Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1667-1676.doi: 10.3724/SP.J.1006.2014.01667

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Relationship between Leaf Photosynthetic Parameters and Drought Resistance at Silking Stage in Maize Inbred Lines

CHEN Chun-Mei,GAO Ju-Lin*,SU Zhi-Jun,YU Xiao-Fang,HU Shu-Ping,ZHAO Xiao-Liang   

  1. College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China?
  • Received:2013-12-16 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-10
  • Contact: 高聚林, E-mail: nmgaojulin@163.com E-mail:chenchunmei0224@126.com

Abstract:

 To explore drought resistance of maize inbred lines, based on leaf photosynthetic parameters at silking stage, we treated 51 maize inbred lines with drought stress at silking stage in Humochuanpingyuanguanqu and Hetaopingyuanguanqu in 2012 and 2013 to analyze response of leaf photosynthetic parameters to drought stress and the relationship between photosynthetic parameters and drought tolerance. The result indicated that at silking stage, no matter under drought and the condition of irrigation, Pn, Tr, Gs, Ci, Fv/Fm, ΦPSII, and SPAD had a significant and positive correlation with drought resistance coefficient, WUE had a significant and negative correlation with it, while qN and qP had no correlation with it. Stepwise regression analysis on the eight leaf photosynthetic parameters above showed that Pn, WUE, Fv/Fm, ΦPSII, and SPAD significantly correlated with drought resistance coefficient at P < 0.01. Pn, WUE and SPAD directly contributed to drought resistance coefficient, while Fv/Fm and ΦPSII contributed indirectly. Thirty-two maize inbred lines were classified into three groups based on different drought resistance, group 1 with the highest drought resistance contained eight inbred lines (H201, H21, Ying 64, Ji 842, Zao 49, Ji 8415, Dong 46, and Shen 137), group 2 with the higher drought resistance contained fifteen lines and group 3 with the lowest drought resistance contained nine lines. The grouping result was consistant with that of yield classification.Therefore, at silking stage, Pn, WUE, Fv/Fm, ΦPSII, SPAD, and drought resistance coefficient can be used as drought resistance evaluation indexes established for the three groups of maize inbred lines. Regression relationship was between Pn, WUE, Fv/Fm, ΦPSII, SPAD, and drought resistance coefficient at silking stage, and the threshold values were confirmed.

Key words: Maize inbred lines, silking stage, drought stress, photosynthetic parameter

[1]Campos H, Cooper M, Habben J E, Edmeades G O, Schussler J R. Improving drought tolerance in maize: a view from industry. Field Crops Res, 2004, 90: 19–34



[2]Welsh Catherine E, McMillan L. Accelerating the inbreeding of multi-parental recombinant inbred lines generated by sibling matings. Genes, Genomes, Genetics (Bethesda), 2012, 2: 191–198



[3]Henry T, Singh S P. Comparison of sources and lines selected for drought resistance in common bean. Crop Sci, 2002, 42: 64–70



[4]Lee M, Jung J H, Han D Y, Seo P J, Park W J, Park C M. Activation of a flavin monooxygenase gene YUCCA7 enhances drought resistance in Arabidopsis. Planta, 2012, 235: 925–938



[5]De Diego N, Pérez-Alfocea F, Cantero E, Lacuesta M, Moncaleán P. Physiological response to drought in radiata pine: phytohormone implication at leaf level. Tree Physiol, 2012, 32: 435–449



[6]Medrano H, Escalona J M, Bota J, Gulías J, Flexas J. Regulation of photosynthesis of C3 plants in response to progressive drought: stomatal conductance as a reference parameter. Ann Bot, 2002, 89: 895–905



[7]Saint Pierre C, Crossa J L, Bonnett D, Yamaguchi-Shinozaki K, Reynolds M P. Phenotyping transgenic wheat for drought resistance. J Exp Bot, 2012, 63: 1799–1808



[8]Deeba F, Pandey A K, Ranjan S, Mishra A, Singh R, Sharma Y K, Shirke P A, Pandey V. Physiological and proteomic responses of cotton (Gossypium herbaceum L.) to drought stress. Plant Physiol Biochem, 2012, 53: 6–18



[9]H Sjursen, Bayley M, Holmstrup M. Enhanced drought tolerance of a soil-dwelling springtail by pre-acclimation to a mild drought stress. J Insect Physiol, 2001, 47: 1021–1027



[10]Aroca R, Irigoyen J J, Sánchez-díaz M. Drought enhances maize chilling tolerance: II. Photosynthetic traits and protective mechanisms against oxidative stress. Physiol Plant, 2003, 117: 540–549



[11]Baker N R, Rosenqvist E. Application of chlorophyll fluorescencecan improve crop production strategies: an examination of future possibilities. J Exp Bot, 2004, 55: 1607–1621



[12]Schreiber U, Schliwa U, Bilger W. Continuous recording of photochemical and non photochemical chlorophyll fluorescence equenching with a new type of modulation fluorometer. Photosynth Res, 1986, 10: 51–62



[13]Selmani A, Wasson C E. Daytime chlorophyll fluorescence measurement in field-grown maize and its genetic variability under well-water and water-stressed conditions. Field Crops Res, 2003, 31: 173–184



[14]胡秀丽, 李艳辉, 杨海荣, 刘全军, 李潮海. HSP70可提高干旱高温复合胁迫诱导的玉米叶片抗氧化防护能力. 作物学报, 2010, 36: 636–644



Hu X L, Li Y H, Yang H R, Liu Q J, Li C H. Heat shock protein 70 may improve the ability of antioxidant defense induced by the combination of drought and heat in maize leaves. Acta Agron Sin, 2010, 36: 636–644 (in Chinese with English abstract)



[15]Hsiao. Plant responses to water stress. Annu Rev Plant Physical, 1973, 24: 570–579



[16]Harrigan G G, Ridley W P, Miller K D, Sorbet R, Riordan S G, Nemeth M A, Reeves W, Pester T A. The forage and grain of MON 87460, a drought-tolerant corn hybrid, are compositionally equivalent to that of conventional corn. J Agric Food Chem, 2009, 57: 9754–9763



[17]张守仁. 叶绿素荧光动力学参数的意义及讨论. 植物学通报, 1999, 16: 444–448



Zhang S R. A discussion of chlorophyll fluorescence kinetics parameter and their significance. Chin Bull Bot, 1999, 16: 444–448 (in Chinese)



[18]Lu Y L, Hao Z F, Xie C X. Large-scale screening for maize drought resistance using multiple selection criteria evaluated under water-stressed and well-watered environments. Field Crops Res, 2011, 124: 37–45



[19]许大全. 光合作用效率. 上海: 上海科学技术出版社, 2009



Xu D Q. Photosynthetic Efficiency. Shanghai: Shanghai Scientific and Technical Publishers, 2009 (in Chinese)



[20]Harp R E, Poroyko V, Hejlek L G, Spollen W G, Springer G K, Bohnert H J, Nguyen H T. Root growth maintenance during water deficits: physiology to functional genomies. J Exp Bot, 2004, 55: 2343–2351



[21]陈建明, 俞晓平, 程家安. 叶绿素荧光动力学及其在植物抗逆生理研究中的应用. 浙江农业学报, 2006, 18(1): 51–55



Chen J M, Yu X P, Cheng J A. The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Acta Agric Zhejiangensis, 2006, 18(1): 51–55 (in Chinese)



[22]白向历, 孙世贤, 杨国航, 刘明, 张振平, 齐华. 不同生育时期水分胁迫对玉米产量及生长发育的影响. 玉米科学, 2009, 17(2): 60–63



Bai X L, Sun S X, Yang G H, Liu M, Zhang Z P, Qi H. Effect of water stress on maize yield during different growing stages. J Maize Sci, 2009, 17(2): 60–63(in Chinese with English abstract)



[23]孙璐, 周宇飞, 汪澈, 肖木辑, 陶冶, 许文娟, 黄瑞冬.高粱品种萌发期耐盐性筛选与鉴定. 中国农业科学, 2012,45: 1714–1722



Sun L, Zhou Y F, Wang C, Xiao M J, Tao Y, Xu W J, Huang R D. Screening and identification of sorghum cultivars for salinity tolerance during germination. Sci Agric Sin, 2012, 45: 1714–1722 (in Chinese with English abstract)



[24]Baquedano F J, Castillo F J. Comparative ecophysiological effects of drought on seedlings of the Mediterranean water-saver Pinus halepensis and water-spenders Quercus coccifera and Quercus ilex. Trees, 2006, 20: 689–700



[25]付学琴, 贺浩华, 文飘, 罗向东, 谢建坤. 东乡野生稻回交重组系的抗旱性评价体系. 应用生态学报, 2012, 23: 1277–1285



Fu X Q, He H H, Wen P, Luo X D, Xie J K. Drought resistance evaluation system for backcross lines of Dongxiang common wild rice (Oryza rufipogon Griff.). Chin J Appl Ecol, 2012, 23: 1277–1285 (in Chinese with English abstract)



[26]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000



Li H S. Experimental Principle and Technique for Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000 (in Chinese)



[27]Bouslama M, Schapaugh W T Jr. Stress tolerance in soybeans: I. Evaluation of three screening techniques for heat and drought tolerance. Crop Sci, 1984, 24: 933–937



[28]龚明. 作物抗旱性鉴定方法与指标及其综合评价. 云南农业大学学报, 1989, 4(1): 73–81



Gong M. Screening methods and index of drought resistance in crops and comprehensive evaluation. J Yunnan Agric Univ, 1989, 4(11): 73–81 (in Chinese)



[29]Pollard Daniel A. Design and construction of recombinant inbred lines. Methods Mol Biol, 2012, 871: 31–39

[1] Yang Biao, Du Shuai-Kang, Zhang Ji-Wang, Shi Ying, Zhang Li-Li. Genome-wide identification of class III POD gene family in potato and its expression profile analysis [J]. Acta Agronomica Sinica, 2026, 52(2): 405-420.
[2] Wang Ya-Zhi, Yang Biao, Ji Xiang-Lin, Shi Ying, Zhang Li-Li. Identification of drought-resistant resources and preliminary screening of drought resistant genes in diploid potatoes [J]. Acta Agronomica Sinica, 2026, 52(1): 72-84.
[3] Kong Na, Liu Tao, Liu Wen-Ting, Chen Gang, Wen Li-Chao, Deng Zhi-Chao, Guo Mei, Li Wei, Guo Yong-Feng. Cloning of the NtCEP7 gene in tobacco and functional analysis of its encoded peptide in seedling-stage drought resistance [J]. Acta Agronomica Sinica, 2026, 52(1): 249-261.
[4] Liu Hai-Bo, Zhang Lei, Wang Li-Qi, Shi Xiao-Li, Zhou Wen-Ying, Cui Guo-Xian, She Wei. Functional study of the BnGCL1 gene in ramie (Boehmeria nivea L.) in response to drought stress [J]. Acta Agronomica Sinica, 2026, 52(1): 14-27.
[5] Hu Cheng-Zhen, Gao Wei-Dong, Kong Bin-Xue, Wang Jian-Fei, Che Zhuo, Yang De-Long, Chen Tao. Genome-wide identification of the TaAPC11 gene family in wheat and functional characterization of TaAPC11-5B in drought stress responses [J]. Acta Agronomica Sinica, 2026, 52(1): 148-164.
[6] HE Peng-Xu, YAO Li-Rong, CHEN Yuan-Ling, YAN Yan, ZHANG Hong, WANG Jun-Cheng, LI Bao-Chun, YANG Ke, SI Er-Jing, MENG Ya-Xiong, MA Xiao-Le, WANG Hua-Jun. Differences and correlations in physiological and molecular mechanisms of barley germination under drought stress [J]. Acta Agronomica Sinica, 2025, 51(9): 2412-2432.
[7] LU Wen-Jia, WANG Jun-Cheng, YAO Li-Rong, ZHANG Hong, SI Er-Jing, YANG Ke, MENG Ya-Xiong, LI Bao-Chun, MA Xiao-Le, WANG Hua-Jun. Genome-wide identification of PRX gene family and analysis of their expressions under drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(5): 1198-1214.
[8] WANG Lin, CHEN Xiao-Yu, ZHANG Wen-Meng-Long, WANG Si-Qi, CHENG Bing-Yun, CHENG Jing-Qiu, PAN Rui, ZHANG Wen-Ying. Molecular characteristics and functional analysis of HvMYB2 in response to drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(4): 873-887.
[9] HUO Ru-Xue, GE Xiang-Han, SHI Jia, LI Xue-Rui, DAI Sheng-Jie, LIU Zhen-Ning, LI Zong-Yun. Functional analysis of the sweetpotato histidine kinase protein IbHK5 in response to drought and salt stresses [J]. Acta Agronomica Sinica, 2025, 51(3): 650-666.
[10] MA Qun, WANG Zhi-Hao, YAN Lei, LI Yu-Jiao, WANG Jia-Qi, LI Zhao, LIU Wei, AI Xin, MA Qian-Chi, WANG Xiao-Guang, ZHONG Chao, REN Jing-Yao, LIU Xi-Bo, ZHAO Shu-Li, ZHANG He, ZHAO Xin-Hua, JIANG Chun-Ji, WANG Jing, YU Hai-Qiu. Screening and evaluation system for drought resistance in high-oleic acid and common peanut at the germination stage [J]. Acta Agronomica Sinica, 2025, 51(12): 3266-3280.
[11] WEI Qi, HE Guan-Hua, ZHANG Deng-Feng, LI Yong-Xiang, LIU Xu-Yang, TANG Huai-Jun, LIU Cheng, WANG Tian-Yu, LI Yu, LU Yun-Cai, LI Chun-Hui. Identifying of excellent drought-tolerant gene resources based on drought- tolerant maize inbred line SL001 [J]. Acta Agronomica Sinica, 2025, 51(12): 3171-3183.
[12] LIU Yong-Hui, SHEN Yi, SHEN Yue, LIANG Man, SHA Qin, ZHANG Xu-Yao, CHEN Zhi-De. Cloning and functional analysis of drought-inducible promoter AhMYB44-11- Pro in peanut (Arachis hypogaea L.) [J]. Acta Agronomica Sinica, 2024, 50(9): 2157-2166.
[13] LI Wen-Juan, WANG Li-Min, QI Yan-Ni, ZHAO Wei, XIE Ya-Ping, DANG Zhao, ZHAO Li-Rong, LI Wen, XU Chen-Meng, WANG Yan, ZHANG Jian-Ping. Functional analysis of flax LuWRI1a in response to drought and salt stresses [J]. Acta Agronomica Sinica, 2024, 50(7): 1750-1761.
[14] QIAO Zhi-Xin, ZHANG Jie-Dao, WANG Yu, GUO Qi-Fang, LIU Yan-Jing, CHEN Rui, HU Wen-Hao, SUN Ai-Qing. Difference in germination characteristics of different winter wheat cultivars under drought stress [J]. Acta Agronomica Sinica, 2024, 50(6): 1568-1583.
[15] WANG Li-Ping, WANG Xiao-Yu, FU Jing-Ye, WANG Qiang. Functional identification of maize transcription factor ZmMYB12 to enhance drought resistance and low phosphorus tolerance in plants [J]. Acta Agronomica Sinica, 2024, 50(1): 76-88.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!