Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1513-1519.doi: 10.3724/SP.J.1006.2014.01513

• RESEARCH NOTES • Previous Articles    

Double Fertilization and Duration of Phases in Peanut (Arachis hgpogaea L.)

SHI Chun-Yan,SHEN Jia-Heng*,LI Wei   

  1. College of Life Science and Technology, Harbin Normal University, Harbin 150080, China
  • Received:2013-12-02 Revised:2014-04-16 Online:2014-08-12 Published:2014-05-16
  • Contact: 申家恒, E-mail: hsdshenjiaheng@163.com, Tel: 0535-6685930

Abstract:

 We used paraffin section technology to observe fertilization and duration of phased in fertilization of Arachis hypogaea L. The results indicated that reproductive cell in the pollen came into mitosis tube one hour after bloom, the mitosis lasted about three hours. Sperms released to embryonary sac at about eight hours after flowering. Sperm and egg cell or polar nucleus or secondary nucleus fused from nine to fifteen hours after flowering. Fusion needed about four hours. There were obvious isometrical two nuclei in the zygote at 30 hours after the two nucleole fusion, which came into division stage. Most of zygote had transverse division, and a few longitudinal division. First division needed about 36 hours after bloom, then formed two-cell proembryo and zygote had a dormant period for about 19 hours. After polar nucleus or secondary nucleus fertilization, almost the fusion completed in 13 hours after bloom, and male nucleolus appeared in one of polar nucleus or secondary nucleus, forming primary endosperm nucleus. The primary endodperm nucleus started division without domancy or after short dormant period in 15 hours after bloom. There were more sperms coming into embryonary sat and also single fertilization phenomenon.This study provides a understanding for morphological changed and corresponding timing of male and female cells in peanut fertiligation, confirms the duration of zygote dormancy, and enriches thepeanut embryological information, which has great significance for peanut breeding and genetically modified (gm) operation.

Key words: Arachis hypogaea L., Fertilization, Zygocyte, Zygote dormancy, Primary endosperm nucleus

[1]杨衔晋, 黄普华, 付沛云, 李冀云, 陈佑安, 李树刚, 张本能, 韦裕宗, 黄德爱, 卫兆芬, 吴德林, 卫思奇. 中国植物志(第41卷). 北京: 科学出版社, 1995. pp 361–362



Yang X J, Huang P H, Fu P Y, Li J Y, Chen Y A, Li S G, Zhang B N, Hui Y Z, Huang D A, Wei Z F, Wu D W, Wei S Q. Flora China, (Vol. 41). Beijing: Science Press, 1995. pp 361–362 (in Chinese)



[2]李明姝, 姚开, 贾冬英, 何强, 赖本丽. 花生功能成分及其综合利用. 中国油脂, 2004, 29(9): 13–15



Li M S, Yao Kai, Jia D Y, He Qiang, Lai B L. Functional components in peanut and their comprehensive utilization. China Oils Fats, 2004, 29(9): 13–15 (in Chinese with English abstract)



[3]王福青. 花生大孢子发生过程的观察. 莱阳农学院学报, 1991, 8: 119–112



Wang F Q. To observe Megasporogenesis development in Arachis hypogea . J Laiyang Agric Coll, 1991, 8: 119–112 (in Chinese)



[4]汤泽生, 王祖秀, 杜素琼, 梁素华, 刘钰. 花生小孢子发育的初步研究. 中国油料, 1982, (1): 38–42



Tang Z S, Wang Z X, Du S Q, Liang S H, Liu Y. Preliminary study microspore development in Arachis hypogeae. Zhongguo Youliao, 1982, (1): 38–42 (in Chinese)



[5]王福青, 王铭伦, 丛明日. 郑芝荣. 花生雌雄两性细胞发育关系的研究. 植物学通报, 2000, 17: 366–371



Wang F Q, Wang M L, Cong M R, Zheng Z R. Research on the relation between the development of peanut female and male tissues and cells. Chin Bull Bot, 2000, 17: 366–371 (in Chinese with English abstract)



[6]邢树平, 席湘媛. 花生双受精作用的研究. 山东农业大学学报, 1996, 27: 33–38



Xing S P, Xi X Y. fertilization in Arachis hypogeae L. J Shandong Agric Univ, 1996, 27: 33–38 (in Chinese with English abstract)



[7]Smith B W. Arachis hypogeae embryogeny and the effect of peg elongation upon embryo and endosperm growth. Am J Bot, 1956, 43: 233–240



[8]何远康, 丘运兰, 韩惠珍. 花生胚胎发育的形态学观察. 华南农业大学学报, 1992, 13(2): 34–39



He Y K, Qiu Y L, Han H Z. Morphological observation of peanut (Arachis hypogeae L.) embryonic development. J South China Agric Univ, 1992, 13(2): 34–39 (in Chinese with English abstract)



[9]Maheshwari P. An intruduction to the embryology of angiosperms. New York: McGraw Hill Book Comp, 1950. pp 162–176



[10]申家恒. 大豆受精作用的研究. 植物学报, 1983, 25: 213–219



Seng J H. Study on the fertilization in Glycine max. Acta Bot Sin, 1983, 25: 213–219 (in Chinese with English abstract)



[11]孙大荣. 花生育种学. 北京: 中国农业出版社, 1998. pp 75–79



Sun D R. Science of breeding in Arachis hypogeae. Beijing: China Agriculture Press, 1998. pp 75–79 (in Chinese)



[12]南京农学院, 江苏农学院. 作物栽培学(南方本下册). 上海: 上海科学技术出版社, 1981. pp 256–261



Nanjing Agricultural University, Jiangsu Agricultural University. Crop Cultivation (Part II for South Version). Shanghai: Shanghai Scientific & Technical Publishers, 1981. pp 256–261 (in Chinese)



[13] Johri B M, Ambegaokar K B, Srivastava P S. Comparative embryology of angiosperms. Berlin, Germany: Springer-Verlag, 1992. pp 383–391



[14]申家恒, 李慧蓉, 李玉芬, 殷华, 连永权. 玉米双受精的细胞学观察. 植物学报, 1987, 29: 480–485



Shen J H, Li H R, Li Y F, Yin Ha, Lian Y Q. Cytologic observations on the double fertilization in maize. Acta Bot Sin, 1987, 29: 480–485 (in Chinese with English abstract)

[1] Ma Hai-Hui, Zhang Guo-Ping, Yang Si-Cun, Wang Hong-Li. Effects of nitrogen application at different densities on carbon and nitrogen accumulation and translocation characteristics in forage maize in semi-arid regions [J]. Acta Agronomica Sinica, 2026, 52(4): 1193-1207.
[2] Liu Ning, Fan Ping, Wang Cheng, Chen Qi-Qi, Cheng Qing-Yue, Tie Xia-Na, Tang Jing-Sha, Liu Bin-Bin, Xie Hong-Kun, Wang Jia-Yue, Shi Yuan-Qing, Ma Jun. Effects of reduced nitrogen application combined with organic fertilizer on yield formation and nitrogen utilization in mechanically transplanted rice [J]. Acta Agronomica Sinica, 2026, 52(3): 866-880.
[3] LI Qiu-Yun, LI Shi-Gui, FAN Jun-Liang, LIU Hao-Tian, ZHAO Xiao-Bin, LYU Shuo, WANG Yan-Hao, YUE Yun, ZHANG Ning, SI Huai-Jun. Effects of ionic zinc and nano-zinc on physiological characteristics, yield, and quality of potato [J]. Acta Agronomica Sinica, 2025, 51(7): 1838-1849.
[4] LI Bing-Lin, YE Xiao-Lei, XIAO Hong, XIAO Guo-Bin, LYU Wei-Sheng, LIU Jun-Quan, REN Tao, LU Zhi-Feng, LU Jian-Wei. Effects of magnesium fertilization rates on rapeseed yield, magnesium uptake, and yield loss caused by frost damage [J]. Acta Agronomica Sinica, 2025, 51(7): 1850-1860.
[5] JIANG Yu-Zhou, WANG Jia, ZHANG Hong-Yuan, FENG Wen-Hao, WANG Peng, LI Yu-Yi. Effects of combined application of chemical fertilizer and organic materials on the soil bacterial and fungal community structure in maize fields [J]. Acta Agronomica Sinica, 2025, 51(5): 1378-1388.
[6] QIN Jin-Hua, HONG Wei-Yuan, FENG Xiang-Qian, LI Zi-Qiu, ZHOU Zi-Yu, WANG Ai-Dong, LI Rui-Jie, WANG Dan-Ying, ZHANG Yun-Bo, CHEN Song. Analysis of agronomic and physiological indicators of rice yield and grain quality under nitrogen fertilization management [J]. Acta Agronomica Sinica, 2025, 51(2): 485-502.
[7] WANG Zi-Lin, GUAN Pei-Yi, HUANG Cui, CHEN Jian, FANG Jia-Chuang, LIU Chen-Rui, GUO Zhang-Xi, WANG Zi-Ming, WANG Zhao-Hui, LIU Jin-Shan, TIAN Hui, SHI Mei. Responses of uptake and accumulation of manganese and zinc in wheat to zinc fertilization [J]. Acta Agronomica Sinica, 2025, 51(10): 2836-2842.
[8] XU Yi-Fan, XU Cai-Long, LI Rui-Dong, WU Zong-Sheng, HUA Jian-Xin, YANG Lin, SONG Wen-Wen, WU Cun-Xiang. Deep side fertilization improved soybean yield by optimizing leaf function and nitrogen accumulation [J]. Acta Agronomica Sinica, 2024, 50(9): 2335-2346.
[9] LIANG Jin-Yu, YIN Jia-De, HOU Hui-Zhi, XUE Yun-Gui, GUO Hong-Juan, WANG Shuo, ZHAO Qi-Zhi, ZHANG Xu-Cheng, XIE Jun-Hong. Effects of deep fertilization on ecological stoichiometric characteristics and photosynthetic carbon assimilation of flag leaves of spring wheat under drought conditions [J]. Acta Agronomica Sinica, 2024, 50(8): 2078-2090.
[10] GUO Song, GUO Hui-Ting, ZHANG Yu-Liang, QIAN Zi-Hui, WANG Zi-Jun, LU Jia-Ming, WANG Yuan, ZHAO Can, WANG Wei-Ling, ZHANG Hong-Cheng, YANG Feng-Ping, HUO Zhong-Yang. Effects of side deep placement of controlled release nitrogen management on rice yield, NH3, and greenhouse gas emissions [J]. Acta Agronomica Sinica, 2024, 50(6): 1525-1539.
[11] JIANG Da-Ren, XIONG Ruo-Yu, WU Jia-Qing, MAO Fu-Qin, FENG Jun-Jie, TAO Lei, XIE Xiao-Bing, PAN Xiao-Hua, ZENG Yong-Jun, WANG Ya-Liang, ZENG Yan-Hua. Effects of side-deep fertilization on yield formation and nitrogen absorption of high-quality early indica rice under precision sowing in line and machine planting [J]. Acta Agronomica Sinica, 2024, 50(12): 3096-3106.
[12] ZHAO Wei, HU Xiao-Na, ZHENG Yan, LIANG Na, ZHENG Bin, WANG Xiao-Xiao, WANG Jiang-Tao, LIU Ling, FU Guo-Zhan, SHI Zhao-Yong, JIAO Nian-Yuan. Characteristics of AMF community in maize and peanut rhizosphere soil and its response to phosphate application [J]. Acta Agronomica Sinica, 2024, 50(11): 2896-2907.
[13] LU Jian-Xiang, GAO Qian-Wen, GAO Zhi-Qiang, YANG Hui-Bing, WEN Shuang-Ya, SHI Nan, HU Wen-Rui, JIN Yu-Hao, CHEN Long, LIU Yun, CAO Zheng-Deng-Yuan. Effect of high-density planting and fertilizer reduction on yield and growth and development of indica hybrid rice [J]. Acta Agronomica Sinica, 2024, 50(10): 2586-2598.
[14] ZHANG Chen-Hui, ZHANG Yan, LI Guo-Hui, YANG Zi-Jun, ZHA Ying-Ying, ZHOU Chi-Yan, XU Ke, HUO Zhong-Yang, DAI Qi-Gen, GUO Bao-Wei. Root morphology and physiological characteristics for high yield formation under side-deep fertilization in rice [J]. Acta Agronomica Sinica, 2023, 49(4): 1039-1051.
[15] WANG Jin-Song, BAI Ge, ZHANG Yan-Hui, SHEN Tian-Yu, DONG Er-Wei, JIAO Xiao-Yan. Impacts of long-term fertilization on post-anthesis leaf senescence, antioxidant enzyme activities and yield in sorghum [J]. Acta Agronomica Sinica, 2023, 49(3): 845-855.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!