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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (10): 1503-1510.doi: 10.3724/SP.J.1006.2019.91011

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357

CHEN Fang1,QIAO Lin-Yi2,3,LI Rui2,LIU Cheng4,LI Xin2,3,GUO Hui-Juan2,ZHANG Shu-Wei2,CHANG Li-Fang2,LI Dong-Fang5,YAN Xiao-Tao2,REN Yong-Kang2,ZHANG Xiao-Jun2,3,*(),CHANG Zhi-Jian2,3,*()   

  1. 1College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China
    2Institute of Crop Science, Shanxi Academy of Agricultural Sciences / Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Taiyuan 030006, Shanxi, China
    3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture, Taiyuan 030006, Shanxi, China
    4Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China
    5Sixth Agricultural Division Agricultural Science Research Institute of Xinjiang Production and Construction Crops, Wujiaqu 831300, Xinjiang, China
  • Received:2019-01-18 Accepted:2019-05-12 Online:2019-10-12 Published:2019-09-10
  • Contact: Xiao-Jun ZHANG,Zhi-Jian CHANG E-mail:zxjemail@163.com;wrczj@126.com
  • Supported by:
    This study was supported by the National Key Research and Development Program of China(2017YFD0100600);Shanxi Academy of Agricultural Sciences(YGG17123);Shanxi Academy of Agricultural Sciences(YCX2018D2YS01);the National Natural Science Foundation of Shandong Province(ZR2017MC004);the Key Research and Development Program of Shanxi Province(201703D211007);the Key Research and Development Program of Shanxi Province(201803D221018-5);the Key Research and Development Program of Shanxi Province(201803D421020);the Key Scientific and Technological Innovation Platform(201605D151002)

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Abstract:

Powdery mildew is a serious disease affecting yield and quality of wheat. Wheat-Thinopyrum imtermedium introgression line CH1357 is highly resistant to Blumeria graminis f. sp. tritici (Bgt) at the adult plant stage and immune or highly resistant to 27 Bgt isolates at the seedling stage. Two mapping populations (F1, BC1, and F2:3) derived from Taichang 29/CH1357 and Mianyang 11/CH1357 were used to map the powdery mildew resistance gene by the bulked segregant analysis. The powdery mildew resistance in CH1357 was controlled by a single dominant gene, temporarily named PmCH1357. This gene was located on the short arm of chromosome 5D and linked to SSR markers Xcfd81 and Xbwm8, with genetic distances of 2.0 cM and 11.3 cM in the Taichang 29/CH1357 population and 1.5 cM and 8.9 cM in the Mianyang 11/CH1357 population, respectively. PmCH1357 differs from other Pm genes reported on chromosome 5DS in resistance spectrum, which may be a new source of resistance.

Key words: resistance to powdery mildew, SSR markers, linkage map, gene mapping, Pm2

Table 1

Seedling infection types of CH1357, Taichang 29 (TC29), and Mianyang 11 (MY11) to 32 Bgt isolates"

白粉菌株Bgt 材料Material 白粉菌株Bgt 材料Material 白粉菌株Bgt 材料Material
CH1357 TC29 MY11 CH1357 TC29 MY11 CH1357 TC29 MY11
B09 0 4 4 B33 0 4 4 B50 3 4 4
B13 0 4 4 B37 0 4 4 B51 2 4 4
B14 4 4 4 B38 1 4 4 B52 0 4 3
B15 0 4 4 B39 0 4 4 B55 0 4 4
B17 0 4 4 B40 0 4 4 B56 0 4 4
B21 0 4 4 B41 0 4 4 B57 0 4 4
B22 0 4 4 B42 0 4 4 E09 0 4 4
B23 0 4 3 B44 0 4 4 E18 4 4 4
B25 0 4 4 B46 0 4 3 E21 0 4 4
B28 0 4 4 B48 0 4 4 E23-1 0 4 4
B30 0 4 4 B49 0 4 4 - - - -

Table 2

Powdery mildew reactions and segregation ratios in different generations from cross of Taichang 29/CH1357 and Mianyang 11/CH1357 following inoculation with Bgt isolate E09 at the seedling stage"

亲本/组合
Parent/cross		 对白粉菌株E09的侵染类型 Infection types to Bgt isolates E09 理论比率
Theoretical ratio
R:S		 χ2值
χ2 value		 P值
P-value
0 0; 1 2 3 4
CH1357 (P1) 12 8
台长29 Taichang 29 (P2) 5 15
绵阳11 Mianyang 11 (P3) 5 14
P2/P1 (F1) 13 7
P1/P2 (F1) 15 5
P2/P1//P2 (BC1) 14 10 4 2 12 17 1:1 0.017 0.896
P3/P1 (F1) 14 5
P1/P3 (F1) 16 4
P3/P1//P3 (BC1) 12 14 2 1 12 15 1:1 0.071 0.789

Table 3

Resistant pathotype to Bgt E09 of two F2:3 populations"

F2: 3家系表现型
Phenotype of F2:3 lines		 杂交组合 Hybrid cross
CH1357/Taichang 29 CH1357/Mianyang 11
实际值 Actual value 期望值 Expected value 实际值 Actual value 期望值 Expected value
纯合抗病株 Resistant plants 120 120.75 108 102.75
抗性分离株 Heterozygous plants 231 241.50 208 205.50
纯合感病株 Susceptible plants 132 120.75 95 102.75
合计 Total 483 411
χ2 (1:2:1) 1.509 0.883
P值P-value 0.470 0.643

Fig. 1

Amplification result of SSR markers Xcfd81 and Xgwm190 in F2 population from wheat variety CH1357 M: marker; Pr: resistant parent CH1357; Ps: susceptible parent Taichang 29; Br: resistant bulk; Bs: susceptible bulk; 1-6: homozygous resistant plants; 7-12: homozygous susceptible plants."

Fig. 2

Genetic mapping of PmCH1357 with populations Taichang 29/CH1357 (a) and Mianyang 11/CH1357 (b) and comparison with other genes in Pm2 loci"

Fig. 3

Comparison of linkage markers in CH1357 with some documented stocks with known powdery mildew resistance genes on chromosome arm 5DS M: marker; 1: Taichang 29; 2:CH1357; 3: UIka (Pm2a); 4: KM2939 (Pm2b); 5: Niaomai (Pm2c)."

Table 4

Comparison of responses of CH1357 and lines with known powdery mildew resistance genes on chromosome arm 5DS to 12 Bgt pathotypes"

品种/品系
Variety/line		 抗白粉病基因
Pm gene		 白粉菌株 Blumeria graminis tritici isolate
B13 B14 B38 B40 B41 B50 B51 B56 E09 E18 E21 E23-1
CH1357 PmCH1357 0 4 1 0 0 3 2 0 0 4 0 0
UIka/8*Cc Pm2a 0 4 3 3 1 4 3 0; 0; 4 0; 0;
KM2939 Pm2b 0 4 1 0 0; 4 0 0; 0; 1 3 0;
鸟麦Niaomai Pm2c 0 0 2 0 0 4 0 0 0; 0 0; 0;
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