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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 87-94.doi: 10.3724/SP.J.1006.2011.00087

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development of Exon-Targeted Intron-Exon Splicing Conjunction (ET-ISJ) Marker and Establishment of Upland Cotton Genetic Map

LIN Gang,ZHANG Jian,ZHANG Ke,TENG Zhong-Hua,ZHANG Zheng-Sheng*   

  1. College of Agronomy and Biotechnology / Key Laboratory of Biotechnology & Crop Quality Improvement, Ministry of Agriculture, Southwest University, Chongqing 400716, China
  • Received:2010-04-16 Revised:2010-07-29 Online:2011-01-12 Published:2010-10-09
  • Contact: ZHANG Zheng-Sheng,E-mail:zhangzs@swu.edu.cn,Tel:13883608797

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Abstract: Cotton is the leading fiber crop in the world, and upland cotton contributes over 95% of cotton production. The genetic map of upland cotton is far from saturated, so it is necessary to develop new markers for it. ET-ISJ (exon targeted intron-exon splice conjunctions) maker primers were designed according to the conserved intron-exon splicing junction sequences. A total of 1 280 ET-ISJ primer combinations were used to screen polymorphism between upland cotton cultivar Yumian 1 and T586, and 69 of which showed polymorphism, accounting for 5.4% of total primer combinations. The 69 polymorphic ET-ISJ primer combinations were used to detect (Yumian 1 × T586) F2:7 recombinant inbred line population, and 70 ET-ISJ loci were obtained. Linkage analysis was conducted on 70 ET-ISJ loci, 523 SSR, 59 IT-ISJ, 29 SRAP and 8 morphological loci, and a linkage map including 59 linkage groups and 673 loci (68 ET-ISJ, 510 SSR, 58 IT-ISJ, 29 SRAP and 8 morphological loci) was established. The linkage map covered 3 216.7 cM, accounting for 72.3% of cotton genome, with an average interval of 4.8 cM between two markers. Sixty-eight ET-ISJ loci were located on 20 chromosomes.The present study demonstrated that ET-ISJ markers are stable, relatively-high polymorphic, and able to be effectively applied in genetic map construction of cotton and other plant’s.

Key words: Upland cotton (Gossypium hirsutum L.), ET-ISJ, Genetic map

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