Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (02): 306-312.doi: 10.3724/SP.J.1006.2018.00306

• Orginal Article • Previous Articles    

Analysis of Brown Rust Resistance Inheritance Based on Field Phenotypes and Detection of Bru1 Gene in Sugarcane

Zhu LI, Li-Ping XU*(), Ya-Chun SU, Qi-Bin WU, Wei CHENG, Ting-Ting SUN, Shi-Wu GAO   

  1. Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture / Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;
  • Received:2017-04-13 Accepted:2017-11-21 Online:2018-02-12 Published:2017-12-04
  • Contact: Li-Ping XU E-mail:xlpmail@126.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31571732) and the China Agriculture Research System (CARS-20).

RichHTML319

1

PDF

739

Abstract:

With characteristics of high pathogenic spore yield and strong popularity, sugarcane brown rust caused by Puccinia melanocephala leads to a serious loss in sugarcane yield and sucrose content. An understanding of the inheritance of brown rust resistance will contribute to parent selections and combination arrangements, while an efficient technique for resistance identification is necessary for selection of segregated individuals. Combination phenotypic survey with Bru1 gene detection, the segregated population derived from crossing combinations with different brown rust resistances was used to explore rust resistance inheritance and evaluate the efficiency of Bru1 gene detection. The undetected rate of Bru1 gene in the individuals with rust sensitivity at the stage of prosperous epidemic peak was 96.7%, while the detected rate in all uninfected individuals was only 66.0% in spite of two from four combinations showed 100.0% in resistant individuals. It indicates the disease sensitivity and resistance can be estimated based on the detection of Bru1 gene in the genetic background with rust resistance regulated by Bru1. In addition, none of the healthy individuals in the segregated population derived from combination of CP84-1198 × Yunzhe 89-7 have Bru1 gene been detected, suggesting the new resistance genes, different from Bru1, are present in sugarcane gene pool. In addition, both maternal and paternal components affected the rust susceptibility of their offspring, but male parent other than female parent tended to transmit a higher frequency of rust-susceptible genes.

Key words: sugarcane, brown rust, segregation population, inheritance of disease resistance, Bru1 gene, incidence rate

Table 1

Identification standard of sugarcane resistance to brown rust disease"

分级标准
Grade		 抗病性
Resistance		 叶片侵染状况
Leaf infection performance
1 高抗
High resistance		 无症状。
No symptoms.
2 抗病
Resistance		 有坏死斑, 病斑占叶面积10%以下。
The area of necrotic spots on leaves is lower than 10%.
3
 中抗
Moderate resistance		 植株上有一些孢子堆, 病斑占叶面积11%~25%。
There are piles of spores visible on the plant. The percentages of lesion spots’ area to leaf area are 11%-25%.
4
 中感
Moderate susceptibility		 上层1~3片叶有一些孢子堆, 同时下层叶有许多孢子堆, 病斑占叶面积26%~35%。
There are some spore piles from leaf 1 to leaf 3 at the top and many spores on lower leaves. The percentages of lesion spots’ area in leaf are 26%-35%.
5
 感病1
Susceptibility 1		 上层1~3片叶有极多孢子堆, 同时下层叶有轻微的坏死, 病斑占叶面积36%~50%。
There are abundant spore piles from leaf 1 to leaf 3 at the top and slight necrotic spots on lower leaves. The percentages of lesion spots’ area in leaf are 36%-50%.
6
 感病2
Susceptibility 2		 上层1~3片叶有极多孢子堆且下层叶有比第5级更多的坏死, 病斑占叶面积51%~60%。
There are abundant spore piles from leaf 1 to leaf 3 at the top and more necrotic spots on lower leaves than those at grade 5. The percentages of lesion spots’ area in leaf are 51%-60%.
7
 感病3
Susceptibility 3		 上层1~3片叶有极多孢子堆, 下层叶坏死, 病斑占叶面积61%~75%。
There are abundant spore piles from leaf 1 to leaf 3 at the top and necrotic spots on lower leaves. The percentages of lesion spots’ area in leaf are 61%-75%.
8
 高感1
High susceptibility 1		 上层1~3片叶有某些坏死, 病斑占叶面积76%~90%。
There are some necrotic spots from leaf 1 to leaf 3 at the top. The percentages of lesion spots’ area in leaf are 76%-90%.
9
 高感2
High susceptibility 2		 叶片坏死, 植株濒于死亡, 病斑占叶面积91%~100%。
Leaves are necrotic and plants are almost dead. The percentages of lesion spots’ area in leaf are 91%-100%.

Table 2

Identification results of brown rust resistance, Bru1 gene and incidence rate in sugarcane crossing parents and their segregation population"

杂交组合
Hybrid combination
(♀ × ♂)		 母本抗性等级/ Bru1检出结果
Resistance rank for female parent/ detected Bru1		 父本抗性等级/ Bru1检出结果
Resistance rank for male parent/ detected Bru1		 分离群体在发病盛期的
褐锈病发生率
Incidence rate in segregation population at the peak (%)
ROC251) × 柳城03-11372)
ROC25 × Liucheng 03-1137		 高抗/ Y
High resistance/ Y		 感病2/ N
Susceptibility 2/ N		 39.0
ROC25 × 粤糖84-31)
ROC25 × Yuetang 84-3		 高抗/ Y
High resistance/ Y		 高感2/ N
High susceptibility 2/ N		 54.3
CP94-11001)× ROC25 感病1/ N
Susceptibility 1/ N		 高抗/ Y
High resistance/ Y		 28.4
CP84-11981) × 云蔗89-71)
CP84-1198 × Yunzhe 89-7		 高抗/ N
High resistance/ N		 高抗/ N
High resistance/ N		 13.0

Fig. 1

Detection of Bru1 gene in sugarcane brown rust resistant and susceptible control varieties based on the markers of R12H16 (A) and 9O20-F4 (B) M: 100 bp DNA ladder marker; R: Brown rust resistant control variety R570; S: Brown rust susceptible control variety YT60; CK: Blank control."

Table 3

Brown rust resistance and detection of Bru1 in segregation population derived from four sugarcane hybrid combinations"

杂交组合
Hybrid combination		 样品数
Sample number		 发病盛期田间调查
Field investigation at epidemic peak stage		 发病末期田间调查
Field investigation at epidemic late stage
未发病率
Uninfected
rate (%)		 Bru1检出率
Bru1
detected rate (%)		 R12H16和9O20-F4标记检出一致性DCR9MP 未发病率
Uninfected
rate (%)		 Bru1检出率
Bru1 detected rate (%)		 R12H16和9O20-F4标记检出一致性DCR9M
ROC25×柳城03-1137
ROC25×Liucheng 03-1137		 15 0 0 一致Consistent 0 0 一致Consistent
15 100.0 100.0 一致Consistent 86.7 100.0 一致Consistent
ROC25×粤糖84-3
ROC25×Yuetang 84-3		 15 0 13.3 一致Consistent 13.3 13.3 一致Consistent
15 100.0 100.0 一致Consistent 60.0 100.0 一致Consistent
CP94-1100×ROC25 15 0 0 一致Consistent 6.7 0 一致Consistent
15 100.0 80.0 一致Consistent 93.3 80.0 一致Consistent
CP84-1198×云蔗89-7
CP84-1198×Yunzhe 89-7		 15 0 0 一致Consistent 0 0 一致Consistent
15 100.0 0 一致Consistent 93.3 0 一致Consistent

Fig. 2

Detection of resistance gene Bru1 with R12H16 and 9O20-F4 markers in susceptible plants of hybrid combination ROC25×Yuetang 84-3 M: DNA marker; Lanes 1-15: susceptible plants; PC: resistance control ROC16; NC: susceptible control FN39; CK: blank control."

[1] Avellaneda M C, Hoy J W, Pontif M J.Screening for resistance to sugarcane brown rust with controlled-conditions inoculation. Plant Dis, 2015, 99: 1633-1639
[2] 黄鸿能. 警惕甘蔗锈病在蔗区蔓延. 甘蔗糖业, 1991, (6): 17-19
Huang H N.Maintain vigilance for the spread of rust in sugarcane area. Sugar Canes, 1991, (6): 17-19 (in Chinese with English abstract)
[3] Bernard F A.Considerations of the appearance of sugarcane rust disease in the Dominican Republic. Plant Pathol, 1980, 17: 1382-1386
[4] Purdy L H, Liu L J, Dean J L.Sugarcane rust: a newly important disease.Plant Dis, 1983, 11: 1292-1296
[5] Comstock J C, Shine J M, Raid R N.Effect of rust on sugarcane growth and biomass. Plant Dis, 1992, 76: 175-177
[6] Comstock J C, Shine J M, Raid R N.Effect of early rust infection on subsequent sugarcane growth. Sugar Canes, 1992, 4: 7-9
[7] 李文凤, 王晓燕, 黄应昆, 张荣跃, 单红丽, 尹炯, 申科, 罗志明. 甘蔗抗褐锈病基因Bru1分子检测体系的建立与应用. 植物保护, 2015, 41(02): 120-124
Li W F, Wang X Y, Huang Y K, Zhang R Y, Shan H L, Yin J, Shen K, Lou Z M.Establishment and application of molecular detection system for sugarcane rust resistance gene Bru1. Plant Prot, 2015, 41(02): 120-124 (in Chinese with English abstract)
[8] Daugrois J H, Grivet L, Roques D, Hoarau J Y, Lombard H, Glaszmann J C, D’Hont A. A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar ‘R570’.Theor Appl Genet, 1996, 92: 1059-1064
[9] Asnaghi C, Roques D, Ruffel S, Kaye C, Hoarau J Y, lismart H T, Girard J C, Raboin L M, Risterucci A M, Grivet L, D’Hont A. Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers. Theor Appl Genet, 2004, 108: 759-764
[10] Cunff L L, Garsmeur O, Raboin L M, Pauquet J, Telismart H, Selvi A, Grivet L, Philippe R, Begum D, Deu M, Costet L, Wing R, Glaszmann J C, D’Hont A. Diploid / polyploid syntenic shuttle mapping and haplotype-specific chromosome walking toward a rust resistance gene (Bru1) in highly polyploid sugarcane (2n approximately 12× approximately 115). Genetics, 2008, 180: 649-660
[11] Glynn N C, Laborde C, Davidson R W, Irey M S, Glaz B, D’Hont A, Comstock J C. Utilization of a major brown rust resistance gene in sugarcane breeding. Mol Breed, 2013, 31: 323-331
[12] Parco A S, Avellaneda M C, Hale A, Hoy J W, Kimbeng C A, Pontif M J, Gravois K A, Baisakh N.Frequency and distribution of the brown rust resistance geneBru1 and implications for the Louisiana sugarcane breeding programme. Plant Breed, 2014, 133: 654-659
[13] 许莉萍, 陈如凯. 甘蔗锈病抗性指标及甘蔗无性系的抗性评价. 福建农业大学学报, 1996, 25(02): 128-131
Xu L P, Chen R K.The assessment of rust-resistant indexes and rust-resistance in sugarcane clones.J Fujian Agric Univ, 1996, 25(02): 128-131 (in Chinese with English abstract)
[14] 李文凤, 王晓燕, 黄应昆, 张荣跃, 单红丽, 尹炯, 罗志明. 31份甘蔗野生核心种质资源褐锈病抗性鉴定及Bru1基因的分子检测. 作物学报, 2015, 41: 806-812
Li W F, Wang X Y, Huang Y K, Zhang R Y, Shan H L, Yin J, Luo Z M.Identification of brown rust resistance and molecular detection ofBru1 gene in 31 sugarcane wild core germplasm resources. Acta Agron Sin, 2015, 41: 806-812 (in Chinese with English abstract)
[15] Li W F, Wang X Y, Huang Y K, Zhang H Y, Shan H L, Yin J, Luo Z M.Molecular detection ofBru1 gene and identification of brown rust resistance in Chinese sugarcane germplasm. Sugar Tech, 2016, 19: 183-190
[16] Racedo J, Perera M F, Bertani R, Funes C, Gonzalez V, Cuenya M I, D’Hont A, Welin B, Castagnaro A P.Bru1 gene and potential alternative sources of resistance to sugarcane brown rust disease. Euphytica, 2013, 191: 429-436
[17] Tai P Y P, Miller J D, Dean J L. Inheritance of resistance to rust in sugarcane. Field Crops Res, 1981, 4: 261-268
[18] Hogarth D M, Ryan C C, Taylor P W J. Quantitative inheritance of rust resistance in sugarcane.Field Crops Res, 1993, 34: 187-193
[19] 王建南, 林彦铨, 邓祖湖, 陈如凯. 甘蔗对锈病的抗性遗传与育种策略: I. F1群体对锈病抗性的分析. 福建农业大学学报, 1994, 23: 140-144
Wang J N, Lin Y Q, Deng Z H, Chen R K.Inheritance and breeding strategies of resistance to rust in sugarcane: I. An analysis of the resistance to rust of F1 population.J Fujian Agric Univ, 1994, 23: 140-144 (in Chinese with English abstract)
[20] 邓祖湖, 林彦铨. 甘蔗对锈病的抗性遗性与育种策略: II. 亲本组合抗锈病的配合力. 福建农业大学学报, 1994, 23: 249-252
Deng Z H, Lin Y Q.Inheritance and breeding strategies of resistance to rust in sugarcane: II. Combining ability of parental combinations for resistance to rust.J Fujian Agric Univ, 1994, 23: 249-252 (in Chinese with English abstract)
[21] Costet L, Le C L, Royaert S, Raboin L M, Hervouet C, Toubi L, Telismart H, Garsmeur O, Rousselle Y, Pauquet J, Nibouche S, Glaszmann J C, Hoarau J Y, D’Hont A. Haplotype structure aroundBru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars. Theor Appl Genet, 2012, 125: 825-836
[22] Aljanabi S M, Parmessur Y, Moutia Y, Saumtally S, Dookun A.Further evidence of the association of a phytoplasma and a virus with yellow leaf syndrome in sugarcane.Plant Pathol, 2001, 50: 628-636
[23] 傅华英, 肖胜华, 刘营航, 孙生仁, 吴小斌, 陈如凯, 高三基. 我国甘蔗锈病病原菌及甘蔗品系抗褐锈病基因Bru1的分子检测. 热带作物学报, 2016, 37: 958-963
Fu H Y, Xiao S H, Liu Y H, Sun S R, Wu X B, Chen R K, Gao S J.Molecular detection of causal pathogens causing rust disease andBru1 resistance gene in elite sugarcane clones. Chin J Trop Crop, 2016, 37: 958-963 (in Chinese with English abstract)
[24] Asnaghi C, D’ Hont A, Glaszmann J C, Rott P.Resistance of sugarcane cultivar R570 toPuccinia melanocephala isolates from different geographic locations. Plant Dis, 2001, 85: 282-286
[25] Mcintyre C L, Whan V A, Croft B, Magarey R, Smith G R.Identification and validation of molecular markers associated with pachymetra root rot and brown rust resistance in sugarcane using map- and association-based approaches.Mol Breed, 2005, 16: 151-161
[26] Molina B L, Rosales-Longo F, Queme J L.Comparative analysis between phenotype and Bru1 marker for incidence to brown rust in sugarcane. XXVIII ISSCT Congress, 2013, 28: 1-4
[27] 李文凤, 王晓燕, 黄应昆, 张荣跃, 单红丽, 罗志明, 尹炯. 101份中国甘蔗主要育种亲本褐锈病抗性鉴定及Bru1基因的分子检测. 作物学报, 2016, 42: 1411-1416
Li W F, Wang X Y, Huang Y K, Zhang R Y, Shan L H, Luo Z M, Yin J.Identification of resistance to brown rust and molecular detection ofBru1 gene in 101 main sugarcane breeding parents in China. Acta Agron Sin, 2016, 42: 1411-1416 (in Chinese with English abstract)
[28] Zhang J, Sharma A, Yu Q Y, Wang J P, Li L T, Zhu L, Zhang X T, Chen Y Q, Ming R.Comparative structural analysis ofBru1, region homeologs in Saccharum spontaneum, and S. officinarum. BMC Genomics, 2016, 17: 1-20
[29] Glynn N.Screening for resistance to brown rust of sugarcane: use ofBru1 resistance gene prospects and challenges. J Am Soc Sugar Canes Technol, 2012, 32: 82
[1] WANG Jing-Tian, ZHANG Ya-Wen, DU Ying-Wen, REN Wen-Long, LI Hong-Fu, SUN Wen-Xian, GE Chao, ZHANG Yuan-Ming. SEA v2.0: an R software package for mixed major genes plus polygenes inheritance analysis of quantitative traits [J]. Acta Agronomica Sinica, 2022, 48(6): 1416-1424.
[2] XIAO Jian, CHEN Si-Yu, SUN Yan, YANG Shang-Dong, TAN Hong-Wei. Characteristics of endophytic bacterial community structure in roots of sugarcane under different fertilizer applications [J]. Acta Agronomica Sinica, 2022, 48(5): 1222-1234.
[3] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[4] KONG Chui-Bao, PANG Zi-Qin, ZHANG Cai-Fang, LIU Qiang, HU Chao-Hua, XIAO Yi-Jie, YUAN Zhao-Nian. Effects of arbuscular mycorrhizal fungi on sugarcane growth and nutrient- related gene co-expression network under different fertilization levels [J]. Acta Agronomica Sinica, 2022, 48(4): 860-872.
[5] YANG Zong-Tao, LIU Shu-Xian, CHENG Guang-Yuan, ZHANG Hai, ZHOU Ying-Shuan, SHANG He-Yang, HUANG Guo-Qiang, XU Jing-Sheng. Sugarcane ubiquitin-like protein UBL5 responses to SCMV infection and interacts with SCMV-6K2 [J]. Acta Agronomica Sinica, 2022, 48(2): 332-341.
[6] ZHANG Hai, CHENG Guang-Yuan, YANG Zong-Tao, LIU Shu-Xian, SHANG He-Yang, HUANG Guo-Qiang, XU Jing-Sheng. Sugarcane PsbR subunit response to SCMV infection and its interaction with SCMV-6K2 [J]. Acta Agronomica Sinica, 2021, 47(8): 1522-1530.
[7] SU Ya-Chun, LI Cong-Na, SU Wei-Hua, YOU Chui-Huai, CEN Guang-Li, ZHANG Chang, REN Yong-Juan, QUE You-Xiong. Identification of thaumatin-like protein family in Saccharum spontaneum and functional analysis of its homologous gene in sugarcane cultivar [J]. Acta Agronomica Sinica, 2021, 47(7): 1275-1296.
[8] WANG Heng-Bo, CHEN Shu-Qi, GUO Jin-Long, QUE You-Xiong. Molecular detection of G1 marker for orange rust resistance and analysis of candidate resistance WAK gene in sugarcane [J]. Acta Agronomica Sinica, 2021, 47(4): 577-586.
[9] ZHANG Rong-Yue, WANG Xiao-Yan, YANG Kun, SHAN Hong-Li, CANG Xiao-Yan, LI Jie, WANG Chang-Mi, YIN Jiong, LUO Zhi-Ming, LI Wen-Feng, HUANG Ying-Kun. Identification of brown rust resistance and molecular detection of Bru1 gene in new and main cultivated sugarcane varieties [J]. Acta Agronomica Sinica, 2021, 47(2): 376-382.
[10] CANG Xiao-Yan, XIA Hong-Ming, LI Wen-Feng, WANG Xiao-Yan, SHAN Hong-Li, WANG Chang-Mi, LI Jie, ZHANG Rong-Yue, HUANG Ying-Kun. Evaluation of natural resistance to smut in elite sugarcane varieties (lines) [J]. Acta Agronomica Sinica, 2021, 47(11): 2290-2296.
[11] ZHANG Hai, CHENG Guang-Yuan, YANG Zong-Tao, WANG Tong, LIU Shu-Xian, SHANG He-Yang, ZHAO He, XU Jing-Sheng. Cloning of sugarcane ScCRT1 gene and its response to SCMV infection [J]. Acta Agronomica Sinica, 2021, 47(1): 94-103.
[12] ZHENG Qing-Lei,YU Chen-Jing,YAO Kun-Cun,HUANG Ning,QUE You-Xiong,LING Hui,XU Li-Ping. Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC [J]. Acta Agronomica Sinica, 2020, 46(6): 844-857.
[13] LUO Jun,LIN Zhao-Li,LI Shi-Yan,QUE You-Xiong,ZHANG Cai-Fang,YANG Zai-Qi,YAO Kun-Cun,FENG Jing-Fang,CHEN Jian-Feng,ZHANG Hua. Effects of different soil improvement measures on soil physicochemical properties and microbial community structures in mechanically compacted acidified sugarcane field [J]. Acta Agronomica Sinica, 2020, 46(4): 596-613.
[14] WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[15] ZHANG Hai, LIU Shu-Xian, YANG Zong-Tao, WANG Tong, CHENG Guang-Yuan, SHANG He-Yang, XU Jing-Sheng. Sugarcane PsbS subunit response to Sugarcane mosaic virus infection and its interaction with 6K2 protein [J]. Acta Agronomica Sinica, 2020, 46(11): 1722-1733.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd