Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (12): 1796-1805.doi: 10.3724/SP.J.1006.2019.91025

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Growth habit identification and diversity and stability analysis of heading date in Tibetan barley (Hordeum vulgare L.)

Lhundrupnamgyal1,2,3,4,5,Hui-Hui LI5,Gang-Gang GUO5, Chemiwangmo2,3,Li-Yun GAO2,3,Ya-Wei TANG2,3, Nyematashi2,4, Dawadondrup2,3,*(), Dolkar1,*()   

  1. 1 Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China
    2 State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa, Tibet 850002, China
    3 Institute of Agricultural Sciences, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa, Tibet 850002, China
    4 Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa, Tibet 850002, China
    5 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-03-20 Accepted:2019-06-22 Online:2019-12-12 Published:2019-07-15
  • Contact: Dawadondrup, Dolkar E-mail:dwdunzhu@126.com;slzhuoga@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31660299);the Tibet Department of Major Projects(XZ201801NA01);the Tibet Agricultural and Animal Husbandry College Graduate Innovation Program funded project YJS2017-07(502000114)

RichHTML359

34

PDF

703

Abstract:

To improve the utilization of Tibetan barley germplasm and select appropriate parents in hybridization breeding, evaluated the growth habit and heading date diversity of 1605 Tibetan barley germplasm under the spring and autumn sowing conditions in Nyingchi and Lhasa, Tibet. The growth habit was determined by the difference in heading date observed in two spring sowing dates in Lhasa, i.e. normal spring sowing I and 10 days later of spring sowing II, showing that 96.2% of the landraces were spring barley distributed in all three ecological regions; 3.8% of the landraces were the winter barley accessions originated mainly from the southeastern ecological region of Tibet. Shannon-Wiener’s analysis showed that the heading date diversity was higher under spring-sown environment than under autumn-sown environment. The heading date diversity was highest in Lhasa region with spring sowing, moderate in Nyingchi spring-sown environment, and lowest in Nyingchi autumn-sown environment. In the same environment, landraces had higher diversity than modern varieties. A total of 368 spring-type landraces and 21 varieties were stable in heading date diversity across environments, and the cultivar Kangqing 3 had the highest stability among all tested materials. This study helps to further understand the growth habits, heading dates diversity and environmental stability of barley resources in Tibet, and provides useful information for the development of broad-adapted barley varieties.

Key words: barley, growth habit, heading date diversity, stability

Fig. 1

Identification of spring/winter growth habit in Lhasa"

Table 1

Origin and type of Tibet barley germplasm"

来源
Origin		 类型
Type		 品种数
No. of accessions		 合计
Total		 比例
Proportion (%)
拉萨 Lhasa 春性地方品种 Spring landrace (SL) 125 155 9.64
冬性地方品种 Winter landrace (WL) 3
春性育成品种 Spring variety (SV) 21
冬性育成品种 Winter variety (WV) 6
山南 Lhoka 春性地方品种 Spring landrace (SL) 355 377 23.45
冬性地方品种 Winter landrace (WL) 12
春性育成品种 Spring variety (SV) 10
日喀则 Shigatse 春性地方品种 Spring landrace (SL) 538 573 35.63
冬性地方品种 Winter landrace (WL) 6
春性育成品种 Spring variety (SV) 29
昌都 Chamdo 春性地方品种 Spring landrace (SL) 275 282 17.60
冬性地方品种 Winter landrace (WL) 6
春性育成品种 Spring variety (SV) 1
林芝 Nyingchi 春性地方品种 Spring landrace (SL) 114 147 9.14
冬性地方品种 Winter landrace (WL) 30
春性育成品种 Spring variety (SV) 2
冬性育成品种 Winter variety (WV) 1
阿里 Ngari 春性地方品种 Spring landrace (SL) 40 40 2.49
那曲 Nagqu 春性地方品种 Spring landrace (SL) 1 1 0.06
西藏其他地区 Other Tibetan areas 春性育成品种 Spring variety (SV) 30 30 1.87

Fig. 2

Geographical distribution of different growth habit germplasm Gray line indicates the Yarlung Zangbo River basin, and the pie chart size represents the proportion to the entire material."

Fig. 3

Heading date distribution of Tibet barley germplasm in three environments Gray dotted line indicates the averaged value. SL: spring landrace; SV: spring variety; WL: winter landrace; WV: winter variety. 2017 Nyingchi-A: 2017 Nyingchi-Autumn sown; 2018 Nyingchi-S: 2018 Nyingchi-Spring sown; 2018 Lhasa-S: 2018 Lhasa-Spring sown."

Table 2

Changes in heading date and distribution characteristics of Tibet barley germplasm"

试验
Trial		 品种类型
Variety type		 品种数
No. of samples		 平均值 Mean±SD 变幅
Range		 变异系数CV (%) 多样性指数Shannon H°
2017年林芝秋播2017 Nyingchi-A 春性地方品种 Spring landrace (SL) 1332 174.54±9.33 160.00-204.00 5.53 1.33
冬性地方品种 Winter landrace (WL) 55 182.86±10.04 161.00-200.00 5.49 1.00
春性育成品种 Spring variety (SV) 93 174.40±6.68 160.00-202.00 3.83 1.13
冬性育成品种 Winter variety (WV) 7 185.33±8.36 180.00-202.00 4.51 0.56
2018年林芝春播2018 Nyingchi-S 春性地方品种 Spring landrace (SL) 1448 73.16±6.33 59.00-94.00 8.65 1.37
冬性地方品种 Winter landrace (WL) 52 84.72±6.39 64.00-97.00 7.55 1.25
春性育成品种 Spring variety (SV) 92 75.78±5.50 62.00-85.00 7.26 0.96
冬性育成品种 Winter variety (WV) 6 80.60±7.09 71.00-88.00 8.80 0.73
2018年拉萨春播2018 Lhasa-S 春性地方品种 Spring landrace (SL) 1448 68.19±5.62 53.00-85.00 8.36 1.41
冬性地方品种 Winter landrace (WL) 4 79.00±9.17 69.00-87.00 11.60 1.15
春性育成品种 Spring variety (SV) 93 70.18±4.19 59.00-81.00 6.04 0.64
冬性育成品种 Winter variety (WV) 4 73.75±3.28 71.00-75.00 4.45 0.69

Fig. 4

Comparison of heading date of different growth habit germplasm in three sowing times SL: spring landrace; SV: spring variety; WL: winter landrace; WV: winter variety. 2017 Nyingchi-A: 2017 Nyingchi-Autumn sown; 2018 Nyingchi-S: 2018 Nyingchi-Spring sown; 2018 Lhasa-S: 2018 Lhasa-Spring sown. Significant difference (P < 0.01)."

Fig. 5

Comparison of heading date between different environments under spring sowing conditions SL: spring landrace; SV: spring variety; WL: winter landrace; WV: winter variety. 2018 Nyingchi-S: 2018 Nyingchi-Spring sown; 2018 Lhasa-S: 2018 Lhasa-Spring sown. Significant difference (P<0.01)."

Fig. 6

Correlation between heading dates among different sowing time and environments A, B, C represents the correlation coefficient of sowing time or environments. 2017 Nyingchi-A: 2017 Nyingchi-Autumn sown; 2018 Nyingchi-S: 2018 Nyingchi-Spring sown; 2018 Lhasa-S: 2018 Lhasa-Spring sown."

Fig. 7

Frequency of five grades after standardization of heading date values in different sowing times I: early heading; II: partial early heading; III: middle heading; IV: partial late heading; V: late heading. 2017 Nyingchi-A: 2017 Nyingchi-Autumn sown; 2018 Nyingchi-S: 2018 Nyingchi-Spring sown; 2018 Lhasa-S: 2018 Lhasa-Spring sown."

Fig. 8

Stability analysis of different varieties in heading dates across different sowing times and environmental conditions SL: spring landrace; SV: spring variety; WL: winter landrace; WV: winter variety. I: early heading; II: partial early heading; III: middle heading; IV: partial late heading; V: late heading. a: 2017 Nyingchi-Autumn sown; b: 2018 Nyingchi-Spring sown; c: 2018 Lhasa-Spring sown. Red number indicates samples shared by the three environments."

Fig. 9

Stability analysis of the heading date of the main variety of Tibet barley in different sowing times and environments I: early heading; II: partial early heading; III: middle heading; IV: partial late heading; V: late heading. 2017 Nyingchi-A: 2017 Nyingchi-Autumn sown; 2018 Nyingchi-S: 2018 Nyingchi-Spring sown; 2018 Lhasa-S: 2018 Lhasa-Spring sown."

[1] Pourkheirandish M, Komatsuda T . The importance of barley genetics and domestication in a global perspective. Ann Bot, 2007,100:999-1008.
doi: 10.1093/aob/mcm139
[2] Turner A, Beales J, Faure S, Dunford R P, Laurie D A . The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley. Science, 2005,310:1031-1034.
doi: 10.1126/science.1117619
[3] Jones H, Leigh F J, Mackay I, Bower M A, Smith L M, Charles M P, Jones G, Jones M K, Brown T A, Powell W . Population based resequencing reveals that the flowering time adaptation of cultivated barley originated east of the Fertile Crescent. Mol Biol Evol, 2008,25:2211-2219.
doi: 10.1093/molbev/msn167
[4] Ibrahim A, Harrison M, Meinke H, Fan Y, Johnson P, Zhou M . A regulator of early flowering in barley (Hordeum vulgare L.). PLoS One, 2018,13:e0200722.
doi: 10.1371/journal.pone.0200722
[5] Doebley J F, Gaut B S, Smith B D . The molecular genetics of crop domestication. Cell, 2006,127:1309-1321.
doi: 10.1016/j.cell.2006.12.006
[6] Sang T . Genes and mutations underlying domestication transitions in grasses. Plant Physiol, 2009,149:63-70.
doi: 10.1104/pp.108.128827
[7] Trevaskis B, Bagnall D J, Ellis M H, Peacock W J, Dennis E S . MADS box genes control vernalization-induced flowering in cereals. Proc Natl Acad Sci USA, 2003,100:13099-13104.
doi: 10.1073/pnas.1635053100
[8] Saisho D, Ishii M, Hori K, Sato K . Natural variation of barley vernalization requirements: implication of quantitative variation of winter growth habit as an adaptive trait in East Asia. Plant Cell Physiol, 2011,52:775-784.
doi: 10.1093/pcp/pcr046
[9] Nishida H, Yoshida T, Kawakami K, Fujita M, Long B, Akashi Y, Laurie D A, Kato K . Structural variation in the 5′ upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat(Triticum aestivum L.), and their effect on heading time. Mol Breed, 2012,31:27-37.
doi: 10.1007/s11032-012-9765-0
[10] Comadran J, Kilian B, Russell J, Ramsay L, Stein N, Ganal M, Shaw P, Bayer M, Thomas W, Marshall D, Hedley P, Tondelli A, Pecchioni N, Francia E, Korzun V, Walther A, Waugh R . Natural variation in a homolog of Antirrhinum CENTRORADIALIS contributed to spring growth habit and environmental adaptation in cultivated barley. Nat Genet, 2012,44:1388-1392.
doi: 10.1038/ng.2447
[11] Laurie D A, Pratchett N, Bezant J H, Snape J W . RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum vulgare L.) cross. Genome, 1995,38:575-585.
doi: 10.1139/g95-074
[12] Yan L L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J . Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci USA, 2003,100:6263-6268.
doi: 10.1073/pnas.0937399100
[13] Yan L L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, Sanmiguel P, Bennetzen J L, Echenique V, Dubcovsky J . The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science, 2004,303:1640-1644.
doi: 10.1126/science.1094305
[14] Yan L L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J . The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci USA, 2006,103:19581-19586.
doi: 10.1073/pnas.0607142103
[15] Faure S, Higgins J, Turner A, Laurie D A . The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare). Genetics, 2007,176:599-609.
doi: 10.1534/genetics.106.069500
[16] Distelfeld A, Li C, Dubcovsky J . Regulation of flowering in temperate cereals. Curr Opin Plant Biol, 2009,12:178-184.
doi: 10.1016/j.pbi.2008.12.010
[17] 卢良恕 . 中国大麦学. 北京: 中国农业出版社, 1996.
Lu L S. Chinese Barley. Beijing: China Science and Technology Press, 1996. pp 3-7(in Chinese).
[18] 胡颂杰 . 西藏农业概论. 成都: 四川科学技术出版社, 1995.
Hu S J. An Introduction of Tibet Agriculture. Chengdu: Sichuan Science and Technology Press, 1995 ( in Chinese)
[19] Tashi N. Food preparation from hull-less barley in Tibet. In: Food Barley—Importance, Uses and Local Knowledge: Proc. International Workshop on Food Barley Improvement, 2002. Grando S, Macpherson H G, eds. ICARDA, Aleppo, Syria, 2005.
[20] 朱睦元, 张京 . 大麦(青稞)营养分析及其食品加工. 杭州: 浙江大学出版社, 2015.
Zhu M Y, Zhang J. Barley Nutritional Analysis and Food Processing. Hangzhou: Zhejiang University Press, 2015 (in Chinese).
[21] 马得泉 . 中国西藏大麦遗传资源. 北京: 中国农业出版社, 2000.
Ma D Q. Genetic Resources of Tibetan Barley in China. Beijing: China Science and Technology Press, 2000 (in Chinese).
[22] 张京, 曹永生 . 我国大麦基因库的群体结构和表型多样性研究. 中国农业科学, 1998,32(4):20-26.
Zhang J, Cao Y S . Population Structure and Phenotypic Diversity of Barley Gene Bank in China. Sci Agric Sin, 1998 (in Chinese with English abstract).
[23] 冯宗云 . 徐廷文大麦学术文集. 成都四川科学技术出版社, 2006.
Feng Z Y. Xu Tingwen's Work for Barley Science. Chengdu: Sichuan Science and Technology Press, 2006 ( in Chinese)
[24] 达瓦顿珠. 中国大麦低温春化和光周期基因单倍型及表型关联分析. 中国农业科学院研究生院博士学位论文, 北京, 2015. pp 25-28.
Dawadondrop. Haplotypes and Phenotypic Association Analsis of Vernalization and Photoperiod Genes in Chinese Barley in china. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2015. pp 25-28 (in Chinese with English abstract).
[25] Alqudah A M, Sharma R, Pasam R K, Graner A, Kilian B, Schnurbusch T . Genetic dissection of photoperiod response based on GWAS of pre-anthesis phase duration in spring barley. PLoS One, 2014,9:e113120.
doi: 10.1371/journal.pone.0113120
[26] Alqudah A M, Schnurbusch T . Heading date is not flowering time in spring barley. Front Plant Sci, 2017,8:896.
doi: 10.3389/fpls.2017.00896
[27] Hemming M N, Fieg S, Peacock W J, Dennis E S, Trevaskis B . Regions associated with repression of the barley (Hordeum vulgare) VERNALIZATION1 gene are not required for cold induction. Mol Genet Genom, 2009,282:107-117.
doi: 10.1007/s00438-009-0449-3
[28] Dondup D, Dong G Q, Xu D D, Zhang L S, Zha S, Yuan X M, Tashi N, Zhang J, Guo G G . Allelic variation and geographic distribution of vernalization genes HvVRN1 and HvVRN2 in Chinese barley germplasm. Mol Breed, 2016,36:11
doi: 10.1007/s11032-016-0434-6
[29] Shannon C E, Weaver W. The Mathematical Theory of Communication. Urbana: University of Illinons Press, 1949.
[30] 王建康 . 数量遗传学. 北京: 科学出版社, 2017.
Wang J K. Quantitative Genetics. Beijing: Science Press, 2017 (in Chinese).
[31] 强小林 . 西藏青稞品种系谱及其分析. 中国作物学会大麦专业委员会. 中国大麦文集. 北京: 中国农业科技出版社, 1986. pp 189-195.
Qiang X L. The pedigree and its analysis of barley varieties in Tibet. In: Barley Professional Committee of China Crop Society. Barley Science in China. Beijing: China Agricultural Science and Technology Press, 1986. pp 189-195(in Chinese).
[1] Niu Li, Wang Yong-Sheng, Wang Chang-Jie, Zhang Hong, Meng Ya-Xiong, Li Bao-Chun, Yang Ke, Ma Xiao-Le, Yao Li-Rong, Si Er-Jing, Wang Hua-Jun, Wang Jun-Cheng. Identification and analysis of the NAC gene family in barley (Hordeum vulgare L.) and functional validation of HvNAC38 in salt tolerance [J]. Acta Agronomica Sinica, 2026, 52(3): 688-707.
[2] Li Ying, Shi Xiao-Xu, Liu Hai-Cui, Shi Lyu, Xue Ya-Guang, Wei Ya-Feng. Establishment of a near-infrared reflectance spectroscopy model for predicting β-glucan content in naked barley grain [J]. Acta Agronomica Sinica, 2026, 52(3): 735-745.
[3] Yu Kai-Hang, Zhou Hong-Bin, Luo Liang-Zha, Wang Mei-Li, Jiang Rui-Mei, Dong-Chen Wen-Hua, Li Shi-Jin, Mao Xiao-Qiang, Chen Sheng-Wei. Cloning and expression analysis of the HvLRR-RLK-510 gene encoding a leucine-rich repeat receptor-like kinase in barley [J]. Acta Agronomica Sinica, 2026, 52(2): 421-432.
[4] HE Peng-Xu, YAO Li-Rong, CHEN Yuan-Ling, YAN Yan, ZHANG Hong, WANG Jun-Cheng, LI Bao-Chun, YANG Ke, SI Er-Jing, MENG Ya-Xiong, MA Xiao-Le, WANG Hua-Jun. Differences and correlations in physiological and molecular mechanisms of barley germination under drought stress [J]. Acta Agronomica Sinica, 2025, 51(9): 2412-2432.
[5] WEN Xuan, ZHONG Xiu-Li, WANG Shang-Wen, JIN Tao, PENG Jun, LIU En-Ke. Screening of low nitrogen tolerant germplasm in seedling highland barley based on tolerance index and comprehensive evaluation of different nitrogen efficiency types [J]. Acta Agronomica Sinica, 2025, 51(7): 1949-1958.
[6] ZOU Yi-Miao, YU Xiang-Ping, MIAO Yu-Cong, CAI Qian, DU Gui-Juan, ZHAO Feng-Yan, ZHANG Shi-Yu, LI Shuang-Yi, BAI Wei. Characteristics of soil organic carbon fraction accumulation and its stability in dry-crop farmland in northeast China after plough layer construction [J]. Acta Agronomica Sinica, 2025, 51(5): 1277-1285.
[7] LU Wen-Jia, WANG Jun-Cheng, YAO Li-Rong, ZHANG Hong, SI Er-Jing, YANG Ke, MENG Ya-Xiong, LI Bao-Chun, MA Xiao-Le, WANG Hua-Jun. Genome-wide identification of PRX gene family and analysis of their expressions under drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(5): 1198-1214.
[8] CHENG Hong-Na, QIN Dan-Dan, XU Fu-Chao, XU Qing, PENG Yan-Chun, SUN Long-Qing, XU Le, GUO Ying, YANG Xin-Quan, XU De-Ze, DONG Jing. Comparative analysis of metabolomics of colored hulless barley and colored wheat grains [J]. Acta Agronomica Sinica, 2025, 51(4): 932-942.
[9] WANG Lin, CHEN Xiao-Yu, ZHANG Wen-Meng-Long, WANG Si-Qi, CHENG Bing-Yun, CHENG Jing-Qiu, PAN Rui, ZHANG Wen-Ying. Molecular characteristics and functional analysis of HvMYB2 in response to drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(4): 873-887.
[10] WANG Chong-Ming, LU Zhi-Feng, YAN Jin-Yao, SONG Yi, WANG Kun-Kun, FANG Ya-Ting, LI Xiao-Kun, REN Tao, CONG Ri-Huan, LU Jian-Wei. Effect of phosphorus fertilizer rates on crop yield, phosphorus uptake and its stability in rapeseed-rice rotation system [J]. Acta Agronomica Sinica, 2025, 51(2): 447-458.
[11] WANG Yan-Ting, PANG Lei, ZHAO Jian-Hua, ZHENG Hao-Fei, MA Wen-Hao. Effects of different legume configurations with maize on yield stability of intercropping systems [J]. Acta Agronomica Sinica, 2025, 51(12): 3292-3303.
[12] LIU Jia-Hui, LI Yu-Long, WANG Ya-Ru, HE Hong, ZHANG Yun-Shu, WU Yu, ZENG Xiu-Li, LIU Ting-Hui, CHEN Guo-Yue, QI Peng-Fei, WEI Yu-Ming, JIANG Qian-Tao. Effects of natural variation in the SSIIa gene on starch composition and properties in Tibetan barley [J]. Acta Agronomica Sinica, 2025, 51(12): 3144-3156.
[13] HU Liang-Liang, ZHOU Hong-Mei, WANG Xiao-Lei, WANG Su-Hua, LI Cai-Ju, WEI Yun-Shan, WANG Li-Xia, CHENG Xu-Zhen, CHEN Hong-Lin. Analysis of genotype × environment interaction and stability of yield-related traits in adzuki bean (Vigna angularis) [J]. Acta Agronomica Sinica, 2025, 51(10): 2581-2594.
[14] MA Min-Hu, CHANG Hua-Yu, CHEN Zhao-Yan, REN Zeng, LIU Ting-Hui, XING Guo-Fang, GUO Gang-Gang. Identification and genome-wide association study of specialized seedling grass barley cultivars [J]. Acta Agronomica Sinica, 2025, 51(1): 91-102.
[15] SUN Man, AN Chao-Dan, GAO Guang-Qi, GUO Jie, YANG Ping, JIANG Cong-Cong. Genetic dissection of the albino hull mutations in barley (Hordeum vulgare L.) [J]. Acta Agronomica Sinica, 2024, 50(12): 3046-3054.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd