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作物学报 ›› 2025, Vol. 51 ›› Issue (10): 2595-2604.doi: 10.3724/SP.J.1006.2025.53023

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

ZmMS13不同突变产生了gms1和yems1166复等位玉米雄性不育系

赵志文(), 陈慧(), 连玉杰, 陆涵, 曹旭东, 王帆, 喻梦璠, 张战辉, 汤继华, 陈晓阳()   

  1. 河南农业大学农学院 / 小麦玉米两熟高效生产全国重点实验室, 河南郑州 450046
  • 收稿日期:2025-04-15 接受日期:2025-07-09 出版日期:2025-10-12 网络出版日期:2025-07-15
  • 通讯作者: *陈晓阳, E-mail: cxy759020@henau.edu.cn
  • 作者简介:赵志文, E-mail: zzw16692520062@163.com;
    陈慧, E-mail: 17339301889@163.com
    **同等贡献
  • 基金资助:
    河南省科技研发计划联合基金(优势学科培育类)项目(242301420131);郑州市“揭榜挂帅”制重点研发专项(2023JBGS013)

Different mutations in ZmMS13 generate the gms1 and yems1166 multiple allele maize male-sterile lines

ZHAO Zhi-Wen(), CHEN Hui(), LIAN Yu-Jie, LU Han, CAO Xu-Dong, WANG Fan, YU Meng-Fan, ZHANG Zhan-Hui, TANG Ji-Hua, CHEN Xiao-Yang()   

  1. College of Agronomy, Henan Agricultural University / State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Zhengzhou 450046, Henan, China
  • Received:2025-04-15 Accepted:2025-07-09 Published:2025-10-12 Published online:2025-07-15
  • Contact: *E-mail: cxy759020@henau.edu.cn
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    Joint Foundation for Science and Technology Research and Development Program of Henan Province (Cultivation of Superior Disciplines)(242301420131);Zhengzhou Research and Development Project of Open Bidding for Selecting the Best Candidates(2023JBGS013)

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摘要：

玉米雄性不育系的创制与应用是降低玉米杂交种制种成本、提高种子纯度的有效途径。本研究鉴定到2个玉米雄性核不育突变体gms1和yems1166, 表型分析表明突变体花药角质层结构异常, 小孢子在单核后期开始降解, 成熟期突变体无花粉粒形成。遗传分析表明2个突变体不育性状受细胞核隐性单基因控制。通过图位克隆的策略将GMS1和YEMS1166基因分别定位于第5染色体23.52~26.09 Mb和24.86~30.95 Mb区间, 该区间包含已报道的脂类转运蛋白基因ZmMS13, 编码ABCG转运蛋白ZmABCG2a。基因测序结果显示gms1在ZmMS13基因第5外显子上有1个碱基的替换(TCA>TGA), 导致其翻译提前终止; 突变体yems1166在ZmMS13基因的第1外显子上有8个碱基的缺失, 导致其翻译提前终止。等位性测验进一步证明gms1和yems1166为ZmMS13的新等位突变体。本研究鉴定到2个新的ZmMS13基因等位突变体, 为玉米雄性不育化杂交制种提供了新的种质资源。

关键词: 玉米, 雄性不育, 角质层, ms13, ABCG转运蛋白

Abstract:

The development and utilization of maize male-sterile lines is an effective strategy to reduce the cost of hybrid seed production and improve seed purity. In this study, two maize male-sterile mutants, gms1 and yems1166, were identified. Phenotypic analysis revealed that both mutants exhibited abnormal anther cuticle structures, microspore degradation at the late uninucleate stage, and complete absence of mature pollen grains. Genetic analysis indicated that male sterility in both mutants is controlled by a single recessive nuclear gene. Using a positional cloning approach, the GMS1 and YEMS1166 loci were mapped to intervals of 23.52-26.09 Mb and 24.86-30.95 Mb on chromosome 5, respectively, regions containing the previously reported male fertility gene ZmMS13, which encodes the ABCG transporter ZmABCG2a. Gene sequencing revealed that gms1 carries a single base substitution (TCA>TGA) in the fifth exon of ZmMS13, resulting in a premature stop codon. The yems1166 mutant harbors an 8-bp deletion in the first exon of the same gene, also leading to a premature stop codon. Allelic tests confirmed that gms1 and yems1166 are novel allelic variants of ZmMS13. These findings identify two new allelic mutants of ZmMS13 and provide valuable germplasm resources for the development of male-sterile lines in maize hybrid seed production.

Key words: maize, male sterility, anther cuticle, ms13, ABCG transporter

表1

基因定位的引物序列"

引物名称
Primer name		 正向引物序列
Forward primer sequence (5′-3′)		 反向引物序列
Reverse primer sequence (5′-3′)
S08836 ACCAGTCCGCCACTACTATTCG CCGCTCCATCCGTCCTGTC
S08958 GGTGCCCGTTACATCAGATCAG GTGTGACCGTGTGAGTGGAATC
S08994 TGCTTGCCTTCTCCTGTTTGAC GCTCGGTTCACGCCTTCAC
S09011 TCCGTTCGGAATCACTTGTTGG TTCAGGAGGGAGCGGAGTTG
S09069 GCAACGTGCATGTGTGTG GTCAACGGCCAGCCCTAC
S09079 GCCAGCGTCACCGTCGTC TGTCGTGCGGAAGCCTGAAG
S09092 TCCTTATCCCTGCCAAGAAACG AGAGTGCGGAGAGTGAGACC
S09100 CCTTTCTGAATGGAGGGAGTAC CGTCTGGCTTCTTTTCATGTTG
S09128 TTAGGATCAGCAAGGCATAGGG GAGCGTTGTCAGGTAAGTTTGG
S09130 TGACTCATTCAGCAGCCTGGTG CCGTTGACTGGTCCACCTGTAC
S09138 TACCCGTCCCAATTCCCAACC AAGCAGCGAACCAGCACTTG
S09144 CCTTGGCTGTCTCCAGTAGTCC CCTGCTGGTCCTCTGGTTGTG
S09146 TGCCTGCCTATATCTTCGACAC TCAATTCCTCGGTAGCGTCATC
S09154 GCTTCTTGTCGGAAATCGTCAC TTTGGCAGGTTCAGCTAATTGC
S09160 GACACCATGCCTGTGACTGAG AAAGCCGTTTCTTGCCCTCTG
S09172 GCCGTGTTGGAATGATGGTC TCTTCAGCTAGAGAGGTGGTAC
S09220 CAACAACACAACGCCACAAC TGATGCCATGATGACCTGTATG

图1

开花期野生型和突变体gms1表型分析 A: 野生型和gms1植株, 标尺=30 cm; B: 野生型和gms1雄穗, 标尺=1 cm; C: 野生型和gms1小穗, 标尺=2 mm; D: 野生型花粉粒, 标尺=100 μm; E: gms1花粉粒, 标尺=100 μm。"

图2

不同发育时期野生型和突变体gms1花药形态比较 S8b: 四分体时期; S9: 单核小孢子前期; S10: 单核小孢子后期; S11: 双核时期; S13: 成熟期。标尺=1 mm。"

图3

不同发育时期小孢子发育观察 A, B: 四分体时期; C, D: 单核小孢子前期; E, F: 单核小孢子后期; H, I: 双核时期; J, K: 成熟期。WT: 野生型。标尺=100 μm。"

图4

成熟期花药角质层结构分析 A, B: 花药, 标尺=1 mm; C, D: 花药外表面, 标尺=25 μm; E, F: 花药内表面, WT: 野生型。标尺=5 μm。"

表2

F2分离群体雄性可育株与不育株分离比"

时间
Time		 地点
Location		 F2分离群体
F2 population		 雄性可育株
Male fertile plants		 雄性不育株
Male sterile plants		 χ2
2022年春Spring in 2022 原阳Yuanyang gms1×B73 108 26 2.328
2022年夏Summer in 2022 原阳Yuanyang gms1×B73 46 15 0.011

图5

GMS1基因定位"

图6

YEMS1166基因定位"

表3

群体雄性可育株与不育株分离比"

时间
Time		 地点
Location		 群体
Population		 雄性可育株
Male fertile plants		 雄性不育株
Male sterile plants		 χ2
2023 海南Hainan gms1×(yems1166×B73) 31 28 0.508
yems1166×(gms1×B73) 37 38 0.027
2023 原阳Yuanyang gms1×(yems1166×B73) 35 32 0.400
yems1166 ×(gms1×B73) 27 24 0.467

图7

ZmMS13基因测序"

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