Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2000-2007.doi: 10.3724/SP.J.1006.2009.02000

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Application of a Functional Marker for Wide Compatibility Gene S5-n of Rice

YANG Jie,WANG Jun,CAO Qing,CHEN Zhi-De,ZHONG Wei-Gong*   

  1. Institute of Food Crops，Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center，Nanjing 210014，Jiangsu，China
  • Received:2009-01-20 Revised:2009-04-27 Online:2009-11-12 Published:2009-08-07
  • Contact: ZHONG Wei-Gong,E-mail: wgzhong0503@yahoo.com.cn; Tel: 025-84390320

PDF

3310

Cited

15

Abstract:

Wide compatibility varieties (WCVs) with S5-n are able to produce highly fertile hybrids when crossed to both indica and japonica varieties. The conventional breeding strategy of pyramiding S5-n is to test the fertility of F1 hybrid. However, this method is labour-consuming, time-consuming and. difficult in operation. The technically simplified approach of marker-assisted selection(MAS) has directly and practically used in rice breeding. Therefore, it is urgent to find a convenient and efficient way in WCV breeding with MAS. The wide compatibility gene (S5-n) has 136 bp deletion compared with the indica and japonica S-5 alleles. In order to improve the precision of marker-assisted selection for S5-n, we developed the functional marker S5136 based on the deletion in the DNA sequence of S5-n. The differentiation for S5-n, S5-i and S5-j in 24 accessions of weedy rice from the district of Yangtze and Huaihe Rivers, 27 accessions of common wild rice (O. rufipogon Griff.) from China, 554 cultivars and genetic resources (O. sativa L.) and 20 breeding lines was investigated by S5136．The results indicated that this marker was low-cost, robust technique that can be utilized to identify the S5-n in WCVs like 02428, Dular and CPSLO17. The S5136 could distinguish the homozygous S5-n/S5-n, homozygous S5-i/S5-i, and heterozygous S5-n/S5-i individuals in F2 population derived from 3037 and 02428. From the tested materials, 13 germplasms with S5-n were identified and conformed by sequencing the S5136 PCR products, and 2 restore lines with S5-n developed from the offspring of 02428 were identified. Also, the compatibility of Kasalath was tested with some indica-japonica hybrids. Therefore, S5136 is a powerful molecular marker in S5-n germplasm screening, perfect marker-assisted selection breeding and seed purity testing for the two line hybrids with Pei’ai 64S as maternal parent.

Key words: Wide compatibility gene S5-n, Functional marker, Oryza sativa L., O.rufipogen Griff., O.sativa f.spontanea

[1] ZHU Wei-Jia, WANG Rui, XUE Ying-Jie, TIAN Hong-Li, FAN Ya-Ming, WANG Lu, LI Song, XU Li, LU Bai-Shan, SHI Ya-Xing, YI Hong-Mei, LU Da-Lei, YANG Yang, WANG Feng-Ge. Development and application of functional insertion and deletion (InDel) markers associated with maize Waxy gene compatible with dual-platform [J]. Acta Agronomica Sinica, 2025, 51(9): 2330-2340.
[2] WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie. Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique [J]. Acta Agronomica Sinica, 2025, 51(9): 2547-2556.
[3] ZHANG Tian-Xing, LI Meng, WU Lin-Nan, ZHAO Hui-Xian, HU Sheng-Wu, MA Meng. Development of functional markers of wheat grain size related gene TaCYP78A17 [J]. Acta Agronomica Sinica, 2024, 50(12): 3025-3034.
[4] ZHANG Fu-Yan, CHENG Zhong-Jie, CHEN Xiao-Jie, WANG Jia-Huan, CHEN Feng, FAN Jia-Lin, ZHANG Jian-Wei, YANG Bao-An. Molecular identification and breeding application of allelic variation of grain weight gene in wheat from the Yellow-Huai-River Valley [J]. Acta Agronomica Sinica, 2021, 47(11): 2091-2098.
[5] Fang-Quan WANG,Fang-Jun FAN,Shi-Jian XIA,Shou-Yu ZONG,Tian-Qing ZHENG,Jun WANG,Wen-Qi LI,Yang XU,Zhi-Hui CHEN,Yan-Jie JIANG,Ya-Jun TAO,Wei-Gong ZHONG,Jie YANG. Interactive effects of the photoperiod-/thermo-sensitive genic male sterile genes tms5 and pms3 in rice [J]. Acta Agronomica Sinica, 2020, 46(3): 317-329.
[6] HAO Zhi-Ming,GENG Miao-Miao,WEN Shu-Min,YAN Gui-Jun,WANG Rui-Hui,LIU Gui-Ru. Development and validation of markers linked to genes resistant to Sitodiplosis mosellana in wheat [J]. Acta Agronomica Sinica, 2020, 46(02): 179-193.
[7] Wen-Jie SI,Lin-Nan WU,Li-Jian GUO,Meng-Die ZHOU,Xiang-Li LIU,Meng MA,Hui-Xian ZHAO. Development and validation of the functional marker of grain weight-related gene TaCYP78A5 in wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2019, 45(12): 1905-1911.
[8] ZHANG Hong-Juan,LI Yu-Ying,MIAO Li-Li,WANG Jing-Yi,LI Chao-Nan,YANG De-Long,MAO Xin-Guo,JING Rui-Lian. Transcription factor gene TaNAC67 involved in regulation spike length and spikelet number per spike in common wheat [J]. Acta Agronomica Sinica, 2019, 45(11): 1615-1627.
[9] Jun WANG,Jie-Yu ZHAO,Yang XU,Fang-Jun FAN,Jin-Yan ZHU,Wen-Qi LI,Fang-Quan WANG,Yun-Yan FEI,Wei-Gong ZHONG,Jie YANG. Development and Application of Functional Markers for Rice Blast Resistance Gene Bsr-d1 in Rice [J]. Acta Agronomica Sinica, 2018, 44(11): 1612-1620.
[10] Da-Wei JIAN, Yang ZHOU, Hong-Wei LIU, Li YANG, Chun-Yan MAI, Li-Qiang YU, Xin-Nian HAN, Hong-Jun ZHANG, Hong-Jie LI. Functional Markers Reveal Genetic Variations in Wheat Improved Cultivars and Landraces from Xinjiang [J]. Acta Agronomica Sinica, 2018, 44(05): 657-671.
[11] Fang-Quan WANG, Jie YANG, Fang-Jun FAN, Wen-Qi LI, Jun WANG, Yang XU, Jin-Yan ZHU, Yun-Yan FEI, Wei-Gong ZHONG. Development and Application of the Functional Marker for Imidazolinone Herbicides Resistant ALS Gene in Rice [J]. Acta Agronomica Sinica, 2018, 44(03): 324-331.
[12] ZHANG Xiao-Qiong, WANG Xiao-Wen, TIAN Wei-Jiang, ZHANG Xiao-Bo, Sun Ying, LI Yang-Yang, Xie Jia, HE Guang-Hua,SANG Xian-Chun. LAZY1 Regulates the Development of Rice Leaf Angle through BR Pathway [J]. Acta Agron Sin, 2017, 43(12): 1767-1773.
[13] ZHONG Jie,WEN Pei-Zheng,SUN Zhi-Guang,XIAO Shi-Zhuo,HU Jin-Long,ZHANG Le,JIANG Ling,CHENG Xia-Nian,LIU Yu-Qiang,WAN Jian-Min. Identification of QTLs Conferring Small Brown Planthopper Resistance in Rice (Oryza sativa L.) Using MR1523/Suyunuo F2:3 Population [J]. Acta Agron Sin, 2017, 43(11): 1596-1602.
[14] ZHOU Ke,LI Yan,WANG Shi-Ming,CUI Guo-Qing,YANG Zheng-Lin,HE Guang-Hua,LING Ying-Hua,ZHAO Fang-Ming. Identification of Rice Chromosome Segment Substitution Line Z519 with Purple Sheath and Candidate Gene Analysis of PSH1 [J]. Acta Agron Sin, 2017, 43(07): 974-982.
[15] XIAO Yan-Hua**, CHEN Xin-Long**, DU Dan, XING Ya-Di, ZHANG Tian-Quan, ZHU Mao-Di,LIU Ming-Ming,ZHU Xiao-Yan, SANG Xian-Chun,HE Guang-Hua*. Identification and Gene Mapping of Starch Accumulation and Early Senescence Leaf Mutant esl9 in Rice [J]. Acta Agron Sin, 2017, 43(04): 473-482.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd