Abstract:

Wheat Spindle Streak Mosaic (WSSM) is an important virus disease mediated by soil bacterium Polynyxa graminis in China. Development and utilization of resistant cultivars is an effective way to control this disease. At present, several cultivars with WSSMV resistance have been grown in these regions, however, most resistance genes are originated from wheat species and show less diversity. Therefore, it is necessary to transfer new genes into wheat, especially from wheat relatives.
After multi-year evaluation, Chinese rye cultivar, Secale cereale cv. Jingzhouheimai, showed high and stable resistance. In order to transfer the genes into wheat, we identified the F₇ to F₉ generations of hybrid derived from Huixianhong (a susceptible wheat landrace from Henan Province, China) and Jingzhouheimai by molecular cytogenetics, molecular markers combined with disease resistance and developed five disomic addition lines (1R, 2R, R3, 5R and R7), one 5RS ditelosomic addition and two multiple addition and substitution lines with chromosome constitution 20’’+2R(2D)’’+4R’’ and 19’’+1R(1B)’’+2R(2B)’’+4R’’, respectively. The resistance identification showed that amphiploid Jinghui 1 had high and stable resistance to WSSMV, which is similar to Jingzhouheimai, indicating regular expression of rye resistance gene(s) in wheat. 2R and R7 disomic additions and 2 multiple addition and substitutions involving 2R showed similar resistance to Jinghui 1，we deduced that chromosomes 2R and R7 from Jingzhouheimai might confer WSSMV resistance. These alien chromosome lines might be useful germplasms in genetic study and breeding wheat for WSSMV resistance.

Key words: Triticum aestivum, Secale cereale ‘Jingzhouheimai’, Alien chromosome lines, Wheat spindle streak mosaic virus (WSSMV)