Abstract:

TEM and lead nitrate were used to locate Ca²⁺-ATPase in the anther development of Photoperiod-sensitive Genic Male-sterile Rice Nongken 58S and normal fertile rice Nongken 58N. The results showed that the quantity and the producing time of Ca²⁺-ATPase in the anther wall, connective tissue and pollen of fertile and sterile rice were very different. Some of Ca²⁺-ATPase distributed on the epidermal cells of fertile anther at pollen mother cell stage. And there were some Ca²⁺-ATPase in different anther wall cells, the more on the Ubisch body during the development of the pollen. Comparatively, the less and the later-produced Ca²⁺-ATPase distributed in the different anther wall cells of the sterile anther at the same stage. There were visible Ca²⁺-ATPase in the vacuole membrane of epidermal cell, cytoplasm of remained tapetum cell and the surface of Ubisch bodies at the binucleate pollen stage. Abundant Ca²⁺-ATPase distributed on the exine, the intine, the membrane of the cytoplasm and the nucleus of fertile pollen at the late uninucleate pollen stage, but no Ca²⁺-ATPase distributed on the sterile pollen during the development of the anther except binucleate pollen stage. The quantity of Ca²⁺-ATPase in the sterile pollen was less than that in the fertile pollen at the binucleate pollen stage. There were most Ca²⁺-ATPase in the fertile connective tissue at pollen mother cell meiosis stage. Comparatively, a little Ca²⁺-ATPase distributed in the sterile connective tissue until the pre-uninucleate pollen stage. Based on the above results, we suppose that the reason of pollen abortion is the less and later-produced Ca²⁺-ATPase distribution on the sterile pollen wall and the plasma membrane during the sterile anther development. And Ca²⁺ pump does not work for little Ca²⁺-ATPase in the sterile rice, so that it makes pollen abortion with abundant Ca²⁺ in the cytoplasm.

Key words: Photoperiod-sensitive genic male-sterility, Rice (Oryza sativa L.), Anther development, Ca²⁺-ATPase