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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1827-1832.doi: 10.3724/SP.J.1006.2012.01827

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Application of Functional Markers Specific to Powdery Mildew Resistance on Chromosome Arm 6VS from Different Origins of Haynaldia villosa

ZHANG Yun-Long1,2, WANG Mei-Jiao1, ZHANF Yue1, CHU Cui-Ping2, LIN Zhi-Shan1,*, XU Qiong-Fang1, YE Xing-Guo1, CHEN Xiao1,ZHANG Xian-Sheng2   

  1. 1National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China
  • Received:2012-03-21 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 林志珊, E-mail: linzs@caas.net.cn

Abstract:

Lines Pm97033 and 92R137 with good resistance to powdery mildew are wheat–Haynaldia villosa translocation lines carrying 6VS from different H. villosa germplasms. In this study, we identified the similarity of powdery mildew resistance genes in both lines using molecular markers. Based on the sequence of Stpk-V gene (GenBank accession number HQ864471.1), we designed three primer pairs in the coding regions containing at least one intron. Using one pair of primers, a polymorphic fragment of Pm97033 pattern was amplified, which was specific to the alien chromosome arm 6VS. According to the sequencing result of this specific fragment, two new primer pairs were designed for better stability. Primer pair A amplified a specific band specific to 6VS·6DL translocation line Pm97033 and its resistance donor H.v#2. Primer pair B amplified two polymorphic fragments corresponding to 6VS from different H. villosa donors. The effectiveness of primer pairs A and B was then verified in wheat line CB037 with strong powdery mildew resistance, which was developed using both 6V(6D) substitution and 6VS·6AL translocation lines. The banding pattern in CB037 was identical to that in 92R137; however, the amplification fragment of Pm97033 was not observed in CB037. The result of genomic in situ hybridization revealed only one pair of wheat–H. villosa translocation chromosomes in CB037, and the translocation chromosome arm was identified as 6A by molecular markers NAU/xibao15F and NAU/xibao15R. The GISH analysis confirmed the result based on molecular markers designed in this study. Thus, we infer that the powdery mildew resistance in CB037 is derived from 92R137, and CB037 and Pm97033 probably have different genetic bases on resistance to powdery mildew.

Key words: 6VS&bull, 6AL translocation, 6VS&bull, 6DL translocation, Haynaldia villosa, Powdery mildew resistance, Functional marker

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