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作物学报 ›› 2025, Vol. 51 ›› Issue (7): 1784-1800.doi: 10.3724/SP.J.1006.2025.41082

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

山西小麦醇溶蛋白多样性及其对面粉品质效应研究

王天译1,2**(), 杨绣娟3**(), 赵佳佳1, 郝宇琼1, 郑兴卫1, 武棒棒1, 李晓华1, 郝水源3, 郑军1,2,*()   

  1. 1山西农业大学小麦研究所 / 农业农村部有机旱作农业重点实验室(部省共建), 山西临汾 041000
    2山西农业大学农学院, 山西晋中 030801
    3河套学院农学系, 内蒙古巴彦淖尔 015000
  • 收稿日期:2024-11-25 接受日期:2025-04-25 出版日期:2025-07-12 网络出版日期:2025-05-09
  • 通讯作者: *郑军, E-mail: sxnkyzj@126.com
  • 作者简介:王天译, E-mail: wangtianyi0204@163.com;
    杨绣娟, E-mail: yangxiujuaned@163.com
    **同等贡献
  • 基金资助:
    山西省重点研发计划项目(202302140601001);山西省现代农业产业技术体系项目(2024CYJSTX02);山西省小麦育种联合攻关项目(NYGG26-01);山西“1331工程”作物科学一级学科建设项目资助

Gliadin diversity and its effects on flour quality in wheat from Shanxi province, China

WANG Tian-Yi1,2**(), YANG Xiu-Juan3**(), ZHAO Jia-Jia1, HAO Yu-Qiong1, ZHENG Xing-Wei1, WU Bang-Bang1, LI Xiao-Hua1, HAO Shui-Yuan3, ZHENG Jun1,2,*()   

  1. 1Institute of Wheat Research, Shanxi Agriculture University / Key Laboratory of Sustainable Dryland Agriculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Linfen 041000, Shanxi, China
    2College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
    3Department of Agronomy, Hetao College, Bayannur 015000, Inner Mongolia, China
  • Received:2024-11-25 Accepted:2025-04-25 Published:2025-07-12 Published online:2025-05-09
  • Contact: *E-mail: sxnkyzj@126.com
  • About author:**Contributed equally to this work
  • Supported by:
    Key Research and Development Project of Shanxi Province(202302140601001);Modern Agro-industry Technology Research System of Shanxi Province(2024CYJSTX02);Shanxi Joint Action Program of Wheat Breeding(NYGG26-01);Shanxi “1331 Project” Crop Science First-Class Discipline Construction Project

摘要: 山西省小麦种质资源丰富, 以抗旱性强和品质优异而著称, 但醇溶蛋白多样性研究不足。为阐明山西小麦醇溶蛋白多样性及其对面粉品质的效应, 采用酸性聚丙烯酰胺凝胶电泳鉴定了421份山西小麦的醇溶蛋白谱带组成, 分析了不同谱带对面粉品质的效应。结果表明, 421份材料醇溶蛋白多态性整体上较高, 具有91种谱带类型, 共分离出8585条谱带, 平均每个品种含有20.39条, 品种间谱带数量变异范围为15~27条, 基于遗传距离的聚类, 所有品种可分为5大类。醇溶蛋白多态性信息含量、基因多样性以及遗传距离受品种亲缘关系和生态分布的影响, 除不同麦区间的种质谱带数量存在差异外, 育成品种平均谱带数量高于地方品种, 水地品种高于旱地品种, 育成品种的谱带多样性随育种年代呈先升后降的趋势。山西小麦同名地方品种的醇溶蛋白谱带数量和多样性均有差异, 存在同名同种和同名异种的现象; 进一步发现地方品种小红皮是由多个纯系组成的混合群体, 有3种不同的醇溶蛋白单元型。相关性分析发现34条谱带与5项面粉理化性质相关, 58条谱带与12项面粉加工品质相关, 正效应谱带为15条, 其中迁移率为23.4和64.1的谱带可分别提高吸水率2.0%和2.1%, 谱带45.2可提高湿面筋含量0.9%, 14.1、43.3和65.4等13条谱带可以提高粉质质量, 其中谱带65.4的效应最高, 可以提高11.3%, 14.1、20.6和43.3等谱带可同时提高面团形成时间和稳定时间; 25条谱带与黏度特性显著相关, 呈正相关的谱带共9条, 其中谱带76.8效应最高, 可提高最低黏度、峰值黏度和最终黏度的21.7%、12.8%和20.0%, 具有多效应谱带48条, 这些谱带在今后的应用研究中潜力较大。本研究有助于深入认识山西小麦种质资源遗传及演化, 可以为小麦品质定向改良育种提供有价值的信息。

关键词: 山西小麦, 醇溶蛋白, 遗传多样性, 地方品种, 品质性状

Abstract:

Shanxi province possesses abundant wheat germplasm resources, well known for their strong drought resistance and excellent quality. However, the diversity of gliadins in these resources has not been thoroughly investigated. To address this, acid-polyacrylamide gel electrophoresis (A-PAGE) was employed to characterize gliadin band compositions in 421 wheat accessions from Shanxi, and to assess the impact of different gliadin bands on flour quality. The results revealed high overall gliadin polymorphism, with 91 distinct band types and a total of 8585 bands identified, averaging 20.39 bands per accession. The number of bands per cultivar ranged from 15 to 27. Cluster analysis based on genetic distance grouped all accessions into five distinct categories. Gliadin polymorphism information content, genetic diversity, and genetic distance were influenced by the genetic relationships and ecological distribution of the germplasm. Notably, cultivars exhibited greater diversity than landraces, and irrigated cultivars showed higher diversity than those from dryland cultivars. The diversity of cultivars initially increased with breeding year, followed by a decline. Interestingly, landraces with the same name displayed variation in both band number and diversity, indicating the coexistence of homogeneous and heterogeneous forms. The widely cultivated landrace Xiaohongpi was found to be a mixed population composed of multiple pure lines, characterized by three distinct gliadin haplotype blocks. Correlation analysis identified 34 bands associated with five physicochemical flour properties, while 58 bands were linked to 12 flour processing quality traits, among which 15 bands had positive effects. Specifically, bands 23.4 and 64.1 increased water absorption by 2.0% and 2.1%, respectively, and band 45.2 enhanced wet gluten content by 0.9%. Moreover, bands 14.1, 43.3, and 65.4 improved farinograph quality, with band 65.4 having the strongest effect—an 11.3% increase. Bands 14.1, 20.6, and 43.3 also contributed to longer dough development and stability times. In addition, 25 bands were significantly associated with viscosity characteristics, with nine showing positive correlations. Among them, band 76.8 had the most pronounced effect, increasing minimum, peak, and final viscosity by 21.7%, 12.8%, and 20.0%, respectively. In total, 48 multi-effect bands were identified, demonstrating substantial potential for future applied research. These findings offer valuable insights into the inheritance and evolution of wheat germplasm in Shanxi, and provide a scientific basis for breeding programs aimed at improving wheat quality.

Key words: Shanxi wheat, gliadin, genetic diversity, landrace, quality trait

附表1

山西小麦品种名称及醇溶蛋白谱带数量"

序号
No.
品种
Variety
谱带数
Bands
序号
No.
品种
Variety
谱带数
Bands
L1 大白麦 Dabaimai 18 C61 运引1号 Yunyin 1 21
L2 小红皮 Xiaohongpi 19 C62 晋农207 Jinnong 207 19
L3 牛趾甲(离石) Niuzhijia (Lishi) 20 C63 运麦1934 Yunmai 1934 24
L4 红粒麦 Honglimai 21 C64 长6878 Chang 6878 21
L5 绛州红 Jiangzhouhong 18 C65 晋太170 Jintai 170 19
L6 假红麦 Jiahongmai 19 C66 临优145 Linyou 145 22
L7 牛趾甲(中阳) Niuzhijia (Zhongyang) 18 C67 临汾138 Linfen 138 22
L8 四月黄 Siyuehuang 19 C68 长6154 Chang 6154 21
L9 三月黄 Sanyuehuang 19 C69 河东TX-006 Hedong TX-006 23
L10 白芒糙 Baimangcao 20 C70 临丰3号 Linfeng 3 22
L11 有芒大红茎 Youmangdahongjing 16 C71 晋太65 Jintai 65 24
L12 竹秤青 Zhuchengqing 20 C72 临远3158 Linyuan 3158 24
L13 红秃麦(屯留) Hongtumai (Tunliu) 20 C73 临抗11号 Linkang 11 23
L14 白秃麦(屯留) Baitumai (Tunliu) 17 C74 泽优2号 Zeyou 2 23
L15 白秃麦(吉县) Baitumai (Jixian) 18 C75 冬黑1号 Donghei 1 21
L16 红秃麦(吉县) Hongtumai (Jixian) 22 C76 运黑28号 Yunhei 28 24
L17 叶蛐麦 Yequmai 18 C77 冬黑10号 Donghei 10 20
L18 红皮冬麦 Hongpidongmai 16 C78 临优2018 Linyou 2018 27
L19 白山疙瘩 Baishangeda 17 C79 临优2069 Linyou 2069 25
L20 红叶蛐 Hongyequ 19 C80 晋春3号 Jinchun 3 22
L21 气死风 Qisifeng 18 C81 晋春13 Jinchun 13 21
L22 红和尚 Hongheshang 21 C82 晋春15号 Jinchun 15 18
L23 西安麦 Xianmai 17 C83 运旱2335 Yunhan 2335 21
L24 喯麦 Penmai 20 C84 长6359 Chang 6359 21
L25 白壳红 Baikehong 18 C85 长麦5079 Changmai 5079 26
L26 红小麦 Hongxiaomai 22 C86 长6452 Chang 6452 23
L27 金裹银 Jinguoyin 21 C87 长4640 Chang 4640 22
L28 游白兰 Youbailan 20 C88 汾黑麦1831 Fenheimai 1831 22
L29 白线麦 Baixianmai 20 C89 晋麦78号 Jinmai 78 23
L30 白梗麦 Baigengmai 22 C90 晋麦79号 Jinmai 79 26
L31 白火麦 Baihuomai 21 C91 晋麦80号 Jinmai 80 21
L32 白三月黄 Baisanyuehuang 20 C92 晋麦81号 Jinmai 81 21
L33 玉兰麦 Yulanmai 18 C93 汾4846 Fen 4846 22
L34 紫麦 Zimai 20 C94 汾4439 Fen 4439 22
L35 大同小麦 Datongxiaomai 20 C95 晋麦82号 Jinmai 82 21
L36 齐穗麦 Qisuimai 17 C96 临汾8050 Linfen 8050 24
L37 大红麦 Dahongmai 17 C97 晋麦83号 Jinmai 83 22
L38 火烧头 Huostou 17 C98 临汾6510 Linfen 6510 24
L39 定兴寨小麦 Dingxingzhaixiaomai 19 C99 运旱20410 Yunhan 20410 25
L40 白山麦 Baishanmai 21 C100 长麦6686 Changmai 6686 22
L41 大灰麦 Dahuimai 21 C101 长7016 Chang 7016 26
L42 白山疙塔 Baishangeta 19 C102 晋麦84号 Jinmai 84 18
L43 土麦 Tumai 20 C103 晋麦85号 Jinmai 85 21
L44 白芒糙 Baimangcao 22 C104 晋太9923 Jintai 9923 22
L45 红秃麦(长治) Hongtumai (Changzhi) 18 C105 晋麦86号 Jinmai 86 18
L46 疙绉麦 Gezhoumai 19 C106 太5902 Tai 5902 18
L47 红壳红 Hongkehong 17 C107 长麦6135 Changmai 6135 24
L48 豹麦 Baomai 21 C108 晋麦87号 Jinmai 87 19
L49 红碧 Hongbi 20 C109 运旱719 Yunhan 719 17
L50 和尚头白宿麦 Heshangtoubaisumai 18 C110 晋麦88号 Jinmai 88 24
L51 高阳白麦 Gaoyangbaimai 23 C111 山农129 Shannong 129 22
L52 古城营 Guchengying 18 C112 太13606 Tai 13606 25
L53 白火麦 Baihuomai 17 C113 长麦5973 Changmai 5973 21
L54 杨中红山麦 Yangzhonghongshanmai 18 C114 长5222 Chang 5222 26
L55 泉社红火麦 Quanshehonghuomai 20 C115 长6388 Chang 6388 22
L56 杨中白山疙瘩 Yangzhongbaishangeda 18 C116 临远8号 Linyuan 8 23
L57 红疙瘩麦 Honggedamai 22 C117 长麦251 Changmai 251 23
L58 水麦 Shuimai 20 C118 长6197 Chang 6197 22
L59 红线麦 Hongxianmai 17 C119 长8744 Chang 8744 20
L60 小秃麦 Xiaotumai 17 C120 晋麦90号 Jinmai 90 22
L61 白芒红 Baimanghong 18 C121 晋麦91号 Jinmai 91 21
L62 白壳白 Baikebai 17 C122 运旱805 Yunhan 805 21
L63 红芒糙 Hongmangcao 18 C123 晋麦92号 Jinmai 92 18
L64 和尚头(太谷) Heshangtou (Taigu) 19 C124 晋太182 Jintai 182 22
L65 日本小麦 Ribenxiaomai 20 C125 长4853 Chang 4853 18
L66 大红袍 Dahongpao 17 C126 晋麦94号 Jinmai 94 21
L67 有芒灯笼红 Youmangdenglonghong 18 C127 晋麦95号 Jinmai 95 22
L68 紫梗茬 Zigengcha 19 C128 晋麦96号 Jinmai 96 20
L69 小红皮 Xiaohongpi 18 C129 晋太102 Jintai 102 22
L70 小红麦 Xiaohongmai 18 C130 晋麦97号 Jinmai 97 18
L71 宿麦 Sumai 20 C131 晋麦98号 Jinmai 98 19
L72 和尚头(左权) Heshangtou (Zuoquan) 20 C132 太春3473 Taichun 3473 19
L73 秃小麦 Tuxiaomai 20 C133 晋麦99号 Jinmai 99 22
L74 小芒麦 Xiaomangmai 20 C134 运旱21-30 Yunhan 21-30 20
L75 大冬小麦 Dadongxiaomai 18 C135 良星67 Liangxing 67 26
L76 白芒麦 Baimangmai 17 C136 运旱137 Yunhan 137 20
L77 竹杆青 Zhuganqing 18 C137 太113 Tai 113 18
L78 白芒红 Baimanghong 20 C138 晋作80 Jinzuo 80 18
L79 白关东 Baiguandong 16 C139 晋太114 Jintai 114 24
L80 火里烧 Huolishao 17 C140 长6794 Chang 6794 23
L81 芒麦 Mangmai 20 C141 长7080 Chang 7080 18
L82 白芒麦 Baimangmai 16 C142 晋太1310 Jintai 1310 20
L83 有芒山疙瘩 Youmangshangeda 22 C143 长6990 Chang 6990 22
L84 红碧 Hongbi 23 C144 中麦247 Zhongmai 247 18
L85 杨中三月黄 Yangzhongsanyuehuang 19 C145 临旱6号 Linhan 6 19
L86 白山麦 Baishanmai 15 C146 临Y8161 Liny 8161 20
L87 大红芒 Dahongmang 21 C147 中旱110 Zhonghan 110 21
L88 小白麦 Xiaobaimai 20 C148 吕旱1608 Lyuhan 1608 20
L89 大白麦 Dabaimai 21 C149 小黑麦76号 Xiaoheimai 76 19
L90 白线麦 Baixianmai 18 C150 鲁科298 Luke 298 22
L91 长芒白壳 Changmangbaike 23 C151 晋太141 Jintai 141 21
L92 白秃麦(石楼) Baitumai (Shilou) 21 C152 晋太146 Jintai146 22
L93 白蚂蚱麦 Baimazhamai 20 C153 太1305 Tai 1305 20
L94 四强小麦 Siqiangxiaomai 18 C154 太412 Tai 412 19
L95 大浦山麦 Dapushanmai 21 C155 运14观74 Yun 14 Guan 74 21
L96 六棱麦 Liulengmai 18 C156 尧麦16 Yaomai 16 23
L97 老白条子 Laobaitiaozi 18 C157 石农086 Shinong 086 20
L98 黄无芒老麦 Huangwumanglaomai 17 C158 翔麦23 Xiangmai 23 22
L99 四川白 Sichuanbai 19 C159 临汾6410 Linfen 6410 19
L100 四月黄 Siyuehuang 22 C160 晋麦101 Jinmai 101 19
L101 白条麦 Baitiaomai 18 C161 晋麦102 Jinmai 102 18
L102 红兰麦 Honglanmai 19 C162 晋太1515 Jintai 1515 23
L103 老条子麦 Laotiaozimai 18 C163 NC 206 NC 206 22
L104 三月黄 Sanyuehuang 17 C164 中麦175 Zhongmai 175 18
L105 山阳雪 Shanyangxue 19 C165 舜麦1718 Shunmai 1718 19
L106 百岁红里被地麦 Baisuihonglibeidimai 17 C166 运旱1512 Yunhan 1512 19
L107 老蔓麦 Laomanmai 19 C167 运旱139-2 Yunhan 139-2 19
L108 回头草 Huitoucao 23 C168 润麦2号 Runmai 2 18
L109 平遥小白麦 Pingyaoxiaobaimai 20 C169 翔麦8156 Xiangmai 8156 17
L110 牛趾甲 Niuzhijia 21 C170 临旱9号 Linhan 9 21
L111 红芒麦 Hongmangmai 22 C171 沃麦323 Womai 323 19
L112 红芒麦 Hongmangmai 22 C172 金麦919 Jinmai 919 21
L113 红和尚头 Hongheshangtou 22 C173 晋麦104 Jinmai 104 19
L114 白麦 Baimai 21 C174 长6789 Chang 6789 18
L115 红和尚头 Hongheshangtou 20 C175 临Y7287 LinY 7287 20
L116 红芒麦 Hongmangmai 23 C176 运旱1411-2 Yunhan 1411-2 19
L117 笨麦 Benmai 17 C177 晋麦9号 Jinmai 9 20
L118 白山麦 Baishanmai 17 C178 晋春14 Jinchun 14 20
L119 大红麦Dahongmai 22 C179 晋春16号 Jinchun 16 21
L120 碧玉麦 Biyumai 20 C180 晋春17号 Jinchun 17 21
L121 大白麦 Dabaimai 17 C181 运麦218 Yunmai 218 18
L122 和尚头(崞县) Heshangtou (Guoxian) 16 C182 临Y8012 LinY 8012 21
L123 和尚头(崞县) Heshangtou (Guoxian) 21 C183 沃麦608 Womai 608 23
L124 白芒麦 Baimangmai 20 C184 运黑14207 Yunhei 14207 20
L125 大白麦 Dabaimai 20 C185 运黑161 Yunhei 161 18
L126 白袍麦 Baipaomai 21 C186 运糯32 Yunnuo 32 19
L127 竹秆青 Zhuganqing 20 C187 晋麦106 Jinmai 106 18
L128 红线麦 Hongxianmai 18 C188 龙麦1号 Longmai 1 19
L129 红皮宿麦 Hongpisumai 22 C189 晋太1508 Jintai 1508 19
L130 古城营 Guchengying 20 C190 晋麦103 Jinmai 103 21
L131 小红麦 Xiaohongmai 18 C191 太冬2503 Taidong 2503 20
L132 红线麦 Hongxianmai 20 C192 长5638 Chang 5638 22
L133 无芒老麦 Wumanglaomai 19 C193 晋太1510 Jintai 1510 26
L134 大芒麦 Damangmai 20 C194 太春101 Taichun 101 24
L135 红线麦 Hongxianmai 19 C195 邯科4898 Hanke 4898 22
L136 白芒糙 Baimangcao 19 C196 运旱618 Yunhan 618 25
L137 洪洞麦 Hongdongmai 18 C197 运旱115 Yunhan 115 27
L138 和尚头(临汾) Heshangtou (Linfen) 25 C198 运旱102 Yunhan 102 25
L139 气死风 Qisifeng 19 C199 运旱22-33 Yunhan 22-33 22
L140 红乇麦 Hongtuomai 21 C200 晋麦8号 Jinmai 8 21
L141 滩麦 Tanmai 21 C201 鲁麦14 Lumai 14 18
L142 南里三月黄 Nanlisanyuehuang 21 C202 金丰3号 Jinfeng 3 20
L143 卿头双炮火麦 Qingtoushuangpaohuomai 21 C203 长麦6789 Changmai 6789 20
L144 无芒四月黄 Wumangsiyuehuang 20 C204 运9805 Yun 9805 27
L145 单炮火麦 Danpaohuomai 21 C205 临旱5325 Linhan 5325 23
L146 普乐头三月黄 Puletousanyuehuang 20 C206 长5804 Chang 5804 22
L147 有芒四月黄 Youmangsiyuehuang 20 C207 运旱1818 Yunhan 1818 21
L148 芒火麦 Manghuomai 23 C208 中麦110 Zhongmai 110 22
L149 金裹银 Jinguoyin 23 C209 中优9507 Zhongyou 9507 26
L150 早麦 Zaomai 17 C210 临汾139 Linfen 139 21
L151 杨中三月黄 Yangzhongsanyuehuang 21 C211 中麦349 Zhongmai 349 21
C1 晋麦1 Jinmai 1 22 C212 舜麦612 Shunmai 612 18
C2 晋麦5 Jinmai 5 20 C213 泽优1号 Zeyou 1 22
C3 晋麦11 Jinmai 11 20 C214 长麦3987 Changmai 3987 17
C4 晋麦12 Jinmai 12 20 C215 中优206 Zhongyou 206 22
C5 晋麦16 Jinmai 16 20 C216 JM 5295 JM 5295 20
C6 晋麦17 Jinmai 17 22 C217 晋麦100 Jinmai 100 19
C7 晋麦18 Jinmai 18 22 C218 鑫麦296 Xinmai 296 21
C8 晋麦19 Jinmai 19 23 C219 轮选167 Lunxuan 167 22
C9 晋麦20 Jinmai 20 19 C220 永麦3 Yongmai 3 20
C10 晋麦21 Jinmai 21 22 C221 翔麦517 Xiangmai 517 21
C11 晋麦22 Jinmai 22 20 C222 忻6160 Xin 6160 21
C12 晋麦23 Jinmai 23 20 C223 长治5608 Changzhi 5608 18
C13 晋麦24 Jinmai 24 18 C224 云麦766 Yunmai 766 21
C14 晋麦25 Jinmai 25 21 C225 临选2035 Linxuan 2035 22
C15 晋麦27 Jinmai 27 19 C226 临研151 Linyan 151 19
C16 晋麦28 Jinmai 28 20 C227 运麦2064 Yunmai 2064 22
C17 晋麦29 Jinmai 29 20 C228 圣麦20 Shengmai 20 22
C18 晋麦31 Jinmai 31 18 C229 圣麦104 Shengmai 104 22
C19 晋麦32 Jinmai 32 17 C230 晋麦107 Jinmai 107 18
C20 晋麦30 Jinmai 30 19 C231 品育8155 Pinyu 8155 22
C21 晋麦33 Jinmai 33 20 C232 冬黑1206 Donghei 1206 19
C22 晋麦35 Jinmai 35 17 C233 太紫6336 Taizi 6336 22
C23 晋麦36 Jinmai 36 21 C234 旱优6号 Hanyou 6 19
C24 晋麦37 Jinmai 37 19 C235 运85-24 Yun 85-24 21
C25 晋麦38 Jinmai 38 20 C236 晋麦10 Jinmai 10 20
C26 晋麦39 Jinmai 39 19 C237 晋麦89 Jinmai 89 21
C27 晋麦40 Jinmai 40 19 C238 晋麦6号 Jinmai 6 19
C28 晋麦41 Jinmai 41 18 C239 经麦6425 Jingmai 6425 18
C29 晋麦42 Jinmai 42 22 C240 紫优5号 Ziyou 5 19
C30 晋麦44 Jinmai 44 22 C241 紫麦8555 Zimai 8555 20
C31 晋麦43 Jinmai 43 18 C242 临麦5311 Linmai 5311 20
C32 晋麦45 Jinmai 45 24 C243 太615 Tai 615 19
C33 晋麦46 Jinmai 46 20 C244 长麦3809 Changmai 3809 19
C34 晋麦47 Jinmai 47 23 C245 长7170 Chang 7170 20
C35 晋麦48 Jinmai 48 19 C246 临农4357 Linnong 4357 18
C36 晋麦49 Jinmai 49 23 C247 晋麦109 Jinmai 109 20
C37 晋麦50 Jinmai 50 21 C248 早糯901 Zaonuo 901 21
C38 晋麦51 Jinmai 51 25 C249 临黑187 Linhei 187 18
C39 晋麦52 Jinmai 52 23 C250 临旱20 Linhan 20 19
C40 晋麦53 Jinmai 53 19 C251 尧麦30 Yaomai 30 23
C41 晋麦54 Jinmai 54 19 C252 尧麦31 Yaomai 31 21
C42 晋麦56 Jinmai 56 24 C253 昊麦66 Haomai 66 21
C43 晋麦57 Jinmai 57 23 C254 晋麦4号 Jinmai 4 18
C44 晋麦58 Jinmai 58 24 C255 晋春5号 Jinchun 5 20
C45 晋麦59 Jinmai 59 22 C256 梧麦168 Wumai 168 19
C46 晋麦60 Jinmai 60 25 C257 科紫6061 Kezi 6061 19
C47 晋麦61 Jinmai 61 21 C258 圣麦131 Shengmai 131 20
C48 晋麦62 Jinmai 62 26 C259 河东乌麦526 Hedongwumai 526 22
C49 晋麦63 Jinmai 63 22 C260 晋麦105 Jinmai 105 26
C50 晋麦65 Jinmai 65 21 C261 临糯88 Linnuo 88 18
C51 晋麦66 Jinmai 66 23 C262 晋麦108 Jinmai 108 21
C52 晋麦67 Jinmai 67 19 C263 晋麦34 Jinmai 34 22
C53 晋麦68 Jinmai 68 23 C264 晋太1503 Jintai 1503 19
C54 晋麦70 Jinmai 70 25 C265 济麦4075 Jimai 4075 18
C55 晋麦71 Jinmai 71 23 C266 翔支519 Xiangzhi 519 24
C56 晋麦72 Jinmai 72 21 C267 尧麦33 Yaomai 33 21
C57 晋麦73 Jinmai 73 20 C268 临旱19 Linhan 19 18
C58 晋麦74 Jinmai 74 21 C269 并麦1号 Bingmai 1 23
C59 晋麦75 Jinmai 75 24 C270 鲁原502 Luyuan 502 19
C60 临丰615 Linfeng 615 19

图1

小麦醇溶蛋白电泳图谱 1 : 大白麦; 2: 小红皮; 3: 牛趾甲(离石); 4: 红粒麦; 5: 绛州红; 6: 假红麦; 7: 牛趾甲(中阳); 8: 四月黄; 9: 三月黄; 10: 白芒糙; 11: 有芒大红茎;12: 竹秤青; 13: 红秃麦(屯留); 14: 白秃麦(屯留); 15: 白秃麦(吉县); 16: 红秃麦(吉县); 17: 叶蛐麦; 18: 红皮冬麦; 19: 白山疙瘩; 20: 红叶蛐; CK: 中国春。"

表1

山西小麦醇溶蛋白谱带数目的变化"

分类标准
Standard of classification
类型
Type
数量
Numbers
平均谱带数量
Average bands
分区Zone
α β γ ω
类型
Type
地方品种 Landrace 151 19.37±1.87 b 3.34±0.71 a 4.09±0.76 a 5.71±1.08 a 6.24±1.18 b
育成品种 Cultivar 270 20.96±2.21 a 3.34±0.91 a 4.29±0.98 a 5.61±1.12 a 7.72±1.60 a
旱地品种 Dryland cultivar 130 20.69±2.21 a 3.26±0.89 a 4.27±0.99 a 5.53±1.08 a 7.64±1.58 a
水地品种 Irrigated cultivar 140 21.21±2.20 a 3.42±0.91 a 4.31±0.97 a 5.68±1.16 a 7.79±1.61 a
麦区分类
Wheat region classification
北部春麦区
Northern spring wheat region
27 19.63±1.98 b 2.85±0.72 b 4.22±1.19 a 5.81±1.39 a 6.74±1.10 a
中部冬麦区
Middle winter wheat region
168 20.46±2.21 a 3.38±0.83 a 4.11±0.85 a 5.70±1.15 a 7.26±1.66 a
南部冬麦区
Southern winter wheat region
226 20.43±2.27 a 3.37±0.85 a 4.30±0.91 a 5.58±1.04 a 7.19±1.64 a

图2

不同品种类型(a, b)和生态分布(c)的山西小麦醇溶蛋白谱带变异范围统计"

图3

品种类型(a)和育成年代(b)对小麦醇溶蛋白遗传多样性的影响 PIC: 多态性信息含量。"

图4

山西小麦种质资源醇溶蛋白聚类图"

图5

同名小麦地方品种醇溶蛋白聚类图"

表2

同名地方品种醇溶蛋白谱带遗传分布"

名称
Name
生态区
Ecological zone
来源地
Origin
序号
Number
谱带数量
Number of bands
分区Zone
α β γ ω
和尚头
Heshangtou
中部麦区
Middle wheat-growing area
太谷Taigu L64 19 3 4 6 6
左权Zuoquan L72 20 3 4 6 7
崞县Guoxian L122 16 3 4 3 6
崞县Guoxian L123 21 3 4 7 7
南部麦区
Southern wheat-growing area
临汾Linfen L138 25 4 5 8 8
白秃麦
Baitumai
中部麦区
Middle wheat-growing area
石楼Shilou L92 21 4 4 5 8
南部麦区
Southern wheat-growing area
屯留Tunliu L14 17 3 4 3 7
吉县Jixian L15 18 3 4 5 6
红秃麦
Hongtumai
南部麦区
Southern wheat-growing area
屯留Tunliu L13 20 3 4 4 9
长治Changzhi L45 18 4 3 7 4
吉县Jixian L16 22 3 4 6 9

图6

地方品种“小红皮”表型和醇溶蛋白电泳图谱 图上为15个“小红皮”样本电泳图谱, 1~7为Hap I, 8~9为Hap II, 10~15为Hap III, 箭头所指为醇溶蛋白谱带出现变异的位置。"

表3

醇溶蛋白与面粉理化品质的相关分析"

相对迁移率
Rf
有无
谱带Bands
理化品质 Physical chemical quality
蛋白质含量
Protein content (%)
淀粉含量
Starch content (%)
纤维含量
Fiber content (%)
白度
Whiteness (%)
硬度指数
Hardness index
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
14.1 0 12.59 -0.160** 59.09 0.094 0.50 -0.059 69.83 -0.037 60.37 0.192**
1 12.30 59.51 0.47 69.56 62.73
16.5 0 12.64 -0.011 59.56 -0.008 1.18 -0.129* 71.95 -0.036 60.37 0.010
1 12.47 59.26 0.49 69.71 62.73
20.0 0 12.48 -0.068 59.22 0.122* 0.49 0.032 69.69 0.066 61.39 -0.100
1 12.01 61.29 0.56 71.55 56.91
21.3 0 12.56 -0.181** 59.06 0.172** 0.46 0.177** 69.63 0.046 61.29 0.006
1 12.18 59.95 0.59 70.03 61.38
23.4 0 12.38 0.121* 59.53 -0.140* 0.51 -0.085 69.93 -0.062 60.62 0.130*
1 12.59 58.92 0.46 69.48 62.20
24.4 0 12.52 -0.134* 59.12 0.141* 0.47 0.105 69.66 0.039 61.26 0.019
1 12.20 59.96 0.56 70.05 61.57
27.5 0 12.43 0.065 59.34 -0.054 0.52 -0.128* 69.70 0.010 60.99 0.071
1 12.55 59.10 0.43 69.77 61.89
34.0 0 12.30 0.167** 59.70 -0.177** 0.53 -0.109 69.91 -0.047 61.04 0.040
1 12.60 58.93 0.46 69.57 61.53
34.8 0 12.47 0.010 59.28 -0.034 0.48 0.099 69.89 -0.164** 61.04 0.049
1 12.50 59.00 0.59 67.67 61.53
35.3 0 12.50 -0.034 59.27 -0.007 0.48 0.035 70.18 -0.138* 61.36 -0.009
1 12.44 59.24 0.50 69.18 61.25
36.6 0 12.49 -0.032 59.31 -0.036 0.46 0.134* 70.13 -0.167** 61.38 -0.016
1 12.43 59.14 0.55 68.82 61.17
37.1 0 12.49 -0.034 59.29 -0.026 0.47 0.117* 70.13 -0.195** 61.12 0.055
1 12.42 59.16 0.55 68.51 61.89
37.9 0 12.43 0.111 59.38 -0.129* 0.48 0.051 69.97 -0.160** 61.25 0.022
1 12.70 58.62 0.53 68.40 61.59
38.7 0 12.47 0.116* 59.27 -0.116* 0.49 -0.138* 69.73 -0.019 61.30 0.039
1 14.23 54.92 0.25 68.52 65.13
39.8 0 12.54 -0.173** 59.09 0.177** 0.47 0.135* 69.52 0.130* 61.63 -0.127*
1 12.12 60.15 0.59 70.80 59.48
41.2 0 12.49 -0.056 59.18 0.077 0.48 0.088 69.48 0.151** 61.59 -0.098
1 12.36 59.63 0.55 70.94 60.04
45.2 0 12.45 0.157** 59.31 -0.156** 0.50 -0.174** 69.75 -0.043 61.37 -0.057
1 13.37 57.07 0.14 68.71 59.21
47.5 0 12.97 -0.088 57.39 0.133* 0.54 -0.025 71.25 -0.065 58.04 0.089
1 12.46 59.30 0.49 69.68 61.40
48.2 0 12.55 -0.155** 59.09 0.145* 0.49 -0.034 69.49 0.121* 61.33 -0.006
1 12.22 59.85 0.47 70.54 61.24
49.6 0 12.44 0.129* 59.32 -0.089 0.49 -0.020 69.68 0.037 61.51 -0.104
1 12.85 58.62 0.47 70.16 59.35
51.8 0 12.49 -0.099 59.22 0.085 0.48 0.128* 69.72 -0.005 61.31 0.073
1 12.03 60.21 0.69 69.64 61.30
52.4 0 12.33 0.124* 59.61 -0.128* 0.52 -0.070 69.92 -0.043 61.03 0.038
1 12.56 59.04 0.47 69.60 61.50
53.3 0 12.41 0.149** 59.36 -0.102 0.49 0.024 69.64 0.053 61.62 -0.110
1 12.77 58.77 0.51 70.14 59.89
54.4 0 12.48 -0.006 59.34 -0.019 0.42 0.119* 70.03 -0.046 61.16 0.014
1 12.47 59.24 0.51 69.63 61.36
55.4 0 12.41 0.145* 59.37 -0.095 0.50 -0.058 69.68 0.021 61.02 0.087
1 12.72 58.86 0.45 69.87 62.27
56.4 0 12.32 0.077 59.75 -0.099 0.60 -0.159** 69.70 0.003 61.09 0.017
1 12.50 59.16 0.47 69.73 61.36
59.9 0 12.46 0.138* 59.28 -0.096 0.49 -0.053 69.72 -0.011 61.32 -0.010
1 13.95 56.71 0.29 69.22 60.65
62.3 0 12.38 0.006 60.24 -0.026 0.78 -0.054 79.02 -0.149** 50.24 0.112
1 12.47 59.26 0.49 69.69 61.35
64.8 0 12.49 -0.062 59.20 0.076 0.47 0.124* 69.76 -0.028 61.28 0.015
1 12.33 59.71 0.59 69.45 61.55
68.0 0 12.50 −0.090 59.18 0.098 0.47 0.136* 69.64 0.058 61.58 -0.110
1 12.27 59.81 0.60 70.26 59.67
68.7 0 12.45 0.114* 59.29 -0.064 0.49 -0.041 69.73 -0.009 61.44 -0.088
1 12.92 58.64 0.43 69.57 59.06
70.0 0 12.50 -0.119* 59.18 0.140* 0.49 0.018 69.73 -0.012 61.28 0.020
1 12.07 60.43 0.51 69.55 61.76
74.1 0 12.33 0.235** 59.57 -0.211** 0.52 -0.124* 70.17 -0.181** 60.86 0.102
1 12.78 58.58 0.43 68.77 62.15
76.8 0 12.49 -0.021 59.37 -0.056 0.51 -0.065 70.14 -0.128* 60.59 0.140*
1 12.45 59.12 0.47 69.21 62.29

附表2

醇溶蛋白与面粉粉质和拉伸特性的相关分析"

相对
迁移率
Rf
有无谱带
Bands
面粉参数和面筋指标 Flour parameters and gluten index
吸水率
Water absorption (%)
形成时间
Development time (min)
稳定时间
Stability time (min)
粉质质量指数
Farinograph quality number (%)
弱化度/10
Degree of softing/10
弱化度/12
Degree of softing/12
均值
Mean
r
r
value
均值
Mean
r
r
value
均值
Mean
r
r
value
均值
Mean
r
r
value
均值
Mean
r
r
value
均值
Mean
r
r
value
14.1 0 59.34 0.028 1.49 0.233** 2.34 0.141* 31.76 0.213** 104.68 -0.167** 123.86 -0.090
1 59.56 1.88 2.87 38.75 93.65 117.73
16.5 0 58.80 0.009 1.58 0.005 3.50 -0.029 45.00 -0.037 72.00 0.050 94.00 0.049
1 59.43 1.65 2.55 34.56 100.29 121.55
20.6 0 59.50 -0.042 1.60 0.139* 2.45 0.142* 33.40 0.179** 102.54 -0.174** 123.51 -0.153**
1 59.02 1.93 3.19 41.59 86.55 108.95
21.3 0 59.67 -0.112 1.61 0.084 2.38 0.173** 33.28 0.150** 102.93 -0.155** 124.04 -0.147*
1 58.59 1.78 3.15 39.09 90.87 112.29
22.2 0 59.24 0.090 1.59 0.145* 2.48 0.072 33.46 0.133* 101.20 -0.058 121.76 -0.018
1 60.11 1.88 2.81 38.64 96.62 120.33
22.8 0 59.49 -0.053 1.67 -0.062 2.58 -0.046 35.04 -0.098 98.97 0.138* 120.44 0.107
1 58.67 1.47 2.25 28.95 116.52 133.77
23.4 0 58.88 0.150** 1.59 0.087 2.47 0.051 33.33 0.086 102.22 -0.068 123.40 -0.065
1 60.09 1.73 2.66 36.13 97.76 119.08
24.4 0 59.50 -0.042 1.61 0.109 2.52 0.041 34.25 0.049 100.47 -0.019 121.15 0.021
1 59.04 1.86 2.73 36.42 98.77 123.09
27.5 0 59.71 -0.097 1.63 0.037 2.38 0.128* 33.52 0.092 104.24 -0.174** 126.69 -0.218**
1 58.89 1.69 2.89 36.66 92.35 111.50
30.2 0 59.45 -0.016 1.65 -0.002 2.56 -0.003 34.69 -0.020 99.64 0.059 120.69 0.083
1 59.20 1.65 2.53 33.48 106.83 131.24
33.4 0 59.42 0.004 1.62 0.128* 2.53 0.049 34.25 0.065 100.63 -0.040 121.44 0.001
1 59.48 1.97 2.83 37.73 96.30 121.52
34.0 0 59.34 0.019 1.70 -0.054 2.59 -0.016 35.81 -0.065 99.83 0.010 123.14 -0.043
1 59.49 1.61 2.53 33.69 100.47 120.24
34.8 0 59.41 0.019 1.67 -0.059 2.56 -0.013 34.62 -0.005 99.34 0.091 120.32 0.116*
1 59.70 1.48 2.47 34.30 110.48 134.52
35.3 0 59.52 -0.024 1.64 0.015 2.37 0.110 32.72 0.126* 101.79 -0.053 122.01 -0.019
1 59.32 1.66 2.78 36.80 98.30 120.78
36.6 0 59.22 0.078 1.68 -0.048 2.63 -0.063 34.54 0.005 97.66 0.116* 118.13 0.146*
1 59.90 1.59 2.38 34.71 105.74 128.48
37.1 0 59.31 0.051 1.69 -0.073 2.65 -0.084 34.82 -0.024 97.82 0.126* 118.38 0.157**
1 59.79 1.55 2.29 33.92 107.20 130.27
37.9 0 59.36 0.040 1.67 -0.059 2.56 -0.006 34.37 0.031 100.02 0.013 121.08 0.025
1 59.80 1.54 2.53 35.75 101.13 123.36
39.8 0 59.52 -0.053 1.71 -0.163** 2.66 -0.129* 35.67 -0.153** 97.96 0.158** 119.35 0.142*
1 58.94 1.34 2.01 28.94 111.92 132.04
41.2 0 59.67 -0.132* 1.70 -0.121* 2.63 -0.091 35.61 -0.140* 99.00 0.082 120.80 0.043
1 58.23 1.43 2.18 29.52 106.17 124.65
43.3 0 59.14 0.107 1.51 0.257** 2.39 0.134* 32.75 0.169** 103.90 -0.169** 123.78 -0.108
1 60.07 1.96 2.93 38.65 92.07 115.92
43.8 0 59.68 -0.145* 1.59 0.179** 2.46 0.118* 33.70 0.130* 102.68 -0.180** 123.92 -0.179**
1 58.05 2.00 3.07 39.54 86.47 107.27
44.6 0 59.06 0.071 1.80 -0.138* 2.84 -0.118* 37.34 -0.132* 97.39 0.067 120.18 0.029
1 59.65 1.56 2.39 32.95 101.87 122.19
45.2 0 59.42 0.015 1.65 0.007 2.56 -0.025 34.65 -0.021 100.39 -0.039 121.79 -0.065
1 59.83 1.69 2.25 32.43 92.00 107.71
46.6 0 59.47 -0.023 1.60 0.146* 2.49 0.092 34.13 0.074 101.26 -0.085 122.14 -0.054
1 59.19 1.96 2.99 37.65 93.05 116.76
48.2 0 59.76 -0.153** 1.66 -0.010 2.38 0.181** 33.52 0.125* 102.06 -0.107 124.07 -0.149*
1 58.27 1.64 3.18 38.36 93.69 112.20
49.6 0 59.44 -0.005 1.68 -0.134* 2.64 -0.155** 35.49 -0.185** 99.21 0.100 120.91 0.053
1 59.36 1.29 1.60 24.68 111.00 127.16
50.3 0 59.47 -0.008 1.52 0.114* 2.18 0.142* 31.29 0.146* 104.67 -0.098 124.24 -0.061
1 59.41 1.72 2.74 36.26 97.92 120.00
51.1 0 59.50 -0.036 1.69 -0.085 2.65 -0.106 35.34 -0.096 98.87 0.085 120.57 0.054
1 59.12 1.51 2.14 31.34 105.95 125.13
52.8 0 59.43 -0.034 1.66 0.145* 2.59 0.123* 34.87 0.124* 99.88 -0.075 121.32 -0.017
1 59.49 1.38 1.75 27.45 108.55 124.82
53.3 0 59.64 -0.113* 1.71 -0.151** 2.57 -0.015 35.18 -0.081 99.86 0.023 122.03 -0.038
1 58.42 1.38 2.49 31.70 101.85 118.74
54.4 0 58.62 0.107 1.69 -0.026 2.90 -0.099 36.07 -0.049 93.38 0.113 113.73 0.125*
1 59.66 1.64 2.46 34.17 102.15 123.68
55.1 0 59.51 -0.099 1.66 -0.069 2.50 0.151** 34.46 0.040 100.68 -0.073 122.19 -0.107
1 57.48 1.37 3.94 37.75 88.58 104.33
55.4 0 59.33 0.048 1.63 0.062 2.57 -0.012 34.25 0.040 101.81 -0.094 123.23 -0.102
1 59.80 1.75 2.51 35.83 94.37 115.03
55.8 0 59.34 0.055 1.67 -0.066 2.65 -0.126* 35.30 -0.113* 98.17 0.162** 119.07 0.185**
1 60.01 1.51 1.95 29.85 113.77 137.26
57.3 0 59.42 0.010 1.66 -0.061 2.57 -0.049 34.80 -0.069 99.50 0.115* 120.82 0.100
1 59.64 1.38 2.06 28.60 120.30 139.00
64.1 0 58.43 0.120* 1.55 0.063 2.79 -0.062 34.48 0.003 102.8 -0.040 121.43 0.000
1 59.67 1.68 2.50 34.62 99.56 121.46
65.4 0 59.43 -0.002 1.61 0.246** 2.50 0.133* 34.03 0.152** 101.17 -0.131* 122.01 -0.081
1 59.39 2.53 3.63 45.33 81.73 108.58
65.8 0 59.43 -0.002 1.61 0.246** 2.50 0.133* 34.03 0.152** 101.17 -0.131* 122.01 -0.081
1 59.39 2.53 3.63 45.33 81.73 108.58
68.0 0 59.64 -0.138* 1.68 -0.092 2.51 0.058 34.44 0.024 100.09 0.008 121.63 -0.014
1 57.98 1.46 2.84 35.61 100.88 120.25
69.4 0 59.30 0.010 1.35 0.112 1.90 0.106 27.96 0.124* 116.32 -0.150** 137.04 -0.145*
1 59.44 1.68 2.61 35.20 98.73 119.98
70.0 0 59.46 -0.028 1.67 -0.067 2.57 -0.029 34.73 -0.033 99.09 0.130* 119.95 0.176**
1 58.99 1.44 2.35 32.53 116.47 144.17
74.1 0 59.19 0.085 1.68 -0.059 2.65 -0.072 35.60 -0.091 101.10 -0.041 123.52 -0.090
1 59.93 1.58 2.36 32.44 98.25 117.13
76.8 0 58.84 0.162** 1.52 0.182** 2.50 0.035 33.03 0.107 100.58 -0.013 120.31 0.039
1 60.15 1.82 2.63 36.52 99.72 122.91
79.8 0 59.09 0.128* 1.57 0.156** 2.52 0.028 33.60 0.094 100.68 -0.023 120.88 0.027
1 60.22 1.85 2.63 36.89 99.08 122.84
相对
迁移率
Rf
有无谱带
bands
面粉参数和面筋指标Flour parameters and gluten index
降落值
Falling number (s)
湿面筋含量
Wet gluten (%)
拉伸面积
Stretch area (cm2)
拉伸阻力
Extension resistance (E.U.)
延伸度
Extensibility (mm)
拉伸比例
Stretch ratio (%)
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
14.1 0 327.92 0.050 0.35 -0.093 37.03 0.190** 124.83 0.177** 184.13 -0.011 0.71 0.125*
1 334.76 0.34 46.84 148.75 183.34 0.81
16.5 0 312.50 0.016 0.34 0.019 44.50 -0.008 246.50 -0.099 118.50 0.110 2.10 -0.195**
1 330.76 0.35 41.08 134.3 184.03 0.75
20.6 0 333.18 -0.089 0.35 -0.070 39.37 0.164** 129.7 0.181** 184.07 -0.018 0.72 0.180**
1 316.27 0.34 51.17 163.81 182.26 0.93
21.3 0 330.97 -0.008 0.35 -0.145* 38.64 0.179** 127.32 0.206** 184.77 -0.051 0.71 0.202**
1 329.74 0.33 49.50 160.12 180.52 0.90
22.2 0 326.86 0.108 0.35 -0.106 39.63 0.109 130.60 0.118* 184.26 -0.024 0.73 0.112
1 344.45 0.34 46.29 149.49 182.19 0.84
22.8 0 330.32 0.021 0.35 -0.098 41.47 -0.053 135.43 -0.036 184.69 -0.089 0.75 -0.004
1 335.93 0.34 36.31 126.12 172.60 0.75
23.4 0 335.22 -0.075 0.34 0.067 38.98 0.091 130.84 0.065 179.74 0.127* 0.75 0.014
1 325.11 0.35 43.65 139.47 188.72 0.76
24.4 0 326.75 0.134* 0.35 -0.172** 40.52 0.053 132.23 0.089 184.20 -0.026 0.73 0.111
1 351.38 0.33 44.26 148.67 181.63 0.86
27.5 0 335.31 -0.097 0.34 0.096 37.72 0.183** 126.75 0.166** 180.37 0.134* 0.72 0.111
1 321.39 0.35 47.52 149.94 190.32 0.81
30.2 0 331.70 -0.052 0.35 0.057 40.95 0.020 135.08 -0.017 182.33 0.142* 0.76 -0.055
1 318.34 0.36 42.80 130.89 200.67 0.67
33.4 0 329.77 0.041 0.35 -0.027 40.74 0.043 133.05 0.078 184.28 -0.041 0.74 0.096
1 338.87 0.34 44.43 150.48 179.38 0.87
34.0 0 337.09 -0.084 0.41 0.152** 41.05 0.001 140.00 -0.067 179.06 0.115 0.80 -0.098
1 325.74 0.42 41.13 130.92 187.26 0.72
34.8 0 330.78 -0.004 0.35 -0.042 41.58 -0.066 135.67 -0.048 184.86 -0.104 0.75 -0.007
1 329.70 0.34 35.27 123.68 171.05 0.74
35.3 0 325.21 0.089 0.35 -0.016 41.42 -0.014 131.97 0.046 189.31 −0.172** 0.71 0.107
1 337.21 0.35 40.70 138.10 177.15 0.80
36.6 0 325.02 0.124* 0.35 -0.044 43.78 -0.155** 140.29 -0.122* 188.27 -0.186** 0.77 -0.061
1 342.90 0.34 35.32 122.82 174.19 0.71
37.1 0 328.46 0.056 0.35 -0.096 44.12 -0.208** 140.17 -0.142* 189.16 -0.265** 0.76 -0.044
1 337.08 0.34 31.90 118.24 167.51 0.72
37.9 0 329.94 0.025 0.35 0.048 42.86 -0.161** 139.03 -0.149* 185.32 −0.100 0.77 -0.105
1 334.56 0.35 31.62 111.72 175.62 0.65
39.8 0 328.69 0.067 0.35 -0.162** 42.41 -0.120* 137.54 -0.097 186.61 -0.185** 0.76 -0.052
1 340.93 0.35 34.04 119.76 168.7 0.70
41.2 0 332.80 -0.070 0.35 -0.020 42.26 -0.103 137.96 -0.109 182.85 0.061 0.77 -0.126*
1 320.26 0.35 35.16 118.35 188.69 0.63
43.3 0 327.85 0.063 0.35 0.047 37.16 0.226** 126.5 0.180** 180.22 0.148* 0.72 0.117*
1 337.01 0.35 49.43 152.16 191.40 0.82
43.8 0 327.43 0.112 0.35 -0.043 39.72 0.129* 132.16 0.092 182.81 0.067 0.74 0.044
1 348.24 0.34 48.91 149.41 189.43 0.79
44.6 0 338.00 -0.085 0.33 0.200** 43.58 -0.076 141.73 -0.081 179.68 0.090 0.80 -0.093
1 326.24 0.36 39.62 130.59 186.25 0.72
45.2 0 332.05 -0.129* 0.35 0.141* 40.97 0.032 134.69 0.006 183.37 0.078 0.75 -0.014
1 275.36 0.40 46.21 137.14 201.21 0.71
46.6 0 329.04 0.063 0.35 0.011 40.02 0.112 132.12 0.104 183.45 0.026 0.74 0.090
1 341.49 0.36 48.60 153.10 186.26 0.85
48.2 0 329.74 0.027 0.35 -0.166** 38.29 0.206** 126.52 0.231** 183.26 0.029 0.70 0.221**
1 334.06 0.33 50.87 163.40 185.70 0.92
49.6 0 333.06 -0.115* 0.35 0.100 41.74 -0.086 136.99 -0.114 182.89 0.088 0.77 -0.126*
1 305.40 0.37 33.72 109.02 194.30 0.58
50.3 0 333.04 -0.025 0.37 -0.141* 39.12 0.056 124.28 0.113 187.18 -0.069 0.67 0.140*
1 329.52 0.35 42.12 140.17 182.05 0.79
51.1 0 333.17 -0.077 0.35 0.065 42.53 -0.118* 138.91 -0.130* 183.66 0.008 0.78 -0.129*
1 319.77 0.36 34.89 116.77 184.42 0.64
52.8 0 330.65 0.099 0.35 -0.042 41.27 0.119* 135.38 0.120* 183.53 -0.001 0.76 0.110
1 331.86 0.35 36.15 117.30 191.45 0.61
53.3 0 331.77 -0.035 0.35 0.046 41.60 -0.043 135.69 -0.031 183.70 0.006 0.75 -0.018
1 325.63 0.35 38.72 130.26 184.27 0.73
54.4 0 334.92 −0.034 0.34 0.058 45.03 -0.080 142.80 -0.062 187.32 -0.051 0.78 -0.037
1 329.47 0.35 40.06 132.62 182.87 0.74
55.1 0 332.87 -0.163** 0.35 -0.129* 40.72 0.078 133.55 0.095 183.87 -0.010 0.74 0.104
1 275.00 0.31 51.55 167.90 182.00 0.98
55.4 0 327.82 0.082 0.34 0.142* 40.23 0.065 134.51 0.007 182.43 0.075 0.76 -0.033
1 341.19 0.37 44.29 135.63 188.90 0.72
55.8 0 329.23 0.056 0.35 -0.038 42.44 -0.138* 138.46 -0.145* 184.78 -0.072 0.77 -0.118*
1 340.27 0.34 32.00 109.64 177.23 0.62
57.3 0 330.55 0.012 0.35 -0.019 41.44 -0.072 135.45 -0.056 184.17 -0.055 0.75 -0.037
1 334.85 0.33 31.50 115.20 173.65 0.67
64.1 0 322.46 0.060 0.34 0.080 44.04 -0.056 151.33 -0.121* 176.27 0.104 0.89 -0.163**
1 332.69 0.35 40.40 130.82 185.59 0.72
65.4 0 329.54 0.074 0.35 -0.012 40.26 0.140* 132.06 0.172** 183.83 -0.003 0.73 0.176**
1 352.17 0.35 56.20 183.53 183.30 1.06
65.8 0 329.54 0.074 0.35 -0.012 40.26 0.140* 132.06 0.172** 183.83 -0.003 0.73 0.176**
1 352.17 0.35 56.20 183.53 183.30 1.06
68.0 0 333.10 -0.095 0.35 -0.056 41.76 -0.067 135.32 -0.022 185.31 -0.110 0.75 0.016
1 313.57 0.34 36.72 130.99 173.93 0.77
69.4 0 329.18 0.007 0.35 0.004 31.65 0.112 106.77 0.127* 173.50 0.088 0.60 0.111
1 330.84 0.35 41.96 137.30 184.74 0.76
70.0 0 329.53 0.066 0.39 -0.122* 41.46 -0.056 135.91 -0.067 184.78 -0.107 0.76 -0.053
1 347.58 0.35 35.61 117.39 169.25 0.67
74.1 0 333.25 -0.057 0.34 0.176** 41.72 -0.036 137.93 -0.069 180.56 0.133* 0.78 -0.089
1 325.02 0.36 39.78 128.12 190.56 0.70
76.8 0 318.59 0.201** 0.35 -0.008 38.60 0.109 128.78 0.099 183.35 0.014 0.72 0.086
1 345.88 0.35 44.15 142.05 184.35 0.79
79.8 0 323.70 0.156** 0.35 0.003 39.11 0.118* 129.74 0.113 183.54 0.011 0.73 0.089
1 346.48 0.35 45.59 146.07 184.40 0.81

表4

醇溶蛋白与面团黏度特性的相关分析"

相对
迁移率
Rf
有无谱带
Bnds
面团黏度(RVA) Dough viscosity (RVA)
峰值黏度
Peak value
(cp)
最低黏度
Low viscosity
(cp)
衰减值
Pad abort value
(cp)
最终黏度
Final viscosity
(cp)
回升值
Setback value
(cp)
峰值时间
Peak time
(min)
糊化温度
Gelatinization temperature (℃)
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
均值
Mean
r
r value
14.1 0 1811.28 0.114 972.08 0.131* 839.20 0.021 1942.71 0.142* 1942.71 0.151** 5.66 0.108 77.11 0.020
1 1950.17 1102.93 847.24 2198.10 2198.10 5.77 77.49
20.6 0 1875.71 -0.032 1034.60 -0.046 841.11 0.017 2068.78 -0.061 1034.18 -0.077 5.72 -0.091 77.72 -0.117*
1 1821.05 970.70 850.35 1916.51 945.81 5.59 74.63
22.2 0 1827.69 0.126* 997.91 0.106 829.78 0.127* 1993.00 0.114 995.09 0.122* 5.68 0.085 77.15 0.024
1 2010.81 1122.95 887.86 2237.63 1114.67 5.79 77.70
24.4 0 1829.23 0.147* 991.82 0.156** 837.41 0.061 1988.28 0.151** 996.46 0.140* 5.68 0.112 77.62 -0.087
1 2068.98 1200.09 868.89 2350.83 1150.74 5.84 75.42
31.4 0 1876.53 -0.112 1031.15 -0.092 845.39 -0.118* 2056.76 -0.089 1025.61 -0.083 5.71 -0.126* 77.24 0.021
1 1358.40 684.20 674.20 1452.00 767.80 5.19 78.77
33.4 0 1844.31 0.116* 1004.46 0.126* 839.85 0.041 2006.98 0.132* 1002.52 0.137* 5.68 0.106 77.55 -0.091
1 2072.67 1207.27 865.40 2392.30 1185.03 5.87 74.76
34.0 0 1976.30 -0.160** 1110.23 -0.153** 866.07 -0.110 2204.69 -0.158** 1094.45 -0.160** 5.78 -0.125* 76.88 0.037
1 1783.43 959.28 824.15 1923.67 964.39 5.64 77.57
42.7 0 1880.56 −0.093 1039.20 -0.123* 841.36 0.025 2072.92 -0.129* 1033.72 -0.133* 5.72 -0.112 77.16 0.047
1 1629.20 766.27 862.93 1555.60 789.33 5.44 79.15
43.3 0 1805.77 0.154** 981.57 0.133* 824.19 0.145* 1972.53 0.125* 990.96 0.112 5.68 0.068 77.74 -0.077
1 2005.14 1122.12 883.02 2210.48 1088.36 5.76 76.20
44.6 0 1964.45 -0.126* 1101.38 -0.121* 863.07 -0.085 2190.29 -0.127* 1088.91 -0.131* 5.77 -0.098 76.59 0.057
1 1808.68 978.78 829.90 1958.70 979.92 5.66 77.68
45.2 0 1881.89 -0.152** 1036.46 -0.147* 845.43 -0.101 2067.26 -0.151** 1030.80 -0.152** 5.72 -0.132* 77.37 -0.07
1 1287.43 566.29 721.14 1195.86 629.57 5.25 73.11
47.5 0 1417.29 0.118* 630.00 0.126* 787.29 0.046 1362.71 0.121* 732.71 0.112 5.24 0.135* 73.54 0.063
1 1878.71 1034.90 843.81 2063.17 1028.27 5.72 77.36
49.6 0 1886.32 -0.102 1039.25 -0.094 847.06 -0.080 2075.45 -0.108 1036.20 -0.121* 5.73 -0.130* 77.15 0.041
1 1668.00 874.84 793.1 1735.40 860.56 5.47 78.51
55.1 0 1883.72 -0.136* 1037.76 -0.130* 845.96 -0.094 2070.85 -0.140* 1033.09 -0.149* 5.71 -0.083 77.30 -0.016
1 1456.73 703.82 752.91 1420.55 716.73 5.48 76.49
56.4 0 1998.80 -0.099 1147.08 -0.113 851.72 -0.022 2281.80 -0.121* 1134.72 -0.127* 5.79 -0.072 76.97 0.015
1 1840.71 1000.15 840.56 1998.01 997.86 5.69 77.33
59.2 0 1790.85 0.061 966.74 0.057 824.11 0.046 1940.50 0.057 973.76 0.056 5.66 0.038 74.91 0.120*
1 1885.05 1038.44 846.61 2070.37 1031.93 5.71 77.80
59.9 0 1873.18 -0.111 1030.21 -0.123* 842.97 -0.032 2055.81 -0.128* 1025.60 -0.131* 5.71 -0.098 77.29 -0.035
1 1069.50 300.50 769.00 683.00 382.50 5.07 73.38
61.4 0 1866.75 0.015 1026.58 -0.027 840.17 0.120* 2050.11 -0.041 1023.53 -0.056 5.71 -0.112 77.36 -0.095
1 1958.67 894.00 1064.67 1691.33 797.33 5.11 68.55
65.4 0 1850.07 0.127* 1012.17 0.115* 837.90 0.104 2023.08 0.114 1010.90 0.110 5.69 0.080 77.42 -0.072
1 2194.27 1267.13 927.13 2479.40 1212.27 5.89 74.37
65.8 0 1850.07 0.127* 1012.17 0.115* 837.90 0.104 2023.08 0.114 1010.90 0.110 5.69 0.080 77.42 -0.072
1 2194.27 1267.13 927.13 2479.40 1212.27 5.89 74.37
67.3 0 1842.92 0.114 1010.10 0.085 832.83 0.141* 2017.90 0.089 1007.80 0.092 5.69 0.051 77.66 -0.118*
1 2056.35 1140.47 915.88 2263.82 1123.35 5.78 74.25
68.7 0 1883.67 -0.119* 1037.41 -0.111 846.27 -0.090 2071.17 -0.125* 1033.77 -0.139* 5.72 -0.172** 77.36 -0.047
1 1549.14 782.50 766.64 1553.50 771.00 5.29 75.30
73.2 0 1871.80 -0.052 1033.58 -0.129* 838.22 0.171** 2062.66 -0.139* 1029.08 -0.148* 5.72 -0.247** 77.29 -0.022
1 1631.00 544.20 1086.80 1112.20 568.00 4.69 75.74
76.8 0 1766.72 0.189** 935.25 0.206** 831.48 0.065 1879.53 0.212** 944.28 0.213** 5.62 0.175** 76.99 0.033
1 1994.28 1138.05 856.24 2255.72 1117.67 5.81 77.61
79.8 0 1807.03 0.152** 970.06 0.169** 836.97 0.044 1948.96 0.166** 978.90 0.157** 5.66 0.132* 77.22 0.008
1 2004.51 1149.66 854.86 2266.31 1116.66 5.81 77.38
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