欢迎访问作物学报，今天是
作物学报 ›› 2024, Vol. 50 ›› Issue (9): 2219-2236.doi: 10.3724/SP.J.1006.2024.34214
杨煜琛2(
), 靳雅荣2, 骆金婵2, 祝鑫2, 李葳航2, 贾纪原2, 王小珊2, 黄德均1,*(), 黄琳凯2,*()
YANG Yu-Chen2(), JIN Ya-Rong2, LUO Jin-Chan2, ZHU Xin2, LI Wei-Hang2, JIA Ji-Yuan2, WANG Xiao-Shan2, HUANG De-Jun1,*(), HUANG Lin-Kai2,*()
摘要：
珍珠粟是世界范围重要的谷物, 拥有极强的光合能力和高生产潜力, 较其他作物相比, 珍珠粟具有对贫瘠土壤的耐受性, 能够适应多种非生物胁迫和多样化的环境条件。PgWD40基因家族在植物抵御生物和非生物胁迫以及调控植物生长发育中扮演着重要角色。本研究利用生物信息学方法全面鉴定和分析了珍珠粟中的PgWD40基因家族及其表达模式。研究结果显示, 共鉴定出209个PgWD40基因家族成员, 通过对珍珠粟和水稻的系统进化分析将其归为5个亚家族, 在同一亚族内的成员中, 它们的保守序列和基因结构表现出一定的相似性。此外, 通过对启动子顺式作用元件的分析显示, 176个PgWD40基因与植物的生长和发育相关, 208个PgWD40基因成员含有不同激素胁迫响应的顺式作用元件, 进一步通过转录组数据分析和qRT-PCR分析显示, PMA3G03393.1、PMA4G00558.1、PMA5G02217.1等基因受到盐、热和干旱胁迫的诱导, 表明这些基因可能通过依赖不同激素的信号通路来调控和响应非生物胁迫, 可作为进一步研究PgWD40基因家族耐受性功能的候选基因。并且PgWD40基因家族成员在珍珠粟抽穗期的不同时期存在表达差异。通过基因表达热图以及GO和KEGG等分析, 发现许多PgWD40基因家族成员参与了植物生长发育和籽粒形成的各个阶段。研究结果为全面解析PgWD40基因结构与生物学功能、耐逆性分子机制以及分子育种提供了理论基础, 为今后培育高效抗逆作物新品种提供基因资源。
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