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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (07): 1127-1135.doi: 10.3724/SP.J.1006.2015.01127

• RESEARCH NOTES • Previous Articles     Next Articles

Screening of Differentially Expressed Genes and Analysis of Plant Hormones Related Genes under Water Stress in Sugarcane

LI Chang-Ning,XIE Jin-Lan,WANG Wei-Zan,LIANG Qiang,LI Yi-Jie,DONG Wen-Bin,LIU Xiao-Yan,YANG Li-Tao*,LI Yang-Rui*   

  1. Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China
  • Received:2014-12-08 Revised:2015-05-04 Online:2015-07-12 Published:2015-05-15

Abstract:

Sugarcane is an increasingly economically and environmentally important C4 crop. Water stress limits enormously sugarcane productivity worldwide, and understanding the molecular mechanisms for sugarcane stress responses will be useful for sugarcane improvement by genetic manipulation. To investigate the transcriptome changes in response to water stress, we used microarrays to profile expressions of 15 593 genes in sugarcane exposed to drought. The results indicated that 300 and 853 differentially expressed genes were detected under moderate and severe water stresses, respectively. The expression of differentially expressed genes treated with moderate water stress was mainly up-regulated, however that treated with severe water stress was mainly down-regulated. To further characterize these genes, we used Gene Ontology (GO) for their annotation, the results showed that differentially expressed genes possessed the functions of binding, transporter, molecular transducer and catalytic activities and were involved in metabolic, biological regulation and cellular processes. Besides, hypothetical protein and no match annotated results were found to fill a large part of those genes, indicating that effective approach should be adopted to discover novel genes in sugarcane genomics. Water stress resulted in an increase in ABA and IAA contents but a depression in GA content. Classified by biological process, 46 plant hormone related genes were selected, further annotation analysis showed that the metabolic pathways of some plant hormone responsive genes were diverse or had crosstalk with each other, indicating the intersectionality and complexity of plant hormone signaling pathway. Additionally, the relative expressions of nine selected genes were validated by quantitative real time PCR (qRT-PCR), further confirming the reliability of microarray results.

Key words: Sugarcane, Water stress, Microarray, Plant hormone, Gene expression profiles

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