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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (02): 306-312.doi: 10.3724/SP.J.1006.2018.00306

• Orginal Article • Previous Articles    

Analysis of Brown Rust Resistance Inheritance Based on Field Phenotypes and Detection of Bru1 Gene in Sugarcane

Zhu LI, Li-Ping XU*(), Ya-Chun SU, Qi-Bin WU, Wei CHENG, Ting-Ting SUN, Shi-Wu GAO   

  1. Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture / Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;
  • Received:2017-04-13 Accepted:2017-11-21 Online:2018-02-12 Published:2017-12-04
  • Contact: Li-Ping XU E-mail:xlpmail@126.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31571732) and the China Agriculture Research System (CARS-20).

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Abstract:

With characteristics of high pathogenic spore yield and strong popularity, sugarcane brown rust caused by Puccinia melanocephala leads to a serious loss in sugarcane yield and sucrose content. An understanding of the inheritance of brown rust resistance will contribute to parent selections and combination arrangements, while an efficient technique for resistance identification is necessary for selection of segregated individuals. Combination phenotypic survey with Bru1 gene detection, the segregated population derived from crossing combinations with different brown rust resistances was used to explore rust resistance inheritance and evaluate the efficiency of Bru1 gene detection. The undetected rate of Bru1 gene in the individuals with rust sensitivity at the stage of prosperous epidemic peak was 96.7%, while the detected rate in all uninfected individuals was only 66.0% in spite of two from four combinations showed 100.0% in resistant individuals. It indicates the disease sensitivity and resistance can be estimated based on the detection of Bru1 gene in the genetic background with rust resistance regulated by Bru1. In addition, none of the healthy individuals in the segregated population derived from combination of CP84-1198 × Yunzhe 89-7 have Bru1 gene been detected, suggesting the new resistance genes, different from Bru1, are present in sugarcane gene pool. In addition, both maternal and paternal components affected the rust susceptibility of their offspring, but male parent other than female parent tended to transmit a higher frequency of rust-susceptible genes.

Key words: sugarcane, brown rust, segregation population, inheritance of disease resistance, Bru1 gene, incidence rate

Table 1

Identification standard of sugarcane resistance to brown rust disease"

分级标准
Grade		 抗病性
Resistance		 叶片侵染状况
Leaf infection performance
1 高抗
High resistance		 无症状。
No symptoms.
2 抗病
Resistance		 有坏死斑, 病斑占叶面积10%以下。
The area of necrotic spots on leaves is lower than 10%.
3
 中抗
Moderate resistance		 植株上有一些孢子堆, 病斑占叶面积11%~25%。
There are piles of spores visible on the plant. The percentages of lesion spots’ area to leaf area are 11%-25%.
4
 中感
Moderate susceptibility		 上层1~3片叶有一些孢子堆, 同时下层叶有许多孢子堆, 病斑占叶面积26%~35%。
There are some spore piles from leaf 1 to leaf 3 at the top and many spores on lower leaves. The percentages of lesion spots’ area in leaf are 26%-35%.
5
 感病1
Susceptibility 1		 上层1~3片叶有极多孢子堆, 同时下层叶有轻微的坏死, 病斑占叶面积36%~50%。
There are abundant spore piles from leaf 1 to leaf 3 at the top and slight necrotic spots on lower leaves. The percentages of lesion spots’ area in leaf are 36%-50%.
6
 感病2
Susceptibility 2		 上层1~3片叶有极多孢子堆且下层叶有比第5级更多的坏死, 病斑占叶面积51%~60%。
There are abundant spore piles from leaf 1 to leaf 3 at the top and more necrotic spots on lower leaves than those at grade 5. The percentages of lesion spots’ area in leaf are 51%-60%.
7
 感病3
Susceptibility 3		 上层1~3片叶有极多孢子堆, 下层叶坏死, 病斑占叶面积61%~75%。
There are abundant spore piles from leaf 1 to leaf 3 at the top and necrotic spots on lower leaves. The percentages of lesion spots’ area in leaf are 61%-75%.
8
 高感1
High susceptibility 1		 上层1~3片叶有某些坏死, 病斑占叶面积76%~90%。
There are some necrotic spots from leaf 1 to leaf 3 at the top. The percentages of lesion spots’ area in leaf are 76%-90%.
9
 高感2
High susceptibility 2		 叶片坏死, 植株濒于死亡, 病斑占叶面积91%~100%。
Leaves are necrotic and plants are almost dead. The percentages of lesion spots’ area in leaf are 91%-100%.

Table 2

Identification results of brown rust resistance, Bru1 gene and incidence rate in sugarcane crossing parents and their segregation population"

杂交组合
Hybrid combination
(♀ × ♂)		 母本抗性等级/ Bru1检出结果
Resistance rank for female parent/ detected Bru1		 父本抗性等级/ Bru1检出结果
Resistance rank for male parent/ detected Bru1		 分离群体在发病盛期的
褐锈病发生率
Incidence rate in segregation population at the peak (%)
ROC251) × 柳城03-11372)
ROC25 × Liucheng 03-1137		 高抗/ Y
High resistance/ Y		 感病2/ N
Susceptibility 2/ N		 39.0
ROC25 × 粤糖84-31)
ROC25 × Yuetang 84-3		 高抗/ Y
High resistance/ Y		 高感2/ N
High susceptibility 2/ N		 54.3
CP94-11001)× ROC25 感病1/ N
Susceptibility 1/ N		 高抗/ Y
High resistance/ Y		 28.4
CP84-11981) × 云蔗89-71)
CP84-1198 × Yunzhe 89-7		 高抗/ N
High resistance/ N		 高抗/ N
High resistance/ N		 13.0

Fig. 1

Detection of Bru1 gene in sugarcane brown rust resistant and susceptible control varieties based on the markers of R12H16 (A) and 9O20-F4 (B) M: 100 bp DNA ladder marker; R: Brown rust resistant control variety R570; S: Brown rust susceptible control variety YT60; CK: Blank control."

Table 3

Brown rust resistance and detection of Bru1 in segregation population derived from four sugarcane hybrid combinations"

杂交组合
Hybrid combination		 样品数
Sample number		 发病盛期田间调查
Field investigation at epidemic peak stage		 发病末期田间调查
Field investigation at epidemic late stage
未发病率
Uninfected
rate (%)		 Bru1检出率
Bru1
detected rate (%)		 R12H16和9O20-F4标记检出一致性DCR9MP 未发病率
Uninfected
rate (%)		 Bru1检出率
Bru1 detected rate (%)		 R12H16和9O20-F4标记检出一致性DCR9M
ROC25×柳城03-1137
ROC25×Liucheng 03-1137		 15 0 0 一致Consistent 0 0 一致Consistent
15 100.0 100.0 一致Consistent 86.7 100.0 一致Consistent
ROC25×粤糖84-3
ROC25×Yuetang 84-3		 15 0 13.3 一致Consistent 13.3 13.3 一致Consistent
15 100.0 100.0 一致Consistent 60.0 100.0 一致Consistent
CP94-1100×ROC25 15 0 0 一致Consistent 6.7 0 一致Consistent
15 100.0 80.0 一致Consistent 93.3 80.0 一致Consistent
CP84-1198×云蔗89-7
CP84-1198×Yunzhe 89-7		 15 0 0 一致Consistent 0 0 一致Consistent
15 100.0 0 一致Consistent 93.3 0 一致Consistent

Fig. 2

Detection of resistance gene Bru1 with R12H16 and 9O20-F4 markers in susceptible plants of hybrid combination ROC25×Yuetang 84-3 M: DNA marker; Lanes 1-15: susceptible plants; PC: resistance control ROC16; NC: susceptible control FN39; CK: blank control."

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