作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1409-1413.doi: 10.3724/SP.J.1006.2010.01409
刘珊珊,滕卫丽**,张彬彬,葛玉君,刁桂珠,郑天慧,姜自芹,曾蕊,吴帅,李文滨*
LIU Shan-Shan,TENG Wei-Li**,ZHANG Bin-Bin,GE Yu-Jun,DIAO Gui-Zhu,ZHENG Tian-Hui,JIANG Zi-Qin,ZENG Rui,WU Shuai,LI Wen-Bin*
摘要:
采用常规杂交育种方法,人工去雄、授粉,以10份综合农艺性状优良的黑龙江省主栽品种(或优良品系)为母本、亚基组成为(α'+α+11S酸性亚基)-缺失型育种材料日B为父本进行有性杂交。将F1杂交种南繁,单粒点播、单株收获得到F2代分离群体。聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析表明,F1代杂交种子7S球蛋白α'-与α-亚基的表现型全部为正常型。在杂交F2代种子中获得了具有中国大豆遗传背景的α-缺失、(α+A1aA1bA2)-缺失、A3-缺失、(α'+A4)-缺失和(α '+α)-缺失的贮藏蛋白亚基组成新类型种质, 为我国大豆蛋白组分育种选择提供了重要的中间材料。
| [1]Fukushina D. Recent progress of soybean protein foods. Food Rev Intl, 1991, (7): 323-325[2]Yamaauchi F. Molecular understanding of soybean protein. Food Rev Intl, 1991, (7): 283-332[3]Ladin B F, Doyle J J, Beachy R N. Molecular characterization of a detection mutation affecting the α′ subunit of β-conglycnin of soybean. J Mol Appl Genet, 1984, 2: 372-380[4]Takahashi K, Banaba H, Kikuchi A, Ito M, Nakamura S. An induced mutant line lacking the α-subunit of β-conglycinin in soybean. Breed Sci, 1994, 46: 251-255[5]Teraishi M, Takahashi M, Hajika M, Matsunaga R, Uematsu Y, Ishimoto M. Suppression of soybean β-conglycinin genes by a dominant gene, Scg-1[J].Theor Appl Genet[6]Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟), Liu Z-X(刘章雄), Guo S-T(郭顺堂). Analysis of protein subunit 7S/11S constitution and allergen lacking of soybean (Glycine max L. Merrill) cultivars. Acta Agron Sin (作物学报), 2004, 30(11): 1076-1079 (in Chinese with English abstract)[7]Ogawa T, Samoto M, Takahashi K. Soybean allergens and hypo-allergenic soybean products. Nutr Sci Vitam, 2000, 46: 271-279[9]Samoto M, Fukuda Y, Takahashi K, Taruchi K, Hiemori M, Tsuji H, Ogawa T, Kawamura Y. Substantially complete removal of three major allergenic soybean proteins (Gly m Bd 30K, Gly m Bd 28, and the α-subunit of β-conglycinin) from soy protein by using a mutant soybean, Tohoku 124 [J].Biosci Biotech Biochem[10]Ladin B F, Doyle J J, Beachy R N. Molecular characterization of a delection mutation affecting the α′ subunit of β-conglycnin of soybean. J Mol Appl Genet, 1984, 2: 372-380[11]Hajika M, Takahashi M, Sakai S, Matsunaga R. Dominant inheritance of a trait lacking β-conglycinin detected in a wild soybean line. Breed Sci, 1998, 48: 383-386[12]Kaizuma N, Kowata H, Odanaka H. Genetic variation on soybean seed proteins induced by irradiation. Rep Tohoku Br Crop Sci Soc Jpn, 1989, 32: 97-99[13]Liu S-S(刘珊珊), Ohta K, Dong C P, Thanh V C, Ishimoto M, Qin Z W, Hirata Y. Genetic diversity of soybean (Glycine max L. Merrill) 7S globulin protein subunits. Genet Resour Crop Evol, 2006, 53: 1209-1219[14]Hajika M, Takahashi M, Sakai S, Igita K. A new genotype of 7S globulin (β-conglycinin) detected in wild soybean (Glycine soja Sied. et. Zucc). Breed Sci, 1996, 46: 385-386[15]Takahashi K, Mizuno Y, Yumoto S, Kitamura K, Nkamura S. Inheritance of the α-subunit deficiency of β-conglycinin in soybean (Glycine max L [J].Merrill) line induced by γ-ray irradiation. Breed Sci[16]Takahashi K, Banba H, Kikuchi A, Ito M, Nakamura S. An induced mutant line lacking the α-subunit of β-conglycinin in soybean (Glycine max L. Merrill). Breed Sci, 1994, 44: 65-66[17]Yagasaki K, Takagi T, Sakai M, Kitamura K. Biochemical characterization of soybean protein consisting of different subunits of glycinin [J].J Agric Food Chem[18]Liu S-S(刘珊珊), Tian F-D(田福东), Gao L-H(高丽辉), Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Li W-B(李文滨). Effect of subunit composition of 7S globulin on soybean quality characteristics [J].Acta Agron Sin (作物学报.2008, 34(5):909-913[19]Liu S-S(刘珊珊), Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Tian F-D(田福东), Gao L-H(高丽辉), Li W-B(李文滨). Correlations between relative content of individual subunit of 7S globulin and quality characteristics in soybean germplasm. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(3): 284-289 (in Chinese with English abstract)[20]Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Li W-B(李文滨). Effect of subunit composition of 7S globulin on soybean quality characteristics. Acta Agron Sin (作物学报), 2008, 34(5): 1-5 (in Chinese with English abstract)[21]Liu S-S(刘珊珊), Diao G-Z(刁桂珠), Wang Z-K(王志坤), Ge Y-J(葛玉君), Wu X-X(武小霞), Gao L-H(高丽辉), Tian F-D(田福东), Li W-B(李文滨). Characterization and evaluation for subun it composition of storage protein in soybean germplasm. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(4): 511-513 (in Chinese with English abstract)[22]Yagasaki K, Kaizuma N, Kitamura K. Inheritance of glycinin subunits and characterization of glycinin molecules lacking the subunits in soybean [Glycine max (L.) Merr]. Breed Sci, 1996, 46: 11-15[23]Meng X-X(孟祥勋). Studies on storage protein in soybean seeds. J Northeast Agric Univ (东北农业大学学报), 1997, 28(2): 201-207 (in Chinese with English abstract)[24]Kitamura K, Davies C S, Nielsen N C. Inheritance of alleles for Cgy1 and Gy4 storage in soybean [J].Theor Appl Genet[25]Tsukada Y K, Kitamura K, Harada K. Genetia analysis of subunits of two major storage protein (β-conglycinin and glycinin) in soybean seeds. Jpn J Breed, 1986, 36: 390-400[26]Chen S, Lin X H, Xu C G, Zhang Q F. Improvement of bacterial resistance of ‘Minghui 63’, an elite restorer line of hybrid rice, by molecular marker-assisted selection[J].Crop Sci[27]Wang X-W(王新望), Lai J-R(赖菁茹), Liu G-T(刘广田). Constructions of elite ph1b winter wheat lines and the application of the marker-assisted selection. Acta Agron Sin (作物学报), 2000, 26(3): 327-333 (in Chinese with English abstract)[28]Xia J-H(夏军红), Zheng Y-L(郑用琏). Molecular marker- assisted backcross breeding of maize Rf3 NIL and its efficient analysis. Acta Agron Sin (作物学报), 2002, 28(3): 339-344 (in Chinese with English abstract)[29]Cregan P B, Mudge J, Fickus E W, Danesh D, Denny R, Young N D. Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by rhg1 locus. Theor Appl Genet, 2000, 99: 811-818 |
| [1] | 金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829. |
| [2] | 唐宽强, 李公允, 宋美毅, 赵雪, 常春玲. 大豆株高性状全基因组关联分析及预测模型构建[J]. 作物学报, 2026, 52(6): 1743-1756. |
| [3] | 姚术, 郭凯悦, 翟慧慧, 姚佳慧, 邓文琪, 闫玲, 黄驰, 高阳, 俞嫣然, 赵振邦, 李英慧, 王晓波, 李佳佳. 大豆苗期耐低铁综合评价及优异种质筛选[J]. 作物学报, 2026, 52(5): 1373-1387. |
| [4] | 郭向阳, 涂亮, 王栋, 刘鹏飞, 王安贵, 易强, 任洪, 李刚, 祝云芳, 吴迅, 蒋喻林, 田丰, 陈泽辉. 热带Suwan种质在我国玉米种质改良中的创新与利用[J]. 作物学报, 2026, 52(3): 655-664. |
| [5] | 张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493. |
| [6] | 王克晶, 李向华. 我国珍稀的大豆属多年生烟豆和短绒野大豆物种遗传资源濒危性评估分析[J]. 作物学报, 2025, 51(8): 2009-2019. |
| [7] | 孟然, 李赵嘉, 冯薇, 陈悦, 刘路平, 杨春燕, 鲁雪林, 王秀萍. 大豆不同生育时期耐盐性综合评价及耐盐种质筛选[J]. 作物学报, 2025, 51(8): 1991-2008. |
| [8] | 贺红利, 张雨涵, 杨静, 程云清, 赵杨, 李星诺, 司洪亮, 张兴政, 杨向东. 大豆e1-as基因突变体的创制及生理分析[J]. 作物学报, 2025, 51(8): 2228-2239. |
| [9] | 胡蒙, 沙丹, 张晟瑞, 谷勇哲, 张世碧, 李静, 孙君明, 邱丽娟, 李斌. 大豆分枝数QTL定位及候选基因筛选[J]. 作物学报, 2025, 51(7): 1747-1756. |
| [10] | 王琼, 邹丹霞, 陈兴运, 张威, 张红梅, 刘晓庆, 贾倩茹, 魏利斌, 崔晓艳, 陈新, 王学军, 陈华涛. 大豆开花时间和成熟期性状全基因组关联分析与候选基因预测[J]. 作物学报, 2025, 51(6): 1558-1568. |
| [11] | 殷丛丛, 李睿琦, 岳霈尧, 李晨, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 基于闭合哑铃介导等温扩增可视化检测大豆花叶病毒SC15方法的建立及应用[J]. 作物学报, 2025, 51(5): 1248-1260. |
| [12] | 许睿, 何妙华, 王昊, 李卫, 任杰, 夏志强. 基于空间转录组技术解析大豆种胚对X射线辐射的响应机制[J]. 作物学报, 2025, 51(12): 3121-3132. |
| [13] | 林洋, 史晓蕾, 陈强, 刘兵强, 杨庆, 于慧娟, 闫龙, 武小霞, 杨春燕. 大豆蛋白质脂肪及脂肪酸组分相关QTL定位[J]. 作物学报, 2025, 51(11): 2899-2910. |
| [14] | 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885. |
| [15] | 李威, 朱玉鹏, 孙宾成, 温有祥, 吴宗声, 徐一帆, 宋雯雯, 徐彩龙, 吴存祥. 转基因大豆结合免耕平作实现东北地区大豆生产轻简化[J]. 作物学报, 2025, 51(10): 2738-2749. |
|
||