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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1354-1360.doi: 10.3724/SP.J.1006.2012.01354

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Expression Patterns of TaPHYA Gene Subfamily in Common Wheat

WANG Xia1,2,**,MA Yan-Bin2,3,4,**,MENG Fan-Hua2,LI Xiu-Quan2,YANG Li2,Wu-Xia4,YANG Ke-Cheng3,*,YANG Jian-Ping2 ,*   

  1. 1 Department of Life Science, Yuncheng University, Yuncheng 044000, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Maize Research Institute, Sichuan Agricultural University, Ya’an 450002, China; 4 Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China
  • Received:2012-03-08 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-05
  • Contact: 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859; 杨克诚, Tel: 0835-2882465

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Abstract: Phytochromes (PHYs) are important genes relating to crop agronomic traits. In this paper, TaPHYA1, TaPHYA2, and TaPHYA3 were cloned fromcv. Chinese Spring(Triticum aestivum L.). Theputative domains ofTaPHYA1, TaPHYA2, and TaPHYA3 proteins were predicted via the NCBI Protein BLAST. Either TaPHYA1 or TaPHYA3 was composed of a GAF domain, a PHYtochrome domain, two PAS domains, a His Kinase A domain, and a Histidine kinase-like ATPase domain.Phylogenetic tree analysis indicated that TaPHYA1, TaPHYA2, and TaPHYA3 were closer to PHYA members of monocot plants (ZmPHYA, SbPHYA, and OsPHYA) rather than to those of dicot plants (AtPHYA and GmPHYA). Expression levels of TaPHYAs were analyzed using semi quantitative RT-PCR and real time PCR assays.Tissue-specific expression of total TaPHYA was also detected in stem, leaf, and spike,whose levelswere 1.35, 0.34, and 0.87 times of that in root. Additionally, TaPHYA showed high expression level in darkness, far-red, and blue light conditions, but low expression level in red and white light conditions. The transcription level of TaPHYA in the seedlings grown in the darkness or under far-red light was four or three times of that in seedlings grown in red light, respectively.

Key words: Triticum aestivum, Phytochrome A, Tissue-specific expression, Transcription expression analysis

[1]Wang H, Deng X W. Dissecting the phytochrome A-dependent signaling network in higher plants. Trends Plant Sci, 2003, 8: 172–178

[2]Schepens I, Duek P, Fankhauser C. Phytochrome-mediated light signalling in Arabidopsis. Curr Opin Plant Biol, 2004, 7: 564–569

[3]Garg A K, Sawers R J H, Wang H, Kim J K, Walker J M, Brutnell T P, Parthasarathy M V, Vierstra R D, Wu R J. Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield. Planta, 2006, 223: 627–636

[4]Thiele A, Herold M, Lenk I, Quail P H, Gatz C. Heterologous expression of Arabidopsis phytochrome B in transgenic potato influences photosynthetic performance and tuber development. Plant Physiol, 1999, 120: 73–81

[5]Dehesh K, Tepperman J, Christensen A H, Quail P H. phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet, 1991, 225: 305–313

[6]Basu D, Dehesh K, Schneider-Poetsch H J, Harrington S E, McCouch S R, Quail P H. Rice PhyC gene: structure, expression, map position and evolution. Plant Mol Biol, 2000, 44: 27–42

[7]Takano M, Kanegae H, Shinomura T, Miyao A, Hirochika H, Furuya M. Isolation and characterization of rice phytochrome A mutants. Plant Cell, 2001, 13: 521–534

[8]Takano M, Inagaki N, Xie X, Yuzurihara N, Hihara F, Ishizuka T, Yano M, Nishimura M, Miyao A, Hirochika H, Shinomura T. Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice. Plant Cell, 2005, 17: 3311–3325

[9]Sheehan M J, Farmer P R, Brutnell T P. Structure and expression of maize phytochrome family homeologs. Genetics, 2004, 167: 1395–1405

[10]Gaut B S, Doebley J F. DNA sequence evidence for the segmental allotetraploid origin of maize. Proc Natl Acad Sci USA, 1997, 94: 6809–6814

[11]Ma Y-B(马燕斌), Li Z(李壮), Cai Y-F(蔡应繁), Zhou P(周朋), Xiao Y(肖阳), Huang Y-B(黄玉碧), Fu F-L(付凤玲), Pan G-T(潘光堂), Yang K-C(杨克诚), Yang J-P (杨建平). Isolation, protein structures and expression patterns responding to different light treatments of two phytochrome A genes in maize (Zea mays L.). Sci Agric Sin (中国农业科学), 2010, 43(10): 1985–1993 (in Chinese with English abstract)

[12]Wu F Q, Zhang X M, Li D M, Fu Y F. Ectopic expression reveals a conserved PHYB homolog in soybean. PLoS One, 2011, 6: e27737. DOI: 10.1371/journal.pone.0027737

[13]Ogihara Y, Shimizu, H, Hasegawa K, Tsujimoto H, Sasakuma T. Chromosome assignment of four photosynthesis-related genes and their variability in wheat species. Theor Appl Genet, 1994, 88: 383–394

[14]Kulshreshtha R, Kumar N, Balyan H S, Gupta P K, Khurana P, Tyagi A K, Khurana J P. Structural characterization, expression analysis and evolution of the red/far-red sensing photoreceptor gene, phytochrome C (PhyC), localized on the ‘B’ genome of hexaploid wheat (Triticum aestivum L.). Planta, 2005, 221: 675–689

[15]Li Z(李壮), Ma Y-B (马燕斌), Cai Y-F(蔡应繁), Wu S-W(吴锁伟), Xiao Y(肖阳), Meng F-H(孟凡华), Fu F-L(付风铃), Huang Y-B(黄玉碧), Yang J-P(杨建平). Cloning and expression analysis of TaPhyB3 in Triticum aestivum. Acta Agron Sin (作物学报), 2010, 36(5): 779–787 (in Chinese with English abstract)

[16]Devos K M, Beales J, Ogihara Y, Doust A N. Comparative sequence analysis of the Phytochrome C gene and its upstream region in allohexaploid wheat reveals new data on the evolution of its three constituent genomes. Plant Mol Biol, 2005, 58: 625–641

[17]Yu B-L(余波澜), Huang C-F(黄朝峰), Zhou W-J(周文娟), Zhang W-J(张文俊). Evolution study of wheat (Tritium aestivum L.) A, B and D genome based on DNA sequence similarity. Acta Genet Sin (遗传学报), 2001, 28(7): 635–639 (in Chinese with English abstract)

[18]Yu H-X (于海霞), Tian J-C(田纪春). Review of genome B in T. aestivum L. Mol Plant Breed (分子植物育种), 2008, 6(4): 724–732 (in Chinese with English abstract)

[19]Clough R C, Jordan-Beebe E T. Sequences within both the N- and C-terminal domains of phytochrome A are required for PFR ubiquitination and degradation. Plant J, 1999, 17: 155–167

[20]Weller J L, Batge S L, Smith J J, Kerckhoffs L H, Sineshchekov V A, Murfet I C, Reid J B. A dominant mutation in the pea PHYA gene confers enhanced responses to light and impairs the light-dependent degradation of phytochrome A. Plant Physiol, 2004, 135: 2186–2195

[21]Müller R, Fernández A P, Hiltbrunner A, Schäfer E, Kretsch T. The histidine kinase-related domain of Arabidopsis phytochrome A controls the spectral sensitivity and the subcellular distribution of the photoreceptor. Plant Physiol, 2009, 150: 1297–1309

[22]Hennig L, Buche C. Dynamic properties of endogenous phytochrome A in Arabidopsis seedlings. Plant Physiol, 1999, 121: 571–577

[23]Quail P H. Phytochrome: A light-activated molecular switch that regulates plant gene expression. Annu Rev Genet, 1991, 25: 389–409

[24]Wang H, Ma L, Habashi J, Li J, Zhao H, Deng X W. Analysis of far-red light-regulated genome expression profiles of phytochrome A pathway mutants in Arabidopsis. Plant J, 2002, 32: 723–733

[25]Canton F R, Quail P H. Both phyA and phyB mediate light-imposed repression of PhyA gene expression in Arabidopsis. Plant Physiol, 1999, 121: 1207–1215

[26]Somers D E, Quail P H. Temporal and spatial expression patterns of PhyA and PhyB genes in Arabidopsis. Plant J, 1995, 7: 413–427
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