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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (02): 157-165.doi: 10.3724/SP.J.1006.2020.91048

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Mapping and genetic analysis of QTLs for Fusarium head blight resistance to disease spread in Yangmai 16

HU Wen-Jing1,ZHANG Yong1,LU Cheng-Bin1,WANG Feng-Ju2,LIU Jin-Dong2,JIANG Zheng-Ning1,WANG Jin-Ping2,ZHU Zhan-Wang2,XU Xiao-Ting2,HAO Yuan-Feng2,HE Zhong-Hu2,3,GAO De-Rong1,*()   

  1. 1 Lixiahe Institute of Agriculture Sciences / Key Laboratory of Wheat Biology and Genetic Improvement for Low & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs, Yangzhou 225007, Jiangsu, China
    2 National Wheat Improvement Center / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3 CIMMYT-China Office, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-07-22 Accepted:2019-09-26 Online:2020-02-12 Published:2019-10-09
  • Contact: De-Rong GAO E-mail:gdr@wheat.org.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31901544);China Agriculture Research System(CARS-03-03B);China Agriculture Research System(CARS-3-2-11);the National Key Research and Development Program of China(2017YFD0100801);the National Key Research and Development Program of China(2017YFD0101802);the Natural Science Foundation of Jiangsu Province(BK20171279)

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Abstract:

Fusarium head blight (FHB) resistance of Yangmai wheat cultivars has been paid much attention, but the underlying genetic mechanism is unclear. In recent years, Yangmai 16 is a predominant wheat cultivar durably resistant to FHB in production. A population of 174 double haploid lines (DH) produced by crossing Yangmai 16 (YM16) with the susceptible cultivar Zhongmai 895 (ZM895) was evaluated for FHB response using point inoculation from 2017 to 2019. The DH population was genotyped with wheat 660K SNP array and a high-density genetic map was constructed. Six resistance QTLs were detected, and among them, five were from the resistant parent Yangmai 16 and one from Zhongmai 895. QFhb.yaas-4DS and QFhb.yaas-6AS were detected at least in two years, explaining 8.8% to 15.0% of the phenotypic variances, respectively. QFhb.yaas-2DL and QFhb.yaas-3BL were detected only in one year, accounting for 10.5% and 14.7% of the phenotypic variances. QFhb.yaas-5BL and QFhb.yaas-4BS were detected in one year, too, accounting for 6.4% and 8.3% of the phenotypic variances, respectively. Pyramiding of multiple resistant loci with large effects (>10%) is an effective approach to increase FHB resistance. The QTLs identified from Yangmai 16 in the present study will provide a starting point for genetic studies of other Yangmai cultivars, and the QTLs closely linked to markers will be useful for marker-assisted selection in wheat FHB improvement.

Key words: Triticum aestivum, Fusarium head blight, QTL, marker-assisted breeding

Table 1

Statistic analysis of FHB severity for the Yangmai 16/Zhongmai 895 DH lines, their parents, and checks"

年份
Year		 亲本 Parents (%) 对照 CK (%) DH群体 DH population
扬麦16
Yangmai 16		 中麦895
Zhongmai 895		 苏麦3
Sumai 3		 周麦18
Zhoumai 18		 平均值
Mean (%)		 标准差
SD		 最大值
Max. (%)		 最小值
Min. (%)		 峰度
Kurt.		 偏度
Skew.		 遗传力
Hereditability
2017 14.2 A 58.7 B 5.2 A 60.2 B 29.1 18.9 83.7 3.4 0.2 0.9 0.67
2018 17.9 A 65.0 B 5.6 A 62.3 B 53.5 19.9 100.0 6.6 -0.7 -0.2 0.74
2019 23.3 A 69.7 B 6.1 A 70.1 B 49.5 18.1 89.8 10.3 -0.5 0.1 0.82

Fig. 1

Frequency distributions of DH lines with different FHB severity (2017-2019) The abscissa indicates the percentage of scabbed spikelets, and the ordinate indicates the line number."

Table 2

QTL for FHB resistance mapped in the Yangmai 16/Zhongmai 895 DH population"

QTL 年份
Year		 物理位置
Physical position (Mb)		 遗传位置
Genetic position (cM)		 标记区间
Marker interval		 LOD 贡献率
PVE (%)		 加性效应#
Add#
QFhb.yaas-2DL 2017 512.7-532.0 49.6 AX_111765614-AX_109738482 4.7 10.5 -6.1
QFhb.yaas-3BL 2019 637.1-647.2 138.6 AX_94528202-AX_109001202 8.9 14.7 -6.9
QFhb.yaas-4DS 2017 27.1-33.1 61.9 AX_109962849-AX_111071805 5.6 13.7 -7.2
2018 18.3-19.3 54.7 AX_111311200-AX_89421921 4.4 8.8 -5.9
2019 15.9-16.6 53.7 AX_94558069-AX_95150762 6.2 9.5 -5.6
QFhb.yaas-5BL 2018 587.1-588.8 138.7 AX_94646656-AX_109853998 3.2 6.4 -5.0
QFhb.yaas-6AS 2018 12.0-12.4 64.9 AX_109294414-AX_108848013 4.6 9.5 -6.2
2019 12.0-12.4 65.0 AX_109294414-AX_108848013 8.9 15.0 -7.1
QFhb.yaas-4BS 2018 82.7-91.3 14.7 AX_110472183-AX_94859572 4.1 8.3 5.8

Fig. 2

Mapped QTL for FHB resistance in the Yangmai 16/Zhongmai 895 DH population Markers’ names are shown to the right of vertical axis, and their genetic positions are shown in cM to the left. Red, blue, and green represent 2017, 2018, and 2019, respectively. LOD values of QTL are shown on the right side."

Table 3

FHB severity of the Yangmai 16/Zhongmai 895 DH lines with different QTL-combinations"

QTL组成
QTL combination		 家系数
Number of lines		 最小值
Minimum		 最大值
Maximum		 平均
Mean		 标准差
Standard deviation		 方差
Variance
None 46 26.1 88.0 54.3 A 13.7 187.6
QFhb.yaas-4DS 62 18.6 71.3 43.2 B 13.1 172.0
QFhb.yaas-6AS 28 18.9 78.8 44.8 B 16.4 269.6
QFhb.yaas-4DS+QFhb.yaas-6AS 38 9.9 57.4 32.3 C 11.2 126.5

Table 4

FHB severity of the Yangmai 16/Zhongmai 895 DH lines with different QTL-combinations"

QTL数
Number of QTL		 家系数
Number of lines		 平均PSS (100%)#
Average of PSS (100%)#		 不同PSS范围内的家系数目 Number of lines in different PSS range
0-25 25-50 50-75 75-100
0 7 67.1 A 0 0 6 1
1 46 53.8 B 1 15 28 2
2 65 41.8 C 8 38 19 0
3 46 36.9 CD 10 29 7 0
4 10 30.5 D 2 8 0 0

Fig. 3

Relationship between DH lines with QTLs and FHB severity across environments PSS (percentage of scabbed spikelets) indicates the FHB severity."

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