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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1354-1360.doi: 10.3724/SP.J.1006.2012.01354

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Expression Patterns of TaPHYA Gene Subfamily in Common Wheat

WANG Xia1,2,**,MA Yan-Bin2,3,4,**,MENG Fan-Hua2,LI Xiu-Quan2,YANG Li2,Wu-Xia4,YANG Ke-Cheng3,*,YANG Jian-Ping2 ,*   

  1. 1 Department of Life Science, Yuncheng University, Yuncheng 044000, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Maize Research Institute, Sichuan Agricultural University, Ya’an 450002, China; 4 Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China
  • Received:2012-03-08 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-05
  • Contact: 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859; 杨克诚, Tel: 0835-2882465

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Abstract: Phytochromes (PHYs) are important genes relating to crop agronomic traits. In this paper, TaPHYA1, TaPHYA2, and TaPHYA3 were cloned fromcv. Chinese Spring(Triticum aestivum L.). Theputative domains ofTaPHYA1, TaPHYA2, and TaPHYA3 proteins were predicted via the NCBI Protein BLAST. Either TaPHYA1 or TaPHYA3 was composed of a GAF domain, a PHYtochrome domain, two PAS domains, a His Kinase A domain, and a Histidine kinase-like ATPase domain.Phylogenetic tree analysis indicated that TaPHYA1, TaPHYA2, and TaPHYA3 were closer to PHYA members of monocot plants (ZmPHYA, SbPHYA, and OsPHYA) rather than to those of dicot plants (AtPHYA and GmPHYA). Expression levels of TaPHYAs were analyzed using semi quantitative RT-PCR and real time PCR assays.Tissue-specific expression of total TaPHYA was also detected in stem, leaf, and spike,whose levelswere 1.35, 0.34, and 0.87 times of that in root. Additionally, TaPHYA showed high expression level in darkness, far-red, and blue light conditions, but low expression level in red and white light conditions. The transcription level of TaPHYA in the seedlings grown in the darkness or under far-red light was four or three times of that in seedlings grown in red light, respectively.

Key words: Triticum aestivum, Phytochrome A, Tissue-specific expression, Transcription expression analysis

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