Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2025, Vol. 51 ›› Issue (10): 2681-2692.doi: 10.3724/SP.J.1006.2025.55019

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Comprehensive evaluation of alkaline tolerance in Brassica napus at the seedling stage

HU Zhi-Kang1(), SHU Yu1, WANG Hui1, YANG Ying-Ying1, LIAO Jun-Yu2, LIU Jia1, CHENG Hong-Tao1, GUO Chen3, ZHANG Yuan-Yuan1, LIU Sheng-Yi1, HU Qiong1, MEI De-Sheng1,*(), LI Chao1,*()   

  1. 1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, Hubei, China
    2The School of Life Science, Yangtze University, Jingzhou 434000, Hubei, China
    3Institute of Plant Protection, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, Inner Mongolia, China
  • Received:2025-03-20 Accepted:2025-07-09 Online:2025-10-12 Published:2025-07-22
  • Contact: *E-mail: lichao01@caas.cn;E-mail: meidesheng@caas.com E-mail:3014849124@qq.com;meidesheng@caas.com;lichao01@caas.cn
  • Supported by:
    National Science and Technology Innovation 2030 of China(2022ZD04010);National Natural Science Foundation of China(32372065);Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-OCRI);China Agriculture Research System of MOF and MARA(CARS-12)

RichHTML442

18

PDF

208

Abstract:

Soil salinization is a major factor limiting rapeseed production. Identifying alkali-tolerant rapeseed germplasm is of great importance for breeding programs, enhancing oil production capacity, and promoting the sustainable use of saline-alkali land. Accurate and efficient screening and identification methods are fundamental to the discovery of alkali-tolerant germplasm resources and the genetic improvement of alkali tolerance in rapeseed. In this study, a mixed alkali solution (75 mmol L-1, NaHCO3 : Na2CO3 at a molar ratio of 2:1) was used to simulate alkaline stress. Four soil pH gradients (pH 8.75, 8.95, 9.18, and 9.42) were established by adjusting the ratio of nutrient soil to vermiculite. The widely cultivated variety ‘Zhongshuang 11' was used as the test material to evaluate the effects of different pH levels on rapeseed growth and development. A soil pH of 8.95 was identified as optimal for evaluating alkali tolerance. A total of 224 natural rapeseed germplasms were subjected to alkali stress, and four phenotypic traits were measured under both control and alkaline conditions: aboveground fresh weight, aboveground dry weight, leaf number, and survival rate. Alkali tolerance at the seedling stage was comprehensively assessed using coefficient of variation analysis, correlation analysis, principal component analysis, membership function analysis, and cluster analysis. Results showed that under pH8.95 treatment, the coefficient of variation (CV) for all phenotypic traits increased significantly compared to control conditions, with the CV of both aboveground fresh and dry weights increasing by more than 25%. A highly significant positive correlation was observed between the alkali tolerance indices (aboveground fresh/dry weight, leaf number, survival rate) and the comprehensive alkali tolerance score (D-value). The first two principal components (PC1 and PC2), along with the comprehensive score D, followed a normal distribution. Based on membership function and cluster analyses, the 224 germplasms were classified into five alkali tolerance groups: highly alkali-sensitive (7.14%), alkali-sensitive (50.00%), intermediate (33.93%), alkali-tolerant (8.58%), and highly alkali-tolerant (0.45%). In total, 19 alkali-tolerant and 1 highly alkali-tolerant germplasms were identified. A regression equation was established based on the tolerance indices of the four phenotypic traits across all samples: Y = 0.009 + 0.986X1 + 0.208X2 + 0.151X3. Aboveground fresh weight, dry weight, and leaf number were identified as key indicators for evaluating alkali tolerance in rapeseed seedlings.

Key words: Brassica napus (rapeseed), seedling stage, alkali tolerance of soil culture, evaluation and identification, germplasm screening

Table 1

Preparation of alkaline soil mixtures with different pH levels"

处理
Treatment		 营养土添加量
Nutrient soil addition (g kg-1)		 蛭石添加量
Vermiculite addition
(g kg-1)
CK 500 500
pH 8.75 600 400
pH 8.95 500 500
pH 9.18 400 600
pH 9.42 200 800

Fig. 1

Effects of different soil pH on seedling growth of rapeseed Zhuangshuang 11 Treatments are the same as those given in Table 1. A: phenotypic images of Zhuangshuang 11 under different soil pH conditions after three weeks growth. B: statistical analysis of aboveground fresh weight, aboveground dry weight, number of leaves, survival rate and germination rate of Zhuangshuang 11 after three weeks under different treatments. *, **, ***, and **** indicate significant differences at P < 0.05, P < 0.01, P < 0.001, and P < 0.0001, respectively."

Table 2

Phenotypic variation analysis of 224 rapeseed germplasms under pH8.95 treatment at the seedling stage"

性状
Trait		 处理
Treatment		 均值
Average		 标准差
SD		 变异系数
CV (%)		 最小值
Min.		 最大值
Max.
地上部鲜重
Shoot fresh weight (g plant-1)		 CK 1.87 0.78 41.49 0.40 3.86
pH 8.95 0.35 0.24 70.41 0 1.38
地上部鲜重耐碱指数RSFW 0.20 0.12 63.08 0 0.65
地上部干重
Shoot dry weight (g plant-1)		 CK 0.24 0.09 36.26 0.04 0.54
pH 8.95 0.05 0.03 68.22 0 0.21
地上部干重耐碱指数RSDW 0.21 0.15 70.40 0 0.92
叶片数
Number of leaves		 CK 3.85 0.72 18.65 2.22 5.83
pH 8.95 2.65 0.83 31.40 0 4.67
叶片数耐碱指数LR 0.70 0.21 30.20 0 1.00
存活率
Number of survivors (%)		 CK 100.00 0 0 100.00 100.00
pH8.95 59.77 26.77 44.78 0 100.00
存活率耐碱指数 SVR 0.60 0.27 44.78 0 1.00

Fig. 2

Correlation analysis between alkali tolerance index and D-value of 224 rapeseed materials ** indicates significant correlation at P < 0.01. Red indicates a positive correlation and blue indicates a negative correlation. RSFW: alkali tolerance index of aboveground fresh weight; RSDW: alkali tolerance index of aboveground dry weight; LR: alkali tolerance index of leaf number; SVR: alkali tolerance index of survival rate; D-value: comprehensive evaluation value."

Table 3

PCA analysis of 224 rapeseed germplasms under pH 8.95 treatment at seedling stage"

项目
Item		 主成分 Principal component
PC1 PC2 PC3 PC4
载荷因子Load factor 地上部鲜重耐碱指数RSFW 0.85 -0.28 0.25 -0.37
地上部干重耐碱指数RSDW 0.78 -0.51 0.01 0.36
叶片数量耐碱指数LR 0.81 0.24 -0.52 -0.08
存活率耐碱指数SVR 0.68 0.65 0.31 0.15
特征值Eigenvalue 2.45 0.82 0.43 0.30
贡献率Contribution (%) 61.26 20.56 10.77 7.41
累计贡献率Cumulative contribution (%) 61.26 81.82 92.59 100.00

Fig. 3

Frequency distribution of principal components and comprehensive evaluation D-value for alkaline tolerance indices A: PC1 frequency distribution of alkali tolerance indices of four phenotypic indices; B: PC2 frequency distribution of alkali tolerance indices of four phenotypic indices; C: frequency distribution of comprehensive evaluation D-value."

Fig. 4

Cluster analysis of 224 rapeseed materials based on D-values The D-value is a comprehensive evaluation value obtained by membership function analysis. According to the D-value, 224 B. napus materials were clustered into five categories. Grade I was alkali extremely sensitive type, grade II was alkali sensitive type, grade III was intermediate tolerance type, grade IV was alkali tolerance type, and grade V was alkali high tolerance type."

Fig. 5

Phenotypic comparison of alkaline-tolerance and sensitive materials A: No. 103, 113 are alkali sensitive materials; B: No. 210, 211 are alkali tolerant materials."

[1] 刘奕媺, 于洋, 方军. 盐碱胁迫及植物耐盐碱分子机制研究. 土壤与作物, 2018, 7: 201-211.
Liu Y M, Yu Y, Fang J. Saline-alkali stress and molecular mechanism of saline-alkali tolerance in plants. Soils Crops, 2018, 7: 201-211 (in Chinese with English abstract).
[2] Zhang H L, Yu F F, Xie P, Sun S Y, Qiao X H, Tang S Y, Chen C X, Yang S, Mei C, Yang D K, et al. A Gγ protein regulates alkaline sensitivity in crops. Science, 2023, 379: eade8416.
[3] Munns R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol, 2008, 59: 651-681.
doi: 10.1146/annurev.arplant.59.032607.092911 pmid: 18444910
[4] Ren Z H, Gao J P, Li L G, Cai X L, Huang W, Chao D Y, Zhu M Z, Wang Z Y, Luan S, Lin H X. A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nat Genet, 2005, 37: 1141-1146.
[5] Cao Y B, Liang X Y, Yin P, Zhang M, Jiang C F. A domestication-associated reduction in K+-preferring HKT transporter activity underlies maize shoot K+ accumulation and salt tolerance. New Phytol, 2019, 222: 301-317.
[6] Cao Y B, Zhang M, Liang X Y, Li F R, Shi Y L, Yang X H, Jiang C F. Natural variation of an EF-hand Ca2+-binding-protein coding gene confers saline-alkaline tolerance in maize. Nat Commun, 2020, 11: 186.
[7] 陈二影, 王润丰, 秦岭, 杨延兵, 黎飞飞, 张华文, 王海莲, 刘宾, 孔清华, 管延安. 谷子芽期耐盐碱综合鉴定及评价. 作物学报, 2020, 46: 1591-1604.
doi: 10.3724/SP.J.1006.2020.04064
Chen E Y, Wang R F, Qin L, Yang Y B, Li F F, Zhang H W, Wang H L, Liu B, Kong Q H, Guan Y A. Comprehensive identification and evaluation of foxtail millet for saline-alkaline tolerance during germination. Acta Agron Sin, 2020, 46: 1591-1604 (in Chinese with English abstract).
[8] 张静, 高文博, 晏林, 张宗文, 周海涛, 吴斌. 燕麦种质资源耐盐碱性鉴定评价及耐盐碱种质筛选. 作物学报, 2023, 49: 1551-1561.
doi: 10.3724/SP.J.1006.2023.21032
Zhang J, Gao W B, Yan L, Zhang Z W, Zhou H T, Wu B. Identification and evaluation of salt-alkali tolerance and screening of salt-alkali tolerant germplasm of oat (Avena sativa L.). Acta Agron Sin, 2023, 49: 1551-1561 (in Chinese with English abstract).
[9] 薛天源, 鲁金春子, 何思晓, 余忆, 陈敬东, 文静, 沈金雄, 傅廷栋, 曾长立, 万何平. 286份甘蓝型油菜种质苗期耐盐碱性综合评价. 植物遗传资源学报, 2024, 25: 356-372.
doi: 10.13430/j.cnki.jpgr.20230827002
Xue T Y, Lu J C Z, He S X, Yu Y, Chen J D, Wen J, Shen J X, Fu T D, Zeng C L, Wan H P. Comprehensive evaluation on saline-alkali tolerance of 286 Brassica napus germplasm at seedling stage. J Plant Genet Resour, 2024, 25: 356-372 (in Chinese with English abstract).
[10] 沈金雄, 傅廷栋. 我国油菜生产、改良与食用油供给安全. 中国农业科技导报, 2011, 13(1): 1-8.
Shen J X, Fu T D. Rapeseed production, improvement and edible oil supply in China. J Agric Sci Technol, 2011, 13(1): 1-8 (in Chinese with English abstract).
[11] 冯海棠, 王汉中. 新形势下的我国食用植物油供给安全对策. 中国油料作物学报, 2024, 46: 221-227.
doi: 10.19802/j.issn.1007-9084.2024021
Feng H T, Wang H Z. Security strategy for the nation's edible vegetable oil supplies under the new circumstances. Chin J Oil Crop Sci, 2024, 46: 221-227 (in Chinese with English abstract).
[12] 杨明煊, 李明玉, 汪波, 王泽, 刘智强, 周广生, 于放, 刘志文. 甘蓝型油菜转录因子BnHY5-2参与盐碱胁迫的响应. 华北农学报, 2024, 39(4): 47-53.
doi: 10.7668/hbnxb.20194546
Yang M X, Li M Y, Wang B, Wang Z, Liu Z Q, Zhou G S, Yu F, Liu Z W. Response of the transcription factor BnHY5-2 in Brassica napus L. to salt and alkali stress. Acta Agric Boreali-Sin, 2024, 39(4): 47-53 (in Chinese with English abstract).
[13] 汪波, 文静, 张凤华, 李立军, 来永才, 任长忠, 鲁剑巍, 沈金雄, 郭亮, 周广生, 等. 耐盐碱油菜品种选育及修复利用盐碱地研究进展. 科技导报, 2021, 39(23): 59-64.
doi: 10.3981/j.issn.1000-7857.2021.23.009
Wang B, Wen J, Zhang F H, Li L J, Lai Y C, Ren C Z, Lu J W, Shen J X, Guo L, Zhou G S, et al. Research progress in breeding of saline-alkaline tolerant rapeseed and restoring the salinate land. Sci Technol Rev, 2021, 39(23): 59-64 (in Chinese with English abstract).
doi: 10.3981/j.issn.1000-7857.2021.23.009
[14] 万何平, 张浩, 余忆, 陈敬东, 曾长立, 赵伦, 文静, 沈金雄, 傅廷栋. 油菜耐盐碱研究与应用. 中国农业科技导报, 2022, 24(12): 59-67.
doi: 10.13304/j.nykjdb.2022.0970
Wan H P, Zhang H, Yu Y, Chen J D, Zeng C L, Zhao L, Wen J, Shen J X, Fu T D. Study and application of salt and alkali tolerance in rapeseed. J Agric Sci Technol China, 2022, 24(12): 59-67 (in Chinese with English abstract).
[15] Zhang Y Y, Yang Z Q, He Y Z, Liu D X, Liu Y Y, Liang C Y, Xie M L, Jia Y P, Ke Q L, Zhou Y M, et al. Structural variation reshapes population gene expression and trait variation in 2105 Brassica napus accessions. Nat Genet, 2024, 56: 2538-2550.
[16] 梁潇, 侯向阳, 王艳荣, 任卫波, 赵青山, 王照兰, 李怡, 武自念. 羊草种质资源耐盐碱性综合评价. 中国草地学报, 2019, 41(3): 1-9.
Liang X, Hou X Y, Wang Y R, Ren W B, Zhao Q S, Wang Z L, Li Y, Wu Z N. Comprehensive evaluation on saline-alkali tolerance of Leymus chinensis germplasm resources. Chin J Grassland, 2019, 41(3): 1-9 (in Chinese with English abstract).
[17] 李建波, 刘志萍, 王文迪, 巴图, 马宇, 吕二锁, 杜慧婷, 徐寿军. 大麦萌发期优异耐碱性种质筛选与鉴定. 草业科学, 2023, 40: 2581-2597.
Li J B, Liu Z P, Wang W D, Ba T, Ma Y, Lyu E S, Du H T, Xu S J. Screening and identification of excellent alkali-tolerant germplasm of barley at the germination stage. Pratac Sci, 2023, 40: 2581-2597 (in Chinese with English abstract).
[18] Oster J D, Shainberg I, Abrol I P. Reclamation of salt-affected soils. In: SkaggsR W, vanSchilfgaarde J, AgriculturalDrainage. MadisonWI, Crop Science Society of America, Soil Science Society of America,eds. USA: American Society of Agronomy, 2015. pp 659-691.
[19] Zhang L, Qian Q, Song S Y. Combating alkaline stress with Alkaline tolerance 1, which encodes a conserved Gγ protein in multiple crops. Plant Commun, 2023, 4: 100603.
[20] 李积铭, 李爱国, 李和平, 管明威, 武军艳, 孙万仓, 翟兰菊, 马骊, 郭安强. 环渤海区域耐盐碱冬油菜品种全生育期鉴定筛选. 中国油料作物学报, 2025, 47: 402-412.
doi: 10.19802/j.issn.1007-9084.2024039
Li J M, Li A G, Li H P, Guan M W, Wu J Y, Sun W C, Zhai L J, Ma L, Guo A Q. Identification and screening of saline-alkali tolerant winter Brassica napus L. cultivars during whole growth stage in the Bohai Rim Region. Chin J Oil Crop Sci, 2025, 47: 402-412 (in Chinese with English abstract).
[21] 郭凯, 封晓辉, 伍靖伟, 陈小兵, 巨兆强, 孙宏勇, 刘小京. 盐碱地肥沃耕层构建水肥盐综合调控机理与技术研究进展. 土壤学报, 2024, 61: 29-38.
Guo K, Feng X H, Wu J W, Chen X B, Ju Z Q, Sun H Y, Liu X J. Research progress on mechanism and technology of integrated regulating on soil water, salt and fertility under fertile plough layers construction in saline-alkali soils. Acta Pedol Sin, 2024, 61: 29-38 (in Chinese with English abstract).
[22] 王思宇.大豆种子萌发期耐碱种质资源筛选与转录组研究. 吉林农业大学硕士学位论文, 吉林长春, 2024.
Wang S Y. Selection of Alkali-tolerant Germplasm Resources and Transcriptome Studies during Germination of Soybean. MS Thesis of Jilin Agricultural University, Changchun, Jilin, China, 2024 (in Chinese with English abstract).
[23] 相广庆.VvERF1B促进草酸分泌提高葡萄根系碱性盐耐性的分子机制. 山东农业大学博士学位论文, 山东泰安, 2024.
Xiang G Q.Molecular Mechanisms Underlying VvERF1B Promoting Oxalate Acid Secretion and Improving Alkaline Salt Tolerance in Grapevine Roots. PhD Dissertation of Shandong Agricultural University, Tai'an, Shandong, China, 2024 (in Chinese with English abstract).
[24] Sheng W, Zhang G G, Zhai L Y, Xu J L. Candidate genes for alkali tolerance identified by genome-wide association study at the seedling stage in rice (Oryza sativa L.). Sci Rep, 2024, 14: 30063.
[25] Chen J D, Zhang H, Tong J Q, Liu C Y, Ran J H, Tang J, Liu J Y, Wen J, Zeng C L, Wan H P, et al. Genome-wide association analysis of root length traits in Brassica napus at germination stage under sodium carbonate stress. Euphytica, 2021, 217: 197.
[26] 陶顺仙, 张燕, 李萍, 燕佳琦, 徐爱遐, 黄镇. NaHCO3胁迫对甘蓝型油菜幼苗生长的影响及其耐盐候选基因预测. 西北农业学报, 2019, 28: 1978-1986.
Tao S X, Zhang Y, Li P, Yan J Q, Xu A X, Huang Z. Effects of NaHCO3 stress on growth of Brassica napus seedlings and prediction of candidate genes related to salt tolerance. Acta Agric Boreali-Occident Sin, 2019, 28: 1978-1986 (in Chinese with English abstract).
[27] Guo S Q, Chen Y X, Ju Y L, Pan C Y, Shan J X, Ye W W, Dong N Q, Kan Y, Yang Y B, Zhao H Y, et al. Fine-tuning gibberellin improves rice alkali-thermal tolerance and yield. Nature, 2025, 639: 162-171.
[1] Yao Shu, Guo Kai-Yue, Zhai Hui-Hui, Yao Jia-Hui, Deng Wen-Qi, Yan Ling, Huang Chi, Gao Yang, Yu Yan-Ran, Zhao Zhen-Bang, Li Ying-Hui, Wang Xiao-Bo, Li Jia-Jia. Comprehensive evaluation of low-iron tolerance and screening of elite germplasm at the soybean seedling stage [J]. Acta Agronomica Sinica, 2026, 52(5): 1373-1387.
[2] Qi Qing-Song, Niu Xiang-Yu, Liu Bing-Ke, Kang Lu, Wang Chen, Feng De-Shun. Salt tolerance identification, screening and salt tolerance index evaluation of wheat-Thinopyrum intermedium radiation mutagenesis germplasm at germination and seedling stage [J]. Acta Agronomica Sinica, 2026, 52(2): 389-404.
[3] Li Shi-Qing, Wang Qian, Wang Su-Hua, Zhang Yao-Wen, Wang Li-Xia. Evaluation of salt tolerance at the seedling stage and related gene mining in mung bean germplasm resources [J]. Acta Agronomica Sinica, 2026, 52(2): 376-388.
[4] Wang Fei-Fei, Zhang Sheng-Zhong, Yang Gui-Hua, Miao Hua-Rong, Hu Xiao-Hui, Zhang Ze-Lin, Liu Sha-Sha, Qiao Li-Xian, Shan Shi-Hua, Chen Jing. Comprehensive evaluation of salt tolerance and identification of elite salt-tolerant germplasm in 331 peanut accessions at seedling stage [J]. Acta Agronomica Sinica, 2026, 52(1): 279-294.
[5] HU Run-Hui, WANG Jun-Cheng, SI Er-Jing, ZHANG Hong, LI Xing-Mao, MA Xiao-Le, MENG Ya-Xiong, WANG Hua-Jun, LIU Qing, YAO Li-Rong, LI Bao-Chun. Screening of drought and salt tolerant germplasm during wheat seedling stage and comprehensive evaluation of drought and salt tolerance [J]. Acta Agronomica Sinica, 2025, 51(9): 2371-2386.
[6] MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[7] MA Qun, WANG Zhi-Hao, YAN Lei, LI Yu-Jiao, WANG Jia-Qi, LI Zhao, LIU Wei, AI Xin, MA Qian-Chi, WANG Xiao-Guang, ZHONG Chao, REN Jing-Yao, LIU Xi-Bo, ZHAO Shu-Li, ZHANG He, ZHAO Xin-Hua, JIANG Chun-Ji, WANG Jing, YU Hai-Qiu. Screening and evaluation system for drought resistance in high-oleic acid and common peanut at the germination stage [J]. Acta Agronomica Sinica, 2025, 51(12): 3266-3280.
[8] GE Jia-Hao, LEI Xin-Yue, WANG Qing-Ming, HAN Hui-Bing, LI Shao-Fei, WANG Qi-Xuan, FENG Bai-Li, GAO Jin-Feng. Screening of low-phosphorus tolerant germplasm and comprehensive evaluation of low phosphorus tolerance in Tartary buckwheat at seedling stage [J]. Acta Agronomica Sinica, 2025, 51(11): 2911-2922.
[9] SUN Xian-Jun, HU Zheng, JIANG Xue-Min, WANG Shi-Jia, CHEN Xiang-Qian, ZHANG Hui-Yuan, ZHANG Hui, JIANG Qi-Yan. Identification, evaluation and screening of salt-tolerant of soybean germplasm resources at seedling stage [J]. Acta Agronomica Sinica, 2024, 50(9): 2179-2186.
[10] CHEN Jia-Ting, BAI Xin, GU Yu-Jie, ZHANG Xiao-Wen, GUO Hui-Juan, CHANG Li-Fang, CHEN Fang, ZHANG Shu-Wei, ZHANG Xiao-Jun, LI Xin, FENG Rui-Yun, CHANG Zhi-Jian, QIAO Lin-Yi. Applicability evaluation of screen methods to identify salt tolerance in wheat at germination and seedling stages [J]. Acta Agronomica Sinica, 2024, 50(5): 1193-1206.
[11] LI Yun-Xiang, ZHANG Si-Tian, HOU Wan-Wei, ZHANG Xiao-Juan. Drought resistance identification and SNP association analysis of wheat germplasm introduced by ICARDA at seedling stage [J]. Acta Agronomica Sinica, 2024, 50(11): 2742-2753.
[12] SUN Xian-Jun, JIANG Qi-Yan, HU Zheng, LI Hong-Bo, PANG Bin-Shuang, ZHANG Feng-Ting, ZHANG Sheng-Quan, ZHANG Hui. Identification and evaluation of wheat germplasm resources at seedling stage [J]. Acta Agronomica Sinica, 2023, 49(4): 1132-1139.
[13] LI Ji-Jun, CHEN Ya-Hui, WANG Yi-Jin, ZHOU Zhi-Hua, GUO Zi-Yue, ZHANG Jian, TU Jin-Xing, YAO Xuan, GUO Liang. Evaluation of field waterlogging tolerance and selection of waterlogging-resistant germplasm resources of Brassica napus L. [J]. Acta Agronomica Sinica, 2023, 49(12): 3162-3175.
[14] ZHAO Peng, CHEN Guang-Xia, ZHANG Yan-Ping, YANG Xiao-Hui, LIU Fang, DONG Dao-Feng. Alkaline tolerance identification method of potato seedlings and comprehensive assessment of alkaline tolerance of 86 kinds of potato germplasms [J]. Acta Agronomica Sinica, 2023, 49(11): 2923-2934.
[15] CAO Hui-Min, YANG Xian-Li, WANG Li-Zhi, LI Ping-Ping, ZHAI Lai-Yuan, JIANG Shu-Kun, ZHENG Tian-Qing, QIU Xian-Jin, XU Jian-Long. QTL identification and favorable allele mining of cold tolerance at seedling stage by reciprocal introgression and recombinant inbred line populations in rice [J]. Acta Agronomica Sinica, 2023, 49(10): 2633-2642.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd