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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1424-1434.doi: 10.3724/SP.J.1006.2014.01424

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Subdivision of Nitrogen Use Efficiency of Rice Based on 15N Tracer

LIN Jing-Jing1,LI Gang-Hua1,XUE Li-Hong2,*,ZHANG Wu-Jun1,XU Hui-Ge1,WANG Shao-Hua1,YANG Lin-Zhang2,DING Yan-Feng1,*   

  1. 1 Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China?
  • Received:2014-01-17 Revised:2014-04-16 Online:2014-08-12 Published:2014-06-03
  • Contact: 薛利红, E-mail: njxuelihong@gmail.com; 丁艳锋, E-mail: dingyf@njau.edu.cn, ?025-84395033

Abstract:

The nitrogen (N) uptake, and N use efficiencies (NUE) at different rice growth stages (i.e. during basal, tillering and panicle fertilizations) were studied using 15N isotope tracing. A two-year field experiment with two N rates and two distribution ratios was conducted using two different high-yielding rice cultivars Wuyunjing 23 (japonica) and Yliangyou 2 (hybrid indica). A sub-plot of 15N isotope tracing experiment with three duplications under the same treatment was also set up in the field. The results revealed that basal nitrogen absorbed by rice was only 1.5%–11.5% before tillering fertilization (eight days after transplanting), 6.6%–24.9% from tillering fertilization to panicle fertilization, and little after panicle fertilization. The overall recovery efficiency of basal N (NUEB) was low and ranged from 9.1% to 22.8%, not significantly affected by different cultivars and N treatments. Tillering fertilizer N was mainly absorbed from tillering fertilization to panicle fertilization, and no longer had effect after panicle fertilization. NUE of tillering N fertilizer (NUET) was 17%–34%, which is almost the same as that of basal fertilizer. NUET of Yliangyou 2 was higher than that of Wuyunjing 23. Compared with basal and tillering N fertilizers, NUE of panicle N fertilizer (NUEP) was the highest with a value of 54.0%–82.1%, and Wuyunjing 23 had lower NUE than Yliangyou 2. The whole NUE in the entire growth period of all N fertilizers decreased with the increase of N application rate, and varied from 32% to 64%. Among the total N uptake of rice, the contribution was 4.13%–10.59% (average 6.92%) for basal N fertilizer, 3.98%–11.75% (average 7.58%) for tillering fertilizer, 13.32%–37.56% (average 26.02%) for panicle fertilizer, and 45.71%–70.83% (average 59.91%) for the soil. The experiment also revealed that the more the basal and tillering fertilizers applied, the lower the total NUE. It is suggested that rice N management, the N absorption and utilization from fertilizer applied at different stages should be considered to improve the NUE of rice, and ensure the hig yield while avoid the N loss.

Key words: Rice, Nitrogen management;, 15N, nitrogen application at different stages, Nitrogen use efficiency

[1]彭少兵, 黄见良, 钟旭华, 杨建昌, 王光火, 邹应斌, 张福锁, 朱庆森, Buresh R, Witt C. 提高中国稻田氮肥利用率的研究策略. 中国农业科学, 2002, 35: 1095–1103



Peng S B, Huang J L, Zhong X H, Yang J C, Wang G H, Zhou Y B, Zhang F S, Zhu Q S, Buresh R, Witt C. Research strategy in improving fertilizer nitrogen use efficiency of irrigated rice in China. Sci Agric Sin, 2002, 35: 1095–1103 (in Chinese with English abstract)



[2]李庆逵. 中国农业持续发展中的肥料问题. 南昌: 江西科学技术出版社, 1997. pp 38–48



Li Q K. Fertilizer Issues in the Sustainable Development of China Agriculture. Nanchang: Jiangxi Science and Technology Press, 1997. pp 38–48 (in Chinese)



[3]Zhang J, Zhang H C, Duan X M, Xu Z J, Yang B, Guo B W, Du B, Dai Q G, Xu K, Huo Z Y, Wei H Y. Effects of soil fertility and nitrogen application rates on super rice yield, quality, and nitrogen use efficiency. Acta Agron Sin, 2012, 37(11): 2020–2029



[4]刘立军, 徐伟, 桑大志, 刘翠莲, 周家麟, 杨建昌. 实地氮肥管理提高水稻氮肥利用效率. 作物学报, 2006, 32: 987–994



Liu L J, Xu W, Sa D Z, Liu C L, Zhou J L, Yang J C. Site-specific nitrogen management increases fertilize-nitrogen use efficiency in rice. Acta Agron Sin, 2006, 32: 987–994 ( in Chinese with English abstract)



[5]万靓军, 张洪程, 霍中洋, 林忠成, 戴其根, 许轲, 张军. 氮肥运筹对超级杂交粳稻产量、品质及氮素利用率的影响. 作物学报, 2007, 33: 175–182



Wang L J, Zhang H C, Huo Z Y, Lin Z C, Dai Q G, Xu K, Zhang J. Effects of soil fertility and nitrogen application rates on super rice yield, quality, and nitrogen use efficiency. Acta Agron Sin, 2007, 33: 175–182 (in Chinese with English abstract)



[6]Ladha J K, Pathak H, Krupnik T J, Six J, van Kessel C. Efficiency of fertilizer nitrogen in cereal production: retrospects and prospects. Adv Agron, 2005, 87: 85–156 



[7]王绍华, 曹卫星, 丁艳锋, 刘胜环, 王强盛. 基本苗数和施氮量对水稻氮吸收与利用的影响. 南京农业大学学报, 2003, 26(4): 1–4



Wang S H, Cao W X, Ding Y F, Liu S H, Wang Q S. Effects of planting density and nitrogen application rate on absorption and utilization of nitrogen in rice. J Nanjing Agric Univ, 2003, 26(4): 1–4 (in Chinese with English abstract)



[8]黄见良, 水稻氮素营养特性、氮肥利用率与实时实地氮肥管理的研究. 湖南农业大学博士学位论文, 2003



Huang J L. Studies on Nitrogen Uptake Characteristics, Nitrogen Use Effieieney, Real Time Nitrogen Management, and Site Specific Nutrient Management Of Irrigated Rice. PhD Dissertation of Hunan Agricultural University, 2003 (in Chinese with English abstract)



[9]Cassman K G, Peng S B, Olk D C, Ladha J K, Reichardt W, Dobermann A, Singh U. Opportunities for increased nitrogen-use efficiency from improved resource management in irrigated rice systems. Field Crops Res, 1998, 56: 7–39



[10]黄见良, 邹应斌, 彭少兵, Buresh R J. 水稻对氮素的吸收、分配及其在组织中的挥发损失. 植物营养与肥料学报, 2004, 10: 579–583



Huang J L, Zou Y B, Peng S B, Buresh R J. Nitrogen uptake, distribution by rice and its losses from plant tissues. Plant Nutr Fert Sci, 2004, 10 : 579–583 (in Chinese with English abstract)



[11]丁艳锋, 赵长华, 王强盛. 穗肥施用时期对水稻氮素利用及产量的影响. 南京农业大学学报, 2003, 26(4): 5–8



Ding Y F, Zhao C H, Wang Q S. Effect of application stage of panicle fertilizer on rice grain yield and utilization of nitrogen. J Nanjing Agric Univ, 2003, 26(4): 5–8 (in Chinese with English abstract)



[12]凌启鸿, 张洪程, 戴其根, 丁艳锋, 凌励, 苏祖芳, 徐茂, 阙金华, 王绍华. 水稻精确定量施氮研究. 中国农业科学, 2005, 38: 2457−2467



Ling Q H, Zhang H C, Dai Q G, Ding Y F, Ling L, Su Z F, Xu M, Que J H, Wang S H. Study on precise and quantitative N application in rice. Sci Agric Sin, 2005, 38: 2457−2467 (in Chinese with English abstract)



[13]Huang J L, He F, Cui K, Buresh RJ, Xu B, Gong W, Peng S B. Determination of optimal nitrogen rate for rice varieties using a chlorophyll meter. Field Crops Res, 2008, 105: 70–80



[14]王维金. 关于不同籼稻品种和施肥时期稻株对15N的吸收及其分配的研究. 作物学报, 1994, 20: 476–480



Wang W J. Studies on rice uptake and distribution of 15N fertilizer in different varieties of indica rice and at different growth stages. Acta Agron Sin, 1994, 20: 476–480 ( in Chinese with English abstract)



[15]赵广才, 张保明, 王崇义. 应用15N研究小麦各部位氮素分配利用及施肥效应. 作物学报, 1998, 24: 854–480



Zhao G C, Zhang B Y, Wang C Y. Studies on the effect of fertilization and distribution utilization of nitrogen in different parts of wheat by using 15N tracer technique. Acta Agron Sin, 1998, 24: 854–858 ( in Chinese with English abstract)



[16]赵广才, 何中虎, 田奇卓, 李克民, 刘利华, 李振华, 张文彪. 应用15N研究施氮比例对小麦氮素利用的效应. 作物学报, 2004, 30: 159–162



Zhao G C, He Z H, Tian Q Z, Li K M, Liu L H, Li Z H, Zhang W B. Effect of application rate of nitrogen on its utilization in wheat by using 15N tracer technique. Acta Agron Sin, 2004 , 30: 159–162 ( in Chinese with English abstract)



[17]鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000. pp 558–564



Lu R K. Agricultural Chemical Analysis Method of Soil. Beijing: China Agricultural Science and Technology Press, 2000. pp 558–564 (in Chinese)



[18]石玉, 于振文, 李延奇, 王雪. 施氮量和底追肥比例对冬小麦产量及肥料氮去向的影响. 中国农业科学, 2007, 40: 54–62



Shi Y, Yu Z W, Li Y Q, Wang X. Effects of nitrogen fertilizer rate and ratio of base and topdressing on winter wheat yield and fate of fertilizer nitrogen. Sci Agric Sin, 2007, 40: 54–62 (in Chinese with English abstract)



[19]晏娟, 沈其荣, 尹斌, 万新军. 应用15N示踪技术研究水稻对氮肥的吸收和分配. 核农学报, 2009, 23: 487–491



Yan J, Sheng Q R, Yan B, Wang X J. Fertilizer-N uptake and distribution in rice plants using 15N tracer technique. Acta Agric Nucl Sin, 2009, 23: 487–491 (in Chinese with English abstract)



[20]Takahashi S, Yagi A. Losses of fertilizer-derived N from transplanted rice after heading. Plant Soil, 2002, 242: 245–250



[21]徐阳春, 吴小庆, 郭世伟, 沈其荣. 水稻生育后期地上部氨挥发与氮素利用效率的研究. 植物营养与肥料学报, 2008, 14(2): 207–212



Xu Y C, Wu X Q, Guo S W, Shen Q R. Nitrogen use efficiency and ammonia volatilization from rice shoot in late growth stages. Plant Nutr Fert Sci, 2008, 14(2): 207–212 (in Chinese with English abstract)



[22]汪华, 杨京平, 金洁, 孙军华. 不同氮素用量对高肥力稻田水稻-土壤-水体氮素变化及环境影响分析. 水土保持学报, 2006, 20(1): 50–54



Wang H, Yang J P, Jin J, Sun J H. N variation in rice-soil-water system under different N application level in high-yielding paddy field and its environment effect. J Soil Water Conserv, 2006, 20(1): 50–54 (in Chinese with English abstract)



[23]Wang X, Suo Y, Feng Y, Shohag M J I, Gao J, Zhang Q, Xie S, Lin X. Recovery of (15)N–labeled urea and soil nitrogen dynamics as affected by irrigation management and nitrogen application rate in a double rice cropping system. Plant Soil, 2011, 343: 195–208



[24]符建荣. 控释氮肥对水稻的增产效应及提高肥料利用率的研究. 植物营养与肥料学报, 2001, 7: 145–152



Fu R. Effects of controlled release ferlizer on rice yield and N recovery. Plant Nutr Fert Sci, 2001, 7: 145–152 (in Chinese with English abstract)



[25]Pan S G, Huang S Q, Zhai J, Wang J P, Cao C G, Cai M L, Zhan M, Tang X R. Effects of N management on yield and N uptake of rice in central China. Sci Agric Sin, 2012, 11: 1993–2000



[26]Wada G, Aragones R C, Ando H. Effect of slow release fertilizer(meister) on the nitrogen uptake and yield of the rice plant in the tropics. Jpn J Crop Sci, 1991, 60: 101–106



[27]周伟, 田玉华, 尹斌. 太湖地区水稻追肥的氨挥发损失和氮素平衡. 中国生态农业学报, 2011, 19(1): 32–36



Zhou W, Tian Y H, Yin B. Ammonia volatilization and nitrogen balance after topdressing fertilization in paddy fields of Taihu Lake region. Chin J Eco-agric, 2011, 19(1): 32–36 (in Chinese with English abstract)



[28]丁艳锋, 刘胜环, 王绍华, 王强盛, 黄丕生, 凌启鸿. 氮素基、蘖肥用量对水稻氮素吸收与利用的影响. 作物学报, 2004, 30(8): 739–744



Ding Y F, Liu S H, Wang S H, Wang Q S, Huang P S, Ling Q H. Effects of the amount of basic and tillering nitrogen applied on absorption and utilization of nitrogen in rice Acta Agron Sin, 2004: 739–744 (in Chinese with English abstract)



[29]江立庚, 曹卫星. 水稻高效利用氮素的生理机制及有效途径. 中国水稻科学, 2002, 16: 261–264



Jiang L G, Cao W X. Physiological mechanism and approaches for efficient nitrogen utilization in rice. Chin J Rice Sci,2002, 16: 261–264 ( in Chinese with English abstract)



[30]Norman R, Roberts T, Slaton N, Fulford A. Nitrogen uptake efficiency of a hybrid compared with a conventional, pure-line rice cultivar. Soil Sci Am J, 2013, 77: 1235–1240



[31]陈星, 李亚娟, 刘丽, 方素萍, 方萍, 林咸永. 灌溉模式和供氮水平对水稻氮素利用效率的影响. 植物营养与肥料学报, 2012, 18: 283–290



Chen X, Li Y J, Liu L, Fang S P, Lin X Y. Effects of water management patterns and nitrogen fertilizer levels on nitrogen use efficiency of rice. Plant Nutr Fert Sci, 2012, 18: 283–290 (in Chinese with English abstract)



[32]李亚娟, 水分状况与供氮水平对水稻氮素利用效率的影响及其机制研究. 浙江大学博士学位论文, 2012



Li Y J. Studies on Effects and Mechanisms of Water Conditions and Nitrogen Fertilizer Levels on Nitrogen Use Efficiency in a Rice Cropping System. PhD Dissertation of Zhejiang University, 2012 (in Chinese with English abstract)

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