大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析

doi:10.3724/SP.J.1006.2026.51068

摘要/Abstract

摘要：

解析亮氨酸富集重复型类受体激酶(leucine rich repeat receptor-like kinase，LRR-RLK)基因的功能和作用机制有利于揭示大麦发育和胁迫响应的分子机制。本研究以北青7号、Ynbs突变体和Morex等8个大麦品种(或品系)为材料，克隆了HvLRR-RLK-510基因的2904 bp CDS序列。8个材料HvLRR-RLK-510基因的CDS序列和编码蛋白氨基酸序列的相似性分别高于98.00%和99.70%。该蛋白具有亮氨酸富集重复结构域、跨膜结构域和激酶结构域等LRR-RLK的关键结构域，与小麦、水稻和拟南芥等植物LRR-RLK聚为1个亚类。RT-PCR检测和荧光定量检测结果表明，该基因在8个材料拔节期幼穗、叶鞘、叶、根和茎中差异表达，具有器官特异性和基因型依赖性，在幼穗中的表达更高效、基因型依赖性更低；亚细胞定位结果表明，编码蛋白位于本氏烟草叶细胞的细胞膜和液泡膜上。本研究为全面解析大麦HvLRR-RLK-510基因的功能和作用机制提供了基因资源和理论支持。

关键词: 大麦, 亮氨酸富集重复型类受体激酶, 基因克隆, 亚细胞定位, 表达特性

Abstract:

Elucidating the function and regulatory mechanisms of LRR-RLK (leucine-rich repeat receptor-like kinase) genes is essential for understanding the developmental processes and stress responses in barley. In this study, eight barley varieties or lines, including Beiqing 7, the Ynbs mutant, and Morex, were used as experimental materials. A 2904 bp coding sequence (CDS) of the HvLRR-RLK-510 gene was successfully cloned. The CDS and corresponding amino acid sequences of HvLRR-RLK-510 showed over 99.80% and 99.70% similarity, respectively, across the eight genotypes. The encoded protein contains key conserved domains typical of LRR-RLKs, including leucine-rich repeat domains, transmembrane regions, and a kinase domain. Phylogenetic analysis grouped this protein into a subclass shared with LRR-RLKs from wheat, rice, and Arabidopsis. RT-PCR and quantitative real-time PCR analyses revealed that HvLRR-RLK-510 was differentially expressed in young spikes, leaf sheaths, leaves, roots, and stems of the above-mentioned eight materials at the jointing stage, exhibiting both organ specificity and genotype dependence. Notably, expression in young spikes was consistently higher and less affected by genotype. Subcellular localization experiments showed that the protein is localized to the plasma membrane and vacuolar membrane in Nicotiana benthamiana leaf cells. This study provides a valuable gene resource and theoretical foundation for further investigation into the functional roles and molecular mechanisms of HvLRR-RLK-510 in barley.

Key words: barley, LRR-RLK, gene clone, subcellular localization, expression characteristics

余开航, 周洪斌, 罗亮扎, 王玫郦, 姜瑞梅, 董陈文华, 李仕金, 毛孝强, 陈升位. 大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析[J]. 作物学报, 2026, 52(2): 421-432.

Yu Kai-Hang, Zhou Hong-Bin, Luo Liang-Zha, Wang Mei-Li, Jiang Rui-Mei, Dong-Chen Wen-Hua, Li Shi-Jin, Mao Xiao-Qiang, Chen Sheng-Wei. Cloning and expression analysis of the HvLRR-RLK-510 gene encoding a leucine-rich repeat receptor-like kinase in barley[J]. Acta Agronomica Sinica, 2026, 52(2): 421-432.

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