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作物学报 ›› 2014, Vol. 40 ›› Issue (03): 550-555.doi: 10.3724/SP.J.1006.2014.00550
叶艳英1.2,曾钢1,曹鸣庆1,马荣才1,吴才君2,*,姚磊1,*
YE Yan-Ying1.2,ZENG Gang1,CAO Ming-Qing1,MA Rong-Cai1,WU Cai-Jun2,*,YAO Lei1,*
摘要：
芜菁花叶病毒(turnip mosaic viruses,TuMV)是侵染重要经济作物的主要病毒之一，寄主范围十分广泛，尤其是对十字花科作物的危害最为严重。为了获得对TuMV持久稳定的高度抗性，本研究以TuMV HC-Pro基因的453 bp保守序列为靶标，构建了植物表达RNAi载体pBBBTu-HC-Pro，并转化了TuMV的天然宿主拟南芥。对转基因拟南芥用北京地区流行的TuMV强致病株BJ-C4进行了抗病接种鉴定。鉴定的13个单拷贝转基因株系中有4个对TuMV的BJ-C4株表现出高度抗性，抗性比对照提高约80%以上，且抗性可以稳定遗传。经半定量和荧光定量PCR方法检测，在高抗转基因植株体内几乎检测不到病毒的累积，抗病效果明显。该载体在利用基因工程抗TuMV育种中具有广阔的应用前景。
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