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作物学报

作物学报 ›› 2023, Vol. 49 ›› Issue (4): 1102-1110.doi: 10.3724/SP.J.1006.2023.24078

• 耕作栽培·生理生化 • 上一篇    下一篇

基于RGB颜色空间评价马铃薯块茎绿化程度

王硕(), 鲍天旸, 刘建刚, 段绍光, 简银巧, 李广存, 金黎平, 徐建飞*()   

  1. 中国农业科学院蔬菜花卉研究所/农业农村部薯类作物生物学和遗传育种重点实验室, 北京 100081
  • 收稿日期:2022-04-02 接受日期:2022-09-05 出版日期:2023-04-12 网络出版日期:2022-09-15
  • 通讯作者: *徐建飞, E-mail: xujianfei@caas.cn
  • 作者简介:E-mail: wangshuo202099@163.com
  • 基金资助:
    宁夏回族自治区农业育种专项(2019NYYZ01-1);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09)

Potato tuber greening evaluation based on RGB color space

WANG Shuo(), BAO Tian-Yang, LIU Jian-Gang, DUAN Shao-Guang, JIAN Yin-Qiao, LI Guang-Cun, JIN Li-Ping, XU Jian-Fei*()   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2022-04-02 Accepted:2022-09-05 Published:2023-04-12 Published online:2022-09-15
  • Contact: *E-mail: xujianfei@caas.cn
  • Supported by:
    Agricultural Breeding Project of Ningxia Hui Autonomous Region, China(2019NYYZ01-1);China Agriculture Research System of MOF and MARA(CARS-09)

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摘要：

马铃薯是世界上最重要的作物之一, 块茎作为马铃薯的商品器官, 块茎绿化严重影响马铃薯商品品质, 造成大量的资源浪费。建立一种方便快捷的块茎绿化评价方法, 对于马铃薯种质资源鉴定和块茎品质评价具有重要意义。本研究基于提取块茎颜色空间特征, 结合RGB颜色空间色差分析, 对15个马铃薯四倍体和二倍体品种(系)材料的绿化趋势和绿化程度进行了系统评价。供试材料块茎在25℃条件下, 125 μmol m-2 s-1光照强度下, 中薯2号、中薯18号、中薯19号、Atlantic、Favorita和Kondor的绿化速度较快, HS66的绿化速度较慢。在光照处理9 d后, 块茎色差值趋于稳定, 在光照处理13 d后, 不同品种绿化程度差异明显, 其中Favorita的绿化程度最大, 中薯28号的绿化程度最小。本研究建立的基于RGB颜色空间的马铃薯块茎绿化评价方法能够准确区分不同品种(系)的绿化速度和绿化程度, 该方法简便、高效, 结果可靠, 为精准评价块茎绿化程度并进行相关分析提供了技术方法参考。

关键词: 马铃薯, 块茎, 绿化, RGB颜色特征, 色差分析

Abstract:

Potato is one of the most important crops in the world. Tuber is the commercial product of potato, whereas tuber greening seriously affects product quality and results in a large amount of potatoes waste. It is very necessary to establish a convenient evaluation method of tuber greening for the identification of potato germplasm resources and the analysis of tuber quality. In this study, we systematically evaluated the greening trend and greening degree of 15 potato tetraploid and diploid varieties (lines) base on the extraction of tuber color space characteristics and RGB color space color difference. Zhongshu 2, Zhongshu 18, Zhongshu 19, Atlantic, Favorita, and Kondor appeared faster greening speed, but HS66 appeared slower greening speed under 25℃ and 125 μmol m-2 s-1. Color difference value of tubers of all potato varieties (lines) tended to the stability after 9 days lighting treatment. There was an obvious greening extent differences among potato varieties (lines). Among them, the afforestation degree of Favorita was the highest, whereas Zhongshu 28 was the lowest after 13 days lighting treatment. The method established in this study can accurately distinguish the greening speed and the extent of different varieties (lines), which provided a convenient and reliable technical reference for tuber greening evaluation.

Key words: potato, tuber, greening, RGB color features, color difference analysis

表1

2个块茎之间的相关性分析"

品种(系)
Variety (line)		 光照处理1 d后色差
Color difference after 1
day of light treatment		 光照处理7 d后色差
Color difference after 7
days of light treatment		 光照处理13 d后色差
Color difference after 13
days of light treatment
块茎1 Tuber 1 块茎2 Tuber 2 块茎1 Tuber 1 块茎2 Tuber 2 块茎1 Tuber 1 块茎2 Tuber 2
中薯2号 Zhongshu 2 59.82 59.09 48.74 47.01 46.75 44.41
中薯3号 Zhongshu 3 69.51 75.51 57.48 63.48 55.60 60.84
中薯5号 Zhongshu 5 71.11 73.81 56.70 61.73 53.81 57.32
中薯18号 Zhongshu 18 71.43 67.22 52.82 53.24 52.10 51.30
中薯19号 Zhongshu 19 78.00 75.58 61.13 60.40 59.16 59.40
中薯21号 Zhongshu 21 69.25 77.15 52.98 59.93 50.80 56.40
中薯27号 Zhongshu 27 71.13 72.22 46.01 52.16 44.51 50.35
中薯28号 Zhongshu 28 61.70 61.84 55.09 55.03 54.02 52.40
Atlantic 63.95 63.01 55.42 54.81 55.35 54.18
CE125 77.98 82.72 58.12 57.86 55.93 55.68
Favorita 71.19 68.72 43.29 42.83 41.35 40.73
HS66 54.10 65.57 47.75 56.09 46.28 53.19
Kondor 57.84 54.39 43.37 43.27 42.01 41.32
RH 76.38 73.28 56.73 51.73 54.91 49.30
Shepody 68.80 77.71 56.71 58.78 59.53 58.43
相关系数
Correlation coefficient		 0.785** 0.802** 0.823**

图1

不同品种(系)的色差变化趋势"

表2

供试品种(系)不同光照处理时间的块茎色差"

品种(系)
Variety (line)		 时间Time
0 d
0 DAL		 1 d后
1 DAL		 3 d后
3 DAL		 5 d后
5 DAL		 7 d后
7 DAL		 9 d后
9 DAL		 11 d后
10 DAL		 13 d后
13 DAL
中薯2号 Zhongshu 2 67.58 a 59.45 b 49.02 c 47.83 cd 47.88 cd 47.02 cd 45.98 d 45.58 d
中薯3号 Zhongshu 3 76.49 a 72.51 ab 64.56 bc 61.73 c 60.48 c 59.69 c 59.47 c 58.22 c
中薯5号 Zhongshu 5 73.48 a 72.46 a 65.90 b 60.26 bc 59.22 bc 56.57 c 56.25 c 55.56 c
中薯18号 Zhongshu 18 79.31 a 69.32 b 55.79 c 53.17 c 53.03 c 51.97 c 52.71 c 51.70 c
中薯19号 Zhongshu 19 85.00 a 76.79 b 64.97 c 61.59 d 60.76 de 60.35 de 60.18 de 59.28 e
中薯21号 Zhongshu 21 78.42 a 73.02 a 58.42 b 56.44 b 56.46 b 55.93 b 54.27 b 53.06 b
中薯27号 Zhongshu 27 74.82 a 71.68 a 57.45 b 49.80 bc 49.09 bc 47.16 c 46.88 c 47.43 c
中薯28号 Zhongshu 28 61.76 a 61.77 a 57.40 b 55.86 c 55.06 cd 53.94 de 53.97 de 53.21 e
Atlantic 71.77 a 63.48 b 56.46 c 54.54 c 55.11 c 54.53 c 54.36 c 54.76 c
CE125 84.68 a 80.35 a 65.76 b 58.82 c 57.99 c 56.99 c 56.00 c 55.81 c
Favorita 76.67 a 69.96 b 49.70 c 43.74 d 43.06 d 42.76 d 42.37 d 41.04 d
HS66 65.08 a 59.84 ab 53.52 ab 51.79 ab 51.92 ab 50.57 ab 48.67 b 49.73 ab
Kondor 59.32 a 56.11 b 47.59 c 44.26 d 43.32 d 42.21 d 42.06 d 41.67 d
RH 78.41 a 74.83 a 56.60 b 50.68 b 54.23 b 54.13 b 52.43 b 52.11 b
Shepody 78.36 a 73.25 a 62.70 b 59.53 b 57.74 b 60.00 b 58.72 b 58.98 b

图2

不同品种(系)间的绿化程度差异 采用Duncan’s检验, 图中误差线表示标准误, 不同小写字母表示在0.05概率水平差异显著。"

图3

供试品种(系)块茎绿化程度变化 DAL: 光照处理天数。"

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