一种绿豆柱头外露突变体的转录组分析

doi:10.3724/SP.J.1006.2024.34080

Abstract

Abstract:

Stigma exsertion has been widely used in hybrid breeding as an excellent trait to improve crop outcrossing rate, seed purity, and seed production cost. As a closed-pollinated crop, few stigma exsertion mutants have been reported in mungbean. A stigma exsertion mutant se2 was discovered in mungbean variety Jilyu 7 after chemical mutagination. In order to clarify the molecular mechanism of stigma exsertion, transcription-sequencing (RNA-seq) analysis was conducted on the next day's opening buds of se2 and its wild type Jilyu 7. A total of 572 differentially expressed genes (DEGs) were obtained in se2, among which 262 DEGs were up-regulated and 310 DEGs were down-regulated, based on the screening criteria of difference multiplier |log₂(Fold Change)| ≥1 and P ≤ 0.05. In GO database, differentially expressed genes were significantly enriched in biological processes such as metabolism and biosynthesis, and localized in regions such as apoplast, cell walls, and membranes, and mainly associated with molecular functions such as binding and redox. In the kyoto encyclopedia of genes and genome (KEGG) database, differentially expressed genes were significantly enriched in plant hormone signal transduction and biosynthesis of secondary metabolites. Functional annotation revealed many genes related to cell wall synthesis and metabolism, cell division and cell expansion, and plant hormones. Therefore, we hypothesized that cell division, cell expansion, and plant hormone signaling processes of the keel flap in se2 mutants were affected, leading to stigma exsertion. This study laid a foundation for future investigations into the molecular mechanism of stigma exsertion in mungbean and its application in heterosis.

Key words: mungbean, stigma exsertion, mutant, transcriptome, high-throughput sequencing, heterosis

SONG Meng-Yuan, GUO Zhong-Xiao, SU Yu-Fei, DENG Kun-Peng, LAN Tian-Jiao, CHENG Yu-Xin, BAO Shu-Ying, WANG Gui-Fang, DOU Jin-Guang, JIANG Ze-Kai, WANG Ming-Hai, XU Ning. Transcriptome analysis of a stigma exsertion mutant in mungbean[J].Acta Agronomica Sinica, 2024, 50(4): 957-968.

Figures/Tables 10

Fig. 1

Table 1

Table 2

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Table 3

Differentially expressed genes associated with cell division and cell expansion"

基因编号 Gene ID	上调/下调 Up/down	基因名称 Gene name	描述 Description
jg13552	Down	Laccase-15	漆酶15 Laccase-15
jg12507	Down	Laccase-2	漆酶2 Laccase-2
jg17193	Down	Laccase-4	漆酶4 Laccase-4
jg15432	Down	callose synthase 8	推测的胼胝质合酶8 Putative callose synthase 8
jg25641	Down	PE6/PME6	可能的果胶甲酯酶6/抑制剂6 Probable pectinesterase/pectinesterase inhibitor 6
jg33131	Up	PMEI 18/PME4	果胶甲酯酶/果胶甲酯酶抑制剂18 Pectinesterase/pectinesterase inhibitor 18
jg11460	Down	NAO	乙酰鸟氨酸脱酰基酶 Acetylornithine deacetylase
jg13187	Up	CEL1	纤维素酶1 Cellulase 1
jg21473	Down	XTH-7	木葡聚糖内转糖苷酶/水解酶7 XTH-7
jg24723	Up	XTH-16	木葡聚糖内转糖苷酶/水解酶16 XTH-16
jg20410	Down	XTH-22		木葡聚糖内转糖苷酶/水解酶22 XTH-22
jg20409	Down	XTH-22		木葡聚糖内转糖苷酶/水解酶22 XTH-22
jg20407	Down	XTH-23		木葡聚糖内转糖苷酶/水解酶23 XTH-23
jg20408	Down	XTH-23		木葡聚糖内转糖苷酶/水解酶23 XTH-23
jg12255	Up	XTH-31		木葡聚糖内转糖苷酶/水解酶31 XTH-31
jg11149	Down	GDP-fucose protein O-fucosyltransferase		GDP-fucose O-岩藻糖基转移酶 GDP-fucose protein O-fucosyltransferase
jg9115	Up	MYB46		转录因子MYB46 Transcription factor MYB46
jg17738	Down	GDPDL4		甘油磷酸二酯磷酸二酯酶GDPDL4 Glycerophosphodiester phosphodiesterase-like 4
jg23957	Down	1,3-beta-glucanase 7		1,3-β-葡聚糖内水解酶7 1,3-beta-glucan endohydrolase 7
jg9059	Up	Beta-glucosidase 12		β-葡萄糖苷酶12 Beta-glucosidase 12
jg16663	Up	Beta-1,3-endoglucanase 8		β-1.3内切葡聚糖酶8 Beta-1,3-endoglucanase 8
jg23043	Down	RAY1		β-阿拉伯呋喃糖基转移酶 Beta-arabinofuranosyltransferase RAY1
jg36915	Down	Cell wall beta-fructosidase 1		细胞壁β-果糖苷酶1 Cell wall beta-fructosidase 1
jg21315	Up	Probable aspartyl protease		可能的天冬氨酰蛋白酶At4g16563 Probable aspartyl protease At4g16563
jg17593	Down	Aspartyl protease family protein 2		天冬氨酰蛋白酶家族蛋白2 Aspartyl protease family protein 2
jg39446	Down	LRX/Extensin 6		富含亮氨酸重复延伸蛋白样蛋白6 Leucine-rich repeat extensin-like protein 6
jg15038	Down	IRX14H		β-1,4-木糖基转移酶IRX14H Probable beta-1,4-xylosyltransferase IRX14H
jg12968	Down	EXPLA2		扩展蛋白EXPLA2 Expansin-like A2
jg19405	Down	EXORDIUM		蛋白EXORDIUM Protein EXORDIUM
jg19402	Down	EXORDIUM		蛋白EXORDIUM Protein EXORDIUM
jg19404	Down	EXORDIUM		蛋白EXORDIUM Protein EXORDIUM
jg20020	Down	COBRA-Like protein 7		COBRA样蛋白7 COBRA-like protein 7
jg29779	Down	Sec3b		囊泡转运复合体亚基Sec3B Exocyst complex component SEC3B
jg15257	Down	KIN-6		驱动蛋白KIN-6 Kinesin-like protein KIN-6
jg15248	Down	KIN-6		驱动蛋白KIN-6 Kinesin-like protein KIN-6
jg35033	Down	KIN-7M		驱动蛋白KIN-7M Kinesin-like protein KIN-7M
jg14893	Up	KIN-5C		驱动蛋白KIN-5C Kinesin-like protein KIN-5C
jg33119	Down	DUF1005		未知功能蛋白DUF1005 Protein of unknown function DUF1005
jg33683	Up	BTB/POZ domain		BTB/POZ结构域蛋白 BTB/POZ domain-containing protein
jg15199	Down	PICKLE/CHR5		CHD3型染色质重塑因子PICKLE CHD3-type chromatin-remodeling factor PICKLE
jg15252	Down	PICKLE/CHR6		CHD3型染色质重塑因子PICKLE CHD3-type chromatin-remodeling factor PICKLE
jg2927	Up	EBP1		ERBB3结合蛋白1 ERBB-3 BINDING PROTEIN 1
jg13226	Down	CCR4		丝氨酸/苏氨酸蛋白激酶样蛋白CCR4 Serine/threonine-protein kinase-like protein CCR4
jg15053	Down	PAS1		肽基脯氨酰顺反异构酶 Peptidyl-prolyl cis-trans isomerase PASTICCINO1
jg15144	Up	SLAH3		S型阴离子通道3 S-type anion channel SLAH3
jg19729	Down	SLAH1		S型阴离子通道1 S-type anion channel SLAH1
jg25789	Down	SKOR		外向整流K+通道SKOR Stelar K(+) outward rectifying channel
jg17957	Down	CLC-e		氯离子通道蛋CLC-e Chloride channel protein CLC-e

Table 3

Table 4

Differentially expressed genes associated with plant hormones"

基因编号 Gene ID	上调/下调 Up/down	基因名称 Gene name	描述 Description
jg16675	Down	AUX1-like protein 1	生长素输入载体AUX1 Auxin transporter-like protein 1
jg2587	Down	PIN-Likes 1	生长素输出蛋白PIN-Likes 1 Protein PIN-Likes 1
jg20452	Up	VAN3-binding	VAN3结合蛋白 VAN3-binding protein
jg12839	Up	ARG7	生长素相应蛋白ARG7 Auxin responsive protein
jg28855	Up	SAUR71	生长素响应蛋白SAUR71 Auxin-responsive protein SAUR71
jg13096	Down	CKX6/CKO6	细胞分裂素脱氢酶/氧化酶6 Cytokinin dehydrogenase/oxidase 6
jg33851	Up	ATHB-40	HD-ZIP蛋白ATHB-40 HD-ZIP protein ATHB-40
jg638	Up	ATHB-12	HD-ZIP蛋白 ATHB-12 HD-ZIP protein ATHB-12
jg1095	Up	GA 20-oxidase 2	赤霉素20氧化酶2 Gibberellin 20 oxidase 2
jg18873	Up	GA 20-oxidase 2	赤霉素20氧化酶2 Gibberellin 20 oxidase 2
jg36069	Up	ETR2	乙烯受体ETR2 Ethylene receptor 2
jg36578	Up	ERF106	乙烯响应转录因子ERF106 Ethylene-responsive transcription factor ERF106
jg4916	Up	ERF034	乙烯响应转录因子ERF034 Ethylene-responsive transcription factor ERF034
jg37484	Up	ERF1B	乙烯响应转录因子ERF1B Ethylene-responsive transcription factor 1B
jg38226	Down	PP2C	可能的蛋白磷脂酶 PP2C 49 Probable protein phosphatase 2C 49
jg545	Up	MARD1/SAG102	ABA调节的休眠介质1/衰老相关蛋白SAG102 Mediator of ABA-regulated dormancy1/Senescence-associated protein SAG102
jg20855	Up	AFP3	ABI5结合蛋白3 ABI5-binding protein 3
jg27356	Up	ABR1	乙烯响应因子ABR1 Ethylene-responsive transcription factor ABR1
jg29371	Up	AOS2/P450 74A	丙二烯氧化物合酶2 Allene oxide synthase 2/Cytochrome P450 74A
jg35914	Down	TGA7	转录因子TGA7 Transcription factor TGA7
jg15164	Down	OPP21	双组分响应调节子ORR21 Two-component response regulator ORR21
jg25674	Down	ARR14	双组分响应调节子ARR14 Two-component response regulator ARR14
jg27911	Up	ARR17	双组分响应调节子ARR17 Two-component response regulator ARR17
jg14455	Down	SAP12	锌指结构域应激相关蛋白12 Zinc finger AN1 domain-containing stress-associated protein 12 SAP12
jg14454	Down	SAP12	锌指结构域应激相关蛋白12 Zinc finger AN1 domain-containing stress-associated protein 12 SAP12

Table 4

References 25

[26]	胡育玮. 烟草细胞质遗传柱头外露发生机制研究. 河南农业大学硕士学位论文, 河南郑州, 2019.
	Hu Y W. Study on the Stigma Exsertion Formation Mechanism in Cytoplasmic Inheritance of Stigma Exsertion Tobacco. MS Thesis of Henan Agricultural University, Zhengzhou, Henan, China, 2018. (in Chinese with English abstract)
[27]	Li J X, Li M, Wang W M, Wang D, Hu Y W, Zhang Y Y, Zhang X Q. Morphological and physiological mechanism of cytoplasmic inheritance stigma exsertion trait expression in tobacco (Nicotiana tabacu). Plant Sci, 2023, 326: 111528. doi: 10.1016/j.plantsci.2022.111528
[28]	Smyczynski C, Roudier F, Gissot L, Vaillant E, Grandjean O, Morin H, Masson T, Bellec Y, Geelen D, Faure J D. The C terminus of the immunophilin pasticcino1 is required for plant development and for interaction with a NAC-like transcription factor. J Biol Chem, 2006, 281: 25475-25484. doi: 10.1074/jbc.M601815200 pmid: 16803883
[29]	Garcia M A, Koonrugsa N, Toda T. Two kinesin-like KIN I family proteins in fission yeast regulate the establishment of metaphase and the onset of anaphase A. Curr Biol, 2002, 12: 610-621. doi: 10.1016/s0960-9822(02)00761-3 pmid: 11967147
[30]	Becraft P W, Stinard P S, McCarty D R. Crinkly4: a tnfr-like receptor kinase involved in maize epidermal differentiation. Science, 1996, 273: 1406-1409. doi: 10.1126/science.273.5280.1406 pmid: 8703079
[31]	Kang S G, Lee H J, Suh S G. The maize Crinkly4 gene is expressed spatially in vegetative and floral organs. Plant Biol, 2002, 45: 219-224.
[32]	Pu C X, Ma Y, Wang J, Zhang Y C, Jiao X W, Hu Y H, Wang L L, Zhu Z G, Sun D, Sun Y. Crinkly4 receptor-like kinase is required to maintain the interlocking of the palea and lemma, and fertility in rice, by promoting epidermal cell differentiation. Plant J, 2012, 70: 940-953. doi: 10.1111/tpj.2012.70.issue-6
[33]	Cosgrove D J. Growth of the plant cell wall. Nat Rev Mol Cell Biol, 2005, 6: 850-861.
[34]	Chen K Y, Cong B, Wing R, Vrebalov J, Tanksley S D. Changes in regulation of a transcription factor lead to autogamy in cultivated tomatoes. Science, 2007, 318: 643-645. doi: 10.1126/science.1148428
[35]	Cheng M, Gong C, Zhang B, Qu W, Qi H, Chen X, Wang X, Zhang Y, Liu J, Ding X, Qiu Y, Wang A. Morphological and anatomical characteristics of exserted stigma sterility and the location and function of SLLST (Solanum lycopersicum long styles) gene in tomato. Theor Appl Genet, 2021, 134: 505-518. doi: 10.1007/s00122-020-03710-0 pmid: 33140169
[36]	Guo N, Wang Y, Chen W, Tang S, An R, Wei X, Hu S, Tang S, Shao G, Jiao G, Xie L, Wang L, Sheng Z, Hu P. Fine mapping and target gene identification of QSE4, a QTL for stigma exsertion rate in rice (Oryza sativa L.). Front Plant Sci, 2022, 13: 959859. doi: 10.3389/fpls.2022.959859
[37]	Zhao M, Han Y, Feng Y, Li F, Wang W. Expansins are involved in cell growth mediated by abscisic acid and indole-3-acetic acid under drought stress in wheat. Plant Cell Rep, 2012, 31: 671-685. doi: 10.1007/s00299-011-1185-9
[38]	Pan C, Yang D, Zhao X, Jiao C, Yan Y, Lamin-Samu A T, Wang Q, Xu X, Fei Z, Lu G. Tomato stigma exsertion induced by high temperature is associated with the jasmonate signalling pathway. Plant Cell Environ, 2019, 42: 1205-1221. doi: 10.1111/pce.v42.4
[39]	Cheng H, Qin L, Lee S, Fu X, Richards D E, Cao D, Luo D, Harberd N P, Peng J. Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function. Development, 2004, 131: 1055-1064. doi: 10.1242/dev.00992 pmid: 14973286
[40]	王燕, 潘长田, 王洁, 秦力, 邹滔, 卢钢. 赤霉素对亚高温胁迫下番茄花柱外露及相关基因表达的影响. 浙江大学学报(农业与生命科学版), 2015, 41: 449-457.
	Wang Y, Pan C T, Wang J, Qin L, Zou T, Lu G. Effects of gibberellin on tomato stigma exsertion and hormone-related gene expression under moderate heat stress. J Zhejiang Univ (Agric Life Sci Edn), 2015, 41: 449-457. (in Chinese with English abstract)
[41]	Carrera E, Ruiz-Rivero O, Peres L E P, Atares A, Garcia-Martinez J L. Characterization of the procera tomato mutant shows novel functions of the sldella protein in the control of flower morphology, cell division and expansion, and the auxin-signaling pathway during fruit-set and development. Plant Physiol, 2012, 160: 1581-1596. doi: 10.1104/pp.112.204552 pmid: 22942390
[42]	Rieu I, Ruiz R O, Fernandez G N, Griffiths J, Powers S J, Gong F, Linhartova T, Eriksson S, Nilsson O, Thomas S G, Phillips A L, Hedden P. The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle. Plant J, 2008, 53: 488-504. doi: 10.1111/j.1365-313X.2007.03356.x pmid: 18069939
[43]	Brioudes F, Joly C, Szécsi J, Varaud E, Leroux J, Bellvert F, Bertrand C, Bendahmane M. Jasmonate controls late development stages of petal growth in Arabidopsis thaliana. Plant J, 2009, 60: 1070-1080. doi: 10.1111/tpj.2009.60.issue-6
[44]	Dong S, Tarkowska D, Sedaghatmehr M, Welsch M, Gupta S, Mueller-Roeber B, Balazadeh S. The HB40-JUB1 transcriptional regulatory network controls gibberellin homeostasis in Arabidopsis. Mol Plant, 2022, 15: 322-339.
[45]	Son O, Hur Y S, Kim Y K, Lee H J, Kim S, Kim M R, Nam K H, Lee M S, Kim B Y, Park J, Park J, Lee S C, Hanada A, Yamaguchi S, Lee I J, Kim S K, Yun D J, Söderman E, Cheon C I. ATHB12, an ABA-inducible homeodomain-leucine zipper (HD- Zip) protein of Arabidopsis, negatively regulates the growth of the inflorescence stem by decreasing the expression of a gibberellin 20-oxidase gene. Plant Cell Physiol, 2010, 51: 1537-1547. doi: 10.1093/pcp/pcq108
[1]	郑卓杰. 中国食用豆类学. 北京: 中国农业出版社, 1997. pp 141-166.
	Zheng Z J. Food Legumes in China. Beijing: China Agriculture Press, 1995. pp 141-166. (in Chinese)
[2]	田静, 程须珍, 范保杰, 王丽侠, 刘建军, 刘长友, 王素华, 曹志敏, 陈红霖, 王彦, 王珅. 我国绿豆品种现状及发展趋势. 作物杂志, 2021, (6): 15-21.
	Tian J, Cheng X J, Fan B J, Wang L X, Liu J J, Liu C Y, Wang S H, Cao Z M, Chen H L, Wang Y, Wang K. Current situation and development trend of mungbean varieties in China. Crops, 2021, (6): 15-21. (in Chinese with English abstract)
[3]	王丽侠, 程须珍, 王素华. 绿豆种质资源､育种及遗传研究进展. 中国农业科学, 2009, 42: 1519-1527.
	Wang L X, Cheng X Z, Wang S H. Advances in research on genetic resources, breeding and genetics of mungbean (Vigna radiata L.). Sci Agric Sin, 2009, 42: 1519-1527. (in Chinese with English abstract)
[4]	黄穗兰, 郭宝德, 冀丽霞, 牛永章, 姜艳丽. 棉花种间杂交长柱头种质系TY35的培育与应用. 山西农业科学, 2015, 43: 777-779.
	Huang S L, Guo B D, Ji L X, Niu Y Z, Jiang Y L. The selection and application of germplasm line TY35 with long stigma from interspecific crossing in cotton. J Shanxi Agric Sci, 2015, 43: 777-779. (in Chinese with English abstract)
[5]	杨保汉. 不育系柱头外露率及其结实率研究. 杂交水稻, 1997, (1): 15-17.
	Yang B H. Studies on stigma exsertion rate and outcrossing rate of CMS Lines in rice. Hybrid Rice, 1997, (1): 15-17. (in Chinese)
[6]	崔贵梅, 牛天堂, 张福耀, 袁爱萍, 孙毅. 谷子(Setaria italica Beauv.)高异交结实雄性不育系“81-16”的柱头性状观察. 作物学报, 2007, 33: 149-153.
	Cui G M, Niu T T, Zhang F Y, Yuan A P, Sun Y. The stigma observation on foxtail millet (Setaria italica Beauv.) male-sterile line “81-16” with high outcross seed setting. Acta Agron Sin, 2007, 33: 149-153. (in Chinese with English abstract)
[7]	Lin Y, Laosatit K, Chen J, Yuan X, Wu R, Amkul K, Chen X, Somta P. Mapping and functional characterization of stigma exposed 1, a DUF1005 gene controlling petal and stigma cells in mungbean (Vigna radiata). Front Plant Sci, 2020, 11: 575922. doi: 10.3389/fpls.2020.575922
[8]	Yan H, Zhang B, Zhang Y, Chen X, Xiong H, Matsui T, Tian X. High temperature induced glume closure resulted in lower fertility in hybrid rice seed production. Front Plant Sci, 2017, 7: 1960.
[9]	Elshamey E A Z, Hamad H S, Alshallash K S, Alghuthaymi M A, Ghazy M I, Sakran R M, Selim M E, ElSayed M A A, Abdelmegeed T M, Okasha S A, Behiry S I, Boudiar R, Mansour E. Growth regulators improve outcrossing rate of diverse rice cytoplasmic male sterile lines through affecting floral traits. Plants (Basel), 2022, 11: 1291. doi: 10.3390/plants11101291
[10]	Matthias Benoit. From non-self to self: stepwise mutations in transcription factors promote the transition to self-pollination in tomato. Plant Cell, 2021, 10: 3183-3184.
[11]	Riccini A, Picarella M E, De Angelis F, Mazzucato A. Bulk RNA-Seq analysis to dissect the regulation of stigma position in tomato. Plant Mol Biol, 2021, 105: 263-285. doi: 10.1007/s11103-020-01086-9 pmid: 33104942
[12]	Pan C, Ye L, Zheng Y, Wang Y, Yang D, Liu X, Chen L, Zhang Y, Fei Z, Lu G. Identification and expression profiling of micrornas involved in the stigma exsertion under high-temperature stress in tomato. BMC Genomics, 2017, 18: 843. doi: 10.1186/s12864-017-4238-9 pmid: 29096602
[13]	Mortazavi A, Williams B A, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods, 2008, 5: 621-628. doi: 10.1038/nmeth.1226 pmid: 18516045
[14]	Trapnell C, Williams B A, Pertea G, Mortazavi A, Kwan G, van Baren M J, Salzberg S L, Wold B J, Pachter L. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol, 2010, 28: 511-515. doi: 10.1038/nbt.1621 pmid: 20436464
[15]	Love M I, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-Seq data with DESeq2. Genome Biol, 2014, 15: 550. doi: 10.1186/s13059-014-0550-8
[16]	Bashline L, Lei L, Li S, Gu Y. Cell wall, cytoskeleton, and cell expansion in higher plants. Mol Plant, 2014, 7: 586-600. doi: 10.1093/mp/ssu018 pmid: 24557922
[17]	Tsabary G, Shani Z, Roiz L, Levy I, Riov J, Shoseyov O. Abnormal ‘wrinkled’ cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-β-glucanase (cell) antisense. Plant Mol Biol, 2003, 51: 213-224. pmid: 12602880
[18]	Herger A, Dünser K, Kleine-Vehn J, Ringli C. Leucine-rich repeat extensin proteins and their role in cell wall sensing. Curr Biol, 2019, 29: 851-858. doi: S0960-9822(19)30884-X pmid: 31505187
[19]	Fendrych M, Synek L, Pečenková T, Toupalová H, Cole R, Drdová E, Nebesářová J, Šedinová M, Hála M, Fowler J E, Žárský V. The Arabidopsis exocyst complex is involved in cytokinesis and cell plate maturation. Plant Cell, 2010, 22: 3053-3065. doi: 10.1105/tpc.110.074351
[20]	Wen T J, Hochholdinger F, Sauer M, Bruce W, Schnable P S. The roothairless1 gene of maize encodes a homolog of sec3, which is involved in polar exocytosis. Plant Physiol, 2005, 138: 1637-1643. doi: 10.1104/pp.105.062174
[21]	To J P C, Haberer G, Ferreira F J, Deruère J, Mason M G, Schaller G E, Alonso J M, Ecker J R, Kieber J J. Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. Plant Cell, 2004, 16: 658-671. doi: 10.1105/tpc.018978
[22]	吴健, 孙玥, 张融雪, 李军玲, 王晓静, 闫双勇, 马忠友, 孙林静, 苏京平, 王胜军, 刘学军. 水稻柱头外露率相关性状的调查及高柱头外露率不育系的创制. 天津农业科学, 2017, 23(11): 55-60.
	Wu J, Sun Y, Zhang R X, Li J L, Wang X J, Yan S Y, Ma Z Y, Sun L J, Su J P, Wang S J, Liu X J. Investigation of characters related to stigma exserted rate in rice and establishment of male sterile line with high stigma exposure. Tianjin Agric Sci, 2017, 23(11): 55-60. (in Chinese with English abstract)
[23]	Shang L, Song J, Yu H, Wang X, Yu C, Wang Y, Li F, Lu Y, Wang T, Ou-Yang B, Zhang J, Larkin R M, Ye Z, Zhang Y. A mutation in a C2H2-type zinc finger transcription factor contributed to the transition toward self-pollination in cultivated tomato. Plant Cell, 2021, 33: 3293-3308. doi: 10.1093/plcell/koab201
[24]	张栩佳, 胡灵芝, 陈哲皓, 李颖, 王利琳. 花器官大小调控机制的研究进展. 植物生理学报, 2014, 50: 691-697.
	Zhang X J, Hu L Z, Chen Z H, Li Y, Wang L L. Research progress in regulation mechanism of floral organ size. Plant Physiol J, 2014, 50: 691-697. (in Chinese with English abstract)
[25]	张鋆鋆. 烟草细胞质遗传柱头外露性状发育特征研究. 河南农业大学硕士学位论文, 河南郑州, 2018.
	Zhang Y Y. Study on the Developmental Characteristic of Cytoplasmic Inheritance of Tobacco Stigma Exsertion. MS Thesis of Henan Agricultural University, Zhengzhou, Henan, China, 2018. (in Chinese with English abstract)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因编号 Gene ID	正向引物序列 Forward sequence (5'-3')	反向引物序列 Reverse sequence (5'-3')
jg12155	CTTGGATACCATACCTGCGGACATG	CACGAACATCGGCAATTCTGAATGG
jg15370	TGTGGAGCAAGGAGCATCAACAAC	CTTAGCCAAATCCATGTCCCTTCCC
jg17957	CCCGGTGTTGCAACTTCTCT	CATAAGCTTGCGGTGATGCC
novel.24	TGTGTGGTGTCGCAGGTTGTTC	AGCAGTGAGGAGTGGTCCAAGAG
jg25789	CACCTCCCTCTACTTTGCCG	AGCGCCGAGAATCATGTCAA
VrActin	GGCATCCACGAGACAACA	AGCCTCCAATCCAGACAC

样本 Sample	测序总读数 Total reads number	质控后读数 Clean reads number	错误率 Error rate (%)	总比对率 Mapped ratio (%)	Q20碱基百分比 Q20 base percentage (%)	Q30碱基百分比 Q30 base percentage (%)
WT1	44719486	43961678	0.02	96.79	98.10	94.43
WT2	46637862	45775686	0.02	96.86	98.05	94.23
WT3	43888030	42753792	0.03	96.68	97.97	94.08
SE2-1	47065416	46312622	0.02	96.97	98.10	94.33
SE2-2	47644132	46668574	0.02	96.67	98.20	94.61
SE2-3	45688950	44977346	0.03	96.99	98.00	94.11

Transcriptome analysis of a stigma exsertion mutant in mungbean

RichHTML422

PDF

Abstract

Cite this article

share this article

Figures/Tables 10

References 25

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[2]	Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[3]	Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[4]	Cai Zhao-Qin, He Guan-Yong, He Wen, Ruan Li-Xia, Liang Zhen-Hua, Li Yong-Zhen, Li Heng-Rui, Chen Hui-Xian. Dynamic transcriptome analysis and key gene discovery during cassava branching development [J]. Acta Agronomica Sinica, 2026, 52(5): 1430-1441.
[5]	Zhang Xi, Wang Guang-En, Li Shao-Qi, Liu Yi, Li Jun-Lan, Qian Yu-Yuan. Transcriptome sequencing-based analysis on the formation mechanism of fiber micronaire differences between two sister lines derived from Gossypium hirsutum-G. barbadense hybrid [J]. Acta Agronomica Sinica, 2026, 52(5): 1442-1458.
[6]	Han Ya-Xin, He Guan-Hua, Zhang Xiao-Qiong, Zhang Deng-Feng, Li Yong-Xiang, Liu Xu-Yang, Wang Tian-Yu, Li Yu, Zou Hua-Wen, Li Chun-Hui. Identification of maize lateral root density genes resources through integrated RNA-seq and BSA-seq analyses [J]. Acta Agronomica Sinica, 2026, 52(5): 1341-1352.
[7]	Ma Liang, Ma Lu, Zhang Shu-Yu, Zhang Hui-Min, Wang Ren-Ming, Song Xu-Dong, Zhang Zhen-Liang, Mao Yu-Xiang, Lu Hu-Hua, Chen Guo-Qing, Hao De-Rong, Zhou Guang-Fei. Transcriptome analysis and identification of candidate genes associated with husk number in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 790-801.
[8]	Zhang Yu, Liu Fang, Cai Cheng-Cheng, Yang Xiao-Hua, Jia MO-Shi-Zha, Yang Yuan-Jun, Wang Xi-Yao. Preliminary investigation on the mechanism of potato tuber dormancy release induced by combined treatment of bromoethane and gibberellin [J]. Acta Agronomica Sinica, 2026, 52(3): 825-838.
[9]	Yu Yong-Chao, Liu Ming, Jin Rong, Zhao Peng, Zhang Qiang-Qiang, Wang Jing, Zhu Xiao-Ya, Tang Zhong-Hou. Physiological mechanism and transcriptome analysis of sweet potato overgrowth under high-nitrogen conditions [J]. Acta Agronomica Sinica, 2026, 52(3): 813-824.
[10]	Zhang Li-Lan, Yang Jun, Wang Rang-Jian. Identification of candidate genes related to glycoside aroma precursor content in tea plant using WGCNA [J]. Acta Agronomica Sinica, 2026, 52(2): 494-513.
[11]	Liu Di, Li Rui-Yuan, Shi Mao-Zhu, Li Hong-You, Chen Qing-Fu, Shi Tao-Xiong. Phenotypic characterization and transcriptomic analysis of the semi-dwarf mutant sd3 in Tartary buckwheat [J]. Acta Agronomica Sinica, 2026, 52(1): 316-328.
[12]	JI Bai-Lu, SUN Yi-Wen, LIU Wan-Feng, QIAN Ya-Xin, JIANG Cai-Hong, GENG Rui-Mei, LIU Dan, CHENG Li-Rui, YANG Ai-Guo, HUANG Li-Yu, LI Xiao-Xu, PU Wen-Xuan, GAO Jun-Ping, ZHANG Qiang, WEN Liu-Ying. Functional verification of the key gene NtLPAT involved in lipid biosynthesis in tobacco [J]. Acta Agronomica Sinica, 2025, 51(9): 2527-2537.
[13]	HE Hong-Li, ZHANG Yu-Han, YANG Jing, CHENG Yun-Qing, ZHAO Yang, LI Xing-Nuo, SI Hong-Liang, ZHANG Xing-Zheng, YANG Xiang-Dong. Creation and physiological analysis of an e1-as gene mutant in soybean [J]. Acta Agronomica Sinica, 2025, 51(8): 2228-2239.
[14]	YANG Xiao-Hui, YAN Xuan-Jun, YANG Wen-Yan, FU Jun-Jie, YANG Qin, XIE Yu-Xin. Effect evaluation and investigation on molecular mechanism of the ZmKL1 favorable allele in regulating maize kernel size [J]. Acta Agronomica Sinica, 2025, 51(6): 1501-1513.
[15]	WANG Xiao-Lin, LIU Zhong-Song, KANG Lei, YANG Liu. Mapping of silique length and seeds per silique and transcriptome profiling of pod walls in Brassica napus L. [J]. Acta Agronomica Sinica, 2025, 51(4): 888-899.