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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2070-2080.doi: 10.3724/SP.J.1006.2014.02070


Genetic Analysis and Fine Mapping of a Premature Leaf Senescence Mutant in Rice (Orzya sativa L.)

ZHANG Tao1,2,**,SUN Yu-Ying3,**,ZHENG Jian-Min4,**,CHENG Zhi-Jun3,JIANG Kai-Feng1,2,YANG Li1,2,CAO Ying-Jiang1,2,YOU Shu-Mei1,2,WAN Jian-Min3,ZHENG Jia-Kui1,2,5,*   

  1. 1 Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences / Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Deyang 618000, China; 2 Luzhou Branch of National Rice Improvement Center, Luzhou 646100, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 Crop Research Institute,  Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 5 Bioengineering College, Chongqing University, Chongqing 400044, China
  • Received:2013-12-03 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-16
  • Contact: 郑家奎, E-mail: zhen6102@126.com


Leaf senescence induces degradation of chlorophyll and other macromolecules, reducing leaf photosynthetic capacity. This process is accompanied by the accumulation of reactive oxygen species (ROS), the decreasing of cell antioxidant enzyme (SOD, CAT, and APX) activity, and the increasing of aging related gene (SAG) expression, leading in early maturity and yield reduction. Therefore, studies on the genetic mechanism and gene function of premature senescence in rice, has the important effect and significance.in genetic improvement of rice. PLS2 from space radiation mutation breeding project showed leaf senility, at booting stage. Compared with the wild type, in PLS2 the photosynthetic capacity decreased, the plant height, internode and panicle length shortened, tiller and effective tiller number reduced, number of grains per ear and seed setting rate were significantly lower, 1000-grain weight decreased, main panicle was stunted and grain-filling was not full. CAT activity decreased significantly in leaves, H2O2 accumulated, and the number of dead cell increased, chloroplast structures in leaves were worse, with more starches grains and osmiophilic granules. Dark treatment accelerated mutant leaf senescence, chloroplast ultrastructure was spheroidized. Using implicit localization population derived from PLS2/Shuhui 527 and PLS2/02428 the pls2 was located between markers RM14704 (8 674 283 bp) and SL-I-5 (8 758 394 bp) on chromosome 3, with physical distance of 84.11 kb, including 14 genes in the interval. Sequencing result showed that C was replaced by T in the position of 41 base pair of the ninth extron of LOC_Os03g15840 leading to an exchange of Arg (R) is replaced by Cys(C). LOC_Os03g15840 coding a glycosyl transferase (GTs) may be the candidate gene of pls2. These results provide a foundation for the further gene cloning and functional analysis of pls2.

Key words: Leaf senescence, Chloroplast, Map-based cloning, Glycosyltransferase1

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