Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1122-1127.doi: 10.3724/SP.J.1006.2017.01122

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization and Gene Mapping of sostenuto floret opening 1 (sfo1) Mutant in Rice (Oryza sativaL.)

SHEN Ya-Lin,ZHUANG Hui,CHEN Huan,ZENG Xiao-Qin,LI Xiang-Ning,ZHANG Jun,ZHENG Hao, LING Ying-Hua,LI Yun-Feng*   

  1. Rice Research Institute, Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400716, China
  • Received:2016-12-01 Revised:2017-04-20 Online:2017-08-12 Published:2017-04-27
  • Contact: Li Yuefeng, E-mail: liyf1980@swu.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271304) and Fundamental Research Funds for the Central Universities (XDJK2016A013).

RichHTML

PDF

806

Abstract:

Rice floret opening is one of the most critical physiological processes in its reproductive development, which has a significant influence on the fertilization and subsequent seed development. Rice floret opening and closing are promoted respectively by the lodicules’ expansion and shrink. In recent years, many studies focused on the molecular mechanism of rice lodiculedevelopment.However, few studies focused on the molecular regulation mechanism in physiological process of lodicule opening and closing. In this paper, we reported a floret opening mutant, sostenuto floret opening 1 (sfo1), derived from EMS (ethyl methane sulfonate) mutation groups of Xinong 1B.During the floret opening stage, the florets of sfo1 and wild type were observed directly by both the stereoscope and the scanning electron microscopy at about 1 h before opening, and 10 min, 1.5 h and 48 h after openingrespecting. Compared withthat in the wild type, the sfo1floret showed a delay of lodicules dehydration after floret opening, resulting in its failing to close the lemma and palea. Significant differenceswere not found at1h before opening, showing closed hulls, flat lodicules and well-stackedlodicules surface cells.At 10 min after opening, there were also no obvious differences between wild type and mutants,showing openinghulls, inflated lodicules, and smooth and well-stacked surface cells. At1.5 h after opening, sfo1 hulls were not closed so big as those in the wild type. And sfo1lodicules still inflated, but not dehydrated and atrophied as those in thewild type. At 48 h after opening, sfo1hullswere not closed yet, its lodiculesbegan to dehydrate and atrophy, but were still very full compared with those in the wild type. The F1and F2 of across with sterile lines 56S as female parent and the sfo1 as male parent wereused in genetic analysis and gene mapping, indicating that the sfo1 trait was controlled by a nuclear recessive gene. Using bulked segregation analysis (BSA) method, the SFO1 was located between the SSR marker RM1054 and Insert/Delete marker ZTQ51 on the chromosome 5, with a physical distance of 113kb and including 15 candidate genes in this region. LOC_Os05g50890 and LOC_Os05g50900 were preliminary selected as candidate genes for SFO1. These results laid a foundation for the SFO1 gene cloning and functional research.

Key words: Rice (oryza sativa), floret opening, Lodicule, Gene mapping

[1]曾晓春, 周燮, 吴晓玉. 水稻颖花开放机理研究进展. 中国农业科学, 2004, 37: 188–195
Zeng X C, Zhou X, Wu X Y. Advances in study of opening mechanism in rice florets. Sci Agric Sin, 2004, 37:188–195 (in Chinese with English abstract)
[2]许祥明, 王忠, 顾蕴洁, 高珠. 某些酸类物质对水稻开颖的效应. 植物生理学报, 1998, 24: 124–130
Xu X M, Wang Z, Gu W J, Gao Z. Effects of some acids on rice glume opening. Plant Physiol J, 1998, 24: 124–130 (in Chinese with English abstract)
[3]Yadav S R, Prasad K, Vijayraghavan U. Divergent regulatory OsMADS2 functions control size, shape and differentiation ofthe highly derived rice floret second-whorl organ. Genetics, 2007,176: 283–294
[4]Yao S G, Ohmori S, Kimizu M, Yoshida H. Unequal genetic redundancy of rice PISTILLATA orthologs, OsMADS2 andOsMADS4, in lodicule and stamen development. Plant Cell Physiol, 2008, 49: 853–857
[5]Xiao H, Wang Y, Liu D F, Wang W M, Li X B, Zhao X F, Xu J C, Zhai W X, Zhu L H. Functional analysis of the rice AP3homologue OsMADS16 by RNA interference. Plant Mol Biol, 2003, 52: 957–966
[6]Li H F, Liang W Q, Jia R D, Yin C S, Zong J, Kong H Z, Zhang D B. The AGL6-like gene OsMADS6 regulates floral organand meristem identities in rice. Cell Res, 2010, 20: 299–313
[7]Nam J, Kim J, Lee S, An G, Ma H, Nie M. Type I MADS-box genes have experienced faster birth-and-death evolution than type II MADS-box genes in angiosperms. Proc Natl Acad SciUSA, 2004, 101: 1910–1915
[8]Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z. STAMENLESS1, encoding asingle C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 5: 789–801
[9]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proc Natl Acad SciUSA, 1991, 88: 9828–9832
[10]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321–4325
[11]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10(t) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817
[12]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37
[13]Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78: 203–209
[14]Theissen G, Saedler H. Plant biology: floral quartets. Nature, 2001, 409: 469–471
[15]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, NagatoY. SUPERWOMAN1andDROOPING LEAF genescontrol floral organ identity in rice. Development, 2003, 130: 705−718
[16]Yoshida H, Itoh J I, Ohmori S, Miyoshi K, Horigome A, Uchida E, Kimizu M, Matsumura Y, Kusaba M, Satoh H, Nagato Y. Superwoman1-cleistogamy, a hopeful allele for gene containment in GM rice. Plant Biotechnol J, 2007, 5: 835–846
[17]黄俊宝, 何永明, 曾晓春, 向妙莲, 付永琦. 水稻颖花开放前花器官茉莉酸水平变化及浆片茉莉酸信号基因表达分析. 中国农业科学, 2015, 48: 1219–1227
Huang J B, He Y M, Zeng X C, Xiang M L, Fu Y Q. Changes of JA levels in floral organs and expression analysis of JA signaling genes in lodicules before floret opening in rice. Sci Agric Sin, 2015, 48: 1219–1227 (in Chinese with English abstract)
[18]Ohashi-Ito K, Bergmann D C. Arabidopsis FAMA controls the final proliferation/differentiation switch during stomatal development. Plant Cell, 2006, 18: 2493–2505
[1] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[2] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
[3] XU Ning-Kun, LI Bing, CHEN Xiao-Yan, WEI Ya-Kang, LIU Zi-Long, XUE Yong-Kang, CHEN Hong-Yu, WANG Gui-Feng. Genetic analysis and molecular characterization of a novel maize Bt2 gene mutant [J]. Acta Agronomica Sinica, 2022, 48(3): 572-579.
[4] JIANG Cheng-Gong, SHI Hui-Min, WANG Hong-Wu, LI Kun, HUANG Chang-Ling, LIU Zhi-Fang, WU Yu-Jin, LI Shu-Qiang, HU Xiao-Jiao, MA Qing. Phenotype analysis and gene mapping of small kernel 7 (smk7) mutant in maize [J]. Acta Agronomica Sinica, 2021, 47(2): 285-293.
[5] GUO Qing-Qing, ZHOU Rong, CHEN Xue, CHEN Lei, LI Jia-Na, WANG Rui. Location and InDel markers for candidate interval of the orange petal gene in Brassica napus L. by next generation sequencing [J]. Acta Agronomica Sinica, 2021, 47(11): 2163-2172.
[6] HUANG Yan, HE Huan-Huan, XIE Zhi-Yao, LI Dan-Ying, ZHAO Chao-Yue, WU Xin, HUANG Fu-Deng, CHENG Fang-Min, PAN Gang. Physiological characters and gene mapping of a dwarf and wide-leaf mutant osdwl1 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(1): 50-60.
[7] JIANG Hong-Rui, YE Ya-Feng, HE Dan, REN Yan, YANG Yang, XIE Jian, CHENG Wei-Min, TAO Liang-Zhi, ZHOU Li-Bin, WU Yue-Jin, LIU Bin-Mei. Identification and gene localization of a novel rice brittle culm mutant bc17 [J]. Acta Agronomica Sinica, 2021, 47(1): 71-79.
[8] SHI Hui-Min, JIANG Cheng-Gong, WANG Hong-Wu, MA Qing, LI Kun, LIU Zhi-Fang, WU Yu-Jin, LI Shu-Qiang, HU Xiao-Jiao, HUANG Chang-Ling. Phenotype identification and gene mapping of defective kernel 48 mutant (dek48) in maize [J]. Acta Agronomica Sinica, 2020, 46(9): 1359-1367.
[9] TIAN Shi-Ke, QIN Xin-Er, ZHANG Wen-Liang, DONG Xue, DAI Ming-Qiu, YUE Bing. Genetic analysis and characterization of male sterile mutant mi-ms-3 in maize [J]. Acta Agronomica Sinica, 2020, 46(12): 1991-1996.
[10] XIE Yuan-Hua,LI Feng-Fei,MA Xiao-Hui,TAN Jia,XIA Sai-Sai,SANG Xian-Chun,YANG Zheng-Lin,LING Ying-Hua. Phenotype characterization and gene mapping of the semi-outcurved leaf mutant sol1 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2020, 46(02): 204-213.
[11] MO Yi,SUN Zhi-Zhong,DING Jia,YU Dong,SUN Xue-Wu,SHENG Xia-Bing,TAN Yan-Ning,YUAN Gui-Long,YUAN Ding-Yang,DUAN Mei-Juan. Genetic analysis and fine mapping of white stripe leaf mutant wsl1 in rice [J]. Acta Agronomica Sinica, 2019, 45(7): 1050-1058.
[12] Rui WANG,Yang-Song CHEN,Ming-Hao SUN,Xiu-Yan ZHANG,Yi-Cong DU,Jun ZHENG. Genetic analysis and causal gene identification of maize viviparous mutant vp-like8 [J]. Acta Agronomica Sinica, 2019, 45(5): 656-661.
[13] Li-Na SHANG,Xin-Long CHEN,Sheng-Nan MI,Gang WEI,Ling WANG,Ya-Yi ZHANG,Ting LEI,Yong-Xin LIN,Lan-Jie HUANG,Mei-Dan ZHU,Nan WANG. Phenotypic identification and gene mapping of temperature-sensitive green- revertible albino mutant tsa2 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2019, 45(5): 662-675.
[14] ZHANG Li-Sha,MI Sheng-Nan,WANG Ling,WEI Gang,ZHENG Yao-Jie,ZHOU Kai,SHANG Li-Na,ZHU Mei-Dan,WANG Nan. Physiological and biochemical analysis and gene mapping of a novel short radicle and albino mutant sra1 in rice [J]. Acta Agronomica Sinica, 2019, 45(4): 556-567.
[15] WANG Xiao-Juan,PAN Zhen-Yuan,LIU Min,LIU Zhong-Xiang,ZHOU Yu-Qian,HE Hai-Jun,QIU Fa-Zhan. Genetic analysis and molecular characterization of a new allelic mutant of silky1 gene in maize [J]. Acta Agronomica Sinica, 2019, 45(11): 1649-1655.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd