马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析

doi:10.3724/SP.J.1006.2026.54094

摘要/Abstract

摘要： III类过氧化物酶(EC 1.11.1.7)是植物特异性氧化还原酶，广泛分布于生物体内，催化作为电子受体的过氧化氢(H₂O₂)与多种电子供体之间的氧化还原反应，是植物在胁迫条件下酶防御系统的关键酶之一。马铃薯(Solanum tuberosum L.)是茄科茄属的一年生草本植物，目前关于马铃薯POD基因家族(StPODs)的功能研究鲜有报道。本研究通过生物信息学的方法对StPODs基因家族成员进行了分析，探究其在多种非生物胁迫下的表达模式。马铃薯全基因组中共鉴定出148个StPODs基因家族成员，并根据基因在染色体上位置顺序依次命名为StPOD1~StPOD148。148个StPODs蛋白的长度为76~914个氨基酸不等，分子质量在83.64~101.32 kD之间；通过保守基序和结构域分析148个StPODs基因结构发现，所有StPODs基因均具有5个高度保守的Motif (Motif 1、Motif 2、Motif 3、Motif 4和Motif 5)和3个保守结构域(plant_peroxidase_like superfamily、secretory_peroxidase和PLN03030 superfanmily)。微阵列数据用于进一步的表达谱分析，多个StPODs基因受盐胁迫、干旱胁迫和高温胁迫后其表达量显著增加，其中响应盐胁迫的差异表达基因数量最多(64个)；经脱落酸(ABA)、生长素(IAA)、赤霉素(GA₃)和苄氨基嘌呤(BAP)处理后分别诱导了多个StPODs差异表达基因，其中85个StPODs基因受ABA诱导后差异表达。此外，本试验通过分析耐旱型马铃薯材料“A90”和干旱敏感型材料“A163”的表达谱，筛选出14个StPODs耐旱候选基因，它们在耐旱型材料和干旱敏感型材料之间的表达量表现出相反的趋势，将表达量趋势相差最大的6个候选基因在酒酿酵母中异源表达，证实了StPOD23和StPOD53基因参与酵母细胞的渗透调节反应。研究结果可为StPOD基因后续功能研究提供理论基础。

关键词: 过氧化物酶, 马铃薯, 非生物胁迫, 干旱胁迫, 表达谱分析

Abstract:

Class III peroxidases (EC 1.11.1.7) are plant-specific oxidoreductases widely distributed across plant species. They catalyze redox reactions between hydrogen peroxide (H₂O₂), serving as an electron acceptor, and various electron donors, playing a critical role in plant responses to diverse environmental stresses. Potato (Solanum tuberosum L.), a herbaceous annual of the Solanum genus in the Solanaceae family, has seen limited functional research on its peroxidase (POD) gene family (StPODs). In this study, we conducted a comprehensive bioinformatics analysis of the StPOD gene family to explore their expression patterns under various abiotic stresses. A total of 148 StPOD genes were identified in the potato genome and named StPOD1–StPOD148 based on their chromosomal positions. These StPOD proteins ranged from 76 to 914 amino acids in length, with an average length of 310 amino acids, and molecular weights ranging from 8.36 to 101.32 kDa. Functional analyses based on conserved motifs and structural domains revealed that all StPODs contained five highly conserved motifs (Motifs 1–5) and three conserved domains: plant_peroxidase_like superfamily, secretory_peroxidase, and PLN03030 superfamily. Microarray data were used to analyze their expression profiles under stress conditions. Many StPODs showed significantly increased expression in response to salt, drought, and high temperature stresses, with the highest number of differentially expressed genes (64) observed under salt stress. Additionally, several StPODs were induced by plant hormone treatments, including abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA₃), and benzylaminopurine (BAP), with 85 StPOD genes showing differential expression following ABA induction. Furthermore, by comparing expression profiles between the drought-tolerant potato line “A90” and the drought-sensitive line “A163”, 14 StPODs were identified as candidate drought-tolerance genes, showing opposite expression trends between the two lines. Six genes with the most pronounced differential expression were heterologously expressed in Saccharomyces cerevisiae, and functional analysis confirmed that StPOD23 and StPOD53 are involved in osmoregulatory responses in yeast cells. These findings provide a theoretical foundation for future functional studies of StPOD genes.

Key words: peroxidase, potato, abiotic stresses, drought stress, expression profile analysis

杨飚, 杜帅康, 张继旺, 石瑛, 张丽莉. 马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析[J]. 作物学报, 2026, 52(2): 405-420.

Yang Biao, Du Shuai-Kang, Zhang Ji-Wang, Shi Ying, Zhang Li-Li. Genome-wide identification of class III POD gene family in potato and its expression profile analysis[J]. Acta Agronomica Sinica, 2026, 52(2): 405-420.

参考文献

[1] Lee C J, Park S U, Kim S E, et al. Overexpression of IbLfp in sweetpotato enhances the low-temperature storage ability of tuberous roots. Plant Physiol Biochem, 2021, 167: 577–585.

[2] Passardi F, Cosio C, Penel C, et al. Peroxidases have more functions than a Swiss army knife. Plant Cell Rep, 2005, 24: 255–265.

[3] Kawano T. Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Rep, 2003, 21: 829–837.

[4] Foyer C H, Descourvières P, Kunert K J. Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Environ, 1994, 17: 507–523.

[5] Almagro L, Gómez Ros L V, Belchi-Navarro S, et al. Class III peroxidases in plant defence reactions. J Exp Bot, 2009, 60: 377–390.

[6] Welinder K G. Superfamily of plant, fungal and bacterial peroxidases. Curr Opin Struct Biol, 1992, 2: 388–393.

[7] Mathé C, Barre A, Jourda C, et al. Evolution and expression of class III peroxidases. Arch Biochem Biophys, 2010, 500: 58–65

[8] Hiraga S, Sasaki K, Ito H, et al. A large family of class III plant peroxidases. Plant Cell Physiol, 2001, 42: 462–468.

[9] Wu Y S, Yang Z L, How J, et al. Overexpression of a peroxidase gene (AtPrx64) of Arabidopsis thaliana in tobacco improves plant’s tolerance to aluminum stress. Plant Mol Biol, 2017, 95: 157–168.

[10] Wally O, Punja Z K. Enhanced disease resistance in transgenic carrot (Daucus carota L.) plants over-expressing a rice cationic peroxidase. Planta, 2010, 232: 1229–1239.

[11] Coego A, Ramirez V, Ellul P, et al. The H₂O₂-regulated Ep5C gene encodes a peroxidase required for bacterial speck susceptibility in tomato. Plant J, 2005, 42: 283–293.

[12] Choi H W, Kim Y J, Lee S C, et al. Hydrogen peroxide generation by the pepper extracellular peroxidase CaPO2 activates local and systemic cell death and defense response to bacterial pathogens. Plant Physiol, 2007, 145: 890–904.

[13] Valério L, De Meyer M, Penel C, et al. Expression analysis of the Arabidopsis peroxidase multigenic family. Phytochemistry, 2004, 65: 1331–1342.

[14] Passardi F, Longet D, Penel C, et al. The class III peroxidase multigenic family in rice and its evolution in land plants. Phytochemistry, 2004, 65: 1879–1893.

[15] Wang Y, Wang Q Q, Zhao Y, et al. Systematic analysis of maize class III peroxidase gene family reveals a conserved subfamily involved in abiotic stress response. Gene, 2015, 566: 95–108.

[16] Li G H, Manzoor M A, Wang G Y, et al. Comparative analysis of POD genes and their expression under multiple hormones in Pyrus bretschenedri. BMC Genom Data, 2024, 25: 41.

[17] Ren L L, Liu Y J, Liu H J, et al. Subcellular relocalization and positive selection play key roles in the retention of duplicate genes of Populus class III peroxidase family. Plant Cell, 2014, 26: 2404–2419.

[18] Behr M, Legay S, Hausman J F, et al. Analysis of cell wall-related genes in organs of Medicago sativa L. under different abiotic stresses. Int J Mol Sci, 2015, 16: 16104–16124.

[19] Cheng L T, Ma L X, Meng L J, et al. Genome-wide identification and analysis of the class III peroxidase gene family in tobacco (Nicotiana tabacum). Front Genet, 2022, 13: 916867.

[20] Romero A P, Alarcón A, Valbuena R I, et al. Physiological assessment of water stress in potato using spectral information. Front Plant Sci, 2017, 8: 1608.

[21] Wang X X, Shi M F, Zhang R Y, et al. Dynamics of physiological and biochemical effects of heat, drought and combined stress on potato seedlings. Chem Biol Technol Agric, 2024, 11: 109.

[22] Wang K T, Zhang H H, Yang L, et al. StMAPKK1 enhances drought and salt tolerance in potato via ROS scavenging and stomatal closure. Plant Physiol Biochem, 2025, 229: 110383.

[23] Letunic I, Khedkar S, Bork P. SMART: recent updates, new developments and status in 2020. Nucleic Acids Res, 2021, 49: 458-460.

[24] Wang J Y, Chitsaz F, Derbyshire M K, et al. The conserved domain database in 2023. Nucleic Acids Res, 2023, 51: 384-388.

[25] 季香林. 二倍体马铃薯种质资源抗旱性评价及抗旱基因的初步挖掘. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2022.
Ji X L. Drought Resistance Evaluation and Related Gene Perliminary Mining of Diploid Potato Germplasm Resources. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang, China, 2022 (in Chinese with English abstract).

[26] Tang X, Zhang N, Si H J, et al. Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress. Plant Methods, 2017, 13: 85.

[27] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^−ΔΔCT method. Methods, 2001, 25: 402–408.

[28] Hamilton A J, Bouzayen M, Grierson D. Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast. Proc Natl Acad Sci USA, 1991, 88: 7434–7437.

[29] Zhang C, Zhu P H, Zhang M Y, et al. Identification, classification and characterization of LBD transcription factor family genes in Pinus massoniana. Int J Mol Sci, 2022, 23: 13215.

[30] Xiao H L, Wang C P, Khan N, et al. Genome-wide identification of the class III POD gene family and their expression profiling in grapevine (Vitis vinifera L). BMC Genomics, 2020, 21: 444.

[31] Wu C L, Ding X P, Ding Z H, et al. The class III peroxidase (POD) gene family in cassava: identification, phylogeny, duplication, and expression. Int J Mol Sci, 2019, 20: 2730.

[32] Gao C Q, Wang Y C, Liu G F, et al. Cloning of ten peroxidase (POD) genes from Tamarix hispida and characterization of their responses to abiotic stress. Plant Mol Biol Report, 2010, 28: 77–89.

[33] Xiang X W, Song K K, Li Y Y, et al. Screening and expression analysis of POD gene in POD-H₂O₂ pathway on bud dormancy of pear (Pyrus pyrifolia). Forests, 2024, 15: 434.

[34] Zareen S, Ali A, Yun D J. Significance of ABA biosynthesis in plant adaptation to drought stress. J Plant Biol, 2024, 67: 175–184.

[35] Aleem M, Riaz A, Raza Q, et al. Genome-wide characterization and functional analysis of class III peroxidase gene family in soybean reveal regulatory roles of GsPOD40 in drought tolerance. Genomics, 2022, 114: 45–60.

[36] 陆雯佳, 汪军成, 姚立蓉, 等. 大麦PRX基因家族全基因组鉴定及其干旱胁迫下的表达分析. 作物学报, 2025, 51: 1198–1214.
Lu W J, Wang J C, Yao L R, et al. Genome-wide identification of PRX gene family and analysis of their expressions under drought stress in barley. Acta Agron Sin, 2025, 51: 1198–1214 (in Chinese with English abstract).

[37] Llorente F, López-Cobollo R M, Catalá R, et al. A novel cold-inducible gene from Arabidopsis, RCI3 encodes a peroxidase that constitutes a component for stress tolerance. Plant J, 2002, 32: 13–24.

[38] Kumar S, Jaggi M, Sinha A K. Ectopic overexpression of vacuolar and apoplastic Catharanthus roseus peroxidases confers differential tolerance to salt and dehydration stress in transgenic tobacco. Protoplasma, 2012, 249: 423–432.

[39] Zhu J Y, Chen L M, Li Z T, et al. Genome-wide identification of LOX gene family and its expression analysis under abiotic stress in potato (Solanum tuberosum L.). Int J Mol Sci, 2024, 25: 3487.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed