015-04-07
  • 通讯作者: 王述民, E-mail: wangshumin@caas.cn, Tel: 010-62175628
  • 基金资助:

    本研究由中国农业科学院科技创新工程和国家现代农业产业技术体系建设专项(CARS-09)资助。

Identification of Drought Resistence at Seedlings Stage in Common Bean (Phaseolus vulgaris L.) Varieties

LI Long,WANG Lan-Fen,WU Jing,JING Rui-Lian,WANG Shu-Min*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2014-12-04 Revised:2015-04-02 Published:2015-06-12 Published online:2015-04-07
  • Contact: 王述民, E-mail: wangshumin@caas.cn, Tel: 010-62175628

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摘要：

以不同来源的普通菜豆品种，采用盆栽法，设正常供水和反复干旱2种处理，测定11项生理指标，采用灰色关联度理论进行苗期抗旱性指标筛选，通过加权抗旱指数(weighted drought-resistance index, WDI值)和抗旱度量值D (drought resistance comprehensive evaluation values, D值) 对供试材料进行抗旱性综合评价并通过聚类分析划分抗旱等级。结果表明，不同指标与综合抗旱指数的关联度大小依次为叶片相对含水量(0.7726)、PSII最大量子产量(0.7607)、叶绿素含量(0.7435)、反复干旱存活率(0.7341)、生物量(0.7329)、茎叶干重(0.7314)、根干重(0.7192)、气孔导度(0.7159)、根冠比(0.7092)、株高(0.7086)、叶面积(0.6910)。基于加权抗旱指数和抗旱度量值D的评价结果存在一定差异，但不同材料的抗旱性排序大体一致。根据抗旱度量值D将供试材料分为高抗、中抗、较敏感和敏感4个级别，各占总数的10%、6%、58%和26%。综上所述，叶片相对含水量、PSII最大量子产量和叶绿素含量等10项指标可用于普通菜豆苗期抗旱性综合评价；加权抗旱指数与抗旱度量值D两种综合指标相结合能够提高鉴定结果的可靠性；50个参试普通菜豆品种中，白金德利豆、跃进豆、兔子腿、圆白菜豆和260205抗旱性强。

关键词: 普通菜豆, 抗旱性, 灰色关联度分析, 模糊聚类分析, 综合评价

Abstract:

Eleven physiological indices of different common bean varieties were measured under the conditions of irrigation and repeated drought in pot, drought resistance evaluation indices at seedling stage were selected by using grey relational thoery, the drought resistance of each germplasm was comprehensively evaluated according to weighted drought-resistance index and drought-resistance measuration value (D value) and graded by fuzzy cluster analysis. The results showed that the association degree between different indices and comprehensive drought resistance indices was ranked as follows: leaf relative water content (0.7726), Fv/Fm (0.7607), chlorophyll content (0.7435), survival rate (0.7341), biomass (0.7329), shoot dry weight (0.7314), root dry weight (0.7192), stomatal conductance (0.7159), Root/Shoot ratio (0.7092), plant height (0.7086), leaf area (0.6910). The evaluation results based on weighted drought-resistance index and D value had some differences, but the drought resistance rank was essentially the same. According to D value, tested materials were divided into four groups including high resistance(10%), moderate resistance(6%), susceptible(58%) and high susceptible(26%). In conclusion, ten indices including leaf relative water content, Fv/Fm, chlorophyll content and so on can be used to evaluate the drought resistance at seedling stage in common bean; combining weighted drought-resistance index and D value can improve the reliability of identification results. Among the 50 tested varieties, Baijindelidou, Yuejindou, Tuzutui, Yuanbaicaidou and 260205 have the better drought resistance.

Key words: Common bean, Drought resistance, Grey relational analysis, Fuzzy cluster analysis, Comprehensive evaluation

[1]Beebe S E, Rao I M, Blair M W, Acosta-Gallegos J A. Phenotyping common beans for adaptation to drought. Front Physiol, 2013, 4: 35



[2]FAOSTAT. Statistics Database [January 2015]. Rome Available at: http://faostat.fao.org/



[3]Rosales M A, Ocampo E, Rodríguez-Valentín R, Olvera-Carrillo Y, Acosta-Gallegos J, Covarrubias A A. Physiological analysis of common bean (Phaseolus vulgaris L.) cultivars uncovers characteristics related to terminal drought resistance. Plant Physiol Bioch, 2012, 56: 24–34



[4]White J W, Castillo J A. Studies at CIAT on mechanisms of drought tolerance in bean. In: White J W, Castillo J A, eds. Research on Drought Tolerance in Common Bean. Colombia: CIAT Press, 1988. pp 146–164



[5]Rao I M, Beebe S, Polania J. Evaluation of drought resistance and associated traits in advanced lines. In: Rao I M, Beebe S, eds. Bean Improvement for the Tropics. Colombia: CIAT Press, 2004. pp 5–13



[6]Rao I M, Beebe S, Polania J, Grajales M A, Garcia R. Differences in drought resistance of advanced lines developed for the last 3 decades. In: Grajales M A, Garcia R, eds. Bean Improvement for the Tropics. Colombia: CIAT Press, 2006. pp 2–6



[7]畅建武. 粒用菜豆种质资源芽期抗旱性研究. 山西农业科学, 1996, 24: 31–34



Chang J W. Studies on seedling drought resistance of common bean. J Shanxi Agric Sci, 1996, 24:31–34 (in Chinese with English abstract)



[8]孟庆立, 关周博, 冯佰利, 柴岩, 胡银岗. 谷子抗旱相关性状的主成分与模糊聚类分析. 中国农业科学, 2009, 42: 2667–2675



Meng Q L, Guan Z B, Feng B L, Cai Y, Hu Y G. Principal component analysis and fuzzy clustering on drought-tolerance related traits of foxtail millet (Setaria italica). Sci Agric Sin, 2009, 42: 2667–2675 (in Chinese with English abstract)



[9]赖运平, 李俊, 张泽全, 董雪芳, 刘新春, 魏会廷, 胡晓蓉, 彭正松, 杨武云. 小麦苗期抗旱相关形态指标的灰色关联度分析. 麦类作物学报, 2009, 29: 1055–1059



Lai Y P, Li J, Zhang Z Q, Dong X F, Liu X C, Wei H T, Hu X R, Peng Z S, Yang W Y. Grey correlation analysis of morphological traits related to drought tolerance of wheat at seedling stage. J Triticeae Crops, 2009, 29: 1055–1059 (in Chinese with English abstract)



[10]谢小玉, 张霞, 张兵. 油菜苗期抗旱性评价及抗旱相关指标变化分析. 中国农业科学, 2013, 46: 476–485



Xie X Y, Zhang X, Zhang B. Evaluation of drought resistance and analysis of variation of relevant parameters at seedling stage of rapeseed (Brassica napus L.). Sci Agric Sin, 2013, 46: 476–485 (in Chinese with English abstract)



[11]王述民, 张亚芝, 魏淑红. 普通菜豆种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006. pp 50–52



Wang S M, Zhang Y Z, Wei S H. Descriptors and Data Standardfor Common Bean (Phaseolus vulgaris L.). Beijing: China Agriculture Press, 2006. pp 50–52 (in Chinese)



[12]李龙, 王兰芬, 武晶, 王述民. 普通菜豆种质资源芽期抗旱性鉴定. 植物遗传资源学报, 2013, 14: 600–605



Li L, Wang L F, Wu J, Wang S M. Drought resistance identification of common bean germplasm resources at bud stage. J Plant Genet Res, 2013, 14: 600–605 (in Chinese with English abstract)



[13]程芳艳, 王继亮, 刘海云, 王敏, 杜维俊. 野生和地方大豆品种耐旱性初步评价. 山西农业科学, 2007, (7): 31–35



Cheng F Y, Wang J L, Liu Y H, Wang M, Du W J. Identification of drought tolerance in wild soybeans and local cultivars. J Shanxi Agric Sci, 2007, (7): 31–35 (in Chinese with English abstract)



[14]Ouelhadj A, Kuschk P, Humbeck K. Heavy metal stress and leaf senescence induce the barley gene HvC2d1 encoding a calcium-dependent novel C2 domain-like protein. New Phytol, 2006, 170: 261–273



[15]郭志利, 卢成达, 李阳, 张丽娜, 韵晓冬, 孙迪. 谷子不同品种、生育时期、叶位光合特性研究. 农学学报, 2014, (4): 5–9



Guo Z L, Lu C D, Li Y, Zhang L N, Yun X D, Sun D. Photosynthetic characteristics research on different varieties, growth period, the whole plant different leaf of millet. J Agric, 2014, (4): 5–9 (in Chinese with English abstract)



[16]唐钢梁, 李向义, 林丽莎, 李磊, 鲁建荣. 骆驼刺在不同遮阴下的水分状况变化及其生理响应. 植物生态学报, 2013, 37: 354–364



Tang G L, Li X Y, Lin L S, Li L, Lu J R. Change of different shading on moisture conditions and the physiological response in Alhagi sparsifolia. Acta Phytoecol Sin, 2013, 37: 354–364 (in Chinese with English abstract)



[17]耿摇婷, 陈金, 张卫建, 陈长青. 全天增温对不同年代冬小麦品种生长发育及产量的影响. 应用生态学报, 2014, 25: 1357–1365



Geng Y T, Chen J, Zhang W J, Chen C Q. Effects of all-day warming on growth, development and yield of winter wheat cultivars bred in different decades. Acta Phytoecol Sin, 2014, 25: 1357–1365 (in Chinese with English abstract)



[18]鲁守平, 孙群, 洪露, 王建华, 孙宝启. 不同种源地乌拉尔甘草发芽期抗旱性鉴定. 植物遗传资源学报, 2007, 8: 189–194



Lu S P, Sun Q, Hong L, Wang J H, Sun B Q. Drought resistance evaluation of Glyccyrrhiza uralensis Fisch from different regions in germination stage. J Plant Genet Resourc, 2007, 8: 189–194 (in Chinese with English abstract)



[19]兰巨生. 农作物综合抗旱性评价方法的研究. 西北农业学报, 1998, 7: 85–87



Lan J S. Comparison of evaluating methods for agronomic drought resistance in crops. Acta Agric Boreali-occident Sin, 1998, 7: 85–87 (in Chinese with English abstract)



[20]祁旭升, 王兴荣, 许军, 张建平, 米君. 胡麻种质资源成株期抗旱性评价. 中国农业科学, 2010, 43: 3076–3087



Qi X S, Wang X R, Xu J, Zhang J P, Mi J. Drought-resistance evaluation of flax germplasm at adult plant stage. Sci Agric Sin, 2010, 43: 3076–3087 (in Chinese with English abstract)



[21]尹利, 逯晓萍, 傅晓峰, 李美娜, 郭建. 高丹草杂交种灰色关联分析与评判. 中国草地学报, 2006, 28: 21–25



Yin L, Lu X P, Fu X F, Li M N, Guo J. The grey relation analysis and evaluation of hybrid pacesetter. Chin J Grassland, 2006, 28: 21–25 (in Chinese with English abstract)



[22]申卯兴, 薛西锋, 张小水. 灰色关联分析中分辨系数的选取. 空军工程大学学报, 2003, (4): 68–70



Shen M X, Xue X F, Zhang X S. Determination of discrimination coefficient in grey incidence analysis. J Air Force Engin Univ, 2003, (4): 68–70 (in Chinese with English abstract)



[23]王士强, 胡银岗, 佘奎军, 周琳璘, 孟凡磊. 小麦抗旱相关农艺性状和生理生化性状的灰色关联度分析. 中国农业科学, 2007, 40: 2452–2459



Wang S Q, Hu Y G, She K J, Zhou L L, Meng F L. Gray relational grade analysis of agronomical and physi-biochemical traits related to drought tolerance in wheat. Sci Agric Sin, 2007, 40: 2452–2459 (in Chinese with English abstract)



[24]Munoz-Perea C G, Allen R G, Westermann D T, Wright J L, Singh S P. Water use efficiency among dry bean landraces and cultivars in drought-stressed and non-stressed environments. Euphytica, 2007, 155: 393–402



[25]Mohamed M F, Schmitz-Eiberger N, Keutgen N, Noga G. Comparative drought postponing and tolerance potentials of two tepary bean lines in relation to seed yield. Afr Crop Sci J, 2005, 13: 49–60



[26]Wentworth M, Murchie E H, Gray J E, Villegas D, Pastenes C, Pinto M, Horton P. Differential adaptation of two varieties of common bean to abiotic stress. II: acclimation of photosynthesis. J Exp Bot, 2006, 57: 699–709



[27]Mukeshimana G, Lasley A L, Loescher W H, Kelly J D. Identification of shoot traits related to drought tolerance in common bean seedlings. J Am Soc Hort Sci, 2014, 139: 299–309



[28]王贺正, 李艳, 马均, 张荣萍, 李旭毅, 汪仁全. 水稻苗期抗旱性指标的筛选. 作物学报, 2007, 33: 1523–1529



Wang H Z, Li Y, Ma J, Zhang R P, Li X Y, Wang R Q. Screening indexes of drought resistance during seedling stage in rice. Acta Agron Sin, 2007, 33: 1523–1529 (in Chinese with English abstract)



[29]李震, 吴北京, 陆光远, 程勇, 邹崇顺, 张学昆. 不同基因型油菜对苗期水分胁迫的生理响应. 中国油料作物学报, 2012, (1): 33–39



Li Z, Wu B J, Lu G Y, Cheng Y, Zhou C S, Zhang X K. Differences in physiological responses of Brassica napus genotypes under water stress during seedling stage. Chin J Oil Crop Sci, 2012, (1): 33–39 (in Chinese with English abstract)



[30]高吉寅, 胡荣海, 路漳, 杨国良. 水稻等品种苗期抗旱生理指标的探讨. 中国农业科学, 1984, (4): 41–45



Gao J Y, Hu R H, Lu Z, Yang G L. Investigation on the physiological indices of drought resistance in the seedling stage of rice. Sci Agric Sin, 1984, (4): 41–45 (in Chinese with English abstract)



[31]李壮, 许文娟, 薛兵东, 曹萍. 玉米苗期抗旱性评定方法探讨. 玉米科学, 2004, 12(2): 73–75



Li Z, Xu W J, Xue B D, Cao P. Discuss on evaluating method to drought-resistance of maize in seedling stage. J Maize Sci, 2004, 12(2): 73–75 (in Chinese with English abstract)



[32]罗俊杰, 欧巧明, 叶春雷, 王方, 王镛臻, 陈玉梁. 重要胡麻栽培品种的抗旱性综合评价及指标筛选. 作物学报, 2014, 40: 1259–1273



Luo J J, Ou Q M, Ye C L, Wang F, Wang Y Z, Chen Y L. Comprehensive valuation of drought resistance and screening of indices of important flax cultivars. Acta Agron Sin, 2014, 40: 1259–1273 (in Chinese with English abstract)
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