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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (9): 1367-1379.doi: 10.3724/SP.J.1006.2018.01367

• RESEARCH PAPERS • Previous Articles     Next Articles

Cloning and Expression Characteristic Analysis of ScWRKY4 Gene in Sugarcane

Ling WANG1(),Feng LIU1,Ming-Jian DAI1,Ting-Ting SUN1,Wei-Hua SU1,Chun-Feng WANG1,Xu ZHANG1,Hua-Ying MAO1,Ya-Chun SU1,2,*(),You-Xiong QUE1,2,*()   

  1. 1 Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture / Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    2 Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization, Ministry of Education / Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;
  • Received:2018-01-28 Accepted:2018-06-12 Online:2018-09-10 Published:2018-07-02
  • Contact: Ya-Chun SU,You-Xiong QUE E-mail:lingw2017@126.com;syc2009mail@163.com;queyouxiong@126.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31671752);This study was supported by the National Natural Science Foundation of China(31101196);Natural Science Foundation of Fujian Province for Distinguished Young Scholars(2015J06006);Research Funds for Distinguished Young Scientists in Fujian Provincial Department of Education(SYC-2017);China Agriculture Research System (CARS-17)

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Abstract:

WRKY is one of the specific transcriptional regulators in plants and widely involved in the response of plants to diverse biotic and abiotic stresses. In this study, based on our previous transcriptome data of sugarcane (Saccharum spp.), we successfully cloned a WRKY gene from sugarcane variety ROC22, named as ScWRKY4 (GenBank accession number MG852087). Sequence analysis showed that the full-length cDNA of ScWRKY4 gene was 1265 bp, containing a 741 bp complete open reading frame and encoding 246 amino acids residues. ScWRKY4 protein had a conservative WRKYGQK domain and a zinc finger motif C2H2, belonging to IIc subgroup of WRKY transcription factor family. Bioinformatics analysis indicated that ScWRKY4 was an alkaline unstable hydrophilic protein with no signal peptide and transmembrane structure. The secondary structural element of ScWRKY4 protein was lack of beta helix structure. ScWRKY4 was located in the nucleus after transient expression in Nicotiana benthamiana. The result of yeast hybridization experiment demonstrated that ScWRKY4 did not possess transcriptional activity. Real-time fluorescent quantitative PCR showed that the expression of ScWRKY4 gene had no significant difference in root, leaf, bud and stem epidermis, while had the highest expression level in stem pith, which was 18.38 times higher than that in root. During inoculation with smut pathogen (Sporisorium scitamineum) at 0-72 h, the gene expression level of ScWRKY4 was down-regulated in sugarcane smut-resistant cultivar Yacheng 05-179, while was more stable in the susceptible one ROC22. The transcript of ScWRKY4 was up-regulated by exogenous hormone stresses of abscisic acid, salicylic acid, and methyl jasmonate, as well as by the abiotic treatments of sodium chloride and polyethylene glycol. These results suggest that ScWRKY4 gene may not participate in sugarcane smut resistance, or only play a negative role in this defense reaction, but positively involve in the response mechanism of salt and drought resistance in sugarcane.

Key words: sugarcane, WRKY transcription factor, bioinformatics, subcellular localization, transcriptional activity, real-time flourescent quantitative PCR

Table 1

Sequence and usage of primers"

引物名称
Primer name		 引物序列
Primer sequence (5°-3°)		 用途
Usage
ScWRKY4-F TCCTCGGCATCTCCCATTCT 扩增全长
Full length amplifcation
ScWRKY4-R GTCTGGGAGCTCATGTTCGT
ScWRKY4-QF ATGAAGGTGAGGAGGAAGATG 荧光定量
qRT-PCR
ScWRKY4-QR CTTGTAGCCGTCGTCCAG
GAPDH-QF CACGGCCACTGGAAGCA 内参基因
Reference gene
GAPDH-QR TCCTCAGGGTTCCTGATGCC
ScWRKY4-Subloc-F CGGGATCCATGGAGGGGAGCA 亚细胞定位
Subcellular localization
ScWRKY4-Subloc-R TGCTCTAGAGAGCGACGTGAAAGC
ScWRKY4-BD-F GGAATTCCATATGATGGAGGGGAGCAGCCAGCT 转录激活
Transactivation
ScWRKY4-BD-R CGGGATCCGAGCGACGTGAAAGCGCAGC

Fig. 1

PCR amplification of ScWRKY4 gene in sugarcaneM: 15000+2000 bp DNA marker; 1: PCR product."

Fig. 2

Nucleotide acid sequences and deduced amino acid sequences of sugarcane ScWRKY4 gene obtained by PCR amplification (*: stop codon)The sequence of WRKYGQK motif is highlighted in red box, and that of C2H2 motif (CX4CX23HXH) in black box."

Fig. 3

Conserved domain prediction of ScWRKY4 protein"

Fig. 4

Sequence alignment of ScWRKY4 and WRKYs in other speciesSorghum bicolor: SbWRKY57 (XP_002448267.2); Zea mays: ZmWRKY36 (NP_001151912.1); Dichanthelium oligosanthes: DoWWRKY12 (OEL21317.1); Setaria italic: SiWRKY12 (XP_004956420.1); Brachypodium distachyon: BdWRKY12 (XP_014751531.1); Oryza sativa OsWRKY12 (XP_015635033.1)."

Fig. 5

Phylogenetic tree of ScWRKY4 protein and WRKYs in sugarcane and other species"

Fig. 6

Subcellular localization assay of sugarcane ScWRKY4 (A) The enzyme digestion to identify the insert-integrated subcellular localization expression vector 35S::ScWRKY4::GFP. 1: 15000+2000 bp DNA marker; 2: 35S::GFP/Xba I; 3: ScWRKY4 ORF PCR product; 4: 35S::ScWRKY4::GFP/Xba I; 5: 35S::ScWRKY4::GFP/Xba I+BamH I; 6: 100 bp ladder DNA marker. (B) Subcellular localizations of the Agrobacterium mediated transformation of ScWRKY4 and empty vector in Nicotiana benthamiana leaves 48 h after infiltration. The epidermal cells of N. benthamiana were used for taking images of visible light, green fluorescence, blue fluorescence, merged visible light and green fluorescence and blue fluorescence; white arrows 1, 2, and 3 indicate plasma membrane, nucleus and cytoplasm, respectively; scale bar = 25 μm; 35S::GFP: the Agrobacterium tumefaciens strain carrying the empty vector pCAMBIA 1300-GFP; 35S::ScWRKY4::GFP: the A. tumefaciens strain carrying the recombinant vector pCAMBIA 1300-ScWRKY4-GFP; DAPI: 4’,6-diamidino-2-phenylindole."

Fig. 7

Transactivation activity assay of ScWRKY4 in sugarcane (A) The enzyme digestion to identify the insert-integrated vector pGBKT7-ScWRKY4. 1: 15000+2000 bp DNA marker; 2: pGBKT7/Nde I; 3: ScWRKY4 ORF PCR product; 4: pGBKT7-ScWRKY4/Nde I; 5: pGBKT7-ScWRKY4/Nde I+BamH I; 6: 2000 bp ladder DNA marker. (B) The test of transactivation activity assay of ScWRKY4. SD/-Trp: synthetic dropout medium plate; SD/-Trp (+ X-α-Gal): synthetic dropout medium plate without tryptophan (plus 5-bromo-4-chloro-3-indoxyl-α-D-galactopyranoside); SD/-Trp (+ X-α-Gal+ AbA): synthetic dropout medium plate without tryptophan (plus 5-bromo-4-chloro-3-indoxyl-α-D-galactopyranoside and aureobasidin A)."

Fig. 8

Specificity analysis of ScWRKY4 gene in sugarcane ROC22 tissueBars superscripted by different lowercase letters are significantly different (P ≤ 0.05); n = 3; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference gene; R: root; B: bud; L: leaf; SP: stem pith; SE: stem epidermis."

Fig. 9

Expression of sugarcane ScWRKY4 gene under the infection of smut pathogenYacheng 05-179: smut-resistant sugarcane variety; ROC22: smut-susceptible sugarcane variety. Bars superscripted by different lowercase letters are significantly different (P ≤ 0.05); n = 3; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference gene."

Fig. 10

Relative expression level of the sugarcane ScWRKY4 gene under different plant hormones and abiotic stress treatmentsBars superscripted by different lowercase letters are significantly different (P ≤ 0.05); n = 3; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference gene; SA: salicylic acid (5 mmol L-1); MeJA: methyl jasmonate (25 μmol L-1); NaCl: sodium chloride (simulating salt stress) (250 mmol L-1); PEG: polyethylene glycol (simulating drought treatment) (25.0%); ABA: abscisic acid (100 μmol L-1)."

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