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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2070-2080.doi: 10.3724/SP.J.1006.2014.02070

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Fine Mapping of a Premature Leaf Senescence Mutant in Rice (Orzya sativa L.)

ZHANG Tao1,2,**,SUN Yu-Ying3,**,ZHENG Jian-Min4,**,CHENG Zhi-Jun3,JIANG Kai-Feng1,2,YANG Li1,2,CAO Ying-Jiang1,2,YOU Shu-Mei1,2,WAN Jian-Min3,ZHENG Jia-Kui1,2,5,*   

  1. 1 Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences / Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Deyang 618000, China; 2 Luzhou Branch of National Rice Improvement Center, Luzhou 646100, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 Crop Research Institute,  Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 5 Bioengineering College, Chongqing University, Chongqing 400044, China
  • Received:2013-12-03 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-16
  • Contact: 郑家奎, E-mail: zhen6102@126.com

Abstract:

Leaf senescence induces degradation of chlorophyll and other macromolecules, reducing leaf photosynthetic capacity. This process is accompanied by the accumulation of reactive oxygen species (ROS), the decreasing of cell antioxidant enzyme (SOD, CAT, and APX) activity, and the increasing of aging related gene (SAG) expression, leading in early maturity and yield reduction. Therefore, studies on the genetic mechanism and gene function of premature senescence in rice, has the important effect and significance.in genetic improvement of rice. PLS2 from space radiation mutation breeding project showed leaf senility, at booting stage. Compared with the wild type, in PLS2 the photosynthetic capacity decreased, the plant height, internode and panicle length shortened, tiller and effective tiller number reduced, number of grains per ear and seed setting rate were significantly lower, 1000-grain weight decreased, main panicle was stunted and grain-filling was not full. CAT activity decreased significantly in leaves, H2O2 accumulated, and the number of dead cell increased, chloroplast structures in leaves were worse, with more starches grains and osmiophilic granules. Dark treatment accelerated mutant leaf senescence, chloroplast ultrastructure was spheroidized. Using implicit localization population derived from PLS2/Shuhui 527 and PLS2/02428 the pls2 was located between markers RM14704 (8 674 283 bp) and SL-I-5 (8 758 394 bp) on chromosome 3, with physical distance of 84.11 kb, including 14 genes in the interval. Sequencing result showed that C was replaced by T in the position of 41 base pair of the ninth extron of LOC_Os03g15840 leading to an exchange of Arg (R) is replaced by Cys(C). LOC_Os03g15840 coding a glycosyl transferase (GTs) may be the candidate gene of pls2. These results provide a foundation for the further gene cloning and functional analysis of pls2.

Key words: Leaf senescence, Chloroplast, Map-based cloning, Glycosyltransferase1

[1]Navabpour S, Morris K, Allen R, Harrison E, A-H-Mackerness S, Buchanan-Wollaston V. Expression of senescence-enhanced genes in response to oxidative stress. J Exp Bot, 2003, 54: 2285–2292



[2]袁政, 张大兵. 植物叶片衰老的分子机制. 植物生理学通讯, 2002, 38: 417–422



Yuan Z, Zhang D B. The molecular mechanism of leaf senescence. Plant Physiol Commun, 2002, 38(4): 417–422 (in Chinese with English abstract)



[3]He Y, Tang W, Swain J D, Green A L, Jack T P, Gan S. Networking senescence-regulating pathways by using Arabidopsis enhancer trap lines. Plant Physiol, 2001, 126: 707–716



[4]Eckardt N A. A new chlorophyll degradation pathway. Plant Cell, 2009, 21: 700–700



[5]Lim P O, Kim H J, Nam H G. Leaf senescence. Annu Rev Plant Biol, 2007, 58: 115–136



[6]Cha K W, Lee Y J, Koh H J, Lee B M, Nam Y W, Paek N C. Isolation, characterization, and mapping of stay green mutant in rice. Theor Appl Genet, 2002, 104: 526–532



[7]Lee R H, Lin M C, Chen S C. A novel alkaline a-galactosidase gene is involved in rice leaf senescence. Plant Mol Biol, 2004, 55: 281–295



[8]Ansari M I, Lee R H, Chen S C. A novel senescence-associated gene encoding GABA: pyruvate transaminase is up-regulated during rice leaf senescence. Physiol Plant, 2005, 123: 1–8



[9]Kong Z S, Li M N, Yang W Q, Xu W Y, Xue Y B. A novel nuclear localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice. Plant Physiol, 2006, 141: 1376–1388



[10]Jiang H W, Li M R, Liang N T, Yan H B, Wei Y B, Xu X L, Liu J, Xu Z F, Chen F, Wu G J. Molecular cloning and function analysis of the stay green gene in rice. Plant J, 2007, 52: 197–209



[11]Park S Y, Yu J W, Park J S, Li J J, Yoo S C, Lee N Y, Lee S K, Jeong S W, Seo H S, Koh H J, Jeon J S, Park Y, Paek N C. The senescence-induced stay green protein regulates chlorophyll degradation. Plant Cell, 2007, 19: 1649–1664



[12]Kusaba M, Ito H, Morita R, Iida S, Sato Y, Fujimoto M, Kawasaki S, Tanaka R, Hirochika H, Nishimura M, Tanaka A. Rice NON-YELLOW COLORINGI is involved in light-harvesting complex II and grana degradation during leaf senescence. Plant Cell, 2007, 19: 1362–1375



[13]Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40



[14]Qiao Y L, Jiang W Z, Lee J H, Park B S, Choi M S, Piao R H, Woo M O, Roh J H, Han L Z, Paek N C, Seo H S, Koh H J. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol, 2009, 185: 258–274



[15]Li F Z, Hu G C, Fu Y P, Si H M, Bai X M, Sun Z X. Genetic analysis and high-resolution mapping of premature senescence gene Pse(t) in rice (Oryza sativa L.). Genome, 2005, 48: 738–746



[16]Wang J, Wu S J, Zhou Y, Zhou L H, Xu J F, Hu J, Fang Y X, Gu M H, Liang G H. Genetic analysis and molecular mapping of a presenescing leaf gene pls1 in rice (Oryza sativa L.). Chin Sci Bull, 2006, 51: 2986–2992



[17]Yan W Y, Ye S, Jin Q S, Zeng L J, Peng Y, Yan D W, Yang W B, Yang D L, He Z H, Dong Y J, Zhang X M. Characterization and mapping of a novel mutant sms1 (senescence and male sterility 1) in rice. J Genet Genomics, 2010, 37: 47–55



[18]Fang L K, Li Y F, Gong X P, Sang X C, Ling Y H, Wang X W, Cong Y F, He G H. Genetic analysis and high-resolution mapping of pls3 (presenescing leaf 3) in rice. Chin Sci Bull, 2010, 55: 1676–1681



[19]Yang Y L, Rao Y C, Liu H J, Fang Y X, Dong G J, Huang L C, Leng Y J, Guo L B, Zhang G H, Hu J, Gao Z Y, Qian Q, Zeng D L. Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L. Chin Sci Bull, 2011, 56: 2437−2443



[20]杜青, 方立魁, 桑贤春, 凌英华, 李云峰, 杨正林, 何光华, 赵芳明. 水稻叶尖早衰突变体lad的形态、生理分析与基因定位. 作物学报, 2012, 38: 168–173



Du Q, Fang L K, Sang X C, Ling Y H, Li Y F, Yang Z L, He G H, Zhao F M. Analysis of phenotype and physiology of leaf apex dead mutant (lad) in rice and mapping of mutant gene. Acta Agron Sin, 2012, 38: 168–173 (in Chinese with English abstract)



[21]徐芳芳, 桑贤春, 任德勇, 唐彦强, 胡宏伟, 杨正林, 赵芳明, 何光华. 水稻早衰突变体esl2的遗传分析和基因定位. 作物学报, 2012, 38: 1347–1353



Xu F F, Sang X C, Ren D Y, Tang Y Q, Hu H W, Yang Z L, Zhao F M, He G H. Genetic analysis and gene mapping of early senescence leaf mutant esl2 in rice. Acta Agron Sin, 2012, 38: 1347–1353 (in Chinese with English abstract)



[22]苗润隆, 蒋钰东, 廖红香, 徐芳芳, 何光华, 杨正林, 赵芳明, 桑贤春. 水稻早衰突变体esl3的鉴定与基因定位. 作物学报, 2013, 39: 862–867



Miao R L, Jiang Y D, Liao H X, Xu F F, He G h, Yang Z L, Zhao F M, Sang X C. Identification and gene mapping of rice early senescent leaf (esl3) mutant. Acta Agron Sin, 2013, 39: 862–867 (in Chinese with English abstract)



[23]王复标, 黄福灯, 程方民, 李兆伟, 胡东维, 潘刚, 毛愉婵. 水稻生育后期叶片早衰突变体的光合特性与叶绿体超微结构观察. 作物学报, 2012, 38: 871–879



Wang F B, Huang F D, Cheng F M, Li Z W, Hu D W, Pan G, Mao Y C. Photosynthesis and chloroplast ultra-structure characteristics of flag leaves for a premature senescence rice mutant. Acta Agron Sin, 2012, 38: 871–879



[24]Undan J R, Tamiru M, Abe A, Yoshida K, Kosugi S, Takagi H, Yoshida K, Kanzaki H, Saitoh H, Fekih R. Mutation in OsLMS, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (Oryza sativa L.). Genes Genet Syst, 2012, 87: 169–179



[25]Lin A H, Wang Y Q, Tang J Y, Xue P, Li C L, Liu L C, Hu B,Yang F Q, Loake G J, Chu C C. Nitric oxide and protein S-nitrosylation are integral to hydrogen peroxide induced leaf cell death in rice. Plant Physiol, 2012, 158: 451−464



[26]张涛, 郑家奎, 蒋开锋, 郑建敏, 杨乾华, 杨莉, 万先齐, 曹应江. 水稻航天衰老突变体基因psl2的表型和遗传分析. 分子植物育种, 2010, 8: 245–251



Zhang T, Zhen J K, Jiang K F, Zheng J M, Yang Q H, Yang L, Wan X Q, Cao Y J. Phenotypes and genetic analysis of a senescence mutant by aeronautics in rice. Mol Plant Breed, 2010, 8: 245–251(in Chinese with English abstract)



[27]张涛. 水稻糙米蛋白质含量的QTL定位及香稻的资源研究. 四川农业大学博士学位论文, 四川雅安, 2007. p 25



Zhang T. QTL Mapping of Brown Rice Protein Content in a RIL Population of Rice and Analysis of Genetic Diversity of Aromatic Rice Varieties. PhD Dissertation of Sichuan Agricultural University, Sichuan, China, 2007. p 25(in Chinese with English abstract)



[28]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69–76



[29]Lin Q B, Wang D, Dong H, Gu S H, Cheng Z J, Gong J, Qin R Z, Jiang L, Li G, Wang J L, Wu F Q, Guo X P, Zhang X, Lei C L, Wang H Y, Wan J M. Rice APC/CTE controls tillering by mediating the degradation of MONOCULM1. Nat Commun, 2012, 3: 752, DOI: 10.1038/ncomms1716 www.nature.com/naturecommunications



[30]Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim P O,Nam H G, Lin J F, Wu S H, Swidzinski J, Ishizaki K, Leaver C J. Comparative transcriptome analysis reveals significant differences in gene expression and signaling pathways between developmental and dark/starvation induced senescence in Arabidopsis. Plant J, 2005, 42: 567–585



[31]马跃芳, 陆定志. 灌水方式对杂交水稻衰老及生育后期一些生理活性的影响. 中国水稻科学, 1990, 4(2): 56–62



Ma Y F, Lu D Z. Effect of irrigation modes on the senescence and physiological activity in hybrid rice after heeding. Chin J Rice Sci, 1990, 4(2): 56–62 (in Chinese with English abstract)



[32]Woo H H, Jeong B R, Koo K B, Choi J W, Hirsch A M, Hawes M C. Modifying expression of closely related UDP-glycosyltransferases from pea and Arabidopsis results in altered root development and function. Physiol Plant, 2007, 130: 250–260

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