Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (11): 1735-1745.doi: 10.3724/SP.J.1006.2019.94022

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Establishment of an accurate evaluation method for drought resistance based on multilevel phenotype analysis in sorghum

ZHANG Xiao-Xiao1,2,PAN Ying-Hong3,REN Fu-Li2,PU Wei-Jun2,WANG Dao-Ping3,LI Yu-Bin2,LU Ping3,LI Gui-Ying3,*(),ZHU Li2,*()   

  1. 1 Southwest University of Science and Technology, Mianyang 621000, Sichuan, China
    2 Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-02-14 Accepted:2019-05-12 Online:2019-11-12 Published:2019-06-04
  • Contact: Gui-Ying LI,Li ZHU E-mail:liguiying@caas.cn;zhuli01@caas.cn
  • Supported by:
    This study was supported by the Fundamental Research Funds for Central Non-profit Scientific Institution(Y2017PT25);the National Natural Science Foundation of China(31471558);the National Key R&D Program of China(2018YFD1000702);the IAEA Coordinated Research Project(D23031)

RichHTML358

6

PDF

371

Abstract:

Drought is one of the important abiotic stresses that restrict agricultural production. Research on drought resistance of crops is of significance in both theoretical and practical aspects. In this study, 73 sorghum accessions were preliminarily evaluated for drought resistance at the germination and seedling stages under simulated drought environment with PEG-6000. Among them, 21 accessions including 15 tolerant and 6 susceptible, were screened to measure the morphological characteristics such as plant height, leaf length and leaf width, and physiological and biochemical indicators such as superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and peroxidase (POD) activity. Finally, one accession was identified with high drought resistance, one accession was moderate drought resistance and two accessions with drought-sensitivity. A new evaluation method for sorghum drought resistance was established based on the multilevel phenotypic analysis, in which the data from subordinate function method, principal component analysis and clustering analysis were comprehensively applied, and combined with various phenotypic data (morphological, physiological and biochemical indicators) at different growth stages (germination and seedling stages) in different growth environments (field test and laboratory test). This method can be used to systematically, efficiently and accurately identify the drought resistance of sorghum based on the consistency of evaluation results by multiple comparison analysis, which is useful in the research on drought resistance mechanism and sorghum breeding for drought resistance.

Key words: multilevel phenotypic analysis, sorghum, drought resistance, germination stage, seedling stage

Fig. 1

Scheme of multilevel phenotypic analysis methods"

Table 1

Information of 73 sorghum accessions"

编号
Number		 名称
Name		 编号
Number		 名称
Name
D01 Club.S D38 SEM-20-4
D02 IS-8377 D39 SEM-20-5
D03 5933 D40 SEM-28-4
D04 M81-E D41 SEM-30-1
D05 SEM-2-4 D42 SEM-31-4
D06 灯笼红(忻州) Denglonghong (Xinzhou) D43 SEM-32-1
D07 黑暴糯高粱(广南) Heibaonuogaoliang (Guangnan) D44 SEM-32-2
D08 Bird proof kafer #-662 D45 SEM-32-4
D09 矮脚糯(平利) Aijiaonuo (Pingli) D46 SEM-32-5
D10 糯高粱(宁夏) Nuogaoliang (Ningxia) D47 SEM-32-9
D11 糯高粱(柞水) Nuogaoliang (Zuoshui) D48 SEM-32-4
D12 CLUBHEAD-3 D49 SEM-32-5
D13 甜131 Tian 131 D50 SEM-32-9
编号
Number		 名称
Name		 编号
Number		 名称
Name
D14 千斤锤(沁水) Qianjinchui (Qinshui) D51 SEM-38-4
D15 甜选29 Tianxuan 29 D52 SEM-38-7
D16 甜选37 Tianxuan 37 D53 SEM-42-1
D17 甜选129 Tianxuan 129 D54 SEM-44-2
D18 Icsv 219 D55 SEM-54-1
D19 木浦在来种Mupuzailaizhong D56 SEM-54-5
D20 IS-9180 D57 SEM-21-8
D21 10048 D58 SEM-24-6
D22 AIRI-2 D59 SEM-24-7
D23 东北农信糯Dongbeinongxinnuo D60 SEM-30-3
D24 河北糯杂5号Heibeinuoza 5 D61 SEM-49-2
D25 河北糯杂6号Hebeinuoza 6 D62 SEM-49-4
D26 红茅糯2号Hongmaonuo 2 D63 SEM-51-11
D27 山东莱阳糯Shandonglaiyangnuo D64 SEM-53-4
D28 原15B Yuan 15B D65 SEM-53-5
D29 耘糯Yunnuo D66 SEM-53-7
D30 上海甜Shanghaitian D67 SEM-53-10
D31 软高粱(定襄) Ruangaoliang (Dingxiang) D68 SEM-57-4
D32 米高粱(清涧) Migaoliang (Qingjian) D69 SEM-56-8
D33 CP16-1481 D70 SEM-62-5
D34 SEM-8-4 D71 SEM-66-2
D35 SEM-16-10 D72 科甜5号Ketian 5
D36 SEM-17-3 D73 SEM-42-4
D37 SEM-20-2

Fig. 2

Subordinate function value of drought resistance index at germination stage and wilting index level of different sorghum accessions at seedling stage RGP: relative germination potential; RGR: relative germination rate; DGRI: germination drought resistance index; ASFV: average subordinative function value; 1-WI: 1-wilting index level assignment at seedling stage."

Table 2

Comprehensive evaluation of drought resistance of 21 sorghum accessions at seedling stage based on subordinate function method"

材料编号
Accession number		 田间抗旱性排序
Field drought
resistance ranking		 室内抗旱性排序
Laboratory drought resistance ranking		 材料编号
Accession number		 田间抗旱性排序
Field drought
resistance ranking		 室内抗旱性排序
Laboratory drought resistance ranking
D11 1 1 D51 11 16
D16 2 3 D40 13 5
D05 3 15 D04 14 18
D37 4 4 D62 15 6
D41 5 19 D24 16 14
D43 6 17 D60 17 11
D18 7 8 D42 18 10
D19 8 7 D22 19 2
D49 9 20 D53 20 21
D14 10 9 D65 21 13
D10 11 12

Fig. 3

Comprehensive evaluation of drought resistance of 21 sorghum accessions at seedling stage based on principal component analysis A: comprehensive scores of drought resistance of 21 sorghum accessions in field test; B: comprehensive scores of drought resistance of 21 sorghum accessions in laboratory test."

Fig. 4

Evaluation of drought resistance of 21 sorghum accessions at seedling stage based on clustering analysis A: clustering analysis results of drought resistance of 21 sorghum accessions in field test; B: clustering analysis results of drought resistance of 21 sorghum accessions in laboratory test."

Fig. 5

Comparison of differences in multilevel phenotypic analysis results of different sorghum drought resistance A: comparison of differences in multilevel phenotypic analysis results between by different methods and in different growth environments; B: comparison of differences in multilevel phenotypic analysis results of sorghum drought resistance under different growth environments. a: comparison between the results of the subordinate function method and the principal component analysis method under field test conditions; b: comparison between the results of the subordinate function method and the principal component analysis method under laboratory test conditions; c: comparison between the results of the principal component analysis in field and laboratory test; d: comparison the results of subordinate function methods between in field and in laboratory test; e: comparison the results between subordinate function method in field test and principal component analysis in laboratory test . The solid lines (0.5 and 1.0) were used as the reference lines for the difference values of c and d to evaluate the differences in the results of multilevel phenotypic analysis with the same analysis method in different growth environments; the dotted lines (1.20 and 1.75) and the solid line (1.5) are used as reference for the e difference value, which is used to assess the difference between the results of subordinate function in field test and the results of the principal component analysis in laboratory test."

[1] Spindel J E, Dahlberg J, Colgan M, Holingsworth J, Sievert J, Staggenborg S, Hutmacher R, Jansson C, Vogel J P . Association mapping by aerial drone reveals 213 genetic associations for Sorghum bicolor biomass traits under drought. BMC Genomics, 2018,19:679. doi: 10.1186/s12864-018-5055-5.
[2] Solomon A D, Hussein S, Mark L, Fentahum M . The impact of drought on sorghum production, and farmer’s varietal and trait preferences, in the north eastern Ethiopia: implications for breeding. Acta Agric Scand, Section B — Soil Plant Sci, 2018,68:424-436.
[3] Sakhi S, Shehzad, Rehman S, Okuno K . Mapping QTL’s underlying drought stress at developmental stages of sorghum (Sorghum bicolor) by association analysis. Euphytica, 2013,193:433-450.
[4] Reddy B V S, Ramaiah B, Kumar A A, Reddy P S . Evaluation of sorghum genotypes for stay-green trait and grain yield. SAT Agric Res, 2007,3:1-4.
[5] Badigannavar A, Teme N, Oliveira A C D, Li G Y, Vaksmann M, Viana V E, Ganapathi T R, Sarsu F . Physiological, genetic and molecular basis of drought resiliencein sorghum [Sorghum bicolor(L.) Moench]. Ind J Plant Physiol, 2018,23:670-688.
[6] 张树林, 刘玉玲, 田丽, 宋卓恒, 郭瑞林, 彭仁海 . 不同小麦新品系萌发期抗旱性的筛选与鉴定. 分子植物育种, 2018,16:7138-7147.
Zhang S L, Liu Y L, Tian L, Song Z H, Guo R L, Peng R H . Screening and identification of drought resistance of different new wheat lines during germination period. Mol Plant Breed, 2018,16:7138-7147 (in Chinese with English abstract).
[7] 郭效龙, 宋希云, 裴玉贺, 郭新梅 . 玉米自交系萌发期和苗期抗旱性指标的筛选. 植物生理学报, 2018,54:1719-1726.
Guo X L, Song X Y, Pei Y H, Guo X M . Screening of drought resistance indexes of maize inbred lines. Plant Physiol J, 2018,54:1719-1726 (in Chinese with English abstract).
[8] 温蕊, 侯建华, 张艳芳, 吕品, 马宇 . 干旱胁迫对向日葵种子萌发的影响及其抗旱性鉴定. 干旱农业地区研究, 2018,36(2):192-197.
Wen R, Hou J H, Zhang Y H, Lyu P, Ma Y . Effect of drought stress on seed germination of sunflower on and identification of drought resistance of main variety. Agric Res Arid Areas, 2018,36(2):192-197 (in Chinese with English abstract).
[9] 陈冰嬬, 徐宁, 李淑杰, 李继洪, 侯佳明, 李伟, 石贵山, 檀辉, 高士杰, 王鼐 . 高粱亲本系萌发期抗旱性鉴定. 中国农业大学学报, 2018,23(8):17-29.
Chen B X, Xu N, Li S J, Li J H, Hou J M, Li W, Shi G S, Tan H, Gao S J, Wang N . Identification of the drought resistance of sorghum parental lines at germination stage. J China Agric Univ, 2018,23(8):17-29 (in Chinese with English abstract).
[10] 吴奇, 周宇飞, 高悦, 张姣, 陈冰嬬, 许文娟, 黄瑞冬 . 不同高粱品种萌发期抗旱性筛选与鉴定. 作物学报, 2016,42:1233-1246.
Wu Q, Zhou Y F, Gao Y, Zhang J, Chen B X, Xu W J, Huang R D . Screening and identification for drought resistance during germination in sorghum cultivars. Acta Agron Sin, 2016,42:1233-1246 (in Chinese with English abstract).
[11] 刘方明, 高玉山, 孙云云, 窦金刚, 刘慧涛, 王立春 . 高粱抗旱性鉴定研究进展. 东北农业科学, 2016,41(3):5-7.
Liu F M, Gao Y S, Sun Y Y, Dou J G, Liu H T, Wang L C . Advances of researches on identification of drought resistance of sorghum. J Northeast Agric Sci, 2016,41(3):5-7 (in Chinese with English abstract).
[12] Azarinasrabad A, Mousavinik S M, Galavi M, Begeshti S A, Sirousmehr A . Evaluation of water stress on yield, its components and some physiological traits at different growth stages in grain sorghum genotypes. Notulae Sci Biol, 2016,8:204-210.
[13] Tambussia E A, Bartolia C G, Beltranoa J, Guiameta J J, Araus J L . Oxidative damage to thylakoid proteins in water-stressed leaves of wheat (Triticum aestivum). Physiol Plant, 2000,108:398-404.
[14] Apel K, Hirt H . Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol, 2004,55:373-399.
[15] Choudhury S, Panda P, Sahoo L, Panda S K . Reactive oxygen species signaling in plants under abiotic stress. Plant Signal Behavior, 2013,8:1559-2324.
[16] 孙欢欢, 韩兆雪, 谭登峰, 张小燕 . 新培育玉米自交系苗期生理生化指标与其抗旱性综合评价. 干旱农业地区研究, 2016,34(5):9-14.
Sun H H, Han Z X, Tan D F, Zhang X Y . Comprehensive evaluation of physiological and biochemical indexes and drought resistance of new maize inbred lines during seedling stage. Agric Res Arid Areas, 2016,34(5):9-14 (in Chinese with English abstract).
[17] Lee D, Chung P J, Jeong J S, Jang G, Bang S W, Jung H, Kim Y S, Ha S, Choi Y D, Kim J . The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance. Plant Biotechnol J, 2016,15:754-764.
[18] 毛浩田, 陈梦莹, 吴楠, 刘海悦, 刘玉林, 张怀渝, 陈洋尔 . 干旱胁迫对不同倍性小麦和八倍体小黑麦苗期光合能力与抗氧化系统的影响. 麦类作物学报, 2018,38:1246-1254.
Mao H T, Chen M Y, Wu N, Liu H Y, Liu Y L, Zhang H Y, Chen Y E . Effects of drought stress on photosynthetic capacity and antioxidant system in wheat with different ploidy levels and octoploid triticale at seedling stage. J Triticeae Crops, 2018,38:1246-1254 (in Chinese with English abstract).
[19] 骆建霞, 黄榆婷, 曾丽蓉, 李钊, 赵嘉菱, 郭冰鑫 . PEG模拟干旱胁迫对4种海棠幼苗生理特性的影响. 天津农学院学报, 2016,23(3):1-5.
Luo J X, Huang Y T, Zeng L Y, Li Z, Zhao J L, Guo B X . Effects of PEG simulated drought stress on physiological characters of four crabapple seedlings. J Tianjin Agric Univ, 2016,23(3):1-5 (in Chinese with English abstract).
[20] 王艺陶, 周宇飞, 李丰先, 依兵, 白薇, 闫彤, 许文娟, 高明超, 黄瑞冬 . 基于主成分和SOM聚类分析的高粱品种萌发期抗旱性鉴定与分类. 作物学报, 2014,40:110-121.
Wang Y T, Zhou Y F, Li F X, Yi B, Bai W, Yan T, Xu W J, Gao M C, Huang R D . Identification and classification of sorghum cultivars for drought resistance during germination stage based on principal components analysis and self organizing map cluster analysis. Acta Agron Sin, 2014,40:110-121 (in Chinese with English abstract).
[21] 孙海博, 任瑞芬, 郭芳, 尹大芳, 杨秀云 . 干旱胁迫下美国薄荷幼苗形态与生理特性研究. 山西农业大学学报(自然科学版), 2018,38(4):65-73.
Sun H B, Ren R F, Guo F, Yin D F, Yang X Y . Research on morphological and physiological responses of Monarda didyma seedlings to drought stress. J Shanxi Agric Univ (Nat Sci Edn), 2018,38(4):65-73 (in Chinese with English abstract).
[22] Mulima E, Sibiya J, Musvosvi C, Nhamucho E . Identification of important morphological traits in Mozambican sorghum [Sorghum bicolor(L.) Moench] germplasm using multivariate analysis. Afr J Agric Res, 2018,13:1796-1810.
[23] Derese S A, Shimelis H, Mwadzingeni L, Laing M . Agro- morphological characterisation and selection of sorghum landraces. Acta Agric Scand, Section B — Soil Plant Sci, 2018,68:585-595.
[24] 汪灿, 周棱波, 张国兵, 徐燕, 张立异, 高旭, 高杰, 姜讷, 邵明波 . 酒用糯高粱资源成株期抗旱性鉴定及抗旱指标筛选. 中国农业科学, 2017,50:1388-1402.
Wang C, Zhou L B, Zhang G B, Xu Y, Zhang L Y, Gao X, Jiang N, Shao M B . Drought resistance identification and drought resistance indices screening of liquor-making waxy sorghum resources at adult plant stage. Sci Agric Sin, 2017,50:1388-1402 (in Chinese with English abstract).
[25] Tufail A, Arfan M, Gurmani A R, Khan A, Bano A . Salcylic acid induced salinity tolerance in maize (Zea mays). Pak J Bot, 2013,45:75-82.
[26] 王兰芬, 武晶, 景蕊莲, 程须珍, 王述民 . 绿豆种质资源苗期抗旱性鉴定. 作物学报, 2015,41:145-153.
Wang L F, Wu J, Jing R L, Cheng X Z, Wang S M . Drought resistance identification of mungbean germplasm resources at seedlings stage. Acta Agron Sin, 2015,41:145-153 (in Chinese with English abstract).
[27] 路文静, 李奕松 . 植物生理学实验教程(第2版). 北京: 中国林业出版社, 2017. pp 132-135.
Lu W J, Li Y S. Plant Physiology Experiment Course (2nd edn). Beijing: China Forestry Publishing House, 2017. pp 132-135(in Chinese).
[28] 王顺才, 邹养军, 马锋旺 . 干旱胁迫对3种苹果属植物叶片解剖结构、微形态特征及叶绿体超微结构的影响. 干旱地区农业研究, 2014,32(3):15-23.
Wang S C, Zou Y J, Ma F W . Influence of drought stress on leaf anatomical structure and micro-morphiology traits and chloroplast ultrastructure of three Malus species. Agric Res Arid Areas, 2014,32(3):15-23 (in Chinese with English abstract).
[29] Chu G, Chen T T, Wang Z Q, Yang J C, Zhan J H . Morphological and physiological traits of roots and their relationships with water productivity in water-saving and drought-resistant rice. Field Crops Res, 2014,162:108-119.
[30] 张嘉楠, 昌小平, 郝晨阳, 刘桂茹, 景蕊莲 . 北方冬麦区小麦抗旱种质资源遗传多样性分析. 植物遗传资源学报, 2010,11:253-259.
Zhang J N, Chang X P, Hao C Y, Liu G R, Jing R L . Genetic diversity of common wheat germplasm resources with drought resistance in Northern China. J Plant Genet Resour, 2010,11:253-259 (in Chinese with English abstract).
[31] Govindasamy V, George P, Aher L, Ramesh S V, Thangasamy A, Anandan S, Raina S K, Kumar M, Rane J, Annapurna J, Annapura K, Minhas P . Comparative conventional and phenomics approaches to assess symbiotic effectiveness of Bradyrhizobia strains in soybean (Glycine max L. Merrill) to drought. Sci Rep, 2017,7:6958. doi: 10.1038/s41598-017-06441-3.
[32] Cheng Z, Dong K, Ge P, Bian Y, Dong L, Deng X, Li X H, Yan Y M . Identification of leaf proteins differentially accumulated between wheat cultivars distinct in their levels of drought tolerance. PLoS One, 2015,10:e0125302.
[33] Wen W, Araus J L, Shah T, Cairns J, Mahuku G, Marianne B, Torres J L, Ciro S, Yan J . Molecular characterization of a diverse maize inbred line collection and its potential utilization for stress tolerance improvement. Crop Sci, 2011,51:2569-2581.
[34] Upadhyaya H D . Variability for drought resistance related traits in the mini core collection of peanut. Crop Sci, 2005,45:1432-1440.
[35] 杨彬, 张一中, 周福平, 张海燕, 史红梅, 张桂香, 柳青山, 张晓娟, 范昕琦 . 高粱抗旱性鉴定方法及QTL定位研究进展. 植物遗传资源学报, 2019,20:496-507.
Yang B, Zhang Y Z, Zhou F P, Zhang H Y, Shi H M, Zhang G X, Liu Q S, Zhang X J, Fan X Q . Advances in experimental assays for drought tolerance and QTL mapping of drought resistance in sorghum. J Plant Genet Resour, 2019,20:496-507 (in Chinese with English abstract).
[36] Houle D, Govindaraju D R, Omholt S . Phenomics: the next challenge. Nat Rev Genetics, 2010,11:855-866.
[1] WANG Xing-Rong, LI Yue, ZHANG Yan-Jun, LI Yong-Sheng, WANG Jun-Cheng, XU Yin-Ping, QI Xu-Sheng. Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage [J]. Acta Agronomica Sinica, 2022, 48(5): 1279-1287.
[2] ZHAO Jia-Jia, QIAO Ling, WU Bang-Bang, GE Chuan, QIAO Lin-Yi, ZHANG Shu-Wei, YAN Su-Xian, ZHENG Xing-Wei, ZHENG Jun. Seedling root characteristics and drought resistance of wheat in Shanxi province [J]. Acta Agronomica Sinica, 2021, 47(4): 714-727.
[3] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[4] WANG Yuan, WANG Jin-Song, DONG Er-Wei, WU Ai-Lian, JIAO Xiao-Yan. Effects of long-term nitrogen fertilization with different levels on sorghum grain yield, nitrogen use characteristics and soil nitrate distribution [J]. Acta Agronomica Sinica, 2021, 47(2): 342-350.
[5] WEI Li-Juan, SHEN Shu-Lin, HUANG Xiao-Hu, MA Guo-Qiang, WANG Xi-Tong, YANG Yi-Ling, LI Huan-Dong, WANG Shu-Xian, ZHU Mei-Chen, TANG Zhang-Lin, LU Kun, LI Jia-Na, QU Cun-Min. Genome-wide association analysis reveals zinc-tolerant loci of rapeseed at germination stage [J]. Acta Agronomica Sinica, 2021, 47(2): 262-274.
[6] DONG Er-Wei, WANG Jin-Song, WU Ai-Lian, WANG Yuan, WANG Li-Ge, HAN Xiong, GUO Jun, JIAO Xiao-Yan. Effects of row space and plant density on characteristics of grain filling, starch and NPK accumulation of sorghum grain of different parts of panicle [J]. Acta Agronomica Sinica, 2021, 47(12): 2459-2470.
[7] ZHANG Rui-Dong,XIAO Meng-Ying,XU Xiao-Xue,JIANG Bing,XING Yi-Fan,CHEN Xiao-Fei,LI Bang,AI Xue-Ying,ZHOU Yu-Fei,HUANG Rui-Dong. Responses of sorghum hybrids to germination temperatures and identification of low temperature resistance [J]. Acta Agronomica Sinica, 2020, 46(6): 889-901.
[8] Li-Ge BAO,Ping LU,Meng-Sha SHI,Yue XU,Min-Xuan LIU. Screening and identification of Chinese sorghum landraces for salt tolerance at germination and seedling stages [J]. Acta Agronomica Sinica, 2020, 46(5): 734-744.
[9] Yin-Ping XU, Yong-Dong PAN, Qiang-De LIU, Yuan-Hu YAO, Yan-Chun JIA, Cheng REN, Ke-Cang HUO, Wen-Qing CHEN, Feng ZHAO, Qi-Jun BAO, Hua-Yu ZHANG. Drought resistance identification and drought resistance indexes screening of barley resources at mature period [J]. Acta Agronomica Sinica, 2020, 46(3): 448-461.
[10] GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan,CUI Cui. Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus [J]. Acta Agronomica Sinica, 2019, 45(9): 1416-1430.
[11] Rui WANG,Liang LING,Peng-Jie ZHAN,Ji-Zhen YU,Jian-Qiang CHU,Jun-Ai PING,Fu-Yao ZHANG. Mapping of genes confessing same height of tiller and main stem in sorghum [J]. Acta Agronomica Sinica, 2019, 45(6): 829-838.
[12] ZHANG Chun-Xiao,LI Shu-Fang,JIN Feng-Xue,LIU Wen-Ping,LI Wan-Jun,LIU Jie,LI Xiao-Hui. QTL mapping of salt and alkaline tolerance-related traits at the germination and seedling stage in maize (Zea mays L.) using three analytical methods [J]. Acta Agronomica Sinica, 2019, 45(4): 508-521.
[13] Qian WANG,Cui CUI,Sang YE,Ming-Sheng CUI,Yu-Feng ZHAO,Na LIN,Zhang-Lin TANG,Jia-Na LI,Qing-Yuan ZHOU. Screening and Comprehensive Evaluation of Germplasm Resources with Tribenuron-methyl Tolerance at Germination Stage in Rapeseed (Brassica napus L.) [J]. Acta Agronomica Sinica, 2018, 44(8): 1169-1184.
[14] Wen-Xue DUAN,Hai-Yan ZHANG,Bei-Tao XIE,Bao-Qing WANG,Li-Ming ZHANG. Identification of Salt Tolerance and Screening for Its Indicators in Sweet Potato Varieties during Seedling Stage [J]. Acta Agronomica Sinica, 2018, 44(8): 1237-1247.
[15] Long LI,Xin-Guo MAO,Jing-Yi WANG,Xiao-Ping CHANG,Yu-Ping LIU,Rui-Lian JING. Drought Tolerance Evaluation of Wheat Germplasm Resources [J]. Acta Agronomica Sinica, 2018, 44(7): 988-999.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd