作物学报 ›› 2024, Vol. 50 ›› Issue (10): 2435-2446.doi: 10.3724/SP.J.1006.2024.32060
周田田1,2(), 唐兆成1, 李笑1, 朱鹏1, 邓晶晶1, 杨郁文1, 张保龙1,2, 郭冬姝1,*()
ZHOU Tian-Tian1,2(), TANG Zhao-Cheng1, LI Xiao1, ZHU Peng1, DENG Jing-Jing1, YANG Yu-Wen1, ZHANG Bao-Long1,2, GUO Dong-Shu1,*()
摘要:
水稻(Oryza sativa L.)是重要的粮食作物,蛋白质是稻米的第二大营养物质。肾脏病人通常需要限制蛋白质的摄入量以减轻肾脏的负担和控制病情进展。稻米蛋白中谷蛋白含量最高,且易被人体消化。本研究以LGC-1作为供体亲本培育出的粳稻品种为转基因受体,利用CRISPR/Cas9介导的基因编辑技术一次性敲除谷蛋白A (Glutelin A,GluA)亚家族编码基因GluA1、GluA2和GluA3,获得了不含转基因元件的谷蛋白含量约为1.8%的低谷蛋白种质,并对所得种质的稻米品质和农艺性状进行了全面评估。所得低谷蛋白种质外观品质较好,相比于受体品种垩白度降低、糙米率和精米率提高。本研究为创制低谷蛋白水稻品种提供了一种高效、简便的方法,为培育适合肾病患者的功能性水稻品种提供了一种新的遗传材料。
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