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作物学报 ›› 2024, Vol. 50 ›› Issue (7): 1699-1709.doi: 10.3724/SP.J.1006.2024.34198

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆种质资源萌发期耐莠去津鉴定评价及优异种质筛选

李晓菲1,2,**(), 高华伟2,3,**, 广慧2,4,5, 石宇欣1,2, 谷勇哲2, 齐照明1,*(), 邱丽娟1,2,*()   

  1. 1东北农业大学农学院, 黑龙江哈尔滨 150030
    2农作物基因资源与遗传改良国家重大科学工程 / 农业农村部种质资源利用重点实验室 / 中国农业科学院作物科学研究所, 北京 100081
    3三亚中国农业科学院国家南繁研究院, 海南三亚 572024
    4黑龙江护理高等专科学校, 黑龙江哈尔滨 150028
    5哈尔滨师范大学生命科学与技术学院, 黑龙江哈尔滨 150025
  • 收稿日期:2023-11-23 接受日期:2024-01-30 出版日期:2024-07-12 网络出版日期:2024-02-20
  • 通讯作者: *齐照明, E-mail: qizhaoming1860@126.com;邱丽娟, E-mail: qiulijuan@caas.cn
  • 作者简介:李晓菲, E-mail: 1612025234@qq.com

    **同等贡献

  • 基金资助:
    海南省重点研发计划项目(ZDYF2021XDNY275)

Identification and evaluation of atrazine tolerance of soybean germplasm resources at germination stage and screening of excellent germplasm

LI Xiao-Fei1,2,**(), GAO Hua-Wei2,3,**, GUANG Hui2,4,5, SHI Yu-Xin1,2, GU Yong-Zhe2, QI Zhao-Ming1,*(), QIU Li-Juan1,2,*()   

  1. 1College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
    2National Key Facility for Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture and Rural Affairs / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, Hainan, China
    4Heilongjiang Nursing College, Harbin 150028, Heilongjiang, China
    5College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, Heilongjiang, China
  • Received:2023-11-23 Accepted:2024-01-30 Published:2024-07-12 Published online:2024-02-20
  • Contact: *E-mail: qizhaoming1860@126.com; E-mail: qiulijuan@caas.cn
  • About author:

    **Contributed equally to this work

  • Supported by:
    Key Research and Development Program of Hainan Province(ZDYF2021XDNY275)

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摘要：

室内生物测定是农作物除草剂耐受性鉴定的常用方法, 已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究, 但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道。本研究以莠去津对大豆萌发期各单项指标为评价指标, 分析莠去津胁迫条件下大豆发芽势、发芽率、发芽指数、活力指数、根长等性状的变化, 建立大豆莠去津耐受性室内生测方法, 确定3.5 mL L-1是大豆萌发期莠去津耐受性筛选的适宜浓度; 对159份大豆种质进行萌发期莠去津耐受性鉴定表明, 各指标相对值的变异系数为发芽相对莠去津胁迫率>相对芽长>相对发芽指数>相对活力指数>相对发芽势>相对发芽率; 根据T值的聚类分析, 将供试种质分为4个莠去津耐性级别, 共鉴定出高耐种质9份、中耐种质50份、低耐种质70份和敏感种质30份; 黄豆、花色豆、垦丰13、南农99-6等9个种质为莠去津耐受性综合能力优异的种质资源。采用多元逐步回归分析法, 建立大豆萌发期耐莠去津评价模型Y = -0.155+0.004X2+0.001X3+0.001X4+0.002X5 (P=0.000)。本研究为培育耐莠去津育种的亲本选配、后代选择及大豆耐莠去津相关基因挖掘提供了理论依据、材料和技术支撑。

关键词: 大豆, 种质资源, 莠去津耐性, 萌发期, 综合评价

Abstract:

Laboratory bioassay is a common method to identify crop herbicide tolerance, which was widely used in soybean, cotton, and other crops to study the tolerance of glyphosate, chlorsulfuron and other herbicides, but there are few reports on laboratory bioassay method and soybean tolerance to atrazine. In this study, the effects of atrazine on the germination stage of soybean were evaluated, and the changes of germination potential, germination rate, germination index, vitality index, and root length of soybean under atrazine stress were analyzed. A laboratory bioassay method was established for the atrazine tolerance of soybean, and 3.5 mL L-1 was determined as the appropriate concentration for screening atrazine tolerance of soybean at germination stage. The tolerance of atrazine to 159 soybean germplasms at germination stage was identified. The results showed that the relative coefficient of variation of each index was the relative atrazine stress rate, the relative bud length, the relative germination index, the relative vitality index, the relative germination potential, and the relative germination rate. According to the T-value cluster analysis, the tested germplasm was divided into 4 atrazine tolerance grades, and 9 high-tolerant, 50 medium-tolerant, 70 low-tolerant, and 30 sensitive germplasm were identified. Nine germplasm, including soybean, fancy bean, Kenfeng 13, and Nannong 99-6, had excellent tolerance to atrazine. This study provides the theoretical basis, material and technical support for the selection of parents, the selection of progeny and the mining of genes related to atrazine tolerance in soybean.

Key words: soybean, germplasm resources, atrazine tolerance, germination stage, comprehensive evaluation

附表1

供试材料"

序号
Number		 统一编号
Unified number		 序号
Number		 统一编号
Unified number		 序号
Number		 统一编号
Unified number		 序号
Number		 统一编号
Unified number
1 WDD00329 41 ZDD24640 81 ZDD03257 121 ZDD12325
2 WDD00596 42 ZDD25261 82 ZDD03281 122 ZDD12403
3 WDD01511 43 华夏10 Huaxia 10 83 ZDD03289 123 ZDD12845
4 WDD01944 44 华夏14 Huaxia 14 84 ZDD03481 124 WDD03020
5 WDD01945 45 ZLD1501 85 ZDD03555 125 ZDD15708
6 WDD02225 46 ZLD1510 86 ZDD03663 126 WDD01607
7 ZDD00383 47 ZLD2109 87 ZDD03901 127 ZDD17283
8 ZDD01400 48 ZDD22643 88 WDD01594 128 ZDD20127
9 ZDD01881 49 ZDD24374 89 ZDD09985 129 ZDD20186
10 ZDD02990 50 WDD00573 90 ZDD10039 130 ZDD20332
11 ZDD03138 51 ZDD00196 91 ZDD18642 131 WDD01665
12 ZDD03533 52 ZDD00296 92 ZDD18657 132 WDD01650
13 ZDD05451 53 ZDD00303 93 ZDD19107 133 ZDD22123
14 ZDD05514 54 ZDD00485 94 ZDD19410 134 ZDD06290
15 ZDD06270 55 ZDD00554 95 ZDD04395 135 ZDD14472
16 ZDD06354 56 ZDD00978 96 ZDD06514 136 ZDD14780
17 ZDD06418 57 ZDD01010 97 ZDD12436 137 ZDD21387
18 ZDD08981 58 ZDD07186 98 ZDD12500 138 ZDD01421
19 ZDD09417 59 ZDD07191 99 ZDD13117 139 ZDD03026
20 ZDD10060 60 ZDD07307 100 ZDD20005 140 ZDD03293
21 ZDD10080 61 ZDD07353 101 ZDD20886 141 ZDD08146
22 ZDD11575 62 ZDD07685 102 ZDD21030 142 ZDD00772
23 ZDD12801 63 ZDD17728 103 ZDD21053 143 ZDD01612
24 ZDD15031 64 ZDD17882 104 ZDD21852 144 ZDD03428
25 ZDD16869 65 ZDD18378 105 ZDD06595 145 ZDD00076
26 ZDD16872 66 ZDD22640 106 ZDD06669 146 ZDD03733
27 ZDD18856 67 ZDD23645 107 ZDD14182 147 ZDD04429
28 ZDD19368 68 ZDD23650 108 ZDD15213 148 ZDD16682
29 ZDD19804 69 ZDD24317 109 ZDD16700 149 ZDD23915
30 ZDD20303 70 ZDD24326 110 ZDD16914 150 ZDD23089
31 ZDD21781 71 WDD01630 111 ZDD18394 151 ZDD11465
32 ZDD21920 72 ZDD01637 112 ZDD25258 152 ZDD16362
33 ZDD24145 73 ZDD01668 113 ZDD04653 153 ZDD08033
34 ZDD24342 74 ZDD02277 114 ZDD04864 154 WDD02262
35 ZDD24801 75 WDD02183 115 ZDD05525 155 WDD00602
36 黑河43 Heihe 43 76 ZDD18847 116 ZDD05879 156 WDD00640
37 黑农84 Heinong 84 77 WDD00412 117 ZDD05921 157 WDD01664
38 齐小粒Qixiaoli 78 ZDD23891 118 ZDD06039 158 WDD00853
39 中吉602 Zhongji 602 79 ZDD24065 119 ZDD06066 159 WDD02282
40 齐黄34 Qihuang 34 80 ZDD02826 120 ZDD12017

图1

大豆对不同浓度莠去津的耐受性"

表1

不同浓度莠去津处理下4个大豆种质的发芽指数"

材料
Material		 发芽指数Germination index
0 mL L-1 1 mL L-1 2 mL L-1 2.5 mL L-1 3 mL L-1 3.5 mL L-1 4 mL L-1 4.5 mL L-1 5 mL L-1
MY526 8.14±0.97 a 7.00±0.73 ab 6.47±1.46 ab 6.33±1.17 ab 5.17±1.61 ab 4.39±1.26 b 5.11±0.19 ab 4.58±0.8 b 4.5±0.6 b
中黄13 Zhonghuang 13 4.11 ±0.67 a 3.56±0.98 ab 3.42±0.74 ab 4.00±0.44 ab 3.75±0.58 ab 1.86 ±0.05 b 2.19±0.17 ab 1.89±0.69 ab 1.83±0.04 b
ZLD1505 8.61±0.67 a 8.33 ±0.58 ab 7.72 ±0.35 abc 8.22±0.84 ab 7.61±0.67 abc 7.17±0.6 abc 6.89±1.39 abc 6.39±0.92 bc 5.94 ±0.42 c
中豆41 Zhongdou 41 4.67 ±1.3 a 5.61±0.99 a 5.17 ±0.83 a 4.39 ±2.36 a 4.33±0.67 a 3.75±1.04 a 3.78±0.48 a 3.61±1.46 a 3.47 ±1.28 a

表2

不同浓度莠去津处理下4个大豆种质的活力指数"

材料
Material		 活力指数Vitality index
0 mL L-1 1 mL L-1 2 mL L-1 2.5 mL L-1 3 mL L-1 3.5 mL L-1 4 mL L-1 4.5 mL L-1 5 mL L-1
MY526 406.20±7.56 a 239.63±6.15 ab 204.45±3.86 ab 196.59 ±11.3 ab 166.9±13.76 b 98.23±4.61 b 135.93±3.2 b 111.47±5.14 b 111.90±4.5 b
中黄13 Zhonghuang 13 102.74±1.77 a 70.64 ±3.82 ab 60.55±7.97 ab 87.42 ±6.43 ab 69.62±3.01 ab 15.95±2.5 b 34.24±2.06 ab 23.19±2.87 b 35.67±6.25 ab
ZLD1505 459.79±6.77 a 361.93±7.6 abc 348.83±6.08 abc 403.04 ±4.39 ab 338.09 ±8.26 abc 289.33±6.93 bc 272.62 ±2/03 bc 239.14 ±2.78 bc 201.47±5.44 c
中豆41 Zhongdou 41 162.20±8.84 a 217.41±7.79 a 194.79 ±13.24 a 163.23±18.86 a 169.36±9.71 a 119.44±6.08 a 96.83±3.32 a 81.00±5.01 a 118.57 ±12.22 a

表3

3.5 mL L-1的莠去津胁迫下大豆种质萌发期各指标的相对值(部分显示)"

编号Number 材料
Material		 相对发芽势
Relative germination potential		 相对
发芽率
Relative germination rate		 相对
发芽指数
Relative germination index		 相对根长
Relative root length		 相对活力
指数
Relative vitality index		 发芽相对
胁迫率
Relative stress rate of germination
35 南农99-6 Nannong 99-6 108.84 119.89 92.91 124.05 119.33 -19.89
51 秃荚子Tujiazi 135.00 138.33 162.91 56.43 126.69 -38.33
56 铁荚子Tiejiazi 113.89 113.89 114.29 66.67 18.65 -13.89
60 九台薄地高Jiutaibodigao 62.38 49.54 57.29 78.66 45.66 50.46
61 汪清神仙洞Wangqingshenxiandong 51.59 47.98 47.80 121.65 84.69 52.02
78 中豆27 Zhongdou 27 100.00 100.00 102.35 102.68 106.78 0
96 湘豆3号Xiangdou 3 113.69 113.69 167.92 113.98 191.10 -13.69
129 八月黄Bayuehuang 48.62 55.29 60.77 48.00 32.10 44.71
149 五星1号 Wuxing 1 106.61 116.93 113.55 82.59 96.08 -16.93
159 丹丽Danli 56.55 56.55 51.43 48.61 26.66 43.45
最大值Max. 135.00 138.33 203.11 132.07 191.10 52.02
最小值Min. 48.62 47.98 47.08 10.75 5.87 -38.33
平均值Mean 89.51 90.12 87.31 78.15 70.84 9.91
标准差Standard deviation 14.46 14.60 22.29 19.19 29.05 14.61
变异系数Coefficient of variation (%) 16.15 16.20 25.53 24.55 41.01 147.38

图2

3.5 mL L-1的莠去津胁迫下大豆各指标相对值的相关分析 对角线位置的柱状图表示每个性状的分布情况; 对角线的左下角表示性状之间的相关散点图, 对角线右上角的数量表示对应性状之间的参数统计, 同时红线表示相关的趋势。***表示在0.001概率水平显著相关。"

附表2

莠去津胁迫下大豆萌发期各指标的主成分及贡献率"

主成分
Principal component		 特征值
Eigenvalue		 贡献率
Contribution rate		 累计贡献率
Cumulative contribution rate
1 4.189 69.817 69.817
2 1.155 19.251 89.068
3 0.412 6.866 95.934
4 0.180 2.994 98.928
5 0.038 0.636 99.564
6 0.026 0.436 100.000

表4

莠去津胁迫条件下大豆萌发期各指标的因子载荷矩阵"

指标Index 主成分1 Principal component 1 主成分2 Principal component 2
相对发芽势Relative germination potential 0.221 -0.219
相对发芽率Relative germination rate 0.223 -0.242
相对发芽指数Relative germination index 0.210 -0.043
相对活力指数Relative vitality index 0.123 0.702
相对根长Relative root length 0.201 0.420
发芽相对莠去津指数Germination relative to atrazine index -0.201 0.296

表5

高耐种质的综合指标值(Kj)、权重、隶属函数值R(Kj)、T值及综合评价"

序号Number 统一编号
Unified
number		 材料
Material		 K1 K2 R(K1) R(K2) T值
T-value		 耐药性
Herbicide resistance
18 ZDD08981 黄豆Huangdou 1.744 1.085 0.735 0.648 0.716 高耐High tolerance
22 ZDD11575 花色豆Huasedou 1.865 1.253 0.753 0.670 0.735 高耐High tolerance
34 ZDD24342 垦丰13 Kenfeng 13 1.413 1.502 0.685 0.702 0.689 高耐High tolerance
35 ZDD24801 南农99-6 Nannong 99-6 1.761 1.116 0.737 0.652 0.719 高耐High tolerance
50 ZDD00303 秃荚子Tujiazi 2.916 -2.385 0.910 0.197 0.756 高耐High tolerance
51 ZDD00296 宝青绿大豆Baolvqingdadou 2.324 1.551 0.821 0.708 0.797 高耐High tolerance
57 ZDD07186 宾县黑豆Binxianheidou 1.045 2.874 0.630 0.880 0.684 高耐High tolerance
89 ZDD10039 爬蔓黑豆Pamanheidou 3.519 -0.103 1.000 0.493 0.891 高耐High tolerance
95 ZDD06514 湘豆3号Xiangdou 3 2.815 1.768 0.895 0.737 0.861 高耐High tolerance
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