亚麻LuWRI1a在旱盐胁迫响应中的功能分析

doi:10.3724/SP.J.1006.2024.34171

Abstract

Abstract:

The AP2/ERF family of transcription factors is involved in the regulation of plant responses to biotic and abiotic stresses. Previously, we cloned LuWRI1a, a WRINKLED1 homologous gene from flax. Protein sequence analysis showed that LuWRI1a contained two AP2 DNA-binding domains and belonged to the AP2/ERF transcription factor family. The cis-acting elements of LuWRI1a were analyzed that pLuWRI1a was found containing multiple abiotic stress elements in response to light, drought, low temperature and hormones. In this study, the flax cultivar Longya 10 and LuWRI1a overexpression transgenic pure lines were used as the experimental materials, and salt stress and drought stress treatments were simulated with 200 mmol L^-1NaCl nutrient solution and 25% PEG nutrient solution. The results showed that the relative plant height, primary root length, lateral root number, and leaf number of transgenic plants were elevated after salt and drought stress treatments. The activities of three antioxidant enzymes were significantly higher than the control, while MDA content was lower. The relative expression levels of the abiotic stress-responsive genes, LuAREB, LuDREB, LuLEA, and LuNCED, were up-regulated. By exploring the biological function of LuWRI1a under adversity stress, it was found that LuWRI1a enhanced flax tolerance by resisting the inhibition of flax growth by salt stress and drought stress, enhancing the scavenging ability of reactive oxygen species, reducing the oxidative damage of membrane lipids, and activating the expression of adversity stress response genes. In summary, LuWRI1a may be a multifunctional gene, which was not only involved in fatty acid synthesis and metabolism pathway, but also may be involved in plant abiotic stress signaling pathway. This study provides a new genetic resource for the improvement of stress-tolerant varieties of flax.

Key words: flax, LuWRI1a, salt stress, drought stress, functional analysis

LI Wen-Juan, WANG Li-Min, QI Yan-Ni, ZHAO Wei, XIE Ya-Ping, DANG Zhao, ZHAO Li-Rong, LI Wen, XU Chen-Meng, WANG Yan, ZHANG Jian-Ping. Functional analysis of flax LuWRI1a in response to drought and salt stresses[J].Acta Agronomica Sinica, 2024, 50(7): 1750-1761.

Figures/Tables 9

Table 1

Fig. 1

Table 2

Predicted cis-acting elements of LuWRI1a gene promoter"

调控元件 Cis-element	核心序列 Sequence (5′-3′)	位置 Position	功能 Function
ACE	CTAACGTATT	-1239	光响应顺式作用元件 Cis-acting element involved in light responsiveness
AE-box	AGAAACAA	+144	光响应模块的组成部分 Part of a module for light response
ARE	AAACCA	-1057, -1801, +2406	厌氧诱导必需的顺式作用调控元件 Cis-acting regulatory element essential for the anaerobic induction
Box 4	ATTAAT	+579, -2178	光响应保守模块的一部分 Part of a conserved DNA module involved in light responsiveness
CGTCA-motif	CGTCA	+963, +1169	MeJA响应顺式作用元件 Cis-acting regulatory element involved in the MeJA-responsiveness
GT1-motif	GGTTAA(T)	+17, -679, -680	光响应元件 Light responsive element
Gap-box	CAAATGAA(A/G)A	+524, +1038	光响应元件的一部分 Part of a light responsive element
LTR	CCGAAA	+1983	低温响应顺式作用元件 Cis-acting element involved in low-temperature responsiveness
MBS	CAACTG	+2127	参与干旱诱导的MYB结合位点 MYB binding site involved in drought-inducibility
MBSI	aaaAaaC(G/C)GTTA	-2215	参与干旱诱导的MYB结合位点 MYB binding site involved in flavonoid biosynthetic genes regulation
MYB	CAACCA, TAACCA	-16, +391, +681, -759, +1853, +2105, +2123	干旱、盐、ABA 和低温响应 Drought, salt, ABA, and low-temperature responsive
MYC	CATTTG	+73, +267, -524, -569, -1038, -1363	干旱和ABA响应 Drought and ABA responsive
O2-site	GATGATGTGG	+350	参与玉米醇溶蛋白新陈代谢调节的顺式调控元件 Cis-acting regulatory element involved in zein metabolism regulation
STRE	AGGGG	+1024, -1548, -1580, +1604, +2307	由热激、渗透应激、低pH值和营养缺乏激活的元件 Activated by heat shock, osmotic stress, low pH, and nutrient starvation
ERE	ATTTTAAA, ATTTCATA	+1439, -1905	乙烯响应元件 Ethylene-responsive element
TCA-element	CCATCTTTTT	+2343	水杨酸响应的顺式作用元件 Cis-acting element involved in salicylic acid responsiveness
TGACG-motif	TGACG	-963, -1169	MeJA 响应顺式作用元件 Cis-acting regulatory element involved in the MeJA-responsiveness

Table 2

Fig. 2

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Fig. 6

Fig. 7

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