-Ya;TANG Can-Ming;" /> Anatomic Analysis on Heterosis in Three Transgenic Bt Pest-Resistant Hybrid Cotton (<EM>G. hirsutum</EM> L.)
Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (03): 496-505.doi: 10.3724/SP.J.1006.2008.00496

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Anatomic Analysis on Heterosis in Three Transgenic Bt Pest-Resistant Hybrid Cotton (G. hirsutum L.)

ZENG Bin1,WANG Qing-Ya2,TANG Can-Ming1,*   

  1. 1 Agronomy College, Nanjing Agricultural University, 2 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
  • Received:2007-05-16 Revised:1900-01-01 Online:2008-03-12 Published:2008-03-12
  • Contact: TANG Can-Ming

Abstract: The anatomical characterization is the basis of physiological function of plants, so it is necessary to analyze heterosis performance and to reveal anatomical mechanism of heterosis. Anatomical characterization of the functional leaf in stem and vascular bundles of sympodial leaf stalk and boll stalk during flowering stage was investigated using three transgenic Bt pest-resistant cotton hybrids Nankang 3, Lumianyan 15, Xiangzamian 3, and their parents. The result showed that there were significant differences between hybrid and its parents in distribution of vascular bundles in the leaf nervures of the functional leaf (the fourth from top) in main stem. Three types of vascular bundles, three-offshoot, two-offshoot and no-offshoot were observed in leaf nervures of all tested materials. These were three-offshoot type in 3 hybrids and one of their parents, implying that the three-offshoot type was inherited dominantly. There were differences in the number of vessels, area of a vessel, area of all vascular bundles, area ratio of vascular to transection, leaf thickness, palisade tissue length, and palisade tissue width with different parents and hybrids. The total area of vascular bundles of functional leaf nervures in three hybrids were 0.72–0.77. The ratio of palisade tissue to spongy tissue of all hybrids was 1.12–1.15, suggesting that there existed transgressive or mid-parent heterosis in all hybrids. The total area of stomata per unit leaf area was a useful index for characteristic of stoma. There were transgressive heterosis in total area of stomata per unit leaf area on down leaf surface for three hybrids, but no heterosis on upper surface. There were negative correlation between two sides of leaf in the total area of stomata per unit leaf area (mm2 cm-2) of three hybrids, and significant negative correlation between stoma frequency and stama length (R2=0.5087), and positive correlation between stoma frequency and total area of stomata per unit leaf area (R2=0.5943) for upper surface of leaf but not significant for down surface of leaf. Moreover, there was significant positive correlation between leaf stalk of fruit branch and boll stalk in the number of vessel and area of a vessel, it demonstrated that the leaves on fruit branch have more contribution to the boll development than functional leaves in stem (R12=0.4791*, R22=0.4702*).

Key words: Transgenic Bt, G. hirsutum L., Vascular bundle, Heterosis, Stoma

[1] CHENG Yan-Shuang, HU Mei-Yan, DU Zhi-Min, YAN Bing-Chun, LI Li, WANG Yi-Wei, JU Xiao-Tang, SUN Li-Li, XU Hai. Effects of nitrogen reduction on stem vascular bundles, panicle and yield characters of RIL populations in Liaojing 5/Akitakaomaqi and their correlation [J]. Acta Agronomica Sinica, 2021, 47(5): 964-973.
[2] GUO Xue-Min,ZHAO Xiao-Man,XU Ke,WANG Xin-Rui,ZHANG Chen-Yu,DONG-FANG Yang. Anatomy and microscopic observation of Ricinus communis seed structure [J]. Acta Agronomica Sinica, 2020, 46(6): 914-923.
[3] XIANG Li-Yuan,XU Kai,SU Jing,WU Chao,YUAN Xiong,ZHENG Xing-Fei,DIAO Ying,HU Zhong-Li,LI Lan-Zhi. Genetic dissection of combining ability and heterosis of rice agronomic traits based on pathway analysis [J]. Acta Agronomica Sinica, 2019, 45(9): 1319-1326.
[4] Li-Li GUO,Xi-Xi ZHANG,Li-Hua HAO,Ya-Jun QIAO,Wen-Na CHEN,Yun-Ze LU,Fei LI,Xu CAO,Qing-Tao WANG,Yun-Pu ZHENG. Responses of leaf gas exchange to high temperature and drought combination as well as re-watering of winter wheat under doubling atmospheric CO2 concentration [J]. Acta Agronomica Sinica, 2019, 45(6): 949-956.
[5] Fei LI,Liang LIU,Hao ZHANG,Qing-Tao WANG,Li-Li GUO,Li-Hua HAO,Xi-Xi ZHANG,Xu CAO,Wei-Jia LIANG,Yun-Pu ZHENG. Effects of CO2 Concentrations on Stomatal Traits and Gas Exchange in Leaves of Soybean [J]. Acta Agronomica Sinica, 2018, 44(8): 1212-1220.
[6] Ying-Bin ZOU,Min HUANG. Opportunities and Challenges for Crop Production in China during the Transition Period [J]. Acta Agronomica Sinica, 2018, 44(6): 791-795.
[7] Qi-Yue WANG, Shu-Jun MENG, Ke ZHANG, Zhan-Hui ZHANG, Ji-Hua TANG, Dong DING. Investigation of Maize miRNA Involved in Developing-ear Heterosis [J]. Acta Agronomica Sinica, 2018, 44(6): 796-813.
[8] Jing DONG,Xiao-Ping LU,Kun-Ming ZHANG,Chun-Lei XUE,Rui-Xia ZHANG. Analysis of SNP and Allele-specific Expression in Transcriptome of Sorghum bicolor × Sorghum sudanense and Their Parents [J]. Acta Agronomica Sinica, 2018, 44(12): 1809-1817.
[9] Hai-Xia WU,Li-Li GUO,Li-Hua HAO,Hao ZHANG,Qing-Tao WANG,Dong-Juan CHENG,Zheng-Ping PENG,Fei LI,Xi-Xi ZHANG,Shu-Bin LI,Ming XU,Yun-Pu ZHENG. Effects of Water and CO2 Concentration on Stomatal Traits, Leaf Gas Exchange, and Biomass of Winter Wheat [J]. Acta Agronomica Sinica, 2018, 44(10): 1570-1576.
[10] ZHANG Jin-Fei, LI Xia, HE Ya-Fei,XIE Yin-Feng. Physiological Mechanism on Drought Tolerance Enhanced by Exogenous Glucose in C4-pepc Rice [J]. Acta Agron Sin, 2018, 44(01): 82-94.
[11] ZHANG Zheng,ZHANG Xue-Li,MO Bo-Cheng,Dai Zhi-Jun,HU Zhong-Li,LI Lan-Zhi,ZHENG Xing-Fei. Combining Ability Analysis ofAgronomic Trait in Indica × IndicaHybrid Rice [J]. Acta Agron Sin, 2017, 43(10): 1448-1457.
[12] YANG Hui-Li,LIN Ya-Nan,ZHANG Huai-Sheng,WEI Xiao-Yi,DING Dong,XUE Ya-Dong. Mapping of QTLs and Heterotic Loci for Flowering Time-related Traits in Maize [J]. Acta Agron Sin, 2017, 43(05): 678-690.
[13] WANG Sha,HE Yong,LUO Guang-Yu,YAO Min,ZHANG Xu,CHEN Xin-Bo,ZHOU Xiao-Yun. Influence of OsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resistance [J]. Acta Agron Sin, 2017, 43(03): 315-323.
[14] YU Ya-Hui,LIU Yu,LI Zhen-Yu,CHEN Guang-Hong,XU Zheng-Jin,TANG Liang,MAO Ting,XU Hai. Relationship between Indica-Japonica Index of Parents and Heterosis of Hybrid and Its Genetic Basis in Japonica Two Line Hybrid Rice [J]. Acta Agron Sin, 2016, 42(05): 648-657.
[15] HAN Ping-An,LU Xiao-Ping,MI Fu-Gui,ZHANG Rui-Xia,LI Mei-Na,XUE Chun-Lei,DONG Jing,CONG Meng-Lu. Analysis of Heterosis in Sorghum-Sudangrass Hybrids Seedlings Based on Proteomics [J]. Acta Agron Sin, 2016, 42(05): 696-705.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!