作物学报 ›› 2014, Vol. 40 ›› Issue (04): 745-750.doi: 10.3724/SP.J.1006.2014.00745
王淑文,陆俊杏,万华方,翁昌梅,王珍,李加纳,卢坤,梁颖*
WANG Shu-Wen,LU Jun-Xing,WAN Hua-Fang,WENG Chang-Mei,WANG Zhen,LI Jia-Na,LU KunLIANG Ying*
摘要:
植物MAPKs (mitogen-activated protein kinases)在多种生物和非生物胁迫中起重要作用。课题组前期克隆了甘蓝型油菜BnMAPK1基因,并获得BnMAPK1超量表达植株。本文以甘蓝型油菜中油821DH系为对照,以超量表达BnMAPK1的转基因油菜为试材,采用离体叶片接种的方法,测定染病叶片病斑的大小及病斑周围叶片的草酸含量,并用qRT-PCR检测转基因植株4个病程相关蛋白(OXO、Cu/ZnSOD、PR2、PR3)编码基因在核盘菌胁迫下的相对表达动态变化。结果表明,甘蓝型油菜BnMAPK1超量表达可显著抑制核盘菌对离体叶片的侵染,控制染病叶片内草酸毒素的积累,可能可以解除核盘菌对OXO表达的负调控,使另外3个病程相关蛋白基因(Cu/ZnSOD、PR2、PR3)表达上调。表明BnMAPK1超量表达可有效提高油菜菌核病抗性。
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