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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1791-1797.doi: 10.3724/SP.J.1006.2009.01791


Molecular Mapping of Powdery Mildew Resistance Gene MlWE18 in Wheat Originated from Wild Emmer(Triticum turgidum var. dicoccoides)

HAN Jun1,2,ZHANG Lian-Song1,LI Gen-Qiao1,ZHANG Hong-Tao1,XIE Chao-Jie1,YANG Zuo-Min1,SUN Qi-Xin1,LIU Zhi-Yong1*   

  1. 1Department of Plant Genetics & Breeding,China Agricultural University/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Researdch & Utilization,Ministry of Education,Beijing 100193 China;2College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China
  • Received:2009-02-23 Revised:2009-04-30 Online:2009-10-12 Published:2009-08-07
  • Contact: LIU Zhi-Yong,E-mail: zhiyongliu@cau.edu.cn


Wild emmer (Triticum turgidum var. dicoccoides) is an important germplasm for wheat improvement especially for resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. Common wheat line 3D249 (Jingshuang 27//Yanda 1817/WE18/3/Wenmai 4, F7), a derivative of wild emmer accession WE18 and susceptible elite common wheat lines, was found highly resistant to prevailing powdery mildew isolate E09 at both seedling and adult plant stages in Beijing, China. Genetic analyses of the F1, F2 segregating population and their F3 progenies derived from a cross between susceptible line Xuezao and resistant line 3D249 indicated that the powdery mildew resistance of line 3D249 was controlled by a single dominant gene, temporarily designated MlWE18. By bulked segregant analysis (BSA), four SSR markers (Xwmc525, Xwmc273, Xcfa2040, and Xcfa2240), one RFLP-derived STS marker (Xmag1759) and one EST-STS marker (XE13-2) were found to be linked to MlWE18, with an order of Xwmc525–Xcfa2040–Xwmc273–XE13-2–Xmag1759–MlWE18–Xcfa2240 in the genetic linkage map. Using Chinese Spring nullisomic-tetrasomics, ditelosomics, and deletion lines, MlWE18 was physically mapped on chromosome 7AL terminal bin 7AL 16–0.85–1.00. However, the allelism of wild emmer derived MlWE18 to known powdery mildew resistance genes Pm1, PmU, MlIW72, Mlm2033, and Mlm80, all located on the same chromosome bin, need to be characterized in the future. The common wheat powdery mildew resistance line 3D249 provides useful new germplasm for disease resistance genes pyramiding and marker-assisted selection (MAS) in wheat breeding program.

Key words: Common wheat lin 3D249;Wild emmer(Triticum turgidum var.dicoccoides), Powdery mildew Resistance genes, Mlwe18, Molecular markers

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