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作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1344-1349.doi: 10.3724/SP.J.1006.2009.01344

• 耕作栽培·生理生化 • 上一篇    下一篇

缺磷对已结瘤大豆生长和固氮功能的影响

苗淑杰，乔云发，韩晓增*，王树起，李海波   

  1. 中国科学院东北地理与农业生态研究所，黑龙江哈尔滨150081
  • 收稿日期:2008-12-20 修回日期:2009-03-18 出版日期:2009-07-12 网络出版日期:2009-05-19
  • 作者简介:E-mail: miaoshujie@126.com; Tel: 0451-86648128
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2005CB121101）和中国科学院东北地理与农业生态研究所青年博士基金资助。

Effects of Phosphorus Deficiency on Growth and Nitrogen Fixation of Soybean after Nodule Formation

MIAO Shu-Jie,QIAO Yun-Fa,HAN Xiao-Zeng*,WANG Shu-Qi,LI Hai-Bo   

  1. Northeast Institute of Geography and Agro-exology,Chinese Academy of Sciences,Harbin 150081,China
  • Received:2008-12-20 Revised:2009-03-18 Published:2009-07-12 Published online:2009-05-19
  • About author:E-mail: miaoshujie@126.com; Tel: 0451-86648128

PDF

2230

被引次数

40

摘要：

磷对大豆生长起着非常重要的作用，然而区别磷在大豆生长和根瘤功能中的影响非常困难。为阐明根瘤形成后缺磷对大豆植株生长和固氮功能的影响，采用水培方法，营养液中供给正常磷酸盐浓度(30 µmol L-1)条件下，待根瘤形成后，将营养液中的磷水平分别转换为0 µmol L-1 (无磷)和4 µmol L-1 (缺磷)，研究缺磷对大豆生长和结瘤固氮的影响。结果表明，大豆根瘤具有固氮功能后，进行营养液中不同浓度的磷酸盐处理，从处理第9天开始，缺磷对大豆生物量的影响才表现出来，而缺磷对根瘤形成和生长的影响从处理开始就比较明显。处理第9天，无磷、缺磷和正常磷处理根瘤数比处理第3天分别增加11.8%、15.4%和20.0%，单位植株豆血红蛋白含量和单位根瘤豆血红蛋白含量都随磷浓度增加而增加。该结果表明，在保证大豆形成一定数目的根瘤后，缺磷会明显影响根瘤生长和固氮能力。

关键词: 大豆, 根瘤, 固氮

Abstract:

Phosphorus had a key role in soybean growth, however, it is very different to separate the role in soybean growth from that in nodule function. The research was conducted to elucidate the effects of phosphorus deficiency on growth and nitrogen fixation after nodule formation in soybean. Soybean seedlings were cultivated in nutrient solution culture with normal phosphorus concentration (30 µmol L-1), which was shifted to 0 and 4 µmol L-1 after nodule formation resulting in phosphorus deficienct for soybean plants.The results showed that phosphorus deficiency negatively influenced soybean plant growth at the ninth day of phosphorus deficiency treatment and nitrogen fixation at the beginning of the treatnent. The nodule number increased by 11.8%, 15.4%, and 20.0%, respectively in 0, 4, and 30 µmol L-1 phosphorus treatments at the ninth of treatment (DOT) compared with that at the third DOT. Leghemoglobin concentration per gram of plant and per nodule biomass increased with phosphorus concentration increase. These suggested that phosphorus deficiency affected significantly nodule development and nitrogen fixation when soybean plants formed sufficient nodule.

Key words: Soybean, Phosphorus, Nodule, Nitrogen fixation

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