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作物学报 ›› 2026, Vol. 52 ›› Issue (2): 405-420.doi: 10.3724/SP.J.1006.2026.54094
杨飚(
), 杜帅康, 张继旺, 石瑛(), 张丽莉()
Yang Biao(), Du Shuai-Kang, Zhang Ji-Wang, Shi Ying(), Zhang Li-Li()
摘要：
III类过氧化物酶(EC 1.11.1.7)是植物特异性氧化还原酶, 广泛分布于生物体内, 催化作为电子受体的过氧化氢(H2O2)与多种电子供体之间的氧化还原反应, 是植物在胁迫条件下酶防御系统的关键酶之一。马铃薯(Solanum tuberosum L.)是茄科茄属的一年生草本植物, 目前关于马铃薯POD基因家族(StPODs)的功能研究鲜有报道。本研究通过生物信息学的方法对StPODs基因家族成员进行了分析, 探究其在多种非生物胁迫下的表达模式。马铃薯全基因组中共鉴定出148个StPODs基因家族成员, 并根据基因在染色体上的位置顺序依次命名为StPOD1~StPOD148。148个StPODs蛋白的长度为76~914个氨基酸不等, 分子质量在83.64~101.32 kD之间; 通过保守基序和结构域分析148个StPODs基因结构发现, 所有StPODs基因均具有5个高度保守的Motifs (Motif 1、Motif 2、Motif 3、Motif 4和Motif 5)和2个保守结构域(secretory_peroxidase和PLN03030 superfamily)。微阵列数据用于进一步的表达谱分析, 多个StPODs基因受盐胁迫、干旱胁迫和高温胁迫后表达量显著增加, 其中响应盐胁迫的差异表达基因数量最多(64个); 经脱落酸(ABA)、生长素(IAA)、赤霉素(GA3)和苄氨基嘌呤(BAP)处理后分别诱导了多个StPODs差异表达基因, 其中85个StPODs基因受ABA诱导后差异表达。此外, 本试验通过分析耐旱型马铃薯材料“A90”和干旱敏感型材料“A163”的表达谱, 筛选出14个StPODs耐旱候选基因, 它们在耐旱型材料和干旱敏感型材料之间的表达量表现出相反的趋势, 将表达量趋势相差最大的6个候选基因在酒酿酵母中异源表达, 证实了StPOD23和StPOD53基因参与酵母细胞的渗透调节反应。研究结果可为StPODs基因后续功能研究提供理论基础。
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